{"text_format":"3","workbook":"xls","n_sheets":6,"sheets":["Supplementary Table 1","Supplementary Table 2","Supplementary Table 3","Supplementary Table 4","Supplementary Table 5","Supplementary Table 6"],"not_rendered":{"external_links":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Supplementary Table 1","index":0,"n_rows":9,"n_cols":5,"rows_kept":9,"merged":[[1,0,2,0],[1,1,2,1],[1,2,1,4],[3,0,5,0],[6,0,8,0]],"flags":["formulas_cached_only"]}
["Supplementary Table 1. RT-qPCR data for act5C and Su(Ste) transcripts in zucEY11457/+ and zucEY11457/− mutant testis enriched for SGs by over-expressing dpp"]
["Genotype","Technical trial","Ct values"]
[null,null,"Gapdh","act5C","Su(Ste) precursor transcript"]
["zucEY11457/+",1,13.27526,12.93824,14.44046]
[null,2,13.20925,12.02634,14.47205]
[null,3,13.17135,12.01301,14.72502]
["zucEY11457/−",1,12.87649,12.48631,13.00421]
[null,2,12.87127,11.56768,13.01782]
[null,3,12.76982,11.50936,13.74567]

{"sheet":"Supplementary Table 2","index":1,"n_rows":10,"n_cols":5,"rows_kept":10,"merged":[[1,0,3,0],[1,1,3,1],[1,2,1,4],[2,2,3,2],[2,4,3,4],[4,0,6,0],[7,0,9,0]],"flags":["formulas_cached_only"]}
["Supplementary Table 2. TaqMan small RNA custom assay data for piR-dme-1643 (roo piRNA) and Su(Ste)-4 piRNA in XX and XXY ovaries"]
["Genotype","Technical trial","Ct values"]
[null,null,"2S rRNA","piR-dme-1643","Su(Ste)-4 piRNA"]
[{"$nulls":3},"roo piRNA"]
["XX",1,11.28565,29.84822,">40 (undetectable)"]
[null,2,11.54246,29.46159,">40 (undetectable)"]
[null,3,11.51959,29.57925,">40 (undetectable)"]
["XXY",1,11.22979,28.59569,27.46337]
[null,2,11.15117,28.50081,27.82822]
[null,3,11.33956,28.50817,27.65261]

{"sheet":"Supplementary Table 3","index":2,"n_rows":26,"n_cols":4,"rows_kept":26,"flags":["formulas_cached_only"]}
["Supplementary Table 3. dm6 coordinates of Su(Ste) and the flanking 1360/Hoppel loci used for alignment of sequencing data in this study"]
["Su(Ste)","Genomic strand","1360/Hoppel","Genomic strand"]
["chrY:939935-940435","+","chrY:940435-940551","-"]
["chrY:1005985-1007312","+","chrY:1007312-1007915","-"]
["chrY:1033830-1035493","+","chrY:1035493-1035716","-"]
["chrY:1035826-1036823","+","chrY:1036823-1037851","-"]
["chrY:2559808-2561503","+","chrY:2561503-2562677","-"]
["chrY:2578088-2578557","+","chrY:2578557-2579710","-"]
["chrY:2590754-2592449","+","chrY:2592449-2593623","-"]
["chrY:2565516-2567202","+","chrY:2567202-2568355","-"]
["chrY:2568355-2570041","+","chrY:2570041-2571194","-"]
["chrY:2593623-2595309","+","chrY:2595309-2596462","-"]
["chrY:2562677-2564363","+","chrY:2564363-2565516","-"]
["chrY:896182-897725","-","chrY:895866-896182","+"]
["chrY:903078-904499","-","chrY:902114-903078","+"]
["chrY:906804-907496","-","chrY:905678-906804","+"]
["chrY:934057-935745","-","chrY:933409-934057","+"]
["chrY:1032219-1032946","-","chrY:1031620-1032219","+"]
["chrY_DS483875v1_random:2047-3374","-","chrY_DS483875v1_random:1444-2047","+"]
["chrY_DS483742v1_random:400-2013","+","chrY_DS483742v1_random:2013-2243","-"]
["chrY:949253-950723","+","chrY:950723-951071","-"]
["chrY:2514981-2516667","+","chrY:2516667-2517847","-"]
["chrY:2540804-2542491","+","chrY:2542491-2543673","-"]
["chrY:2571195-2542491","+","chrY: 2542491-2574039","-"]
["chrY:904757-905678","-","chrY:904537-904757","+"]
["chrY:978543-980284","-","chrY:977621-978543","+"]

