Source data for Figure 1: The changes in the concentration of the intracellular and extracellular metabolites (uM) and the protein abundances or phosphorylation levels of the enzymes (A.U.; arbitrary units) in response to an insulin step stimulatiojn in FAO cells. See "Metabolome Analysis" and "immunoblotting" in Materials and Methods for the calculation of the metabolite concentrations and the normalization of the enzymes. In the column headers, for example, "G6P_1nM" means the changes in the concentration of G6P in response to 1 nM of insulin step stimulation. Time G6P_0nM G6P_1nM G6P_100nM F16P_0nM F16P_1nM F16P_100nM DHAP_0nM DHAP_1nM DHAP_100n 3PG_0nM 3PG_1nM 3PG_100nM DPG_0nM DPG_1nM DPG_100nM PEP_0nM PEP_1nM PEP_100nM LAC_0nM LAC_1nM LAC_100nM G1P_0nM G1P_1nM G1P_100nM UDPG_0nM UDPG_1nM UDPG_100nM Glycogen_0nM Glycogen_1nM Glycogen_100nM PYRex_0nM PYRex_1nM PYRex_100nM GLCex_0nM GLCex_1nM GLCex_100nM GK_0nM GK_1nM GK_100nM G6Pase_0nM G6Pase_1nM G6Pase_100nM PEPCK_0nM PEPCK_1nM PEPCK_100nM pGS_0nM pGS_1nM pGS_100nM pGP_0nM pGP_1nM pGP_100nM pAKT_0nM pAKT_1nM pAKT_100nM min uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM uM FoldChange FoldChange FoldChange uM uM uM uM uM uM A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. A.U. 0 2.744893124 NaN 4.420574048 11.63165523 9.803883167 9.703864194 60.675708 65.48846992 51.01135413 151.9840699 153.6557398 145.0696168 6.616915479 3.250356025 5.763884564 91.2759721 89.22968005 80.05778694 844.6770391 941.4339367 1493.587009 6.513923432 5.221048644 5.534147035 96.62400575 84.81860753 76.5858839 1 1 NaN 132.7956697 171.0756281 142.8915577 119.2264807 119.2264807 119.2264807 0.553782073 0.553782073 0.553782073 0.646624336 0.646624336 0.646624336 0.622424967 0.622424967 0.622424967 0.657606777 0.657606777 0.657606777 0.172333702 0.172333702 0.172333702 0.027435966 0.027435966 0.027435966 10 NaN NaN NaN NaN 13.2495425 17.12774081 NaN 85.72465371 83.0613968 NaN 220.6597465 249.1021329 NaN 7.068801664 13.8584122 NaN 129.179352 135.7995306 NaN 1493.302175 1228.238879 NaN 7.329351964 6.024237115 NaN 83.90486425 77.11900427 NaN NaN NaN NaN 154.0525252 157.5576455 118.2088734 118.7490046 110.6845162 0.606166848 0.58258742 0.553542613 0.48205409 0.410228525 0.441743561 0.631888369 0.609976127 0.584495645 0.774004789 0.696673963 0.687484757 0.204732194 0.221958985 0.325936448 0.02517691 0.548315275 0.929361202 30 5.418247796 NaN NaN 6.80471228 11.09381888 8.203603593 65.92515837 63.3168614 73.94687867 143.9473607 169.035609 195.0384514 NaN 8.384469014 7.146597915 92.93646861 104.489017 119.3442034 1122.617673 1680.021354 1362.463058 6.96262809 6.029864071 6.032752402 99.55580444 97.17471435 92.68867566 NaN NaN NaN 156.9370718 167.3196677 181.9169954 116.2228473 111.9450199 110.9608556 0.595439797 0.613716321 0.589586202 0.482211203 0.391057231 0.512625011 0.639138319 0.641690751 0.652396157 0.745598701 0.804409188 0.807640476 0.112714734 0.224557982 0.324018448 0 0.509800201 0.922872737 60 3.856962387 NaN NaN 