{"text_format":"3","workbook":"xls","n_sheets":1,"sheets":["Sheet1"],"not_rendered":{"external_links":1,"defined_names":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Sheet1","index":0,"n_rows":42,"n_cols":8,"rows_kept":42,"merged":[[2,0,35,0],[36,0,39,0]],"flags":["formulas_cached_only"]}
["Supplementary Data 1. List of identified CRISPR-Cas12f1 systems"]
[null,"name","Species/sourece","PAM used for GFP activation efficiency","DR","tracrRNA","sgRNA-scaffold","protein sequences"]
["undocumented CRISPR-Cas12f systems","Ob2Cas12f1","Oscillospiraceae bacterium ","5'-TTTC","GATGCATCTCACGCGTGTCCGTGGCTTGAAGG","CTGGGACTTCACCCAAAATCGAGACAGTGGCCGTCAGCCTTCCCATCGGGAAGCGGGCAATACACTCGAAAAGGTTAAGATGCACATAGTAATCCGTGCATGAGCCAC","CTGGGACTTCACCCAAAATCGAGACAGTGGCCGTCAGCCTTCCCATCGGGAAGCGGGCAATACACTCGAAAAGGTTAAGATGCACATAGTAATCCGTGCATGAGCCACGAAAGTGGCTTGAAGG","MSKGSLAKVMKYELRYLDGAGSFEQMQERLWVLQRQTREILNRSTQISFHWDYTSREHFEQTGQYLDVFSETGYKRLDGYIYSRVKDSCGDMASGNINATLQKAWNKYGTSKLDVLRGQMSLPSYKKDQPLVIEKHNIRLSMDGQQALAEITLFSNKFKKENSLSSNVRFAFQLHDGTQRRILNSVLSGEYGLGQCQLVYDRPKWFLLLTYTFTPQNRQLDPDRILGVDLGECYALCASVFGEYGSLRIEGGEVTAYAKKLEARKRSLQKQAAVCGEGRKGHGTKTRVADAYQMQDRIANFRDTVNHRYSKALIDYALKNQCGTIQMEDLSGIRQDTGFPKFLQHWTYYDLQSKIENKAKEHDIRIVKINPRYTSQRCSKCGAIDSGSRTSQARFCCTKCGFTANADYNASQNISIKGIDLLIEKELGAKAE*"]
[null,"Ob3Cas12f1","Oscillospiraceae bacterium","5'-TTTC","GTTGCAACCCGCTCGCTGGTGTGACTTGAAGG","AAGGGACGACTTCCCGTCCCAAAATCGAGATAGTGGTCCTGATTCTTTGATTTCAAAGCGGACAATACACTCGATAAGGTTAAGATGCACATAGGAATCCGTGCATGGGTCACA","AAGGGACGACTTCCCGTCCCAAAATCGAGATAGTGGTCCTGATTCTTTGATTTCAAAGCGGACAATACACTCGATAAGGTTAAGATGCACATAGGAATCCGTGCATGGGTCACAGAAATGTGACTTGAAGG","MGKGEISKVMKYELRYLDGSGSFEEMQQRVWALQRKTREIQNRTVQIAFHWDYINREHFIQTGNNLNVLQETGYKRLDGYIYDRLKGQSAEMSGANLNATIQTAWKKYNSAKPKVLSGTMSVPSFKRDQPLIINSNCVKFSRSESECLAELTLFSREYKKEHDLSSNVRFAIRLHDSTQRSILERVLSGAYRKGQCQLVYQRPKWFLFLTYSFFPMQHDLDPEKYLGVDLGECCALYASSVGEYGSLKLEGGEITAFAKQLEARKRSMQKQAAYCGEGRIGHGTKTRVADVYKMENRIANFRDTVNHRYSKALIDYAVKHQYGTIQMEDLSGIKNDTGFPKFLRHWTYFDLQEKIDAKAREHGIHVVKVNPQYTSQRCSKCGSIDSRNRKSQKEFCCLNCGYKVNADFNASQNLSIKGIDVIIQKYIGAKSKQTENNG*"]
[null,"OsCas12f1","Oscillibacter sp.","5'-TTTC","GTTGCAACCCGCGTATGGGCGCGGCTTGAAGG","AGGGACGACTTCCCGTCCCAAAATCGAGACAGTAGCCGTAAAACTTTGAGTTTCAGAGTGGGCGACACACTCGAAAAGGTTAAGATATGCACATAGTAATCCGTGCATGAGCCGC","AGGGACGACTTCCCGTCCCAAAATCGAGACAGTAGCCGTAAAACTTTGAGTTTCAGAGTGGGCGACACACTCGAAAAGGTTAAGATATGCACATAGTAATCCGTGCATGAGCCGCGAAAGCGGCTTGAAGG","MGKGVLAKVMKYELRYLDGCGDFSNMQEQVWALQRQTREILNRSIQIAFQWDCANSEHHRKTGEYLDLKTETGYKRLDGHIYNCLKGQYEDMATSNLNATIQKAWKKYNSSKKEILRGSMSIPSYKMNQPLTLDKNTVKLSEGERNPIVTLTLFSDKFKRAQGVSNVKFSMPLHDGTQRAIFANLMNGTYQLGECQLVYKRPKWFLFVTYKFPPVEHPLDPDKILGVDMGEACALYASTFGEHGYLKIDGGEITKYAKKMEARIRSMQKQAAHCGEGRIGHGTKTRVSVVYQAKDKVARFRDTINHRYSKALIDYALKNQCGTIQMEDLTGIKEDTGFPKFLRHWTYYDLQSKIEAKAAEHGIQVVKINPRHTSQRCSRCGHIDKANRTSQADFCCTKCGFSANADFNASQNISIRNIDKIIAKAIGANRKQT*"]
[null,"Cb1Cas12f1","Clostridia bacterium","5'-TTTC","GTTGCAACACGCGCGAAGGTGCGGCTTGAAGG","GATTCAGGGGCGACTTCCCGCCCTGAAATCGAGAAAGTGGTCGTAAGCCGGAAGCATTTCCGCAGACAATACACTCGAAAAGGTTAAGATATGCACATAGTAATCCGTGCATGGGTCGCATT","GATTCAGGGGCGACTTCCCGCCCTGAAATCGAGAAAGTGGTCGTAAGCCGGAAGCATTTCCGCAGACAATACACTCGAAAAGGTTAAGATATGCACATAGTAATCCGTGCATGGGTCGCATTGAAAGGTGCGGCTTGAAGG","MAKGTVTKVMKYELRYLSGFSDFHAMQQAVWGLQRQSREILNKTIQMAFHWDYISRENFNANGVYLDVKAETGYKTYDGYIYNSLKSAYADMAAANLNAAIQKAWKKYKDAKMEVLRGTMSTPSYRSDQPVLINKNCVKLFDGGVRLTLFSDRFKRENNLNGNLEFAVQLHDGTQRSIFANLLNGTYALGQCQLVYDKRKWFLLVTYIFTPEKHELDPEKILGVDLGQTYALYASSVCARGTFRIEGGEAAECAHRLEQRKRSLQQQARFCGEGRVGHGTKTRVAAVYSAGDKIASYRDSINHRYSKALVEYAVKNGYGTIQMEDLTGIQNDLDHPKRLQHWTYYDLQTKIENKAKEHGVGVVKVNPRYTSQRCSRCGHIERENRPTQKVFCCKACGFEGNADYNASQNLSMRNIDKIIEKELSAKGE*"]
