LOCUS CRISPR-StAR4B_(L 9742 bp DNA circular SYN 23-MAY-2024 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9742) AUTHORS esther.uijttewaal TITLE Direct Submission JOURNAL Exported May 23, 2024 from SnapGene 7.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..9742 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 190..1050 /label=Amp misc_feature 1092..1130 /gene="Bacterial-Promoter prom" /label=Bacterial-Promoter prom misc_feature complement(1092..1120) /direction=LEFT /label=AMP promoter /label=nonstandard type: promotor misc_feature 1523..1792 /label=CAG_enhancer misc_feature 2022..2203 /gene="RU5" /label=RU5 misc_signal 2313..2357 /label=psi misc_feature 2358..2710 /label=Gag /note="Gag" misc_feature 2772..2787 /label=SV40_int misc_feature 2861..3159 /label=RRE misc_feature 3628..3751 /label=cPPT /label=cPPT; cPPT/CTS misc_feature 3771..3781 /label=Psi+ misc_feature 3872..4112 /label=U6 promoter misc_feature 4148..4163 /label=Tracr misc_recomb 4164..4197 /label=LoxP misc_feature 4198..4208 /label=stop misc_feature 4325..4365 /label=lox5171+PacI site misc_feature 4325..4357 /label=Lox5171 misc_feature 4392..4891 /label=mouse pgk CDS 4905..5303 /codon_start=1 /label=blast /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_recomb 5312..5345 /label=LoxP /label=LoxP(1) misc_feature 5346..5421 /label=Tracr + stop misc_feature 5425..5463 /label=lox5171+XbaI site misc_feature 5425..5457 /label=Lox5171 misc_binding 5617..6205 /label=WPRE misc_feature 6393..6408 /label=cPPT misc_feature 6409..6462 /label=delta U3 LTR 6676..6911 /label=3' LTR SIN CDS 6918..7149 /label=bGH polyA misc_feature 7229..7535 /label=f1 Origin promoter 7667..7935 /label=SV40_promoter gene 8097..8468 /gene="bleo" /label=bleo terminator 8604..8735 /label=SV40_PA_terminator rep_origin complement(9120..9340) /direction=LEFT /label=PMB1 ORIGIN 1 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 61 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 1021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 1081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 1141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga 1201 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 1261 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa 1321 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt 1381 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgcg cgttgacatt gattattgac 1441 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 1501 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 1561 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 1621 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 1681 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 1741 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 1801 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 1861 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 1921 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 1981 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc 2041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 2101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 2161 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 2221 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc 2281 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 2341 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc 2401 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat 2461 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc 2521 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga 2581 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat 2641 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac 2701 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga 2761 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca 2821 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc 2881 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac 2941 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg 3001 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa 3061 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg 3121 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg 3181 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa 3241 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat 3301 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt 3361 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg 3421 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc 3481 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga 3541 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc 3601 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt 3661 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa 3721 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acaggctagc ctctaggcgc 3781 cggaattgaa gatctccgcg gttaattaaa atgatacggc gaccaccgag atctgtcttg 3841 atctaggagc tactcatcaa cggtcacatg tgagggccta tttcccatga ttccttcata 3901 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac 3961 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt 4021 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga 4081 tttcttggct ttatatatct tgtggaaagg acgaaacacc cgagacgtct accgcacgtc 4141 gtctcagttt aagagctatg ctgataactt cgtatagcat acattatacg aagttatttt 4201 ttttttttgc ggccgccaag agagcagaaa tagcaagtaa ctcattaggt aaataaggta 4261 gtccgtttag tgccgtagcc aattctcaga catttaatgg ctctaaacgt aacatttaca 4321 aattataact tcgtataatg tgtactatac gaagttatta attaattctg gttcgaatgt 4381 agaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 4441 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 4501 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 4561 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 4621 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 4681 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 4741 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 4801 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 4861 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggcc aagcctttgt 4921 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct 4981 ctgaggatta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg 5041 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg 5101 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca 5161 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag 5221 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct 5281 ctggttatgt gtgggagggc tgacctgcag gataacttcg tatagcatac attatacgaa 5341 gttatcagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc 5401 gagtcggtgc ttttttgttt tagcataact tcgtataatg tgtactatac gaagttatct 5461 agaggtaccc gttaagatcg gaagagcaca cgtctgaact ccagtcacaa aattatctcg 5521 tatgccgtct tatgcttgtt aattaacaat tgacgcgtaa ttctaccggc tagcgagagc 5581 acacacgaaa ggtaattcga tatcaagctt atcggtaatc aacctctgga ttacaaaatt 5641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 5761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 5821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 5881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 5941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 6061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 6181 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat 6241 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 6301 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 6361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 6421 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 6481 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga 6541 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag 6601 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta 6661 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg 6721 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 6781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 6841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 6901 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 6961 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 7021 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 7081 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 7141 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 7201 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 7261 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 7321 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 7381 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 7441 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 7501 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 7561 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 7621 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 7681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 7741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 7801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 7861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7921 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 7981 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt 8041 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg 8101 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt 8161 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg 8221 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca 8281 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg 8341 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt 8401 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg 8461 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg 8521 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 8581 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 8641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 8701 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 8761 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 8821 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 8881 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 8941 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 9001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 9061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 9121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 9181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 9241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 9301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 9361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 9421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 9481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 9541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 9601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 9661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 9721 tttgcaagca gcagattacg cg //