6.662042174 6.232876627 5.901741591 55.93543224 63.12292751 56.45615055 123.7765529 160.8089014 160.8363805 4.581855199 7.519916673 6.656288785 82.81453018 92.61757951 101.8971416 1046.738329 1278.017813 1492.187587 5.174550658 6.415782191 8.412169033 90.79829889 93.04770804 99.6667103 0.740888719 1.566748778 NaN 147.8442738 146.6661419 147.7899385 115.0368892 108.7111488 114.2945309 0.638988482 0.65345815 0.69349038 0.347200127 0.283566035 0.434338817 0.671525566 0.731143316 0.666229429 0.713384226 0.795620124 0.774826234 0.218837538 0.217002916 0.222546049 0 0.316563785 0.750606324 120 NaN NaN 2.970039001 7.813883764 6.761473935 15.57115926 57.67921239 54.72300904 47.68898102 158.7269055 167.5386951 161.295031 3.47242617 3.85470785 5.120084132 85.06000971 104.6689459 87.85118933 824.214592 1323.93379 1766.589315 4.921136374 7.673199864 7.252830503 94.65131396 107.3642626 112.1954052 0.753853297 2.036748032 NaN 146.4384148 111.1849252 139.2552117 106.8046723 102.4356464 98.42682883 0.603872794 0.67989369 0.665060794 0.386596094 0.299754217 0.40144281 0.65601318 0.653859143 0.611833062 0.821431907 0.778897592 0.732715181 0.183035461 0.251668458 0.257668495 0 0.280407187 0.692582102 240 NaN NaN NaN 7.095410329 4.697941211 4.587710482 47.73802522 41.62646633 38.49064989 152.956397 149.2512742 172.1602346 2.853306967 2.700457578 3.33928473 95.08601507 89.01471137 104.6515587 1078.613863 1518.566411 1935.960548 5.295562486 5.406769426 4.515011581 97.99497182 77.26056333 85.03613057 0.362567537 0.506361583 NaN 94.77679692 103.2839105 109.1089786 112.0143963 88.89072437 84.6788907 0.646324724 0.640930296 0.707273521 0.437492396 0.346005163 0.475439569 0.79880635 0.656709566 0.640115116 0.852859843 0.868099664 0.894138106 0.216456342 0.342632121 0.387734919 0.019092667 0.240389221 0.611119937 360 4.438701368 NaN NaN 10.28900459 4.604748579 4.223513449 39.58811941 32.8306513 29.96663128 122.8896266 114.1361053 125.2303646 1.57939302 3.064727668 2.282127014 66.97184569 60.99440695 62.79717682 1726.579573 1431.05415 2347.331447 4.923586891 3.221753603 2.63117754 79.5000064 67.15807994 61.32757763 0.535182481 0.649538943 NaN 74.28849787 55.13341664 73.92125286 108.9186165 62.98650797 57.09089529 0.67158104 0.692210533 0.623955193 0.554776344 0.363902147 0.495826101 0.916587729 0.485603772 0.558684627 0.857959121 0.731904934 0.787659698 0.280794024 0.415222392 0.554708118 0 0.236277069 0.507955803 480 6.230425969 NaN NaN 8.951543785 4.038971328 1.637878452 42.16332594 23.96709057 17.25603722 120.1360033 103.9341487 100.2317433 2.342496558 1.933098028 NaN 69.1066124 52.64683474 53.54281043 1577.818432 1961.530228 1767.870616 5.176103611 NaN NaN 83.74046658 55.52882673 41.23702202 0.309923918 0.348525896 NaN 44.21226907 56.41162175 50.70116203 98.90083224 44.2449323 38.60988666 0.807629564 0.848202626 0.737549685 0.660526707 0.505478719 0.576551852 0.835291683 0.373762751 0.405138327 0.780882349 0.742475166 0.829620263 0.285010626 0.75421708 0.814575329 0 0.362132374 0.716382351