[null,"Cb2Cas12f1","Clostridia bacterium","5'-TTTC","GTTGCAACACGCGCGTAAGGATGACTTGAAGG","AATTTAGGACTTCCCTGAAATCGAGAAAGTGGCCGTAAGACGCAGTTCCTTGCGCCGGCAATACACTCGAAAAGGTTAAGATGCACATAGTAATCCGTGCATGGGTCAT","AATTTAGGACTTCCCTGAAATCGAGAAAGTGGCCGTAAGACGCAGTTCCTTGCGCCGGCAATACACTCGAAAAGGTTAAGATGCACATAGTAATCCGTGCATGGGTCATGAAAATGACTTGAAGG","MCALTKIMKYELRYLDGFPDFSAMQNAVWPLQRQTREILNRTIQEAYRWDYFSATKKKETGEYPDLQKETGYKRLDGYIYHVLSPDYPDFSSSGVNATIQKAWKKYKSSKADVWKGEMSLPSYKSDQPIVLHAKQIKLSGDTRAAAATLSLFSNKFKKEHEISGNVQFAITLHDNTQRTIYQKLRNGEYKLSESQLVYDKKKWFLYLAYSFNPAEHALDPEKILGVDMGEKFALYASSFGEYGHFKIEGSEVTEYAKALERRRRSLQQQARYCGEGRIGHGTKTRVGVVYREEDRIANFRSTINHRYSKALIEYAVKNGYGTIQMENLTGIKENLQFPRRLQHWTYYDLQSKIEAKAKEHGIAVVKVNPKHTSQRCSRCGHIAAENRPKQEVFQCVKCGYACNADFNASQNISIKDIEKLIQETIGANPK*"]
[null,"Cb5Cas12f1","Clostridiales bacterium","5'-GTTC","GTTGCAACTCGCACGTTGGCACTGGTTGAAGG","AATCGAGATAGCAGCCATTTGAAGACGGTCTTGCACTCGAAAAGGTCAAGATGCACACAATAATCCGTGCATGGTCAGTGT","AATCGAGATAGCAGCCATTTGAAGACGGTCTTGCACTCGAAAAGGTCAAGATGCACACAATAATCCGTGCATGGTCAGTGTGAAAACACTGGTTGAAGG","MAKKGNSQKKQIVKVMKYELKYEKGCADFNEMQNELWKLQRQTREVMNRTIQLCYHWSYVQAEYCKQHGCARRDVKPCDVYETNATSLDGYIYQLLKVEYPDFFMKNLNATLRKAHQKYDALLFDIQEGNSSIPSFKKDQPLIFEKKAICISKCLPDKRQITLSCFSDSYIDAHPTLDKITFTVRARSASEKSIFDHIISGKYALGTSQLVYEKKKWFFLLSYKFTPESVDVNPEKVLGVDLGVVNALCAGSVENPHDSLFIKGTEAIEQIRRLEARKRDLQKQARYPGDGRIGHGTKTRVSPVYQTRDAIARMQDTLNHRWSRALIDFACKKGYGTIQMEDLSGIKAMESEKPYLKHWTYFDLQSKIIYKAEEKGIRVVKVNPKCTSRRCSACGYISKENRKNQAEFLCVNCGYHHNADYNAAQNLSIPQIDRLIEKQLKEQESEESEAGTNPK*"]
[null,"Ob1Cas12f1","Oscillospiraceae bacterium","5'-GTTC","GTTGCAATTTGTATACGAGTGTGACTTGAAGG","TATTTAGGGCGACTTCACGTCCTCAAATCGAGAAAGTGAGCGTAAGACTTGGCTTCTGTCAAGCGGTTAATACACTCGAGAAGGTTAATATGCACATAGTAATCCGTGCATGAGTCACT","TATTTAGGGCGACTTCACGTCCTCAAATCGAGAAAGTGAGCGTAAGACTTGGCTTCTGTCAAGCGGTTAATACACTCGAGAAGGTTAATATGCACATAGTAATCCGTGCATGAGTCACTGAAAGTGACTTGAAGG","MAEKTIVKVMKFELRYIDGAGEFSEMQKHLWELQKQTREVLNKTIQMGYALECKRFAHHDKTGQWLDDKELTGSKYKAVADYINAELKEDYNIFYSDCRNSTVRKAYKKFKDAKNKIFSGEMSLPSYRSNQPIIIHNRNVIIRGNAESALVGLKVFSDGFKALHGFPAAVNFKLCVKDGTQRAIIENVISEIYKISESQLIYDNKKWFLILAYRFTQKKNDLNPDKILGVDLGVKFAVYASSIGEYGSFRIKGGEVTEFIKRLEKRKKSLQNQATVCGDGRIGHGTKTRVADVYKARDKISNFQDTINHRYSRAIVDYARKNGYGTIQLEKLDNSIEKKGDYSPVLVHWTYYDLRTKMEYKAAEYGIKVIAVEPKYTSQRCSKCGYISSENRKTQESFECIKCGYKCNADFNASQNLSVRDIDRIIDEYLGANPELT*"]
[null,"EsCas12f1","Eubacterium siraeum","5'-TTTC","GTTGCAATCTACATGCACGGGTATAGTGCAAG","CCGGGCGGCTTGGCGTCCGTAAATCGAGAAAGTACATTGTTAATATAGTGGATACACTCGATAAGGTTAACGCATACGATATTAATCCCGTATGCCGTCTATATTT","CCGGGCGGCTTGGCGTCCGTAAATCGAGAAAGTACATTGTTAATATAGTGGATACACTCGATAAGGTTAACGCATACGATATTAATCCCGTATGCCGTCTATATTTGAAAGGGTATAGTGCAAG","MVCNKVVKIALICDQIDKDGKDVNYNDIYKLLWDLQKQTREAKNKVIRLCWEWSGYSSEYFKTHEEYPKDKEILGISLRSYLYNRIKGDYNLYSGNLSQSAKIAYIEYKNSLTDVLRGDKSIINYRENQPLDIKNKAIQLLYENDNFFVRVALINKDKRKELNFKDCSVRFKLLVKDDSTRTILERCFDEVYTITASKIMYNKKKKQWYINLGYKFTKEIDKTLDKDRILGVDLGVINPLVASVYGSYDRLIIGGGEIDKFRKRVEANKVQMLKQGKYCGDGRIGHGVNTRNKPAYNIEDKISRFRDTVNHKYSKAVVDYAVKNNCGTIQMEDLKGITQNKNERYLKNWTYFDLQTKIEYKAKALGIEVKYKNPKYTSQRCSKCGHIAEENRPEQKTFKCVKCGFKVNADYNASQNLAIKDIDKIIEQYYNKG*"]
[null,"Pt1Cas12f1","Parageobacillus thermoglucosidasius","5'-TTTA","GTTTCTGAAGAAACTATGTATGATGTGAAG","AGTCGAGAAGTGCCGTAATAAGCATCTAAAAATGCCTAACGGTAACACTCGATAAGGTAGTCCTGCTAGGCAGGCTGAAACCCTAGCCACAAAATCCGGCTAGGCATCATAC","AGTCGAGAAGTGCCGTAATAAGCATCTAAAAATGCCTAACGGTAACACTCGATAAGGTAGTCCTGCTAGGCAGGCTGAAACCCTAGCCACAAAATCCGGCTAGGCATCATACGAAAATGTATGATGTGA","MKYTKVMRYQIIKPLNAEWDELGMVLRDIQKETRAALNKTIQLCWEYQGFSADYKQIHGQYPKPKDVLGYTSMHGYAYDRLKNEFSKIASSNLSQTIKRAVDKWNSDLKEILRGDRSIPNFRKDCPIDIVKQSTKIQKCNDGYVLSLGLINREYKNELGRKNGVFDVLIKANDKTQQTILERIINGDYTYTASQIINHKNKWFINLTYQFETKETALDPNNVMGVDLGIVYPVYIAFNNSLHRYHIKGGEIERFRRQVEKRKRELLNQGKYCGDGRKGHGYATRTKSIESISDKIARFRDTCNHKYSRFIVDMALKHNCGIIQMEDLTGISKESTFLKNWTYYDLQQKIEYKAREAGIQVIKIEPQYTSQRCSKCGYIDKENRQEQATFKCIECGFKTNADYNAARNIAIPNIDKIIRKTLKMQ*"]
