{"text_format":"3","workbook":"xls","n_sheets":4,"sheets":["ABs_table","Image cycling strategy","Sample Info 1","Sample Info 2"],"not_rendered":{"external_links":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"ABs_table","index":0,"n_rows":64,"n_cols":12,"rows_kept":64,"flags":["formulas_cached_only"]}
["AB applied in cycle","AB applied in cycle in permuted condition","gene ID","Stain was excluded from analysis","Species","color channel","EnsemblID","clonality","company","AB ID","Dilution [1:x ul]","Collagen masked"]
[1,7,"DNM1",null,"Ms","red","ENSG00000087470","monoclonal","Thermo","MA5-15285",200]
[2,5,"SV2A",null,"Ms","red","ENSG00000159164","monoclonal","Developmental Studies Hybridoma Bank","NA",400]
[3,3,"NPC",null,"Ms","red","group of genes","monoclonal","Abcam","ab24609",400,"X"]
[4,6,"YAP1",null,"Ms","red","ENSG00000137693","monoclonal","Abcam","ab56701",400,"X"]
[5,2,"TUBG1",null,"Ms","red","ENSG00000131462","Monoclonal","Sigma","T5326",400]
[6,1,"HSPD1",null,"Ms","red","ENSG00000144381","monoclonal","Abcam","ab13532",1000]
[7,4,"COL2A1",null,"Ms","red","ENSG00000139219","monoclonal","origene","BM332",200]
[8,14,"CCNE1",null,"Ms","red","ENSG00000105173","monoclonal","Abcam","ab3927",150]
[9,10,"ACTB",null,"Ms","red","ENSG00000075624","monoclonal","Abcam","ab6276",1000]
[10,11,"RB1","excluded","Ms","red","ENSG00000139687","monoclonal","BD Biosciences",554136,400]
[11,13,"BSN",null,"Ms","red","ENSG00000164061","monoclonal","Enzo","SAP7F407",800,"X"]
[12,12,"CTNNB1",null,"Ms","red","ENSG00000168036","monoclonal","BD Transduction Laboratories",610154,300]
[13,9,"MAP2",null,"Ms","red","ENSG00000078018","polyclonal","Millipore","AB5622",1000,"X"]
[14,8,"p-EGFR","excluded","Ms","red","ENSG00000146648","polyclonal","ThermoFischer Scientific","36-9700",200]
[15,16,"PAX6",null,"Ms","red","ENSG00000007372","monoclonal","ATLAS","AMAb91372",300,"X"]
[16,20,"TUBB3",null,"Ms","red","ENSG00000258947","monoclonal","millipore","MAB1637",100]
[17,18,"GRIA2",null,"Ms","red","ENSG00000120251","monoclonal","Zymed","32-0300",100,"X"]
[18,15,"NEUROD1",null,"Ms","red","ENSG00000162992","monoclonal","Abcam","ab60704",300,"X"]
[19,19,"TUBA4A",null,"Ms","red","ENSG00000127824","monoclonal","Sigma","T5168",400]
[20,17,"RHO",null,"Ms","red","ENSG00000163914","monoclonal","sigma","R5403",1000]
[21,21,"RLBP1",null,"Ms","red","ENSG00000140522","monoclonal","Santz Cruz","sc-59487",50]
[1,7,"CDH2",null,"Sp","far_red","ENSG00000170558","polyclonal","R&D Systems","AF6426",400]
[2,5,"EOMES","excluded","Ck","far_red","ENSG00000163508","polyclonal","Millipore","AB15894",1000]
[3,3,"GLRA2",null,"Gt","far_red","ENSG00000101958","polyclonal","Santa Cruz Biotechnology","sc-17279",50,"X"]
[4,6,"SLC32A1",null,"Gp","far_red","ENSG00000101438","polyclonal","Synaptic Systems",131004,400]
[5,2,"NRL",null,"Gt","far_red","ENSG00000129535","polyclonal","R&D systems","AF2945-SP",50]
[6,1,"SLC17A7",null,"Gp","far_red","ENSG00000104888","polyclonal","Chemicon","AB5905",1000]
[7,4,"Serotonin",null,"Gt","far_red","not a gene product","polyclonal","Abcam","ab66047",400]
[8,14,"RBFOX3","excluded","Gp","far_red","ENSG00000167281","monoclonal","Synaptic Systems","266 011",1000]
[9,10,"EGFR","excluded","Gt","far_red","ENSG00000146648","polyclonal","R&D Systems","AF231",200]
[10,11,"EPHB2",null,"Gt","far_red","ENSG00000133216","polyclonal","R&D Systems","AF467",1000]
[11,13,"CALB1",null,"Ck","far_red","ENSG00000104327","polyclonal","Novus Biologicals","NBP2-50028SS",1000]
[12,12,"CHRNA7","excluded","Rt","far_red","ENSG00000175344","polyclonal","Santa Cruz Biotechnology"," sc-58607",50]
[13,9,"MAPT",null,"Gp","far_red","ENSG00000186868","polyclonal","Synaptic Systems","314 004",500]
