LOCUS       CRISPR-StAR4GN_(       10944 bp DNA     circular SYN 23-MAY-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   esther.uijttewaal
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             190..1050
                     /label=Amp
     misc_feature    1092..1130
                     /gene="Bacterial-Promoter prom"
                     /label=Bacterial-Promoter prom
     misc_feature    complement(1092..1120)
                     /direction=LEFT
                     /label=AMP promoter
                     /label=nonstandard type: promotor
     misc_feature    1523..1792
                     /label=CAG_enhancer
     misc_feature    2022..2203
                     /gene="RU5"
                     /label=RU5
     misc_signal     2313..2357
                     /label=psi
     misc_feature    2358..2710
                     /label=Gag
                     /note="Gag"
     misc_feature    2772..2787
                     /label=SV40_int
     misc_feature    2861..3159
                     /label=RRE
     misc_feature    3628..3751
                     /label=cPPT
                     /label=cPPT; cPPT/CTS
     misc_feature    3771..3781
                     /label=Psi+
     misc_feature    3872..4112
                     /label=U6 promoter
     misc_feature    4148..4163
                     /label=Tracr
     misc_recomb     4164..4197
                     /label=LoxP
     misc_feature    4198..4208
                     /label=stop
     misc_feature    4325..4365
                     /label=lox5171+PacI site
     misc_feature    4325..4357
                     /label=Lox5171
     misc_feature    4397..4906
                     /label=mouse pgknoBSMBi
                     /label=nonstandard type: promotor
     CDS             4927..5643
                     /label=GFP
     sig_peptide     5644..5709
                     /label=P2A
     gene            5710..6503
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR
     misc_recomb     6514..6547
                     /label=LoxP(1)
     misc_feature    6548..6623
                     /label=Tracr + stop
     misc_feature    6627..6665
                     /label=lox5171+XbaI site
     misc_feature    6627..6659
                     /label=Lox5171
     misc_binding    6819..7407
                     /label=WPRE
     misc_feature    7595..7610
                     /label=cPPT
     misc_feature    7611..7664
                     /label=delta U3
     LTR             7878..8113
                     /label=3' LTR SIN
     CDS             8120..8351
                     /label=bGH polyA
     misc_feature    8431..8737
                     /label=f1 Origin
     promoter        8869..9137
                     /label=SV40_promoter
     gene            9299..9670
                     /gene="bleo"
                     /label=bleo
     terminator      9806..9937
                     /label=SV40_PA_terminator
     rep_origin      complement(10322..10542)
                     /direction=LEFT
                     /label=PMB1
ORIGIN
        1 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
       61 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
      361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
      421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
      481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     1201 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1261 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     1321 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     1381 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgcg cgttgacatt gattattgac
     1441 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     1501 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     1561 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     1621 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     1681 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     1741 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     1801 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     1861 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     1921 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     1981 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     2041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2161 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2221 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2281 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2341 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2401 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2461 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     2521 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     2581 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     2641 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     2701 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     2761 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     2821 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2881 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2941 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3001 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3061 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3121 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3181 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3241 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3301 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3361 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3421 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3481 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     3541 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     3601 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3661 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     3721 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acaggctagc ctctaggcgc
     3781 cggaattgaa gatctccgcg gttaattaaa atgatacggc gaccaccgag atctgtcttg
     3841 atctaggagc tactcatcaa cggtcacatg tgagggccta tttcccatga ttccttcata
     3901 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     3961 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     4021 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     4081 tttcttggct ttatatatct tgtggaaagg acgaaacacc cgagacgtct accgcacgtc
     4141 gtctcagttt aagagctatg ctgataactt cgtatagcat acattatacg aagttatttt
     4201 ttttttttgc ggccgccaag agagcagaaa tagcaagtaa ctcattaggt aaataaggta
     4261 gtccgtttag tgccgtagcc aattctcaga catttaatgg ctctaaacgt aacatttaca
     4321 aattataact tcgtataatg tgtactatac gaagttatta attaattctg gttcgaatgt
     4381 agaattgtag cgatctaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     4441 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     4501 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     4561 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     4621 aggacgtgac aaatggaagt agcacctctc actagtctcg tgcagatgga cagcaccgct
     4681 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     4741 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     4801 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     4861 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccagaat
     4921 tccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4981 ctggacggcg acgtaaacgg ccacaagttc agcgtgtctg gcgagggcga gggcgatgcc
     5041 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5101 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5161 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5221 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5281 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5341 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5401 aagaacggca tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5461 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5521 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa acgcgatcac
     5581 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5641 aagggcagcg gcgccaccaa cttcagcctg ctgaagcagg ccggcgacgt ggaggagaac
     5701 cccggcccca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5761 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5821 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5881 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5941 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6001 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6061 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6121 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6181 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6241 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6301 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6361 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6421 ggccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6481 cgccttcttg acgagttctt ctgaacctgc aggataactt cgtatagcat acattatacg
     6541 aagttatcag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
     6601 ccgagtcggt gcttttttgt tttagcataa cttcgtataa tgtgtactat acgaagttat
     6661 ctagaggtac ccgttaagat cggaagagca cacgtctgaa ctccagtcac aaaattatct
     6721 cgtatgccgt cttatgcttg ttaattaaca attgacgcgt aattctaccg gctagcgaga
     6781 gcacacacga aaggtaattc gatatcaagc ttatcggtaa tcaacctctg gattacaaaa
     6841 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6901 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6961 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7021 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7081 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7141 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7201 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7261 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7321 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7381 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     7441 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7501 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7561 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7621 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7681 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7741 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7801 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7861 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7921 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7981 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8041 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8101 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     8161 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8221 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8281 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8341 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     8401 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8461 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8521 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8581 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8641 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8701 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8761 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8821 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8881 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8941 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9001 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9061 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9121 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9181 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     9241 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     9301 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     9361 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     9421 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     9481 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     9541 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     9601 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     9661 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     9721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10921 tgtttgcaag cagcagatta cgcg
//