[null,"RhgCas12f1","Ruminiclostridium hungatei ","5'-TTTA","ATTGCAACTGGTGCATGTGTACAAGATGTGAG","TAAATCGAGAAGTGGCATAAATCCATACTTGTGTGGTTGCAAAACACTCGATAAGGTAAAAACGGTTAGCACCGTTTGAAATTCCGAGTATAAAAGACCGCTCGGACGTCTT","TAAATCGAGAAGTGGCATAAATCCATACTTGTGTGGTTGCAAAACACTCGATAAGGTAAAAACGGTTAGCACCGTTTGAAATTCCGAGTATAAAAGACCGCTCGGACGTCTTGAAAAAGATGTGAG","MATKVMRYQIIKPIDCNWDLFGKVLRDIQYDTRQIMNRTIQYCWEWQGYSSDYKIAKGEYPKTRETFGYSDMRGYAYDKLKSIYQRLNTANLTTSITRAVQRWKTDTKDVIRGDKSIACFRADVPIDLHNKSMNIEKSDDGYIVALSLASNIYKKELDRNSGQFSVLINEGNKSNRDVLDRCIAGQYKISASQILREKNKWFLNLSYSFEISKPDKSRDNILGIDVGIVHPVYMAVYNSPARRSISGGEIDNFRKQVQKRIKELQLQGKQCGEGRIGHGIKTRVKPIEFAKDKVANFRNTINHKYSKAIVEFAIKNGCGIIQMEDLKGINTDNVFLKNWTYYDLQQKVKYKAELEGIEVKLIDPQYTSQRCCKCGYIHRDNRPEQAKFKCIDCGFEVNADYNASLNIATPDIDKIILEFLKCET*"]
[null,"Bc1Cas12f1","Bacillus cereus ","5'-TTCA","GTTTAACACTAACATAAGATGTATTTAAAT","AAATGGAGAAGTAGCACATAAGAAATTTACCAAGTGCCAACACTCCGTAAGGTAGTATCAAATGTAAATAAACATTGATGCGTGGGCACTTTCATGCTCTGAAGGGTGTAACACAAAAACCGTTACACAATACATC","AAATGGAGAAGTAGCACATAAGAAATTTACCAAGTGCCAACACTCCGTAAGGTAGTATCAAATGTAAATAAACATTGATGCGTGGGCACTTTCATGCTCTGAAGGGTGTAACACAAAAACCGTTACACAATACATC  GAAA  GATGTATTTAAAT","MGVTIKIMKYQILCPMNVDWTIFEKHLRNLTYQVRTISNRTIQQLWEFDALSFDYFKERGTYPTVQDLYGCTQKKIDGYIYHTLQSKYPDIHKGNMSTTLQKIIKTWKSRRNEIRKGEMSIPSFRNRIPIDLHNNSVDIIKEKNGDYIAGISLFSRDFHKENGDVPKGKIFVKLGTQKQKSMKVILDRLINQTYSKGACMIHKYKNKWYLSITYKFNAIKENKFDKELIMGIDMGGINTVYFAFNEGFIRSNIKSDEIKMFNERIRQRRINLLKQSKYCSNSRTGKGRTKRLQPIDVLSNKIAKFRNSTNHKYANYIVKQCLKHNCGRIQMELLKGISKNDKVLKDWTYFDLQEKIKNQAEIYGIEVIKVVPAYTSQRCSQCGYICKENRCTQAMFECKQCGYKTHADYNAAKNISTYDIENIINKQLAVQSKLHSKKCMEEYIEELGYLD*"]
[null,"BfCas12f1","Bacillus fungorum","5'-TTCA","AGTTTAAACCAAACAATAGATGTATTGAAAT","TAAATGGAGAAGTGACACACGGTAAATGTACCAAGTGTAAACGCTCCATAAGGTAGTATCGAATGTTTAAAAACATTGATACGTAGGCGTTATGAATGCCTTGAAGGGTGTAACACAAAGACCGTTGCACAATACAT","TAAATGGAGAAGTGACACACGGTAAATGTACCAAGTGTAAACGCTCCATAAGGTAGTATCGAATGTTTAAAAACATTGATACGTAGGCGTTATGAATGCCTTGAAGGGTGTAACACAAAGACCGTTGCACAATACATGAAAGATGTATTGAAAT","MSTVVKVMKYQIICPVNIEWKAFETYLRTLSYQVRTIGNRTIQKLWDFDNQSLNHFRENGVYPSAQQLYGCTQKTISGYIYDQLKEEYQDMNKANMSTTLQKTIKTWNSRKKEIRSGEMSIPSFRNNLPIDIHGNSIQITKEKSGDYIASLSLFSSNFIIENNLPNGKIQVKLSTRKQNSMKVILDRIIENTYAKGACMLHKHKNKWYLSIIYKPTVKEEHKFEEDLVMGIDMGKINVLYFAFNKGWIRGAISGEEIEAFRKKIEHRRISLLRQGKYCSGNRVGKGREKRIKPIDVLNNKIAKFRNATNHKYANYIVQQCLKYNCGTIQLENLQGISKEQTFLKNWTYFDLQEKIKQQAHQYGMKVVTIDPSYTSKRCSECGYIHKNNRKSQSTFECQQCNLKVHADYNAAKNISIYNIEKVIQKQLKLQEKLNSKKFTEQYIEQVENIN*"]
[null,"BtCas12f1","Bacillus thuringiensis","5'-TTCA","GTTAAAACATAACAATAGATGTATTGAAAT","AAATGGAGAAGTGATACACGGTAAATTTACCAAGTGTCAACGCACCGTAAGGTAGTATCGAATGTTAGAAAACATTGATACATAGGCATTCTGAATGCTTTGAAGGATGTAACACAAAGACCGTTACATAATACATT","AAATGGAGAAGTGATACACGGTAAATTTACCAAGTGTCAACGCACCGTAAGGTAGTATCGAATGTTAGAAAACATTGATACATAGGCATTCTGAATGCTTTGAAGGATGTAACACAAAGACCGTTACATAATACATTGAAAGATGTATTGAAAT","MSIAVKVMKYQIVCPVNIEWKTFEIYLRTLSYHFRTIGNRTIQKLWEYDNQSLKHFKDTGQYPSAQQLYGCTQKTISGYIYDQLKEEYQDINKANMSTTLQKTIKTWNSRKKEIWSGEMSIPSFRNNLPIDIHGNSIQIIKEKSGDYIASVSLFSSKFIKENDLPNGKILVKLSTRKQNSMKVILDRIIDSTYAKGACMLHKHKKKWYLSITYKSNIKEELKFDEDLIMGIDMGKINVLYFAFNKGLVRGAISGEEIEAFRKKIEHRRISLLRQGKYCSGNRIGKGRKKRIKPIEVLNDKIAKFRTATNHKYANYIVQQCLKYNCGTIQLEDLQGISKEQTFLKNWTYFDLQEKIKNQANQYGIKVVKIDPSYTSQRCSECGYIHKNNRQNQSTFECQQCSFKVHADYNAAKNISVYNIEKVIQRQLKLQEKLNLTKYKEQYIEQMENIN*"]
[null,"HsCas12f1","Hydrothermal sediment microbial communities from Guaymas Basin, California, USA","5'-TTTC","GTCACACCCTGTGCGGGTGTGTGGGTTGAAAC","GTGGTGGTTCGCGCAATGGGGCGAGTTCACGTCCTTATGTTGAGAAGTGCCTGTAATTCAATGAATTATCATTGTTTGTGTAACGCTCAATAAGCCTGCACACAATACCGCAC","GTGGTGGTTCGCGCAATGGGGCGAGTTCACGTCCTTATGTTGAGAAGTGCCTGTAATTCAATGAATTATCATTGTTTGTGTAACGCTCAATAAGCCTGCACACAATACCGCAC  GAAA GTGTGGGTTGAAAC","MRALENQKPLKSIKKPVCKISRTLSVPIQRPCGYVWNDFGHLLCIIRNDVAQAYNMAMSESYLYFSERENYKREHGKYPKVEQLAKRNVYKKLTENFPHIGTGILATIANKVESKLKKEYVEVMLKGTKSVSNYKKGTPIPIRAQGWKERTFKRKRKDKMTFHLLSKKAEQSKSLDFLKDEKGKIPCSFTVRIALKKLNNSQRAVYNRIWAGEYKAGAIDILQRKGKWFINISYHMLETKRLEKQLDKNVIVGVDLGIVNGVVCAVSNDAYDRLVLRKDIEGFRKQIWKRKHLAWKSTRRGGKGRKYYLRMSDSLKKKEHNFRNTLYHDWTRKVIDYALKHGAKVIQIEDLSGLVEAKKKMKKGVLKNWVISDFVEKLTYKAEEYGIEIVKVNPRYTSQRCHKCGHIEKDNRKEQSKFVCLKCGHSCNADFNAAKNIATKNIADIISASLPQT*"]
