{"text_format":"3","workbook":"xls","n_sheets":5,"sheets":["Ia_vrn-1 and Cbfs cloning","Ib_3'UTR cloning","Ic_chromosomal localization","Id_RT-PCR","Ie_Cbf GenBank numbers"],"not_rendered":{"external_links":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Ia_vrn-1 and Cbfs cloning","index":0,"n_rows":14,"n_cols":7,"rows_kept":14,"flags":["formulas_cached_only"]}
["Table Ia: List of primers used to clone Scvrn-1 and ScCbfs"]
[]
["Primer set name","Forward primer sequence","Reverse primer sequence","Annealing temperature (°C)","Expected fragment size (bp)","Primer design based on","Cloned sequence Genebank number and name"]
["VRT-1_cloning","ATGGGGCGCGGGAG","ACAGCCGTTGATGTGGCTAAC","51°C",735,"TaVRT-1_AY280870","EU525891_Scvrn-1"]
["act_cloning","CCAGCAATGTATGTCGCAATC","GCCAGCAAGGTCCAAACG","55°C",159,"TaActin_AB181991","EU525892_ScActin"]
["CBF23_cloning","ATGGAGTGGGCGTACAGCGG","GTCAGTAGTTCCACAGGCTGA","54.9°C",657,"TaCBFIa-A11_EF028751 (complete CDS)","EU194240_ScCbfIa-11"]
["CBF28_cloning","ATGGACCAGTACAACTACGG","GTCAGTAGCTCCAGAGCGCGA","60.7°C",687,"TaCBFII-5.3_EF028754 (complete CDS)","EU194241_ScCbfII-5"]
["CBF29_cloning","ATGTGTCCGATCAAGAGGGA","CTAGCTCTGGTAGCTCCAGA","53.4°C",717,"TaCBFIIIa-D6_EF028757 (complete CDS)","EU194242_ScCbfIIIa-6"]
["CBF30_cloning","ATGGACATGGGCCTTGAGGT","CTAGTAGCTCCAGAGCGCGA","58.1°C",738,"TaCBFIIIc-D3_EF028760 (complete CDS)","EU194244_ScCbfIIIc-3A e EU194245_ScCbfIIIc-3B"]
["CBF31_cloning","ATGGACATGGGCCTTGAGG","CTAGCTCCAGAGCGCGAACT","56.5°C",723,"TaCBFIIIc-B10_EF028761 (complete CDS)"," EU194243_ScCbfIIIc-10"]
["CBF32_cloning","ATGGACACGGGCCCCGAGCG","GTCAGTGGTTCCATAGCGCCG","56.5°C",738,"TaCBFIIId-B12_EF028763 (complete CDS)","EU194246_ScCbfIIId-12"]
["CBF33_cloning","ATGGACATGACCGGCTCCGA","GTTAGTAGCTCGAGAGCGCGG","62°C",726,"TaCBFIIId-15.2_EF028765 (complete CDS)","EU194247_ScCbfIIId-15"]
["CBF34_cloning","ATGGACATGGGCATCAACGG","GTCAGTAGCTCCAGAGGCCGG","60.7°C",705,"TaCBFIIId-D19_EF028768 (complete CDS)","EU194248_ScCbfIIId-19"]
["CBF35_cloning","GGACGGTGTGGTCGGAGCCG","GTTACTCTCTGTTCTTCCCAT","50.2°C",695,"TaCBFIVa-2.2_EF028770 (partial CDS)","EU194249_ScCbfIVa-2A e EU194250_ScCbfIVa-2B"]

{"sheet":"Ib_3'UTR cloning","index":1,"n_rows":17,"n_cols":7,"rows_kept":17,"merged":[[6,1,7,1],[6,2,7,2],[6,4,7,4],[9,1,10,1],[9,2,10,2],[12,1,13,1],[12,2,13,2],[12,4,13,4],[14,1,15,1],[14,2,15,2],[14,4,15,4]],"flags":["formulas_cached_only"]}
["Table Ib: List of primers used to clone 3'UTR of ScCbfs"]
[]
["Primer set name","Forward primer sequence","Primer design based on","Reverse primer sequence","Primer design based on","Annealing temperature °C","Expected fragment size (bp)"]
["T17-ADAPTER",null,"/","GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTT","/"]
["ADAPTER reverse",null,"/","GACTCGAGTCGACATCG","/","55°C"]
["3'UTRCBF32","GTCTCACTCGCAGATGCC","EU194246_ScCbfIIId-12",null,"/","55°C"]
["3'UTRCBF23_3","CATGGATGGACTGGAATTC","EU194240_ScCbfIa-11","AGCCGGCTCAGGTGCGC","TaCBFIa-A11","58°C",159]
