1.	System requirements. The code has been tested using R4.0.1 on Windows 7 (64-bit).
2.	Install R4.0.1 and RStudio 1.3.1093.
3.	Open RStudio and load the code file.
4.	Prepare CSV data files in Excel. Each row represents a patient and each patient has 10 columns for patient index, status (BC versus HD, MBC versus NMBC, PD versus PR/SD, or high versus low baseline expression), and expression levels or relative intensities of the 8 protein markers.
5.	Put the data files to default path. Click Run on RStudio.