[null,"MsCas12f1","Marine sediment microbial communities from Aarhus Bay station M5, Denmark","5'-TTTC","GGTGTAGGCGACCTTTTTTTGCGGTGTACTTCGAGG","GTTCTTTGAAATAAAGATATAGCTGCCGGTAAAACGATAGCCCACGGGCAATTGCGTGCGGCAGTTTAGGCCGACTCGAACGGCCTGAAGGTTGAGGTAAAG","GTTCTTTGAAATAAAGATATAGCTGCCGGTAAAACGATAGCCCACGGGCAATTGCGTGCGGCAGTTTAGGCCGACTCGAACGGCCTGAAGGTTGAGGTAAAG ACTTCGAGG","MTDEQARLQKVATFQIVKPVNMDWREFRKLLRDVRYRLWRLGNMAVSEAYLTFHKKYRMGQAQSDGAHKLSVLDKRLRQALIDEGVRVEELSRYSRKGAVSGYICGAFEKTKLSAIKSKSKWRDIINGRASLPVFRRDLAIPINCSDCQPRMIERTEAGEYQVDLRICLQDKELAPNGYPRVLLSTAKISDGQRAVLERLVSNKTNSLPGYRHRFFEIKEKRGKWFLSVSYDFPRAEAGKLHQDIIVGVDLGWSVPLYAALNKGYARIGWKKLEPLAKRIRHLQKQVKGRRLSMQRGGQADLAGPTARMGHGRRRNLQAIEKLEGKINDAYTTLNHQLSHCIIEFAKNNGAGVIQIEDLRGLADELRATFIGQNWRYHQLQEFIKYKAEEAGIKVVPPVNPFYTSRRCSVCGYLHKDFTFEYRQVNRKNGMSVMFECPECSKKAKEEGKEYKALNADYNAARNLATANIEEKIRLQCKEQGIEYTELPKS*"]
[null,"ScCas12f1","Sediment core sample microbial community from Chocolate Pots hot springs, Yellowstone National Park, Wyoming, USA","5'-TTTG","GCCGCATCTCGCACGCGTACGTGGATTGAAAC","ATAAATTTCGTCCCGCGGTGATGGGTATAGCCTTTGGGCAGAGTCCCAAAATCGCGGATAAGACGCGGTTGCTTTCAACAGCCGAAAATCGAGCTAC","ATAAATTTCGTCCCGCGGTGATGGGTATAGCCTTTGGGCAGAGTCCCAAAATCGCGGATAAGACGCGGTTGCTTTCAACAGCCGAAAATCGAGCTAC  GAAA  GTGGATTGAAAC","MKDYIRKTLSLRILRPYYGEEIEKEIAAAKKKSQAEGGDGALDNKFWDRLKAEHPEIISSREFYDLLDAIQRETTLYYNRAISKLYHSLIVEREQVSTAKALSAGPYHEFREKFNAYISLGLREKIQSNFRRKELARYQVALPTAKSDTFPIPIYKGFDKNGKGGFKVREIENGDFVIDLPLMAYHRVGGKAGREYIELDRPPAVLNVPVILSTSRRRANKTWFRDEGTDAEIRRVMAGEYKVSWVEILQRKRFGKPYGGWYVNFTIKYQPRDYGLDPKVKGGIDIGLSSPLVCAVTNSLARLTIRDNDLVAFNRKAMARRRTLLRQNRYKRSGHGSANKLKPIEALTEKNELYRKAIMRRWAREAADFFRQHRAATVNMEDLTGIKDREDYFSQMLRCYWNYSQLQTMLENKLKEYGIAVKYIEPKDTSKTCHSCGHVNEYFDFNYRSAHKFPMFKCEKCGVECGADYNAARNIAQA*"]
[null,"Un2Cas12f1","Uncultured bacterium","5'-TTTG","GTTGCAGAACCCGAATAGACGAATGAAGGAATGCAAC","AATGTTATTCCATAATAACATTTGATGCACACGATTCCTCCCTACAGTAGTTAGGTATAGCCGAAAGGTAGAGACTAAATCTGTAGTTGGAGTGGGCCGCTTGCATCGGCCTAAAGTTGAGAAGTGTCAGACTCTGATAACCCTCAACGACGATATTCTTTATTTCG","AATGTTATTCCATAATAACATTTGATGCACACGATTCCTCCCTACAGTAGTTAGGTATAGCCGAAAGGTAGAGACTAAATCTGTAGTTGGAGTGGGCCGCTTGCATCGGCCTAAAGTTGAGAAGTGTCAGACTCTGATAACCCTCAACGACGATATTCTTTATTTCG  GAAA  CGAATGAAGGAATGCAAC","MEVQKTVMKTLSLRILRPLYSQEIEKEIKEEKERRKQAGGTGELDGGFYKKLEKKHSEMFSFDRLNLLLNQLQREIAKVYNHAISELYIATIAQGNKSNKHYISSIVYNRAYGYFYNAYIALGICSKVEANFRSNELLTQQSALPTAKSDNFPIVLHKQKGAEGEDGGFRISTEGSDLIFEIPIPFYEYNGENRKEPYKWVKKGGQKPVLKLILSTFRRQRNKGWAKDEGTDAEIRKVTEGKYQVSQIEINRGKKLGEHQKWFANFSIEQPIYERKPNRSIVGGLDVGIRSPLVCAINNSFSRYSVDSNDVFKFSKQVFAFRRRLLSKNSLKRKGHGAAHKLEPITEMTEKNDKFRKKIIERWAKEVTNFFVKNQVGIVQIEDLSTMKDREDHFFNQYLRGFWPYYQMQTLIENKLKEYGIEVKRVQAKYTSQLCSNPNCRYWNNYFNFEYRKVNKFPKFKCEKCNLEISADYNAARNLSTPDIEKFVAKATKGINLPEK*"]
[null,"CiCas12f1","Clostridium ihumii","5'-GGAG","TTAATGATTAACATAGGTTATATTTAAAT","AAAGGGTGATTTACCATCCTAAGTAGAGGAAACTCTTACAACCGCTCCAGTTGAAATTTGCTGGTAAAAAGCTAGTATAAAAAATGCTATAAGCATGGTGGGTACTATGATATAACCAAT","AAAGGGTGATTTACCATCCTAAGTAGAGGAAACTCTTACAACCGCTCCAGTTGAAATTTGCTGGTAAAAAGCTAGTATAAAAAATGCTATAAGCATGGTGGGTACTATGATATAACCAATGAAAATAGGTTATATTTAAAT","MKTTEKNVLMTKCIKVTLNRCVNYNMKEIMNIIREMQYLSSKAYNLATNYLYIWDTNSMNFKNLYEEKIVDKDLLGKSKSAWIENRMNEIMKGFLTNNVAQARQDVINKYNKSKKDGLFIGKVTLPSYKMNGKVVIHNKAYRFSKNEGYFVEIGLFNKEKKEELNCDWIKFKLDKIDSNKKATIYKILNGDYKQGSAQLHINKKGKIEFIISYSFERENSIKLDKNRTLGIDIGIVNIAAMAIWDNNKQEWELTRYSHNLISGNEAIALRQKYYKLGLRNKELEKNINRELHELEEKEYRGLSTNIISGHNLTYKRIMLNSKRIRLSQSCKWCGNSKVGHGRRVRCKQVDKIGNKIERFKDTFNHKYSRYIVDFAVKNNCGIIQMENLKNFNPSEKFLKDWPYFDLQTKIEYKAKEYGIEVIKVNPKYTSKRCSRCGCINELNRDCKKNQSKFKCVNDECNNYENADINAAKNIALPYIDKIIEQCLETNKVV*"]
[null,"CpCas12f1","Clostridium paraputrificum","5'-GGAG","TTGTTTAATATTAACAAAGGTTGTATTTAAAT","GTATCTATAACATAAAACTAAGATTATTAGTAAGTTTATAGAATAATATCTCGATAATGCTTCAAGTATTAATTCACTTGGTAAAGGTTGTGTAGGGGAGTGGCTTTAAGTCAGAGTTCTACACCGATACAACT","GTATCTATAACATAAAACTAAGATTATTAGTAAGTTTATAGAATAATATCTCGATAATGCTTCAAGTATTAATTCACTTGGTAAAGGTTGTGTAGGGGAGTGGCTTTAAGTCAGAGTTCTACACCGATACAACT GAAA  GTTGTATTTAAAT","MKLNKCIKVTLVKCLNYDYKEIKQIIRDFNYTACKASNKAMRMWFFHTQDMIDKKNKYKEFNQIQYEKDTYGKSYRNVIEGEMKKIMPLANTSNVGTLHQQLVQNDWSRLKKDILSCKANLPTYKLSTPYFIKNDNFKLRNHNGYFVDIAFFNKEGLKQYGYKAGHKFEFQIDKLDGNKKSTINKIINGEYKQGSAQLSISNKGKIELIISYSFEKEEVPVLDKNKILGIDLGITNVATMSVYDSMREQYDYFSWKTNVISGKELIAFRQKYYNLRRDMSIASKTAGQGRCGHGYKTKMKSVNKVRNKIANFADTYNHKISKYIIEFAIKNNCGVIQVEDLSGATADTHNKMLKDWSYYDLQQKIEYKAKEQGIEVIKVNPKYTSKRCSKCGCIHEDNRDCRNNQAKFECKVCGYNENADINASKNIAIPDIDNIIKGTEILHSKENKAS*"]