[14,8,"VSX2",null,"Sp","far_red","ENSG00000119614","polyclonal","exalpha","X1180P",400]
[15,16,"excluded",null,"Gt","far_red","ENSG00000164458","polyclonal","R&D systems","AF2085",50]
[16,20,"Nucleoli Marker Antibody",null,"Gt","far_red","NA","NA","NA","NA",400,"X"]
[17,18,"SLC18A3","excluded","Gt","far_red","ENSG00000187714","polyclonal","Abcam","ab27941",200]
[18,15,"mAChR M5 (N-19)","excluded","Gt","far_red","ENSG00000184984","polyclonal","Santa Cruz Biotechnology","sc-7479",50]
[19,19,"POU4F2",null,"Gt","far_red","ENSG00000152192","polyclonal","Santz Cruz","sc-6026",100]
[20,17,"ONECUT2",null,"Sp","far_red","ENSG00000119547","polyclonal","R&D systems","AF6294",200]
[21,21,"excluded GABA","excluded","Gp","far_red","not a gene product","polyclonal","merck millipore","AB175",200]
[1,7,"MAPK1_MAPK3",null,"Rb","green","ENSG00000100030","polyclonal","Cell Signaling Technology",9101,300]
[2,5,"HES1",null,"Rb","green","ENSG00000114315","monoclonal","Cell signaling",11988,300]
[3,3,"PCNA",null,"Rb","green","ENSG00000132646","monoclonal","Cell Signaling Technology",13110,300]
[4,6,"SOX9",null,"Rb","green","ENSG00000125398","monoclonal","Abcam","ab185966",1000]
[5,2,"GFAP",null,"Rb","green","ENSG00000131095","polyclonal","abcam","ab7260",400]
[6,1,"DDX6",null,"Rb","green","ENSG00000110367","polyclonal","Bethyl Laboratories","A300-461A",5000]
[7,4,"KCNJ10",null,"Rb","green","ENSG00000177807","polyclonal","Proteintech","12503-1-AP",400]
[8,14,"CLUAP1",null,"Rb","green","ENSG00000103351","polyclonal","Proteintech","17470-1-AP",200]
[9,10,"GPHN",null,"Rb","green","ENSG00000171723","polyclonal","Chemicon (Millipore)","AB5725",200]
[10,11,"GRIA4",null,"Rb","green","ENSG00000152578","polyclonal","Millipore","AB1508",200]
[11,13,"OPN1SW",null,"Rb","green","ENSG00000128617","polyclonal","merck millipore","AB5407",300]
[12,12,"OPN1LW",null,"Rb","green","ENSG00000102076","polyclonal","merck millipore","AB5405",400]
[13,9,"CALR",null,"Rb","green","ENSG00000179218","polyclonal","Abcam",2907,300]
[14,8,"AIPL1",null,"Rb","green","ENSG00000129221","polyclonal","ProteinTech","15108-1-AP",200]
[15,16,"ATP1A1",null,"Rb","green","ENSG00000163399","monoclonal","abcam","ab76020",100]
[16,20,"CTBP2",null,"Rb","green","ENSG00000175029","polyclonal","Synaptic Systems",192103,800]
[17,18,"MKI67",null,"Rb","green","ENSG00000148773","monoclonal","abcam","ab16667",200]
[18,15,"PRKCA",null,"Rb","green","ENSG00000154229","monoclonal","abcam","ab32376",100]
[19,19,"IMPDH1",null,"Rb","green","ENSG00000106348","polyclonal","Proteintech","22092-1-AP",200]
[20,17,"NES",null,"Rb","green","ENSG00000132688","polyclonal","Sigma","N5413",200]
[21,21,"RCVRN",null,"Rb","green","ENSG00000109047","polyclonal","merck millipore","AB5585",1000]

{"sheet":"Image cycling strategy","index":1,"n_rows":29,"n_cols":4,"rows_kept":29,"flags":["formulas_cached_only"]}
["true cycle","imaging cycle (naming convention)"]
[1,0,"imaging of unstained tissues"]
[2,1,"elution + staining according to 4i protocol; imaging"]
[3,2,"elution + staining according to 4i protocol; imaging"]
[4,3,"elution + staining according to 4i protocol; imaging"]
[5,4,"elution + staining according to 4i protocol; imaging"]
[6,5,"elution + staining according to 4i protocol; imaging"]
[7,"5_0","elution and mock staining without antibodies according to 4i protocol; imaging"]
[8,6,"elution + staining according to 4i protocol; imaging"]
[9,7,"elution + staining according to 4i protocol; imaging"]
[10,8,"elution + staining according to 4i protocol; imaging"]
[11,9,"elution + staining according to 4i protocol; imaging"]
[12,10,"elution + staining according to 4i protocol; imaging"]
[13,"10_0","elution and mock staining without antibodies according to 4i protocol; imaging"]
