{"text_format":"3","workbook":"xls","n_sheets":3,"sheets":["Sheet1","Sheet2","Sheet3"],"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Sheet1","index":0,"n_rows":118,"n_cols":7,"rows_kept":118,"flags":["formulas_cached_only"]}
[null,"Primers"]
[null,"Enard et al. „Molecular evolution of FOXP2, a gene involved in speech and language“"]
[]
["Primer name","Primer sequence 5' to 3'","orientation","Start","End",null,"orientation, start and end positions refer to the completly "]
[{"$nulls":6},"sequenced BAC clone with accession number AC020606"]
[null,"Primers for amplifying large fragments"]
["hCAGH44-L3","AAAGCTAGTGTCTATGTTTTGTGATAC","upper",31714,31740]
["hCAGH44-L2","AGAACTGAAGACTGTCCATTCACTAT","lower",45966,45941,null,"PCR conditions for amplifying large fragments"]
["SpchXL27","CAGCTTCTCCAGATGCAACAACTCC","upper",34949,34973,null,"according to manufactures protocol; reaction in 25µl "]
["SpchXL21","AAAGCTGTGTTGAAACTAGCGTAGACAT","upper",40095,40122,null,"92°, 2‘15“; [92°, 10“; 60°, 30“; 68°, 11‘30“] 11x; [92°, 15“; 60°, 30“; 68°, 11‘30“ + 10“/cyclus] 21x; 68° 7‘"]
["SpchXL28","ACTCAAACTACCTCTCCCAACCGAACA","lower",44090,44116]
[]
[null,"Primers used for Reamplification",{"$nulls":4},"PCR conditions for Reamplification"]
["SpchRea21","ATCAGGAGTATCGTGGTAATG","upper",35997,36017,null,"per 50µl PCR reaction we used 2µl of the template PCR reaction, 1U Taq Gold (Perkin Elmer),"]
["SpchRea21or","ATCAGGAGTATCATGGTAATG","upper",35997,36017,null,"standard buffer (Perkin Elmer), 10 pmol of each primer, 200µM dNTPs (Pharmacia)"]
["SpchRea17","TACATCTTGAGAGGAAGACTC","upper",39129,39149,null,"94°, 6‘; [94°, 45‘‘; 57°-60°, 45‘‘; 72°, 1‘30‘‘ to 3‘ 15“ "]
["SpchRea22or","TGTCACCTACAGATACAATGCC","lower",39437,39458,null,"depending on product length (0.6 to 3.5 kbp)] 35 cycles; 72° 3‘;"]
["SpchRea22","ATCACCTACAGATACAATGCC","lower",39437,39457]
["SpchRea19","CACTTAGAAGAATGTCTGCCA","upper",41312,41332]
["SpchRea18or","CAGGTGTAAGCTTCGGAGTTA","lower",41531,41551]
["SpchRea18","CAGGTGTAAACTTAGGAGTCA","lower",41531,41551]
["SpchRea7","TGTTGGGACACCTAACATAC","upper",43518,43537]
["SpchRea20","GCTATGCCTATCACAATTTCG","lower",43805,43825]
["SpchRea12","ATGATGAGTTATTGGTGGTG","lower",45937,45918]
[]
[null,"Primers used for Reamplification and Sequencing"]
["hCAGH44-1","ATGATGACTCCCCAGGTGAT","upper",31919,31938]
["hCAGH44-5","CTCATCGTTTAGAAAGAGAAGAAT","upper",32489,32512]
["hCAGH44-2","TGGCTCAGCTGCCTAATTTC","lower",32546,32527]
["hCAGH44-7","TATGTAGAGCTGTCTCTTTGAATC","upper",33005,33028]
["hCAGH44-6","CATATGGCAGAGTATCCATTAAGA","lower",33063,33086]
["hCAGH44-9","ACTGAGCTTAGTAACAGTGATAG","upper",33495,33517]