["3'UTRCBF23_4",null,null,"TGCCAAGATCGCCCGTGC",null,"58°C",334]
["3'UTRCBF28_3","CCCGATGATGGCGATGAT","EU194241_ScCbfII-5","TGATGTTGTACAGCAGCCCCATC","TaCBFII-5.1","58°C",298]
["3'UTRCBF29_3","GAGGCCGAGGACTCGC","EU194242_ScCbfIIIa-6","TCGATCGGAAGCCAAGACGAC","TaCBFIIIa-6.2","58°C",239]
["3'UTRCBF29_4",null,null,"AATTCGCACCATCACATTACCAGA","TaCBFIIIa-6.1","58°C",305]
["3'UTRCBF30_4","ACACGAGAGGTTGCCAAC","EU194244_ScCbfIIIc-3A","GTTGAGTGCTGAATTAGGAGCAGC","TaCBFIIIc-3.1","58°C",390]
["3'UTRCBF31_3","GCCGATGGAGAGTTCGC"," EU194243_ScCbfIIIc-10","CAGAGTCAAAACAAAAGCAGAATC","TaCBFIIIc-B10","58°C",245]
["3'UTRCBF31_4",null,null,"CGAAATTGGAAAACTTTGCAAAGG",null,"58°C",283]
["3'UTRCBF33_3","ACGTCGTCGTCCCTATCT","EU194247_ScCbfIIId-15","GCTGACAGGGAACAGCTTCGG","TaCBFIIId-A15","58°C",281]
["3'UTRCBF33_4",null,null,"GCGATGTCCTTTCGTGTGTCG",null,"58°C",391]
["3'UTRCBF34_4","GCAATGCCGACGAAGTGT","EU194248_ScCbfIIId-19","GGAGCAGTTTGCAGTTGGTCC","TaCBFIIId-A19","58°C",280]

{"sheet":"Ic_chromosomal localization","index":2,"n_rows":17,"n_cols":7,"rows_kept":17,"flags":["formulas_cached_only"]}
["Table Ic: List of primers used for chromosomal localization of ScCbfs and Scvrn-1"]
[]
["Primer set name","Forward primer sequence","Reverse primer sequence","Primer design based on","Annealing temperature °C","Expected fragment size (bp)","Restriction enzyme digestion of PCR fragments*"]
["Vrn1_2","AAGAAGGAGAGGTCACTGCAGG","GGTATTTCCGGCAAGGGCAG","EU525891-ScVrn-1",56,250,"NA"]
["ScCbf23_exp","TGCCGAGGCGCCGGCTGCTTCG","AATTCCAGTCCATCCATGAGGCAGT","EU194240_ScCbfIa-11",55,91,"NA"]
["ScCbf29_exp","GGCGGAGGCCGAGGCCGAGGAC","CCGCCGCGGAGGGCGGCGCCA","EU194242_ScCbfIIIa-6",55,119,"NA"]
["ScCbf31_exp","GAATGGACATCGAAATGCTCAGGC","GCGCCGGTGCCGGTCGCCACCGG"," EU194243_ScCbfIIIc-10",55,121,"NA"]
["Scclone79(IV)_exp","ACACGCCGCTTTGGAACTAGTA","GGGAAAATTGCTCCTTATGCTTACT","AF370728",55,137,"NA"]
["Scclone80(IV)_exp","GACCTGTTGGAGCTCGACGA","CGGGAACTCTACTCTGCTTCTTCC","AF370730",55,244,"NA"]
["Scclone81(IV)_exp","AGACGCCGCTATGGAGCTGT","GGAGTGCTGCTGTACAATGAAGG","AF370729",55,128,"NA"]
["ScCbf28_exp","CAGCTACGCCATGTACGGCGGC","GTTCATGTGGGAGATACTCTGGCAG","EU194241_ScCbfII-5",55,112,"TseI, Sc, 70+39+3"]
["ScCbf30_exp","TCGCAGTGGACACCGATATGTTCGG","CACGTCGTTGGTGGTCACCGGTG","EU194244_ScCbfIIIc-3A",55,125,"BsrI, Ta, 76+49"]
["ScCbf32_exp","GAGCTCGGAGCACGGAGATGAT","TGATAGGTCCACCGTCCACG","EU194246_ScCbfIIId-12",55,153,"MboI, Sc, 89+64"]
["ScCbf33_exp","GTCGTCGTCCCTATCTTCTGGCGAC","TCAGTGTCCCAGCACGCGCCGACGA","EU194247_ScCbfIIId-15",55,180,"XhoI, Ta, 134+46"]
["ScCbf34_exp","CCGTGGGCAATGCCGACGAAGTGT","CGTTCTCCCAGTACGCCGGCGTGG","EU194248_ScCbfIIId-19",55,168,"MboI, Sc, 90+78"]
["ScCbf35_exp","GCCGAGGAGAGCGCGCTCTCCATC","CGTGTCGAATCCGTCGTCGTGTTC","EU194249_ScCbfIVa-2A",55,180,"RsaI, Ta, 92+88"]
["* NA, Not applicable; Restriction enzyme name, specificity (Ta=wheat, Sc=rye), expected fragment band size (bp) after digestion."]