[null,"SvCas12f1","Sarcina ventriculi  ","5'-GGAG","GTTTAAGAATAACAATAGTTGTATTTAAAT","AAGGTGAGAAGTGCTGTAGGAGTCCCTTGCAGTAACTCTCTACCAAACGCTCCTGTATTAAATAGGTAAAAGCCTTGTGCAAAACATTAAAATGGGCGTGTACAAGAATACAAT","AAGGTGAGAAGTGCTGTAGGAGTCCCTTGCAGTAACTCTCTACCAAACGCTCCTGTATTAAATAGGTAAAAGCCTTGTGCAAAACATTAAAATGGGCGTGTACAAGAATACAATGAAAGTTGTATTTAAAT","MTTKCVQVAIEYSSNNILKEVDFYKELRDLQYNSYLACNRAISYMYENDMQNFIIKETDLPRSDDKKLYGKSFAAWIENRMNEYMPGALSNNVAQTRQFVVNRYKNDKKAGLLKGNVSLTTFKRTNPIIIHNNAYNIIETPKGLGAEIGFFNLPKQKELGIKRVNFLFPKLGSSEKSIIRRLLDKSYKQGAMQISYNQKKKKWMATISFSFNLEEIKTNENLVMGIDLGVSKVATLSIYDASKYEYIKMSFKDTCIDGTELMHYRQKLESRRKALSIASKWASDNNRGHGYKTKMEKANYMGRKYNNFRDTYNHKVSRYIVDVAIKYRVGLIQMEDLSGFSEQQQESLLKNWSYYDLQQKIKYKAEENGIRVYFINPKYTSQRCSKCGNIDKENRKTQESFSCTVCNYKDNADVNASKNIAIPDIEKIIEEQVKKQY*"]
[null,"AoCas12f1","Anaerobic oil degrading microbial communities from River Tyne estuarine sediment","5'-TCCA ","GTCGCAACCTATATGGATACGTGGATTGAAAT","AGGCTCAGGCGAATTTGCGTCTGTAGAGGGAGGCAGGGTGTAACACGATAGCCCGTATAGCACTCCCTAAAGGGTTAACCTTGGTCATTATGTTACCGACCAAGCAATACCACA","AGGCTCAGGCGAATTTGCGTCTGTAGAGGGAGGCAGGGTGTAACACGATAGCCCGTATAGCACTCCCTAAAGGGTTAACCTTGGTCATTATGTTACCGACCAAGCAATACCACA  GAAA   GTGGATTGAAAT","MITTRKLKLAIVSDNKNEAYSFIRDETRNQNRALNVAYSHLYFEYIAQEKLKHSDAEYQEHLAKYQELASKKYQEFLKVKEKAKSDETLQAKVDKAREAYNKAQEKVYKIEKDYSKKAREIYQQSVGLAKQTRIDKLLKNQFNLHYDTVDRVGGTAISHFTNDMKSGVLQGKRSLRNYKSSNPLMIRARSMKVYEENSDYYIKWIKDITFKIIISAGSKQRQNIGELKSVLVNIIEGNYKACDSSIGVDKDLILNLSIDIPITKENIFIPNRVVGVDLGLKIPAYVSVNDTPYIKRAIGNINDFLKVRTQLQSQRRRLQKALQSTNGGKGRNKKMQGLERLQAKEKNFVNTYNHFLSKNIVDFAVKNNAGMIHMEELKFDKVKHKSLLRNWSYYQLQTMIEYKAKREGIEVYYVDASYTSQTCSKCGNLEEGQRETQDTFVCKKCGYSVNADYNASQNIAKAKTIKEENQQ*"]
[null,"Bc2Cas12f1","Bacillus cereus","5'-TCCA ","GTTTGATATCAACTATATGGAATGTAAAT","CGATTTAGCGTCTAAAGGGTGAGATTGTAGCTCACCAAGGGTTAACTCTAGTCTTGTTATGTTACCGACTAGACATTCTATATTGATATCGAATGAACATTCCGTA","CGATTTAGCGTCTAAAGGGTGAGATTGTAGCTCACCAAGGGTTAACTCTAGTCTTGTTATGTTACCGACTAGACATTCTATATTGATATCGAATGAACATTCCGTAGAAATATGGAATGTAAAT","MILTRKIKLVIVSENREEGYNLIRTEIREQHKALNLAYNHLYFEHNAIQKLKQNDEDYKQKRNKLQELINKKYEEHQKAKNLEKKEALREAYNNKKQELYNFEKEYNEKARQTYQQVVGFTQQTRVRNLINRECNLMSDTKDGITSKVTQDYKNDCKAGLLIGKRSLRNYKKDNPLLVRGRSLKFYKEDGDYFIKWNKGTIFKCILHIRKKNVVELQSVLENVLLGAYKVCDSSIGFNNKDMILNLSLNIPDKETQGYIPGRVVGVDLGLKIPAYLSLSDKVYVRKGIGSIDDFLRVRTQMQKRRRRLQKSLAAVKGGKGREKKLKALDHLKGKEANFAKTYNHFLSTQIVTFAVKNQAGQINMEFLEFDKMKNKSLLRNWSYYQLQIMVEYKAKREGIIIKYVDAYLTSQTCSKCDHYEDGQREKQENFMCKNCGLEVNADYNASQNIAKSTSYISDSTESEYHKKKQQVLKEILGENDIMNEQLSLFNNCDDIA*"]
[null,"CdCas12f1","Clostridioides difficile ","5'-ATCA ","GTTGAAGAATAACATGAGATGTTTTTAAAT","ATGTGAGGTCATGTGATATAGGCACTCGCAAAGATAGTTGCTAAAGGTAGCAATTATCATCGTCCTAGTGAATTGCTAGGTAATAACA","ATGTGAGGTCATGTGATATAGGCACTCGCAAAGATAGTTGCTAAAGGTAGCAATTATCATCGTCCTAGTGAATTGCTAGGTAATAACAGAAATGTTATTAAAT","MISTRKIKVRCDDSTFYTFFRQEQREQNKALNIGIGIIHANAVLHNVDSGAEKKLKKSIEGLQGKIDKLNKDLEKEKITDKKKEEVLKAIETNKKILDGEKKVFKESEEYRKGIDELFKNTYLKSNTLDHVLDSMVNIQYKRTLSLVTQRIKKDYSNDFVGIITGQQSLRNYRNDNPLMISNQQLNFKYIDDTFYLDIMCGYRLEVVLGKRDNENVNELKSTLEKVISKEYKVCDSSMQFSKNNKDVILNLVIDIPQNSNVYKPVEGRILGVDLGVAVPIYMCLNDDTYKRKGLGDINNFLRVRQQMQTRRRKLQKDLTLTNGGKGRKKKTQLLDKLQENERNFVKTYSHALSKRVVEFAKSNKCEYINIEKLTKDGFDNIILRNWSYFELQKMIEYKAEREGITVRYVNPAYTSQKCSRCGEIDKENRQTQANFKCTKCGFELNADHNAAINIARSIEFV*"]