["hCAGH44-8A","TGAGAGCCTATCACTGTTAC","lower",33505,33524]
["hCAGH44-8","AACATTAGACGACAGCAGCATTAG","lower",33565,33588]
["hCAGH44-11","TATGCAAACGTACAGGAAACAGT","upper",34006,34028]
["hCAGH44-10","AGTTGATGGCTGTCTGAAATTCT","lower",34070,34092]
["hCAGH44-13","TGGACCTCTGCAGATCAAACTTA","upper",34491,34513]
["hCAGH44-12A","CCAATTGAATAAGTTTGATC","lower",34503,34522]
["hCAGH44-13A","AAGTGTGTCGTAGAAGTTGA","upper",34534,34553]
["hCAGH44-12","ATACATTTCTCTAGATTAAAAGAAC","lower",34572,34596]
["hCAGH44-3","TTGGCAGCCCAGCAGCTTGT","upper",34919,34938]
["hCAGH44-15","AGCAGCATCTGCTCAGCCTTCA","upper",34980,35001]
["hCAGH44-14","CCAGGTGGAATGGAGATGAGTC","lower",35010,35031]
["hCAGH44-17","GATCTGCAGGGAATTGGATTAC","upper",35373,35394]
["hCAGH44-16A","ACTCCTATCAGCTCACTTAG","lower",35422,35441]
["hCAGH44-16","TTTTATCAGTTGTCCACCAAACTC","lower",35438,35461]
["hCAGH44-19","GCTAAAATTCTCAATTCAGGTAAG","upper",35798,35821]
["hCAGH44-18","TGTTTGTCCCAACAAAATCCAAAT","lower",35863,35886]
["hCAGH44-20","ACATTCATTGATATAATGACGCTA","lower",36244,36267]
["Spch_ABI-15","GTGATTCACCCACCTCAG","upper",45403,45420]
["Spch_ABI-20","TGTTCTTTTTCATTGTCTCA","lower",46061,46042]
[]
[null,"Primers used for Sequencing"]
["Spch_ABI-57","AGTATCGTGGTAATGTTCTC","upper",36003,36022]
["Spch_ABI-57or","AGTATCATGGTAATGTTCTC","upper",36003,36022]
["Spch_ABI-59","AGCATGATCTACCACAATC","upper",36483,36501]
["Spch_ABI-58","AGATTGTGGTAGATCATGC","lower",36484,36502]
["Spch_ABI-60","ACTACATTTGTGGCCCTAC","lower",36924,36942]
["Spch_ABI-61","CCATGTAAGCCACACAAAC","upper",36972,36990]
["Spch_ABI-72","AAGATACAGTTTTAAAGACAC","lower",37343,37363]
["Spch_ABI-69","TTTTGAGGAGTCTCACTCTG","upper",37373,37392]
["Spch_ABI-62","TAAGTTAGCGTCACTGCAC","lower",37407,37425]
["Spch_ABI-63","GAGTAGCTGCAATTACAGGTG","upper",37482,37502]
["Spch_ABI-64","GAGATGGAAGTTTACTTGCCT","lower",37917,37937]
["Spch_ABI-65","CAAGTAAACTTCCATCTCC","upper",37920,37938]
["Spch_ABI-66","ATCCAGGCTAGAATCTAGC","lower",38391,38409]
["Spch_ABI-67","TCTAGCCTGGATTCTTCTG","upper",38398,38416]
["Spch_ABI-68","ATTCACAGGTAGCTTGGCAG","lower",38883,38902]
["Spch_ABI-35","TGAGAGGAAGACTCGAAAG","upper",39136,39154]
["Spch_ABI-70","CTACAGATACAATGCCTATC","lower",39433,39452]
["Spch_ABI-70or","CTACAGATACAATGCCCATC","lower",39433,39452]
["Spch_ABI-51","TCTGTAGGTGATAGTAGAC","upper",39446,39464]
["Spch_ABI-36","ACTAGAGGTCTACTATCAC","lower",39453,39471]
["Spch_ABI-37","ACATAGTAATATCTCAGTG","upper",39722,39740]
["Spch_ABI-38","GTGATACGCTACTTATTTTC","lower",39846,39865]
["Spch_ABI-74","TCATTTCTTTTTCTGTGTCC","lower",39953,39972]
["Spch_ABI-71","TGAGTGTAAAAAAGCTGCC","upper",40000,40018]
