LOCUS       pLV-DualStAR-v.5       14076 bp DNA     circular SYN 24-MAY-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 14076)
  AUTHORS   VBC
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14076
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     CDS             complement(join(190..981,982..1050))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  190 ..  981 / #ccffcc
                        2:  982 .. 1050 / #ccffcc / signal sequence
                     Cleavage site after base 981"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1051..1155)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     enhancer        1425..1804
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        1806..2004
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             2022..2202
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2249..2374
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    2867..3100
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             3285..3329
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3627..3744
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        3872..4112
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    4148..4163
                     /label=Tracr
                     /note="color: #a6acb3"
     protein_bind    4164..4197
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    4198..4208
                     /label=stop
                     /note="color: #ff0000"
     misc_feature    4397..4496
                     /label=nonsense sequence to add
                     /note="color: #a6acb3"
     promoter        join(4525..4596,4597..5702)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="This forward directional feature has 2 segments:
                        1: 4525 .. 4596 / #ffffff
                        2: 4597 .. 5702 / #ffffff"
     intron          4756..5693
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     CDS             5737..6435
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /note="color: #3366ff"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     protein_bind    complement(6436..6469)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTACACAT). lox5171 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
                     /note="color: #31849b; direction: LEFT"
     CDS             6472..6528
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 6525"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6529..7320
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWAGRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7321..7374
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 7371"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7375..7434
                     /codon_start=1
                     /label=Gap43
                     /note="Mouse Gap43 first 20 a.a. for membrane localization"
                     /note="color: #ff9900"
                     /translation="MLCCMRRTKQVEKNDEDQKI"
     regulatory      7453..7462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             7459..8406
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /note="color: #a0003c"
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     protein_bind    8416..8449
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    8450..8525
                     /label=Tracr + stop
                     /note="color: #a6acb3"
     promoter        8532..9031
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             9045..9746
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     protein_bind    complement(9756..9789)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTACACAT). lox5171 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
                     /note="color: #31849b; direction: LEFT"
     misc_feature    9951..10539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(10422..10433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 10421"
                     /translation="IEGR"
     primer_bind     complement(10542..10558)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             11064..11244
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    11276..11500
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      11546..11974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        11988..12317
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      12168..12303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        12365..12412
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             12431..12805
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12935..13056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(13105..13121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    13129..13145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(13153..13159,13160..13177,13178..13183))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 13153 .. 13159 / #ffffff / -10
                        2: 13160 .. 13177 / #ffffff
                        3: 13178 .. 13183 / #ffffff / -35"
     protein_bind    13198..13219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(join(13507..14076,1..19))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
       61 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
      361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
      421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
      481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     1201 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1261 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     1321 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     1381 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgcg cgttgacatt gattattgac
     1441 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     1501 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     1561 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     1621 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     1681 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     1741 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     1801 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     1861 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     1921 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     1981 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     2041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2161 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2221 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2281 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2341 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2401 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2461 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     2521 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     2581 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     2641 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     2701 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     2761 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     2821 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2881 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2941 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3001 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3061 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3121 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3181 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3241 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3301 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3361 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3421 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3481 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     3541 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     3601 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3661 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     3721 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acaggctagc ctctaggcgc
     3781 cggaattgaa gatctccgcg gttaattaaa atgatacggc gaccaccgag atctgtcttg
     3841 atctaggagc tactcatcaa cggtcacatg tgagggccta tttcccatga ttccttcata
     3901 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     3961 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     4021 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     4081 tttcttggct ttatatatct tgtggaaagg acgaaacacc cgagacgtct accgcacgtc
     4141 gtctcagttt aagagctatg ctgataactt cgtatagcat acattatacg aagttatttt
     4201 ttttttttgc tcgagtagaa gttaacggtc atcattgaat ctggaggaga gctaagttaa
     4261 taaggtcggt gatggttggt tagatttatg tcgtagtcac atccacgcgg ccgctgtatt
     4321 cacgactgcg tgtctgaact atcatctggg actgcttaat cggatgtgtc tcatccaact
     4381 gagttggggg ctactgagag cagaaatagc aagtaactca ttaggtaaat aaggtagtcc
     4441 gtttagtgcc gtagccaatt ctcagacatt taatggctct aaacgtaaca tttacaaatt
     4501 ttcgaagctc cggtgcccgt cagtggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4561 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     4621 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4681 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4741 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     4801 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     4861 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     4921 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     4981 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     5041 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     5101 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     5161 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     5221 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     5281 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     5341 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     5401 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     5461 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     5521 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     5581 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     5641 tggttcattc tcaagcctca gacagtggtt caaagttttt tcttccattt caggtgtcgt