{"sheet":"Id_RT-PCR","index":3,"n_rows":31,"n_cols":6,"rows_kept":31,"flags":["formulas_cached_only"]}
["Table Id: List of primers used for RT-PCR analysis"]
[]
["Primer set name","Forward primer sequence","Reverse primer sequence","Primer design based on","Annealing temperature °C","Expected fragment size (bp)"]
["act_exp","CCAGCAATGTATGTCGCAATC","GCCAGCAAGGTCCAAACG","AB181991","55°C",159]
["ScCBF23_exp","TGCCGAGGCGCCGGCTGCTTCG","AATTCCAGTCCATCCATGAGGCAGT","EU194240","55°C",91]
["ScCBF30_exp","TCGCAGTGGACACCGATATGTTCGG","CACGTCGTTGGTGGTCACCGGTG","EU194244","55°C",125]
["ScCBF31_exp","GAATGGACATCGAAATGCTCAGGC","GCGCCGGTGCCGGTCGCCACCGG"," EU194243","55°C",121]
["ScCBF32_exp","GAGCTCGGAGCACGGAGATGAT","TGATAGGTCCACCGTCCACG","EU194246","55°C",153]
["ScCBF33_exp","GTCGTCGTCCCTATCTTCTGGCGAC","TCAGTGTCCCAGCACGCGCCGACGA","EU194247","55°C",180]
["ScCBF34_exp","CCGTGGGCAATGCCGACGAAGTGT","CGTTCTCCCAGTACGCCGGCGTGG","EU194248","55°C",168]
["ScCBF35_exp","GCCGAGGAGAGCGCGCTCTCCATC","CGTGTCGAATCCGTCGTCGTGTTC","EU194249","55°C",180]
["Scclone79(IV)_exp","ACACGCCGCTTTGGAACTAGTA","GGGAAAATTGCTCCTTATGCTTACT","AF370728","55°C",137]
["Scclone80(IV)_exp","GACCTGTTGGAGCTCGACGA","CGGGAACTCTACTCTGCTTCTTCC","AF370730","55°C",244]
["Scclone81(IV)_exp","AGACGCCGCTATGGAGCTGT","GGAGTGCTGCTGTACAATGAAGG","AF370729","55°C",128]
["TaCBFII-5.2_exp","CTTCATCTCGATTGGCTTGCTC","CGTTCTCTCTCTGCTTTACGAACAC","EF028753","55°C",139]
["TaCBFIIId-B12_exp","ATGATGCGGCGCTATGGAA","CGGCGCTTTTTCGGAGTAAAT","EF028763","55°C",111]
["TaCBFIVc-B14_exp","CACCAGACCGTGGCGGGAAG","CCCATATTTGGTGGAACAGAAGAGA","EF028778","55°C",132]
["HvCBF1(I)_exp","ACTGATTACGCGCATTTGAGTTATG","GAAAAGGAAAGGGCAACCATG","AY785837","55°C",117]
["HvCBF6(II)_exp","CGACATGGGCTGGGACCTTT","CGAACTAGCTTGCTAGCTCTGGTAG","AY785860","55°C",151]
["HvCBF2A(IV)_exp","CGAACACGACGACGGCTTCA","ACGCCTGGTGGAAGAACACAGC","AY785841","58°C",130]
["wcs120_exp","CGTTGGAGGAGAGAATGTATTATG","CTGCGTCTGTCTCTTGGATAAG","AF031235","55°C",215]
["wcor14b_exp","GAGAAGGCGAAGCAGGCGAC","TTGCTCACATCCTCAACCGC","AF207546","58°C",100]
["wcor410_exp","GTTGTGTTTGCTTTACGTCTAAGTT","ACAACATCCAGGACCTGACTCTG","L29152","55°C",135]
["wcs19_exp","TAGTCCGATCTGTATCGCTCAATTC","GCGGTAATCCTATATCACAGAAAAT","L13437","55°C",111]
["Hvdhn5_exp","CCACCAGCATACCACTGAGACC","TAGTGCTGTCCAGGCAGCTTGT","AF043096","55°C",207]
["Hvcor14b_exp","GAGAAGGCGAAGGAGGCGGC","CAAAGGGAACAAGATCGCC","AJ512944","60°C",254]
["Hvdhn8_exp","TGCTCCAGCGCCAGTGCAC","CGATCAAGCTCTGGGCTTGTG","AF181458","55°C",178]
["REP13_exp","GAGAACGTGAAGGGGGCGGC","TTGCTCACATCGTCAACCGC","AF491839","55°C",242]
["REP14_exp","GCGAGCGAAGTGGACGACAA","TTGTCCGTGATGCCCTGTGC","AF491840","55°C",107]
["TC3078_exp","GTTGTGTTTGCTTTACGTCTAAGTT","GTAAACAAACAGGACCTGACTCTG","TC3078 (COR410 homologue)","55°C",157]
["TC2696_exp","GCGCGCTACCTTTCCAGAATA","ACCAAGATACATAACCCACTCG","TC2696(COR39 homologue)","55°C",107]

{"sheet":"Ie_Cbf GenBank numbers","index":4,"n_rows":56,"n_cols":4,"rows_kept":56,"flags":["formulas_cached_only"]}
["Table Id: List of primers used for RT-PCR analysis"]
[]
["Name","GB accession number","species","reference"]
["TaCbfIa-A11","EF028751","Triticum aestivum","Badawi et al, 2007"]
["TaCbfII-5.1","EF028752","Triticum aestivum","Badawi et al, 2007"]