[null,"Cs1Cas12f1","Clostridium sporogenes","5'-ACCA ","GTTTTATCTTAACTATATGGAATGTAAAT","AGATAATAAGAACAGGGCGATTTAACGTCCTAAGGCTGAGGGATATTTCCACTCGGCAAGGGTTAATTTCGGATATTGTGTTACCATCCGAACATTCCATG","AGATAATAAGAACAGGGCGATTTAACGTCCTAAGGCTGAGGGATATTTCCACTCGGCAAGGGTTAATTTCGGATATTGTGTTACCATCCGAACATTCCATG  GAAA  TATGGAATGTAAAT","MNTVRKIKLTILGDTETRNKQYKWIKDEQYNQYRALNLSMTYMVTNLMLKNNESGLENRKEKDILKIENKIKKDEGSLKKELAKKKINEEKIENIKSNIEELKSEKEKLENELKNIKEYRSNIDEEFKKMYVDDLYNVLNKISFQHEDMKSLVTQRVKKDFNNDVKEIMRGDRSVRNYKRNFPILTRGRDLKFQYIEKSEDIEIKWIEGIKFKCILGKPSKSLELKHALHKVINKEYKVCDSSLQFDKNNNLILNLTLDIPQDNKYEKITNRVVGVDLGLKIPAYVALNDTKYIRKAIGSIDDFLKVRTQMQSRVRKLQKSLQVVRGGKGRNKKMKALERFREKERNFARNYNHFLSYNIVKFALDNKAEQINLELLEMKKTQNKSILRNWSYYQLQNFIEYKAERVGIKVKYIDPYHTSQTCSECGNYEEGQRVEQDTFVCKRCWHKMNADYNAARNIAMSYNYISKKEESEYYKNNKNMV*"]
[null,"RhCas12f1","Ruminiclostridium herbifermentans","5'-CCCA/TCCA ","GTTAAAAGCTAACTATAGTGGAATGTAAAT","ACGGTTGATTTAGCAACCGAAGTCTGAGGGCATGTAGAAAAAAGTATAGGTATATACCAACATACTTGCATTGCCACTCGGAAAGGGTTAACCTTGGTCATTGTGTTACCGACCAAGCATTCCA","ACGGTTGATTTAGCAACCGAAGTCTGAGGGCATGTAGAAAAAAGTATAGGTATATACCAACATACTTGCATTGCCACTCGGAAAGGGTTAACCTTGGTCATTGTGTTACCGACCAAGCATTCCA  GAAA  TGGAATGTAAAT","MITVRKLKILIDGESRNESYKFIRDSMYAQYLALNKAMSYLGTAYLSRDKEIFKEAIKSLNNSNPIFDNINFGKGIDTKSSVNQTVKKHIQADIKNGLAKGERSIRNYKRDYPLMTRGRDLKFFYCDTNSTKVKVKWVNGIIFDVMLGKEYNKNDLELRSFLNRVINKEYKISQSSICFDKHNRLILNLSVNITDNIPNEVVKGRIVGVDLGMKIPAYVTLNDSEYIGKPIGDINDFLKVRKQFKERKERLQKQLAINKGGRGITNKMQLMDAFTNKEKNFANTYNHGVSKAIINFAKKYKAEQINVEFLALAGSEKEILSSTIRYWSYYQLQQMIEYKANREGIAVKYVDPYLTSQTCCKCGNYEVGQRINQELFECKLCGNKMNADRNASFNIARSTKYISSKEESDFYKQLK*"]
[null,"Cb3Cas12f1","Clostridium botulinum","5'-TCCA ","GTTATAAATCTACTATGTAGTATGTGAAT","AGTCTATTAATAAATAGGTATGTAATAGCATATAAACCGAAGGGTGAGAGAATAGACTTTCATGTATTAGGATTTACCAACAATACACTATTACTACTCACTAAGGGTAAGCCCAGGTGTTAAGTTACCGCCTGGCATACTA","AGTCTATTAATAAATAGGTATGTAATAGCATATAAACCGAAGGGTGAGAGAATAGACTTTCATGTATTAGGATTTACCAACAATACACTATTACTACTCACTAAGGGTAAGCCCAGGTGTTAAGTTACCGCCTGGCATACTA  GAAA  TAGTATGTGAAT","MNTVRKIKIIINNENNELRKEQYKFIRDSQYAQYQGLNRCMGYLMSGFYVNNMDIKSEEFKTWQKGVTNSANFFQEISFGKGIDSKSSITQKVKKDFSIALKNGLAKGERNINNYKRIAPLMTRGRNLKFKYDDNELDILINWVNKIQFKCVLGEHKNSLELQHTLHKVINNEYKIGQSSLYFNKKNELILILTIDIPTAKSSYEPIKDRILGVDLGMAVPVYMSINDNSYIKKSLGSYSEFAKVRKQFKERRNRLYKQLEACKGGRGRKDKLKAMNQFKEKEKNFAKTYNHFLSKNIVEFALKNKCEFIHLEKIESKGLENSVLANWTYYDLQEKIIYKAKREGIGIKFVNSSYTSQTCSKCNYVDKENRKTQAKFICKNCGFKANADYNASQNISKSKEFIK*"]
[null,"Cb4Cas12f1","Clostridium baratii","5'-TCCA ","GTTATAAGTTAACTATAATTGATGTGAAT","ACGGTATATTCAAATGCCGAAGAATGAGAGATATTTGATTTAAAATAGCTAGGTTTAGGCCAACAGTTATAAAAATCTACTCGTTAAAGGTTAATCCAGATGTTATGTTACTGTCTGGCATCATATA","ACGGTATATTCAAATGCCGAAGAATGAGAGATATTTGATTTAAAATAGCTAGGTTTAGGCCAACAGTTATAAAAATCTACTCGTTAAAGGTTAATCCAGATGTTATGTTACTGTCTGGCATCATATAGAAATATATGATGTGAAT","MNIVKKIKLRIIDNDKELCKKQYLGFTEEQKKELIDKQYKFIRDSQYQQYLGFNRAMGFLMSGYYANNMDIKSDNFKEHQKKLTNSLYIFDDIKFGVGIDSKSLIVQRVKKDFSTALKNGLAKGERSVTNYKRTYPLLTRHRSIKFLYAENELDIYLDWVNKIRFRCELGNHKNSLELQHTLRKVITGEYKISDSSLEFNKKNELILNLNLNIPETKATFIKDRTLGVDLGMAIPAYVSLSDTPYIRKGFGSYEEFAKVRNQFKDRRKRLLKQLSLVAGGKGRAKKLHSMEFLKNKEKQFAKTYNHSLSKKIIDFALKNNCEYINLEDIKSTSLEDRVLGQWGYYQLQEQIEYKAKLVGIKVRKVKAAYTSQTCSECGNIDKENRKNQSTFKCTNEDCKLNKKGINADWNASINIARSKEFIK*"]
[null,"BsCas12f1","Blautia sp. M16 M6_ctg015","5'-TCCA ","GGTTTAATAGCACCATAATGGAATGTTAAT","TTGTATTGATGTTATATATAAATATATAGCAGTTACGGTAACTTAAAGTGCCGAAGGCTGAGGAGATGGATTAAATATATAAGGTTTTGACCAACTATATACCATCATCACTCGGTAAGGGTTAATCCTAACAATGTGTGACCGTTAGGCGTTCCAAA","TTGTATTGATGTTATATATAAATATATAGCAGTTACGGTAACTTAAAGTGCCGAAGGCTGAGGAGATGGATTAAATATATAAGGTTTTGACCAACTATATACCATCATCACTCGGTAAGGGTTAATCCTAACAATGTGTGACCGTTAGGCGTTCCAAA GAAA   TGGAATGTTAAT","MITVRKVKLIVNSEEAEEINRTYKFIRDSMYAQYQGLNRCMGYLLSGYYANGMDIKSDGFKNHMKTIKNSLNIFDDINFGIGIDSKSAITQKVKKDFSTSLKNGLAKGERGATNYKRNFPLMTRGRDVKISYLEDTNTFVIKWVNKIEFKVILGQKDNIELSHTLHKIINKEYTLGQCTFEFDKNNKLLLALNINIPDNLISKNKEIIPGRVLGVDLGVKVPAMICLNDNTFIKKSIGSYNEFFKVRSQFKARRERLYKQLESSNGGKGRKHKLKATMQFRDKEKNFARTYNHFLSKNIIEFAQKYTCETINLEELNKKGFDNNLLGKWGYYQLQSMIEYKAERVGIKVKYVDPAFTSQTCSKCGYVDEENRITQDKFECQKCGFTLNADHNAAINIARK*"]