["Spch_ABI-53","TGTTGAAACTAGCGTAGAC","upper",40102,40120]
["Spch_ABI-40","ATAGGGTCTCACTATGTTG","lower",40347,40365]
["Spch_ABI-39","AGTGAGACCCTATCTTTAC","upper",40353,40371]
["Spch_ABI-42","TATAAGAGTGCCTGCAGTC","lower",40923,40941]
["Spch_ABI-41","ATAGACACTGGGGATATAG","upper",40939,40957]
["Spch_ABI-43","GAAGAATGTCTGCCATATCA","upper",41318,41337]
["Spch_ABI-44","AAACTTAGGAGTCACCAAC","lower",41526,41544]
["Spch_ABI-44or","AAGCTTCGGAGTTACCAAC","lower",41526,41544]
["Spch_ABI-45","CCAAGAGCAAGGTAATAGT","upper",41964,41982]
["Spch_ABI-56","ACTATTACCTTGCTCTTGG","lower",41964,41982]
["Spch_ABI-46","ATTCCTTTTAGCGGCAGTG","lower",42166,42184]
["Spch_ABI-46_1","AGATAAGAGGCAGAGAATA","lower",42199,42217]
["Spch_ABI-46_1or","AGATAAGAGGCAGATAATA","lower",42199,42217]
["Spch_ABI-47","GTTAGCCACTCAATCTATG","upper",42538,42556]
["Spch_ABI-54","CATAGATTGAGTGGCTAAC","lower",42538,42556]
["Spch_ABI-48","CTTGTTATCACCAGTCTCC","lower",42815,42833]
["Spch_ABI-49","TAGATGAGATCAGAGAGAC","upper",43134,43152]
["Spch_ABI-49chp","TAGATGAGATCAGAAAGAC","upper",43134,43152]
["Spch_ABI-50","ACTTTCACCATCTTACCAG","lower",43440,43458]
["Spch_ABI-1","ACACCTAACATACACCAAC","upper",43525,43543]
["Spch_ABI-52","GCCTATCACAATTTCGTAC","lower",43802,43820]
["Spch_ABI-3","GAAATTGTGATAGGCATAGC","upper",43806,43825]
["Spch_ABI-2","CTAAGTATTGGCCAGTAGC","lower",43904,43922]
["Spch_ABI-5","GAGAGGTAGTTTGAGTTGC","upper",44101,44119]
["Spch_ABI-4","TGGGCAGAATTCAAACTC","lower",44194,44211]
["Spch_ABI-7","CAAATATACTCAAGCCAGAC","upper",44426,44445]
["Spch_ABI-6","CAGCTTCTACGTGGTAAAG","lower",44466,44484]
["Spch_ABI-9","AAATAAATTTGCATAACCTG","upper",44737,44756]
["Spch_ABI-8","TTTAAACAATTCCCTGATTC","lower",44756,44775]
["Spch_ABI-11","CTGATCCAGTTAAGTAAGC","upper",44839,44857]
["Spch_ABI-10","ATCAGTGAGGAACTGAAAG","lower",45048,45066]
["Spch_ABI-13","ACCTTAATACTAGCAATAGG","upper",45077,45096]
["Spch_ABI-12","AAAATGAGCTGGGTGTG","lower",45321,45337]
["Spch_ABI-15","GTGATTCACCCACCTCAG","upper",45403,45420]
["Spch_ABI-14","GAGTACCAATTTGGGAATAG","lower",45556,45575]
["Spch_ABI-17","ACATCACTGTTGTTGTTGG","upper",45667,45685]
["Spch_ABI-17chp","ACATCACTGTCGTTGTTGG","upper",45667,45685]
["Spch_ABI-19","TGAGATTCAGCAGTTATG","upper",45787,45804]
["Spch_ABI-16","AGTTATTGGTGGTGATGC","lower",45931,45914]
["Spch_ABI-16chp","AGTTATTGGTGGTGACGC","lower",45931,45914]
["Spch_ABI-20","TGTTCTTTTTCATTGTCTCA","lower",46061,46042]

{"sheet":"Sheet2","index":1,"n_rows":0,"n_cols":0,"rows_kept":0,"flags":["formulas_cached_only"]}

{"sheet":"Sheet3","index":2,"n_rows":0,"n_cols":0,"rows_kept":0,"flags":["formulas_cached_only"]}