{"sheet":"Supplementary Table 4","index":3,"n_rows":3,"n_cols":3,"rows_kept":3,"flags":["formulas_cached_only"]}
["Supplementary Table 4. cDNA sequence used for UAS-GFP-Ste construct"]
["Gene name","Gene ID","cDNA sequence"]
["SteXh:CG42398",7354447,"atgtcgagctcccagaacaacaacagcagctggatcgattggttcctcgggatcaagggcaacgagttcctctgccgcgtgcccaccgactacgtgcaggatacgttcaaccagatgggcttggagtatttcagcgagatactggacgtgatcctgaagccggtgatcgacagttcctctggcttgttgtacggcgatgaaaaaaagtggtacggcatgattcacgcccgatacatcaagtcagagcgtggcgtgattgctatgcaccgaaaatatatgcgaggagattttgaatcgtgtcccaatatctcctgtgataggcagaacaccctgccagtgggcctcagcgatgtatggggcaagtcaaccgtcaagatttactgcccacggtgtaaaaagaactttcatccgaagtctgatacacagctggacggagcgatgttcgggcccagcttcccggacatcttcttctcgctgctgccgaacttgagatcgcccctggacgacccacgtacctag"]

{"sheet":"Supplementary Table 5","index":4,"n_rows":27,"n_cols":4,"rows_kept":27,"merged":[[2,0,24,0]],"flags":["formulas_cached_only"]}
["Supplementary Table 5. smRNA-FISH probes"]
[null,"DNA oligo sequence","5’ modification","3’ modification"]
["ste probe mix","ccagttcacttgttcacag","-","Quasar 570"]
[null,"tctgggagctcgacatgtt","-","Quasar 570"]
[null,"ttgatcccgaggaaccaat","-","Quasar 570"]
[null,"cggcagaggaactcgttgc","-","Quasar 570"]
[null,"tatcctgcacgtagtcggt","-","Quasar 570"]
[null,"caagcccatctggttgaac","-","Quasar 570"]
[null,"tccagtatctcgctgaaat","-","Quasar 570"]
[null,"tcaccggcttcaggatcac","-","Quasar 570"]
[null,"caagccagaggaactgtcg","-","Quasar 570"]
[null,"tttttttcatcgccgtaca","-","Quasar 570"]
[null,"cgtgaatcatgccgtacca","-","Quasar 570"]
[null,"ctctgacttgatgtatcgg","-","Quasar 570"]
[null,"tgcatagcaatcacgccac","-","Quasar 570"]
[null,"ctccttgcatatattttcg","-","Quasar 570"]
[null,"ggagatattgggacacgat","-","Quasar 570"]
[null,"agggtgttctgcctatcac","-","Quasar 570"]
[null,"atcttgacggttgacttgc","-","Quasar 570"]
[null,"gctgtgtatcagacttcgg","-","Quasar 570"]
[null,"gaagctgggcccgaacatc","-","Quasar 570"]
[null,"agcgagaagaagatgtccg","-","Quasar 570"]
[null,"caggggcgatctcaagttc","-","Quasar 570"]
[null,"cgaaagcctaggtaccgtg","-","Quasar 570"]
[null,"tttgggcgatttgagttgc","-","Quasar 570"]
["Su(Ste) antisense probe ","attccgaagtcaagcgcttcaat","Cy5 or Cy3","-"]
["Su(Ste) sense probe","acgtgtcctgaaagtagtt","Cy3","-"]