[null,"Pt2Cas12f1","Parageobacillus thermoglucosidasius","5'-TCCA ","GTTATATAATGACTATGTAGTGTGTAAAC","AGGGTGAGGGTATAGATAAAACGCATAAGGTAGTATGCCAAATATGTGCTATAACCACTCGCTAAGCCGAAAAAACCTTAGTTTATGATGGCAACTAAGCACACTAT","AGGGTGAGGGTATAGATAAAACGCATAAGGTAGTATGCCAAATATGTGCTATAACCACTCGCTAAGCCGAAAAAACCTTAGTTTATGATGGCAACTAAGCACACTATGAAAGTAGTGTGTAAAC","MIAVKKLKLTIVEEEEKRKEQYKFIRDSQYAQYQGLNLAMGILTSAYLVSGRDIKSDLFKDSQKSLTNSNEIFNGINFGKGIDTKSSITQKVKKDFSTSLKNGLAKGERGFTNYKRDFPLMTRGRDLKFYEEDKEFYIKWVNKIVFKILIGRKDKNKVELIHTLNKVLNKEYKVSQSSLQFDKNNKLILNLTIDIPYKKVDEIVKDRVCGVDMGIAIPIYVALNDVSYVREGMGTIDEFMKQRLQFQSRRRRLQQQLKNVNGGKGRKDKLKGLESLREKEKSWVKTYNHALSKRVVEFAKKNKCEYIHLEKLTKDGFGDRLLRNWSYYELQEMIKYKADRVGIKVKHVNPAYTSQTCSECGHADKENRETQAKFKCLECGFEANADYNAARNIAKSDKFVK*"]
[null,"CrCas12f1","Cellulosilyticum ruminicola","5'-TCCA ","GTTATATATTAACTAAGTGATATGTAAAG","TTATGTCCAGATTTAGTGGGGCAAGAGTGAGGGCGTAGGTTAAAAGTGTAAGGCTAATAGCAACTACATTCTACGACACTCGCTAAACGGTAAAAACTCTAGCCTAATATTCTCAAGAGATTACGTGGCTAGACATATCAC","TTATGTCCAGATTTAGTGGGGCAAGAGTGAGGGCGTAGGTTAAAAGTGTAAGGCTAATAGCAACTACATTCTACGACACTCGCTAAACGGTAAAAACTCTAGCCTAATATTCTCAAGAGATTACGTGGCTAGACATATCACGAAAGTGATATGTAAAG","MIAVRKLKIMVLCDDESKKNEQYKFLRDSQYAQYLGLNRAMSFLAKEYLSGDKERFKEAKKKLTNTCECYQNINFGTGIDSKSQITQKVKKDLQADIKNGLARGERSIRNYRRTFPLITRGRDLKFSYNGDEIIIKWVNKIYFKVLIGRKDKNYLELMHTLEKIINGEYKVCTSSIQIDKKLILNLTLEIPDKVKKEFQENRVLGVDLGIKFPAYACVSDNTYVRRSFGSIDEFLKVRIQFDKRRKRIQQQLQNVKGGKGRKDKLQALDRMRDCERKWVRNYNHALSKRIIDFAFRNKCGIIHLEKLEKDGFKNKLLRNWSYYELQDMIGYKAEREGIVVKYVEPAYTSQTCSKCGYVDRENRPSQEHFLCKECGFEINADHNAAINIARSNKVIVDK*"]
[null,"ChCas12f1","Clostridium hiranonis strain DSM 13275","5'-TCCA ","ATTTGATTAGAACCATATTGGAATGTAAAT","TCTATAGGCGATTTAGCGTCTAAAGGTTGAGGGATAAGACAAAATGGTTAAGGTTTCGACCAACTAACTACTTATCCACTCGATAAACGGTAAAAACTCATACTAATATTCTTTATAGATAACGTGGTATGACATTCCAA","TCTATAGGCGATTTAGCGTCTAAAGGTTGAGGGATAAGACAAAATGGTTAAGGTTTCGACCAACTAACTACTTATCCACTCGATAAACGGTAAAAACTCATACTAATATTCTTTATAGATAACGTGGTATGACATTCCAA GAAA TTGGAATGTAAAT","MITVRKLKLTIINDDETKRNEQYKFIRDSQYAQYQGLNLAMSVLTNAYLSSNRDIKSDLFKETQKNLKNSSHIFDDITFGKGTDNKSLINQKVKKDFNSAIKNGLARGERNITNYKRTFPLMTRGTALKFSYKDDCSDEIIIKWVNKIVFKVVIGRKDKNYLELMHTLNKVINGEYKVGQSSIYFDKSNKLILNLTLYIPEKKDDDAINGRTLGVDLGIKYPAYVCLNDDTFIRQHIGESLELSKQREQFRNRRKRLQQQLKNVKGGKGREKKLAALDKVAVCERNFVKTYNHTISKRIIDFAKKNKCEFINLEQLTKDGFDNIILSNWSYYELQNMIKYKADREGIKVRYVNPAYTSQKCSKCGYIDKENRPTQEKFKCIKCGFELNADHNAAINISRLEE*"]
[null,"Cs2Cas12f1","Clostridium sp","5'-TCCA ","GTTTAATATAAACTATGTAGTATGTGAAT","ATAGTCGATTCAGCGACTAAAGGTTGAGGATATAGGATAAATAGCTAGGATAACCTAAAGCTATCTATAATCACTCAATAAGGGTTAACTCTAGATGTTGTGTTACCGTCTAGACATACATA","ATAGTCGATTCAGCGACTAAAGGTTGAGGATATAGGATAAATAGCTAGGATAACCTAAAGCTATCTATAATCACTCAATAAGGGTTAACTCTAGATGTTGTGTTACCGTCTAGACATACATA  GAAA  TAGTATGTGAAT","MITVRKLKLTIVGDEQTRKEQYKIIRDEQYQQYKALNLCMTLLNTHNILNSYNTGSENKLNSQIEKLDNKIEKNKIELKKGNLKESKIEKLNKSILELTKEKEKLQQEYLSASKYRSDIDEKLKDMYIKDMYTVVQSQVNFKSKDMMSLVTQRAKKDFSNALKNGMARGERSLINYKRDFPLMTRGERWLKFKYNEESDDIYIDWLHDIKFKVILGYKKNENSIELRHTLHKVINKEYKICDSSMQFDRNNNLILNLTLDIPNKESKGYVEGRTLGVDLGIKYPAYVCLSDDTYKRKSIGCAEDFIRVREQIRGRRYRLQKQLSMVKGGKGRDKKLRALDRVREAERNFVKTYNHMISKNIIKFAKEHNCEYIHLEKLTKDGFPDIILSKWSYYELQNMIEYKSDREGIKVRYIDPAYTSQTCSKCGHIDKENRINQEKFKCVKCGFELNADHNASINISRSNKYLK*"]
[null,"PhCas12f1","Peptacetobacter hiranonis strain ","5'-TCCA ","ATTTGATTAGTACTATAATGGAATGTAAAT","ATAATAAATATATATAGGCGATTTAGCGTCTAAAGATTGAGGTGTAGGAACTAACGGTTAAGGTTTATACCAACTAATTACCTATGCACTTGATAAACGGTAAAAACTTATACTAACATCCTTTATAGATAACGTGGTATGACATTCTA","ATAATAAATATATATAGGCGATTTAGCGTCTAAAGATTGAGGTGTAGGAACTAACGGTTAAGGTTTATACCAACTAATTACCTATGCACTTGATAAACGGTAAAAACTTATACTAACATCCTTTATAGATAACGTGGTATGACATTCTA   gaaa  TGGAATGTAAAT","MKTTRKLKLTIIGDEETRKEQYKIIREEQYQQYKALNLCMTLLNTHNILNSYNTGAENKLNAQIDSIDKKIEQAKKELEKKGLKESKVSKLKETIEFLENDREKLKDEYLNSSKFRSDIDEKMKEMYIKDMYTVVQNQVNFRARDMMSLVTQRARKDFKNSLKNGMAKGERSLTNYKRDFPLMTRGERWLKFEYDKDSDDILINWIHGIKFKVLLGYKKNENSIELRHTLHKVINKEYKICDSSMQFDRNNNLILNLTLDIPDKQNNNYIEKRTLGVDLGIKYPAYVCLNDDTYIRSHIGESLELLKQREQFKDRRKRLQQQLKNVKGGKGRNKKLSALNKLSDNERNFARTYNHMISKRIVEFAKKHRCEFINLEKLTKDGFDNNILSNWSYYELQNMIEYKAKREGIEVRYIDPAYTSQKCSRCGYIDKENRQTQEKFKCLKCEFEINADHNAAINIARALD*"]
