LOCUS Exported 2883 bp DNA linear UNA 23-FEB-2026 DEFINITION natural linear DNA. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 2883) AUTHORS Macapp TITLE Direct Submission JOURNAL Exported Feb 23, 2026 from SnapGene 6.0.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..2883 /mol_type="genomic DNA" /organism="unspecified" primer_bind 11..34 /label=stag1-C-geno-F1 misc_feature 127..888 /label=LHR gap 159..322 /estimated_length=164 gap 340..491 /estimated_length=152 primer_bind 683..706 /label=stag1-C-geno-F2 CDS 904..927 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 931..954 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 958..981 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 985..1008 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 1012..1035 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 1051..1107 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 1108..1992 /codon_start=1 /product="modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives" /label=HaloTag(R) /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG SEIARWLSTLEISG" misc_feature 1993..1995 /label=rhr misc_feature 1996..2735 /label=RHR primer_bind complement(2243..2267) /label=stag1-C-geno-R1 gap 2393..2453 /estimated_length=61 ORIGIN 1 aagcatttca tctacctact tcagagattc actgatattc aacaggtaat tcatcatttt 61 tgtagtaact tgataccttc caagggaagg tattttctat attgtaagtc caaattacat 121 gttttacata cagtttattc actgaaattt tgagtcttnn nnnnnnnnnn nnnnnnnnnn 181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 301 nnnnnnnnnn nnnnnnnnnn nnccaatatt tcaaaagtan nnnnnnnnnn nnnnnnnnnn 361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 481 nnnnnnnnnn ncaaaaaaag catagtaatt gaaactcttt ttgtcttttg ataattcaaa 541 tactgctgca tttttaaaat ctatacttta tatacaagcc agattctcgt tctcctttta 601 aatttaacta aatgtttcta catgtttatg tactgtcaaa aatgctagct attagtacag 661 tttttctcac aggaacaata attaaatgat tctcttttca gcccccatca agaaatcgcc 721 gagagagagc tgagctaagg ccagacttct ttgactctgc agctatcata gaagatgatt 781 cagtaaatat caaatcatta ctgcttttta tttatagttt cttggccctg gaatatatac 841 aataattgta cttctcgttt tttctaggga tttggaatgc ctatgttcga cggcattggt 901 tctgattaca aggatgacga cgataagggc gattacaagg atgacgacga taagggagat 961 tacaaggatg acgacgataa gggcgattac aaggatgacg acgataaggg agattacaag 1021 gatgacgacg ataaggtttc aggaagcgga gcaaccaact tcagcctgct gaagcaggcc 1081 ggcgacgtgg aggagaaccc cggccccgaa atcggtactg gctttccatt cgacccccat 1141 tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc 1201 cctgtgctgt tcctgcacgg taacccgacc tcctcctacg tgtggcgcaa catcatcccg 1261 catgttgcac cgacccatcg ctgcattgct ccagacctga tcggtatggg caaatccgac 1321 aaaccagacc tgggttattt cttcgacgac cacgtccgct tcatggatgc cttcatcgaa 1381 gccctgggtc tggaagaggt cgtcctggtc attcacgact ggggctccgc tctgggtttc 1441 cactgggcca agcgcaatcc agagcgcgtc aaaggtattg catttatgga gttcatccgc 1501 cctatcccga cctgggacga atggccagaa tttgcccgcg agaccttcca ggccttccgc 1561 accaccgacg tcggccgcaa gctgatcatc gatcagaacg tttttatcga gggtacgctg 1621 ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg accattaccg cgagccgttc 1681 ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa acgagctgcc aatcgccggt 1741 gagccagcga acatcgtcgc gctggtcgaa gaatacatgg actggctgca ccagtcccct 1801 gtcccgaagc tgctgttctg gggcacccca ggcgttctga tcccaccggc cgaagccgct 1861 cgcctggcca aaagcctgcc taactgcaag gctgtggaca tcggcccggg tctgaatctg 1921 ctgcaagaag acaacccgga cctgatcggc agcgagatcg cgcgctggct gtctactctg 1981 gagatttccg gttgaagtct gaagaaaatt tacaaatctg gaactctatt atttagagct 2041 agaggcctat atactgtgat agcttgtatg ggggaagaaa cgcttttgat gtgatcgggt 2101 ttgttttgta atcaaatgat taaaggttgg tcccttttgc agtgacagag cagcatgtca 2161 gtcacccatg gaaatgttgg tgaatgtcac ctcggaaaga ctgaccaaaa aaatcatttg 2221 tactctaatg ttggacttat ctcagtacag atgtgtgtgt gtttttctga aaggaggaag 2281 atattttaaa tttttaaaac agctgtcaaa ataaacactg ttatacacct gttttatgaa 2341 aatcagcatt gagtnnnnnn nntattttta actttatttt cctgttgtac aannnnnnnn 2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctatgag 2461 taagcagctc gttctaatcg ccgaagagtg tttttggagc tcgctgggtt ttgttgacct 2521 tgtaaagcag acattgaagg aacagagcat ggacaagcta aaatagagtt ctaacatcgt 2581 gcatctctgc caaaaaccac acgccctctg tggatggatg ggaatcccca gattttatat 2641 actcttgaat aaaaaggttt atttttattt ataagtgggc ataagataag tgcccatgca 2701 gccatttttc caacagatgc tgtacaccgt tcattttcta tagaatagtg agattcaaat 2761 acagtgcatt ttctattggt ctttgttctg tgcattttta gcaacttcta ccagcaaata 2821 aactattctc agtaaaacaa aaatggttct caagttatca gtctcactgt tgttaacact 2881 tgt //