[14,11,"elution + staining according to 4i protocol; imaging"]
[15,12,"elution + staining according to 4i protocol; imaging"]
[16,13,"elution + staining according to 4i protocol; imaging"]
[17,14,"elution + staining according to 4i protocol; imaging"]
[18,15,"elution + staining according to 4i protocol; imaging"]
[19,"15_0","elution and mock staining without antibodies according to 4i protocol; imaging"]
[20,16,"elution + staining according to 4i protocol; imaging"]
[21,17,"elution + staining according to 4i protocol; imaging"]
[22,18,"elution + staining according to 4i protocol; imaging"]
[23,19,"elution + staining according to 4i protocol; imaging"]
[24,20,"elution + staining according to 4i protocol; imaging"]
[25,"20_0","elution and mock staining without antibodies according to 4i protocol; imaging"]
[26,21,"elution + staining according to 4i protocol; imaging"]
[27,"1_2","elution + staining according to 4i protocol; imaging","replicate staining of imaging cycle 1"]
[28,"1_3","elution + staining according to 4i protocol; imaging","replicate staining of imaging cycle 1"]

{"sheet":"Sample Info 1","index":2,"n_rows":48,"n_cols":6,"rows_kept":48,"flags":["formulas_cached_only"]}
["section ID (consistent with eyesee4is.ethz.ch)","Kind","Age [weeks]","Antibody order","sample ID","Cell line/ sample"]
[2,"sample",6,1,2,"01F49i-N-B7"]
[4,"sample",6,1,4,"01F49i-N-B7"]
[5,"sample",12,1,5,"01F49i-N-B7"]
[6,"sample",12,1,6,"01F49i-N-B7"]
[7,"sample",12,1,7,"01F49i-N-B7"]
[8,"sample",12,1,8,"01F49i-N-B7"]
[10,"control odd","adult primary",1,"-","​​R-00646_06"]
[11,"sample",6,2,2,"01F49i-N-B7"]
[12,"sample",6,2,4,"01F49i-N-B7"]
[14,"sample",6,2,3,"01F49i-N-B7"]
[15,"sample",6,2,4,"01F49i-N-B7"]
[16,"sample",12,2,6,"01F49i-N-B7"]
[17,"sample",12,2,5,"01F49i-N-B7"]
[18,"sample",12,2,7,"01F49i-N-B7"]
[19,"sample",12,2,8,"01F49i-N-B7"]
[20,"sample",24,2,9,"01F49i-N-B7"]
[21,"sample",24,2,10,"01F49i-N-B7"]
[22,"sample",24,2,11,"01F49i-N-B7"]
[23,"sample",24,2,9,"01F49i-N-B7"]
[24,"sample",24,2,10,"01F49i-N-B7"]
[25,"sample",24,2,11,"01F49i-N-B7"]
[26,"sample",18,2,12,"01F49i-N-B7"]
[27,"sample",18,2,13,"01F49i-N-B7"]
[28,"sample",18,2,12,"01F49i-N-B7"]
[29,"sample",18,2,13,"01F49i-N-B7"]
[30,"control",39,1,"-","01F49i-N-B7"]
[31,"control",39,1,"-","01F49i-N-B7"]
[32,"sample",24,1,9,"01F49i-N-B7"]
[34,"sample",24,1,11,"01F49i-N-B7"]
[35,"sample",24,1,9,"01F49i-N-B7"]
[39,"sample",18,1,12,"01F49i-N-B7"]
[41,"sample",18,1,12,"01F49i-N-B7"]
[43,"sample",39,1,14,"01F49i-N-B7"]
[44,"sample",39,1,15,"01F49i-N-B7"]
[45,"sample",39,1,15,"01F49i-N-B7"]
[46,"control odd","adult primary",2,"-","​​R-00646_06"]
[48,"sample",39,2,14,"01F49i-N-B7"]
[49,"sample",39,2,14,"01F49i-N-B7"]
[50,"sample",39,2,15,"01F49i-N-B7"]
[51,"sample",39,2,15,"01F49i-N-B7"]
[54,"sample",39,2,16,"01F49i-N-B7"]
[55,"sample",39,2,16,"01F49i-N-B7"]
[56,"control even",39,2,"-","01F49i-N-B7"]
[57,"control even",39,2,"-","01F49i-N-B7"]
[60,"sample",39,1,17,"01F49i-N-B7"]
[61,"sample",39,1,17,"01F49i-N-B7"]
[72,"sample","adult primary",1,18,"​​R-00646_06"]

{"sheet":"Sample Info 2","index":3,"n_rows":18,"n_cols":6,"rows_kept":18,"flags":["formulas_cached_only"]}
["tissue ID ","tissue kind","age","# sections","# AB order 1","# AB order 2"]
[2,"organoid",6,2,1,1]
[3,"organoid",6,1,0,1]
[4,"organoid",6,3,1,2]
[5,"organoid",12,2,1,1]
[6,"organoid",12,2,1,1]
[7,"organoid",12,2,1,1]
[8,"organoid",12,2,1,1]
[9,"organoid",24,4,2,2]
[10,"organoid",24,2,2,0]
[11,"organoid",24,3,1,2]
[12,"organoid",18,4,2,2]
[13,"organoid",18,2,0,2]
[14,"organoid",39,3,1,2]
[15,"organoid",39,4,2,2]
[16,"organoid",39,2,0,2]
[17,"organoid",39,2,2,0]
[18,"primary retina",">60 years",1,1,0]