LOCUS CRISPR-StAR4_(Re 8141 bp DNA circular SYN 23-MAY-2024 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8141) AUTHORS esther.uijttewaal TITLE Direct Submission JOURNAL Exported May 23, 2024 from SnapGene 7.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8141 /mol_type="other DNA" /organism="recombinant plasmid" LTR 1..684 /label=5'LTR misc_feature 690..1568 /label=Psi+ misc_feature 1666..1906 /label=U6 promoter misc_feature 1939..1955 /label=Tracr misc_recomb 1956..1989 /label=LoxP misc_feature 1990..2000 /label=stop misc_feature 2117..2157 /label=lox5171+PacI site misc_feature 2117..2149 /label=Lox5171 misc_feature 2184..2683 /label=mouse pgk CDS 2697..3095 /codon_start=1 /label=blast /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_recomb 3104..3137 /label=LoxP /label=LoxP(1) misc_feature 3138..3213 /label=Tracr + stop misc_feature 3217..3255 /label=lox5171+XbaI site misc_feature 3217..3249 /label=Lox5171 misc_feature 3360..3859 /label=mouse pgk CDS 3873..4676 /label=Neo LTR 4794..5414 /label=LTR-SIN CDS complement(6919..7779) /label=AMP misc_feature complement(7821..7849) /label=AMP promoter ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc 241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact 541 cggcgcgcca gtctcccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct 601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga 661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg 721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt 781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact 841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc 901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt 961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac 1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc 1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt 1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag 1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct 1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc 1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag 1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca 1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc 1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc 1561 taggcgccgg aattgaaggt cgtagatctc cgcggttaat taaaatgata cggcgaccac 1621 cgagatctgt cttgatctag gagctactca tcaacggtca catgtgaggg cctatttccc 1681 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat 1741 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt 1801 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact 1861 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct 1921 tcgctcgaga gaagacctgt ttaagagcta tgctgataac ttcgtatagc atacattata 1981 cgaagttatt tttttttttt gcggccgcca agagagcaga aatagcaagt aactcattag 2041 gtaaataagg tagtccgttt agtgccgtag ccaattctca gacatttaat ggctctaaac 2101 gtaacattta caaattataa cttcgtataa tgtgtactat acgaagttat taattaattc 2161 tggttcgaat gtagaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc 2221 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac 2281 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc 2341 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag 2401 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga 2461 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt 2521 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg 2581 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac 2641 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaatatgg 2701 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca 2761 gcatccccat ctctgaggat tacagcgtcg ccagcgcagc tctctctagc gacggccgca 2821 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc 2881 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg 2941 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc 3001 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg 3061 aattgctgcc ctctggttat gtgtgggagg gctgacctgc aggataactt cgtatagcat 3121 acattatacg aagttatcag catagcaagt ttaaataagg ctagtccgtt atcaacttga 3181 aaaagtggca ccgagtcggt gcttttttgt tttagcataa cttcgtataa tgtgtactat 3241 acgaagttat ctagaggtac ccgttaagat cggaagagca cacgtctgaa ctccagtcac 3301 aaaattatct cgtatgccgt cttatgcttg ttaattaaca attgacgcgt aattctaccg 3361 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 3421 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 3481 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 3541 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 3601 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 3661 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 3721 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 3781 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 3841 ctcatctccg ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat 3901 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 3961 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 4021 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 4081 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 4141 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 4201 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 4261 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 4321 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 4381 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 4441 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 4501 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 4561 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 4621 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg 4681 atccgtcgac ctgcagccaa gcttatcgat accgtcgaga tttaattaag atatcatcag 4741 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc 4801 catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca 4861 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat 4921 aactgagaat agagaagttc agatcaaggt caggaacaga tggaacaggg tcgaccctag 4981 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 5041 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat 5101 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg 5161 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct 5221 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct 5281 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg 5341 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 5401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 5461 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 5521 agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 5581 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 5641 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 5701 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 5761 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 5821 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttactggct 5881 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 5941 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga 6001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 6061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 6121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 6181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 6301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 6361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 6421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 6481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 6541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 6601 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 6721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 6781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 6841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 6901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 6961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 7021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 7081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 7141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 7201 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc 7261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 7321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 7381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 7441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 7501 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag 7561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 7621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 7681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 7741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 7801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 7861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 7921 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 7981 acaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt acatttatat 8041 tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta 8101 atcaattacg gggtcattag ttcatagccc atatatggag t //