     5701 gacgtacggc caccatgagc ggcctctgag gccaccatga gcgagctgat taaggagaac
     5761 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     5821 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     5881 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     5941 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     6001 acatgggaga gagtcaccac atacgaggac gggggcgtgc tgaccgctac ccaggacacc
     6061 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     6121 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga aacgctgtac
     6181 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     6241 catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     6301 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     6361 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     6421 gggcacaagc ttaatataac ttcgtataat gtgtactata cgaagttatc tgccaccaac
     6481 ttcagcctgc tgaagcaggc cggcgacgtg gaggagaacc ccggccccat gattgaacaa
     6541 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6601 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6661 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6721 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6781 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6841 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6901 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6961 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     7021 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     7081 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     7141 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     7201 acccgtgata ttgctgaaga gcttggcggc gaatgggctg gccgcttcct cgtgctttac
     7261 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     7321 gagggcagag gcagtctgct gacatgcggt gacgtggaag agaatcccgg ccctatgctg
     7381 tgctgtatga gaagaaccaa acaggttgaa aagaatgatg aggaccaaaa gattgggatc
     7441 caccggccgg tcgccaccat ggtagcaggt catgcctctg gcagccccgc attcgggacc
     7501 gcctctcatt cgaattgcga acatgaagag atccacctcg ccggctcgat ccagccgcat
     7561 ggcgcgcttc tggtcgtcag cgaacatgat catcgcgtca tccaggccag cgccaacgcc
     7621 gcggaatttc tgaatctcgg aagcgtactc ggcgttccgc tcgccgagat cgacggcgat
     7681 ctgttgatca agatcctgcc gcatctcgat cccaccgccg aaggcatgcc ggtcgcggtg
     7741 cgctgccgga tcggcaatcc ctctacggag tactgcggtc tgatgcatcg gcctccggaa
     7801 ggcgggctga tcatcgaact cgaacgtgcc ggcccgtcga tcgatctgtc aggcacgctg
     7861 gcgccggcgc tggagcggat ccgcacggcg ggttcactgc gcgcgctgtg cgatgacacc
     7921 gtgctgctgt ttcagcagtg caccggctac gaccgggtga tggtgtatcg tttcgatgag
     7981 caaggccacg gcctggtatt ctccgagtgc catgtgcctg ggctcgaatc ctatttcggc
     8041 aaccgctatc cgtcgtcgct ggtcccgcag atggcgcggc agctgtacgt gcggcagcgc
     8101 gtccgcgtgc tggtcgacgt cacctatcag ccggtgccgc tggagccgcg gctgtcgccg
     8161 ctgaccgggc gcgatctcga catgtcgggc tgcttcctgc gctcgatgtc gccgatccat
     8221 ctgcagttcc tgaaggacat gggcgtgcgc gccaccctgg cggtgtcgct ggtggtcggc
     8281 ggcaagctgt ggggcctggt tgtctgtcac cattatctgc cgcgcttcat ccgtttcgag
     8341 ctgcgggcga tctgcaaacg gctcgccgaa aggatcgcga cgcggatcac cgcgcttgag
     8401 agctaaacct gcaggataac ttcgtatagc atacattata cgaagttatc agcatagcaa
     8461 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     8521 gttttagttg tgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     8581 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     8641 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     8701 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     8761 ggaagtagca cctctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     8821 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     8881 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     8941 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     9001 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatgttg agcaagggcg
     9061 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
     9121 atgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     9181 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     9241 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
     9301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
     9361 tgaccgtgac ccaggactcc tccctccagg acggcacgct gatctacaag gtgaagatgc
     9421 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagacaatg ggctgggagg
     9481 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
     9541 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
     9601 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
     9661 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
     9721 tgtacggcat ggacgagctg tacaagtgag gatccataac ttcgtataat gtgtactata
     9781 cgaagttata gtctagaggt acccgttaag atcggaagag cacacgtctg aactccagtc
     9841 acaaaattat ctcgtatgcc gtcttatgct tgttaattaa caattgacgc gtaattctac
     9901 cggctagcga gagcacacac gaaaggtaat tcgatatcaa gcttatcggt aatcaacctc
     9961 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
    10021 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
    10081 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
    10141 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
    10201 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
    10261 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
    10321 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
    10381 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
    10441 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
    10501 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
    10561 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
    10621 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
    10681 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    10741 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
    10801 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
    10861 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
    10921 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
    10981 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
    11041 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
    11101 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    11161 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    11221 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
    11281 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    11341 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    11401 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    11461 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    11521 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    11581 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    11641 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    11701 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    11761 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    11821 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    11881 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    11941 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
    12001 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    12061 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    12121 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    12181 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    12241 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    12301 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    12361 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
    12421 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
    12481 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
    12541 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    12601 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    12661 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    12721 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    12781 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    12841 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    12901 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    12961 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    13021 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    13081 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    13141 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    13201 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    13261 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    13321 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    13381 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    13441 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    13501 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    13561 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    13621 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    13681 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    13741 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    13801 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    13861 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    13921 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    13981 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    14041 cggtggtttt tttgtttgca agcagcagat tacgcg
//
