LOCUS       CRISPR-StAR4B_(L        9742 bp DNA     circular SYN 23-MAY-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   esther.uijttewaal
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             190..1050
                     /label=Amp
     misc_feature    1092..1130
                     /gene="Bacterial-Promoter prom"
                     /label=Bacterial-Promoter prom
     misc_feature    complement(1092..1120)
                     /direction=LEFT
                     /label=AMP promoter
                     /label=nonstandard type: promotor
     misc_feature    1523..1792
                     /label=CAG_enhancer
     misc_feature    2022..2203
                     /gene="RU5"
                     /label=RU5
     misc_signal     2313..2357
                     /label=psi
     misc_feature    2358..2710
                     /label=Gag
                     /note="Gag"
     misc_feature    2772..2787
                     /label=SV40_int
     misc_feature    2861..3159
                     /label=RRE
     misc_feature    3628..3751
                     /label=cPPT
                     /label=cPPT; cPPT/CTS
     misc_feature    3771..3781
                     /label=Psi+
     misc_feature    3872..4112
                     /label=U6 promoter
     misc_feature    4148..4163
                     /label=Tracr
     misc_recomb     4164..4197
                     /label=LoxP
     misc_feature    4198..4208
                     /label=stop
     misc_feature    4325..4365
                     /label=lox5171+PacI site
     misc_feature    4325..4357
                     /label=Lox5171
     misc_feature    4392..4891
                     /label=mouse pgk
     CDS             4905..5303
                     /codon_start=1
                     /label=blast
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_recomb     5312..5345
                     /label=LoxP
                     /label=LoxP(1)
     misc_feature    5346..5421
                     /label=Tracr + stop
     misc_feature    5425..5463
                     /label=lox5171+XbaI site
     misc_feature    5425..5457
                     /label=Lox5171
     misc_binding    5617..6205
                     /label=WPRE
     misc_feature    6393..6408
                     /label=cPPT
     misc_feature    6409..6462
                     /label=delta U3
     LTR             6676..6911
                     /label=3' LTR SIN
     CDS             6918..7149
                     /label=bGH polyA
     misc_feature    7229..7535
                     /label=f1 Origin
     promoter        7667..7935
                     /label=SV40_promoter
     gene            8097..8468
                     /gene="bleo"
                     /label=bleo
     terminator      8604..8735
                     /label=SV40_PA_terminator
     rep_origin      complement(9120..9340)
                     /direction=LEFT
                     /label=PMB1
ORIGIN
        1 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
       61 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
      361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
      421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
      481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     1201 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1261 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     1321 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     1381 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgcg cgttgacatt gattattgac
     1441 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     1501 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     1561 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     1621 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     1681 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     1741 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     1801 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     1861 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     1921 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     1981 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     2041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2161 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2221 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2281 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2341 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2401 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2461 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     2521 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     2581 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     2641 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     2701 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     2761 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     2821 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2881 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2941 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3001 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3061 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3121 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3181 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3241 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3301 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3361 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3421 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3481 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     3541 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     3601 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3661 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     3721 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acaggctagc ctctaggcgc
     3781 cggaattgaa gatctccgcg gttaattaaa atgatacggc gaccaccgag atctgtcttg
     3841 atctaggagc tactcatcaa cggtcacatg tgagggccta tttcccatga ttccttcata
     3901 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     3961 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     4021 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     4081 tttcttggct ttatatatct tgtggaaagg acgaaacacc cgagacgtct accgcacgtc
     4141 gtctcagttt aagagctatg ctgataactt cgtatagcat acattatacg aagttatttt
     4201 ttttttttgc ggccgccaag agagcagaaa tagcaagtaa ctcattaggt aaataaggta
     4261 gtccgtttag tgccgtagcc aattctcaga catttaatgg ctctaaacgt aacatttaca
     4321 aattataact tcgtataatg tgtactatac gaagttatta attaattctg gttcgaatgt
     4381 agaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     4441 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     4501 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     4561 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     4621 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     4681 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     4741 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     4801 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     4861 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggcc aagcctttgt
     4921 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     4981 ctgaggatta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     5041 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     5101 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     5161 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     5221 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     5281 ctggttatgt gtgggagggc tgacctgcag gataacttcg tatagcatac attatacgaa
     5341 gttatcagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     5401 gagtcggtgc ttttttgttt tagcataact tcgtataatg tgtactatac gaagttatct
     5461 agaggtaccc gttaagatcg gaagagcaca cgtctgaact ccagtcacaa aattatctcg
     5521 tatgccgtct tatgcttgtt aattaacaat tgacgcgtaa ttctaccggc tagcgagagc
     5581 acacacgaaa ggtaattcga tatcaagctt atcggtaatc aacctctgga ttacaaaatt
     5641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6181 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     6241 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     6301 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     6361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6421 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     6481 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     6541 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     6601 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     6661 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     6721 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6901 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6961 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     7021 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     7081 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     7141 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     7201 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7261 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7321 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7381 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7441 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7501 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7561 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7621 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7921 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7981 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     8041 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     8101 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     8161 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     8221 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     8281 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     8341 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     8401 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     8461 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8521 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8581 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     8641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8701 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     8761 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8821 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8881 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8941 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9721 tttgcaagca gcagattacg cg
//