[null,"OpbCas12f1","Opitutae bacterium","5'-CCCA ","CTTCCAATTTGCGCGTGGGCGTGAGTTGGGGG","TATGGGGCTGGATTGCGACTTCGGGAGCGCAAACAGACCCAGAAGATGCCTTCGGGCATCAACCGCCCTGCCAGGACGGGGCGCAATTCAC ","TATGGGGCTGGATTGCGACTTCGGGAGCGCAAACAGACCCAGAAGATGCCTTCGGGCATCAACCGCCCTGCCAGGACGGGGCGCAATTCAC GAAA GTGAGTTGGGGG","MSEQEAAQEGTKLLAKTLTFGLGNPMGFKSKGSVLVELTEDQRKAIYNGLRDASTVVARIINLLNSREYIRQIMKVPEELVAQFKPNYSLVKGPLKRLGIEEAEQVAGSVLSQTFALGVKPDFQGEHGKGLLLKGERQIPLHRTDGTHPIPQRATETRLFQVEKNFYVAMQVFAETWAKKQELPSGWLAFPIKVKPRDKTMAGQLLKTIGGEWKLKNSRLMRNPRTGGNRWLGQIVVAFAPEPFKKMTRSVVMGIDLGVNVPACLHISENGKPLPWAMMVGRGRDMLNTRNLIRSEIVHIIKALKSKDSPLDGKARAIYRDKLRDLRKRERRVMKMASQTVAARIADTAKRHGAGTWQMEDLSPDIKTDQPWLARNWAPGMLLDAVRWQARQCGAELVMVNPAYTSQRCARCGHIDPQNRPKQTDFKCMACGHEDNADKNAARNLSVVGIEKLIADFKAPNGAVQ*"]
["reported CRISPR-Cas12f systems","CnCas12f1","Clostridium novyi","5'-ACCT","GTTTTAGTTTAACTATGTGAAATGTAAAT","ACAGGGCGATTTAACGTCCTAAGGCTGAGAGAAGTTCCTTCTACTCGGCAAGGGTTAATCTCGATTGTTGTGTTACCGATCGAGCGTTTCACAGAAATGT","ACAGGGCGATTTAACGTCCTAAGGCTGAGAGAAGTTCCTTCTACTCGGCAAGGGTTAATCTCGATTGTTGTGTTACCGATCGAGCGTTTCACAGAAATGTGAAATGTAAAT","MITVRKIKLTIMGDKDTRNSQYKWIRDEQYNQYRALNMGMTYLAVNDILYMNESGLEIRTIKDLKDCEKDIDKNKKEIEKLTARLEKEQNKKNSSSEKLDEIKYKISLVENKIEDYKLKIVELNKILEETQKERMDIQKEFKEKYVDDLYQVLDKIPFKHLDNKSLVTQRIKADIKSDKSNGLLKGERSIRNYKRNFPLMTRGRDLKFKYDDNDDIEIKWMEGIKFKVILGNRIKNSLELRHTLHKVIEGKYKICDSSLQFDKNNNLILNLTLDIPIDIVNKKVSGRVVGVDLGLKIPAYCALNDVEYIKKSIGRIDDFLKVRTQMQSRRRRLQIAIQSAKGGKGRVNKLQALERFAEKEKNFAKTYNHFLSSNIVKFAVSNQAEQINMELLSLKETQNKSILRNWSYYQLQTMIEYKAQREGIKVKYIDPYHTSQTCSKCGNYEEGQRESQADFICKKCGYKVNADYNAARNIAMSNKYITKKEESKYYKIKESMV*"]
[null,"Un1Cas12f1","Uncultured bacterium","5'-TTTG",null,null,"accgcttcacttagagtgaaggtgggctgcttgcatcagcctaatgtcgagaagtgctttcttcggaaagtaaccctcgaaacaaagaaaggaatgcaac","MAKNTITKTLKLRIVRPYNSAEVEKIVADEKNNREKIALEKNKDKVKEACSKHLKVAAYCTTQVERNACLFCKARKLDDKFYQKLRGQFPDAVFWQEISEIFRQLQKQAAEIYNQSLIELYYEIFIKGKGIANASSVEHYLSDVCYTRAAELFKNAAIASGLRSKIKSNFRLKELKNMKSGLPTTKSDNFPIPLVKQKGGQYTGFEISNHNSDFIIKIPFGRWQVKKEIDKYRPWEKFDFEQVQKSPKPISLLLSTQRRKRNKGWSKDEGTEAEIKKVMNGDYQTSYIEVKRGSKIGEKSAWMLNLSIDVPKIDKGVDPSIIGGIDVGVKSPLVCAINNAFSRYSISDNDLFHFNKKMFARRRILLKKNRHKRAGHGAKNKLKPITILTEKSERFRKKLIERWACEIADFFIKNKVGTVQMENLESMKRKEDSYFNIRLRGFWPYAEMQNKIEFKLKQYGIEIRKVAPNNTSKTCSKCGHLNNYFNFEYRKKNKFPHFKCEKCNFKENADYNAALNISNPKLKSTKEEP"]
[null,"SpCas12f1","Syntrophomonas palmitatica","5'-GTTC","GTCGCATCTTGCGTAAGCGCGTGGATTGAAAC","CTCTGTTTCGCGCGCCAGGGCAGTTAGGTGCCCTAAAAGAGCGAAGTGGCCGAAAGGAAAGGCTAACGCTTCTCTAACGCTACGGCGACCTTGGCGAAATGCCATCAATACCACGC","CTCTGTTTCGCGCGCCAGGGCAGTTAGGTGCCCTAAAAGAGCGAAGTGGCCGAAAGGAAAGGCTAACGCTTCTCTAACGCTACGGCGACCTTGGCGAAATGCCATCAATACCACGC GAAAAACGCGTGGATTGAAAC","MGESVKAIKLKILDMFLDPECTKQDDNWRKDLSTMSRFCAEAGNMCLRDLYNYFSMPKEDRISSKDLYNAMYHKTKLLHPELPGKVANQIVNHAKDVWKRNAKLIYRNQISMPTYKITTAPIRLQNNIYKLIKNKNKYIIDVQLYSKEYSKDSGKGTHRYFLVAVRDSSTRMIFDRIMSKDHIDSSKSYTQGQLQIKKDHQGKWYCIIPYTFPTHETVLDPDKVMGVDLGVAKAVYWAFNSSYKRGCIDGGEIEHFRKMIRARRVSIQNQIKHSGDARKGHGRKRALKPIETLSEKEKNFRDTINHRYANRIVEAAIKQGCGTIQIENLEGIADTTGSKFLKNWPYYDLQTKIVNKAKEHGITVVAINPQYTSQRCSMCGYIEKTNRSSQAVFECKQCGYGSRTICINCRHVQVSGDVCEECGGIVKKENVNADYNAAKNISTPYIDQIIMEKCLELGIPYRSITCKECGHIQASGNTCEVCGSTNILKPKKIRKAK"]
[null,"AsCas12f1","Acidibacillus sulfuroxidans","5'-TTTA","TTTGCGAGCTAGCTTGTGGAGTGTGAAC","TCTATTCGTCGGTTCAGCGACGATAAGCCGAGAAGTGCCAATAAAACTGTTAAGTGGTTTGGTAACGCTCGGTAAGGTAGCCAAAAGGCTGAAACTCCGTGCACAAAGACCGCACGGACGCTTCACATATAGCTCATAAAC","TCTATTCGTCGGTTCAGCGACGATAAGCCGAGAAGTGCCAATAAAACTGTTAAGTGGTTTGGTAACGCTCGGTAAGGTAGCCAAAAGGCTGAAACTCCGTGCACAAAGACCGCACGGACGCTTCACATATAGCTCATAAACAAAGTTTGCGAGCTAGCTTGTGGAGTGTGAAC","MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNKWYLNISYDFEPQTRVLDLNKIMGIDLGVAVAVYMAFQHTPARYKLEGGEIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVDMAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIPNIDKIIAESIK"]
[{"$nulls":3},"* PAMs in red were predicted by CRISPRTarget"]
[{"$nulls":3},"* PAMs in green were previously validated by other groups"]