LOCUS CRISPR-StAR3_(Re 8187 bp DNA circular SYN 23-MAY-2024 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8187) AUTHORS esther.uijttewaal TITLE Direct Submission JOURNAL Exported May 23, 2024 from SnapGene 7.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8187 /mol_type="other DNA" /organism="recombinant plasmid" LTR 1..684 /label=5'LTR misc_feature 555 /label=T /label=nonstandard type: Editing History Replacement misc_feature 690..1568 /label=Psi+ misc_feature 1666..1906 /label=U6 promoter misc_feature 1939..1955 /label=Tracr misc_recomb 1956..1989 /label=LoxP misc_feature 1990..2000 /label=stop misc_feature 2117..2157 /label=lox5171+PacI site misc_feature 2117..2149 /label=Lox5171 misc_recomb 2174..2207 /label=LoxP misc_feature 2230..2729 /label=mouse pgk CDS 2743..3141 /codon_start=1 /label=blast /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_recomb 3150..3183 /label=LoxP /label=LoxP(1) misc_feature 3184..3259 /label=Tracr + stop misc_feature 3263..3301 /label=lox5171+XbaI site misc_feature 3263..3295 /label=Lox5171 misc_feature 3406..3905 /label=mouse pgk CDS 3919..4722 /label=Neo LTR 4840..5460 /label=LTR-SIN CDS complement(6965..7825) /label=AMP misc_feature complement(7867..7895) /label=AMP promoter ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc 241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact 541 cggcgcgcca gtctcccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct 601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga 661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg 721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt 781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact 841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc 901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt 961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac 1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc 1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt 1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag 1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct 1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc 1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag 1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca 1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc 1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc 1561 taggcgccgg aattgaaggt cgtagatctc cgcggttaat taaaatgata cggcgaccac 1621 cgagatctgt cttgatctag gagctactca tcaacggtca catgtgaggg cctatttccc 1681 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat 1741 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt 1801 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact 1861 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct 1921 tcgctcgaga gaagacctgt ttaagagcta tgctgataac ttcgtatagc atacattata 1981 cgaagttatt tttttttttt gcggccgcca agagagcaga aatagcaagt aactcattag 2041 gtaaataagg tagtccgttt agtgccgtag ccaattctca gacatttaat ggctctaaac 2101 gtaacattta caaattataa cttcgtataa tgtgtactat acgaagttat taattaattc 2161 tggttcgaac ctgataactt cgtatagcat acattatacg aagttatcat tcgaatgtag 2221 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag 2281 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca 2341 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc 2401 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg 2461 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg 2521 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc 2581 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc 2641 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt 2701 tctcctcttc ctcatctccg ggcctttcga cctgcagcca atatggccaa gcctttgtct 2761 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct 2821 gaggattaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc 2881 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct 2941 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc 3001 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc 3061 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct 3121 ggttatgtgt gggagggctg acctgcagga taacttcgta tagcatacat tatacgaagt 3181 tatcagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga 3241 gtcggtgctt ttttgtttta gcataacttc gtataatgtg tactatacga agttatctag 3301 aggtacccgt taagatcgga agagcacacg tctgaactcc agtcacaaaa ttatctcgta 3361 tgccgtctta tgcttgttaa ttaacaattg acgcgtaatt ctaccgggta ggggaggcgc 3421 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca 3481 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt 3541 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg 3601 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg 3661 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat 3721 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg 3781 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat 3841 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc 3901 tttcgacctg cagccaatat gggatcggcc attgaacaag atggattgca cgcaggttct 3961 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 4021 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 4081 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc 4141 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 4201 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 4261 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 4321 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 4381 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 4441 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 4501 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 4561 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag 4621 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 4681 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatcc gtcgacctgc 4741 agccaagctt atcgataccg tcgagattta attaagatat catcagtggt ccaggctcta 4801 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa 4861 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc 4921 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag 4981 aagttcagat caaggtcagg aacagatgga acagggtcga ccctagagaa ccatcagatg 5041 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag 5101 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa 5161 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca 5221 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct 5281 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg 5341 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt 5401 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 5461 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 5521 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtagat ccggctgtgg 5581 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 5641 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 5701 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 5761 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 5821 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 5881 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac tatgcggcat 5941 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 6001 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6061 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 6661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 7201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 7261 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 7321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 7381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 7441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 7501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 7561 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag 7621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 7681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 7741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 7801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 7861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 7921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 7981 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctacaa gaattagctt 8041 ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa 8101 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt 8161 cattagttca tagcccatat atggagt //