LOCUS       CRISPR-StAR4_(Re        8141 bp DNA     circular SYN 23-MAY-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   esther.uijttewaal
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8141
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             1..684
                     /label=5'LTR
     misc_feature    690..1568
                     /label=Psi+
     misc_feature    1666..1906
                     /label=U6 promoter
     misc_feature    1939..1955
                     /label=Tracr
     misc_recomb     1956..1989
                     /label=LoxP
     misc_feature    1990..2000
                     /label=stop
     misc_feature    2117..2157
                     /label=lox5171+PacI site
     misc_feature    2117..2149
                     /label=Lox5171
     misc_feature    2184..2683
                     /label=mouse pgk
     CDS             2697..3095
                     /codon_start=1
                     /label=blast
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_recomb     3104..3137
                     /label=LoxP
                     /label=LoxP(1)
     misc_feature    3138..3213
                     /label=Tracr + stop
     misc_feature    3217..3255
                     /label=lox5171+XbaI site
     misc_feature    3217..3249
                     /label=Lox5171
     misc_feature    3360..3859
                     /label=mouse pgk
     CDS             3873..4676
                     /label=Neo
     LTR             4794..5414
                     /label=LTR-SIN
     CDS             complement(6919..7779)
                     /label=AMP
     misc_feature    complement(7821..7849)
                     /label=AMP promoter
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
      541 cggcgcgcca gtctcccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
      601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
      721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
      781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
      841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1561 taggcgccgg aattgaaggt cgtagatctc cgcggttaat taaaatgata cggcgaccac
     1621 cgagatctgt cttgatctag gagctactca tcaacggtca catgtgaggg cctatttccc
     1681 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     1741 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     1801 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     1861 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct
     1921 tcgctcgaga gaagacctgt ttaagagcta tgctgataac ttcgtatagc atacattata
     1981 cgaagttatt tttttttttt gcggccgcca agagagcaga aatagcaagt aactcattag
     2041 gtaaataagg tagtccgttt agtgccgtag ccaattctca gacatttaat ggctctaaac
     2101 gtaacattta caaattataa cttcgtataa tgtgtactat acgaagttat taattaattc
     2161 tggttcgaat gtagaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     2221 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     2281 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     2341 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     2401 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     2461 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     2521 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     2581 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     2641 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaatatgg
     2701 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     2761 gcatccccat ctctgaggat tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     2821 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     2881 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     2941 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     3001 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     3061 aattgctgcc ctctggttat gtgtgggagg gctgacctgc aggataactt cgtatagcat
     3121 acattatacg aagttatcag catagcaagt ttaaataagg ctagtccgtt atcaacttga
     3181 aaaagtggca ccgagtcggt gcttttttgt tttagcataa cttcgtataa tgtgtactat
     3241 acgaagttat ctagaggtac ccgttaagat cggaagagca cacgtctgaa ctccagtcac
     3301 aaaattatct cgtatgccgt cttatgcttg ttaattaaca attgacgcgt aattctaccg
     3361 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     3421 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     3481 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     3541 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     3601 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     3661 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     3721 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     3781 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     3841 ctcatctccg ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat
     3901 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     3961 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4021 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     4081 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4141 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4201 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4261 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4321 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4381 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     4441 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4501 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4561 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4621 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     4681 atccgtcgac ctgcagccaa gcttatcgat accgtcgaga tttaattaag atatcatcag
     4741 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc
     4801 catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
     4861 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
     4921 aactgagaat agagaagttc agatcaaggt caggaacaga tggaacaggg tcgaccctag
     4981 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     5041 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     5101 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     5161 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     5221 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     5281 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     5341 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5461 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     5521 agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5581 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5641 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5701 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5761 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5821 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttactggct
     5881 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     5941 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     6001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6601 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7201 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     7261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7501 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     7561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     7921 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     7981 acaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt acatttatat
     8041 tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta
     8101 atcaattacg gggtcattag ttcatagccc atatatggag t
//