{"sheet":"Supplementary Table 6","index":5,"n_rows":24,"n_cols":6,"rows_kept":24,"merged":[[2,0,9,0],[2,1,4,1],[5,1,7,1],[8,1,9,1],[10,0,12,0],[10,1,12,1],[13,0,23,0],[13,1,14,1],[15,1,17,1],[18,1,20,1],[21,1,23,1]],"flags":["formulas_cached_only"]}
["Supplementary Table 6. Summary of sequencing data"]
["Library type","Sample","Sample","Total RNA, µg","Amount of spike-in, attomol","Raw sequencing reads"]
["Small RNA-seq","XX ovaries (0-5 days old y1w1118 virgin females)",1,4.5,2000,{"v":29980053,"d":"29,980,053"}]
[null,null,2,4.2,2000,{"v":43147777,"d":"43,147,777"}]
[null,null,3,3.85,2000,{"v":32877306,"d":"32,877,306"}]
[null,"XXY ovaries (0-5 days old y1w1118/C(X:Y)y1f1w1; nos-gal4:VP16 /+ virgin females)",1,3.65,2000,{"v":20386472,"d":"20,386,472"}]
[null,null,2,3.4,2000,{"v":31245993,"d":"31,245,993"}]
[null,null,3,2.8,2000,{"v":56513524,"d":"56,513,524"}]
[null,"XY testis (0-5 days old y1w1118/Y; nos-gal4:VP16/TM2 males)",1,6,2000,{"v":14863778,"d":"14,863,778"}]
[null,null,2,4.9,2000,{"v":13683972,"d":"13,683,972"}]
["Stranded RNA-seq","zucEY11457/− mutant XY testis enriched for SGs by overexpressing dpp (0-5 days old y1w1118/Y; zucEY11457/Df(2L)BSC323; nos-gal4:VP16/UAS-Dpp males)",1,1.5,"-",{"v":25037519,"d":"25,037,519"}]
[null,null,2,1.5,"-",{"v":20622202,"d":"20,622,202"}]
[null,null,3,1.5,"-",{"v":24682579,"d":"24,682,579"}]
["5′-monophosphate RNA-seq","armi control XY testis (0-5 days old y1w1118/Y; nos-gal4:VP16/TM2 males)",1,2,"-",{"v":19028472,"d":"19,028,472"}]
[null,null,2,2,"-",{"v":20298882,"d":"20,298,882"}]
[null,"armiRNAi XY testis (0-5 days old y1w1118/Y; nos-gal4:VP16/armiTRIP.GL00254 males)",1,2,"-",{"v":17546956,"d":"17,546,956"}]
[null,null,2,2,"-",{"v":20234916,"d":"20,234,916"}]
[null,null,3,2,"-",{"v":21488474,"d":"21,488,474"}]
[null,"control XY testis enriched for SGs by overexpressing dpp (0-5 days old y1w1118/Y; zucEY11457/CyO; nos-gal4:VP16/UAS-Dpp males)",1,2,"-",{"v":5846395,"d":"5,846,395"}]
[null,null,2,2,"-",{"v":12974619,"d":"12,974,619"}]
[null,null,3,2,"-",{"v":18788561,"d":"18,788,561"}]
[null,"zucEY11457/− mutant XY testis enriched for SGs by overexpressing dpp (0-5 days old y1w1118/Y; zucEY11457/Df(2L)BSC323; nos-gal4:VP16/UAS-Dpp males)",1,2,"-",{"v":22210754,"d":"22,210,754"}]
[null,null,2,2,"-",{"v":18020998,"d":"18,020,998"}]
[null,null,3,2,"-",{"v":20136122,"d":"20,136,122"}]