LOCUS       CRISPR-StAR1_(Re        8114 bp DNA     circular SYN 23-MAY-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
COMMENT     LOCUS LSL 7103 bp ds-DNA circular 10-NOV-2016 DEFINITION martin 
            aichinger zuber lab. ACCESSION urn.local...10-33pqa34 KEYWORDS . 
            SOURCE ORGANISM . COMMENT p76 + g loxP STOP COMMENT 
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..8114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..684
                     /label=5'LTR
     misc_feature    690..1568
                     /label=Psi+
     misc_feature    1666..1906
                     /label=U6 promoter
     misc_feature    1939..1955
                     /label=Tracr
     misc_recomb     1956..1989
                     /label=LoxP
     misc_feature    1990..2000
                     /label=stop
     misc_feature    2017..2057
                     /label=lox5171+PacI site
     misc_feature    2017..2049
                     /label=Lox5171
     misc_feature    2081..2580
                     /label=mouse pgk
     CDS             2594..2992
                     /codon_start=1
                     /label=blast
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_recomb     3001..3034
                     /label=LoxP(1)
     misc_feature    3035..3110
                     /label=Tracr + stop
     misc_feature    3111..3189
                     /label=not necessary
     misc_feature    3190..3228
                     /label=lox5171+XbaI site
     misc_feature    3190..3222
                     /label=Lox5171
     misc_feature    3266..3292
                     /label=stochasticConstruct-R
                     /note="primer"
     misc_feature    3333..3832
                     /label=mouse pgk
     CDS             3846..4649
                     /label=Neo
     LTR             4767..5387
                     /label=LTR-SIN
     CDS             complement(6892..7752)
                     /label=AMP
     misc_feature    complement(7794..7822)
                     /label=AMP promoter
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
      541 cggcgcgcca gtctcccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
      601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
      721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
      781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
      841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1561 taggcgccgg aattgaaggt cgtagatctc cgcggttaat taaaatgata cggcgaccac
     1621 cgagatctgt cttgatctag gagctactca tcaacggtca catgtgaggg cctatttccc
     1681 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     1741 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     1801 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     1861 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct
     1921 tcgctcgaga gaagacctgt ttaagagcta tgctgataac ttcgtatagc atacattata
     1981 cgaagttatt tttttttttt gcggccgccc agaattataa cttcgtataa tgtgtactat
     2041 acgaagttat taattaattc tggcctgcag gaattctacc gggtagggga ggcgcttttc
     2101 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     2161 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     2221 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     2281 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     2341 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     2401 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     2461 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     2521 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     2581 acctgcagcc aatatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     2641 aacggctaca atcaacagca tccccatctc tgaggattac agcgtcgcca gcgcagctct
     2701 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     2761 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     2821 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     2881 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     2941 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct gacctgcagg
     3001 ataacttcgt atagcataca ttatacgaag ttatcagcat agcaagttta aataaggcta
     3061 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt agagctagaa
     3121 atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct gcagacaaat
     3181 ggctctagca taacttcgta taatgtgtac tatacgaagt tatctagagg tacccgttaa
     3241 gatcggaaga gcacacgtct gaactccagt cacaaaatta tctcgtatgc cgtcttatgc
     3301 ttgttaatta acaattgacg cgtaattcta ccgggtaggg gaggcgcttt tcccaaggca
     3361 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     3421 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     3481 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     3541 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     3601 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     3661 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     3721 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     3781 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     3841 ccaatatggg atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3901 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3961 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4021 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4081 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4141 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4201 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4261 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4321 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4381 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4441 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4501 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4561 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4621 tctatcgcct tcttgacgag ttcttctgag gggatccgtc gacctgcagc caagcttatc
     4681 gataccgtcg agatttaatt aagatatcat cagtggtcca ggctctagtt ttgactcaac
     4741 aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag attttattta
     4801 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     4861 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     4921 ggtcaggaac agatggaaca gggtcgaccc tagagaacca tcagatgttt ccagggtgcc
     4981 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     5041 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     5101 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     5161 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     5221 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     5281 gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc ggtgatgacg
     5341 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     5401 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     5461 ccatgaccca gtcacgtagc gatagcggag tgtagatccg gctgtggaat gtgtgtcagt
     5521 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5581 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5641 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5701 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5761 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5821 gaggcctagg cttttgcaaa aagcttactg gcttaactat gcggcatcag agcagattgt
     5881 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     5941 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6001 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6061 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6121 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6181 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6241 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6301 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6361 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6421 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6481 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6541 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6601 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6661 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6721 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6781 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6841 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6901 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6961 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7021 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7081 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7141 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7201 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7261 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7321 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7381 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7441 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7501 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7561 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7621 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7681 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7741 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7801 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7861 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     7921 taaaaatagg cgtatcacga ggccctttcg tctacaagaa ttagcttggc cattgcatac
     7981 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg
     8041 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     8101 cccatatatg gagt
//