["TaCbfII-5.2","EF028753","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIIIa-D6","EF028757","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIIIc-D3","EF028760","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIIIc-B10","EF028761","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIIId-B12","EF028763","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIIId-A15","EF028764","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIIId-D19","EF028768","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIVa-A2","EF028769","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIVb-D21","EF028776","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIVb-A20","EF028772","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIVc-B14","EF028778","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIVd-B4","EF028781","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIVd-A22","EF028785","Triticum aestivum","Badawi et al, 2007"]
["TaCbfIVd-B9","AF028783","Triticum aestivum","Badawi et al, 2007"]
["HvCbf1","AY785837","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf11","AY785890","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf5","AY785855","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf10A","AY785882","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf3","AY785845","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf13","DQ095158","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf12","DQ095157","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf6","AY785860","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf2A","AY785841","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf14","DQ095159","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf4A","AY785849","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf9","AY785878","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["TmCbf5","AY951947","Triticum monococcum","Miller et al, 2006"]
["TmCbf10","AY951950","Triticum monococcum","Miller et al, 2006"]
["TmCbf3","AY951949","Triticum monococcum","Miller et al, 2006"]
["TmCbf13","AY951951","Triticum monococcum","Miller et al, 2006"]
["TmCbf18","AY951946","Triticum monococcum","Miller et al, 2006"]
["TmCbf15","AY951944","Triticum monococcum","Miller et al, 2006"]
["TmCbf16","AY951944","Triticum monococcum","Miller et al, 2006"]
["TmCbf17","AY951945","Triticum monococcum","Miller et al, 2006"]
["TmCbf12","AY951944","Triticum monococcum","Miller et al, 2006"]
["TmCbf2","AY951945","Triticum monococcum","Miller et al, 2006"]
["TmCbf14","AY951948","Triticum monococcum","Miller et al, 2006"]
["TmCbf4","AY951945","Triticum monococcum","Miller et al, 2006"]
["TmCbf9","AY951945","Triticum monococcum","Miller et al, 2006"]
["HvCbf1","AY785837","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf11","AY785890","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf5","AY785855","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf10A","AY785882","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf3","AY785845","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf13","DQ095158","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf12","DQ095157","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf6","AY785860","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf2A","AY785841","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf14","DQ095159","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf4A","AY785849","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]
["HvCbf9","AY785878","Hordeum vulgare subsp. vulgare","Skinner et al, 2005"]