LOCUS CRISPR-StAR2_(Re 8263 bp DNA circular SYN 23-MAY-2024 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8263) AUTHORS esther.uijttewaal TITLE Direct Submission JOURNAL Exported May 23, 2024 from SnapGene 7.0.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8263 /mol_type="other DNA" /organism="recombinant plasmid" LTR 1..684 /label=5'LTR misc_feature 690..1568 /label=Psi+ misc_feature 1666..1906 /label=U6 promoter misc_feature 1939..1955 /label=Tracr misc_recomb 1956..1989 /label=LoxP misc_feature 1990..2000 /label=stop misc_feature 2117..2157 /label=lox5171+PacI site misc_feature 2117..2149 /label=Lox5171 misc_recomb 2174..2207 /label=LoxP misc_feature 2230..2729 /label=mouse pgk CDS 2743..3141 /codon_start=1 /label=blast /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_recomb 3150..3183 /label=LoxP /label=LoxP(1) misc_feature 3184..3259 /label=Tracr + stop misc_feature 3339..3377 /label=lox5171+XbaI site misc_feature 3339..3371 /label=Lox5171 misc_feature 3482..3981 /label=mouse pgk CDS 3995..4798 /label=Neo LTR 4916..5536 /label=LTR-SIN CDS complement(7041..7901) /label=AMP misc_feature complement(7943..7971) /label=AMP promoter ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc 241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact 541 cggcgcgcca gtctcccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct 601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga 661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg 721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt 781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact 841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc 901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt 961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac 1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc 1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt 1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag 1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct 1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc 1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag 1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca 1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc 1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc 1561 taggcgccgg aattgaaggt cgtagatctc cgcggttaat taaaatgata cggcgaccac 1621 cgagatctgt cttgatctag gagctactca tcaacggtca catgtgaggg cctatttccc 1681 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat 1741 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt 1801 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact 1861 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct 1921 tcgctcgaga gaagacctgt ttaagagcta tgctgataac ttcgtatagc atacattata 1981 cgaagttatt tttttttttt gcggccgcca agagagcaga aatagcaagt aactcattag 2041 gtaaataagg tagtccgttt agtgccgtag ccaattctca gacatttaat ggctctaaac 2101 gtaacattta caaattataa cttcgtataa tgtgtactat acgaagttat taattaattc 2161 tggttcgaac ctgataactt cgtatagcat acattatacg aagttatcat tcgaatgtag 2221 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag 2281 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca 2341 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc 2401 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg 2461 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg 2521 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc 2581 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc 2641 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt 2701 tctcctcttc ctcatctccg ggcctttcga cctgcagcca atatggccaa gcctttgtct 2761 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct 2821 gaggattaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc 2881 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct 2941 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc 3001 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc 3061 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct 3121 ggttatgtgt gggagggctg acctgcagga taacttcgta tagcatacat tatacgaagt 3181 tatcagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga 3241 gtcggtgctt ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt 3301 tagcgcgtgc gccaattctg cagacaaatg gctctagcat aacttcgtat aatgtgtact 3361 atacgaagtt atctagaggt acccgttaag atcggaagag cacacgtctg aactccagtc 3421 acaaaattat ctcgtatgcc gtcttatgct tgttaattaa caattgacgc gtaattctac 3481 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg 3541 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg 3601 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc 3661 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca 3721 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt 3781 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg 3841 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc 3901 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct 3961 tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg aacaagatgg 4021 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 4081 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 4141 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 4201 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4261 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4321 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4381 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4441 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4501 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4561 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4621 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4681 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4741 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg 4801 ggatccgtcg acctgcagcc aagcttatcg ataccgtcga gatttaatta agatatcatc 4861 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga 4921 gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc 4981 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac 5041 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ggtcgaccct 5101 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt 5161 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca 5221 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg 5281 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc 5341 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg 5401 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc 5461 tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 5521 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 5581 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 5641 gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 5701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 5761 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 5821 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 5881 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 5941 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactgg 6001 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 6061 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact 6121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 6181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 6241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 6301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 6361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 6421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 6481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 6541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 6601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 6661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 6721 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 6781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 6841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 6901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 6961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 7021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 7081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 7141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 7201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 7261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 7321 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg 7381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 7441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 7501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 7561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 7621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 7681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 7741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 7801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 7861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 7921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 7981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 8041 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 8101 ctacaagaat tagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat 8161 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag 8221 taatcaatta cggggtcatt agttcatagc ccatatatgg agt //