LOCUS       CRISPR-StAR2_(Re        8263 bp DNA     circular SYN 23-MAY-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   esther.uijttewaal
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8263
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             1..684
                     /label=5'LTR
     misc_feature    690..1568
                     /label=Psi+
     misc_feature    1666..1906
                     /label=U6 promoter
     misc_feature    1939..1955
                     /label=Tracr
     misc_recomb     1956..1989
                     /label=LoxP
     misc_feature    1990..2000
                     /label=stop
     misc_feature    2117..2157
                     /label=lox5171+PacI site
     misc_feature    2117..2149
                     /label=Lox5171
     misc_recomb     2174..2207
                     /label=LoxP
     misc_feature    2230..2729
                     /label=mouse pgk
     CDS             2743..3141
                     /codon_start=1
                     /label=blast
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_recomb     3150..3183
                     /label=LoxP
                     /label=LoxP(1)
     misc_feature    3184..3259
                     /label=Tracr + stop
     misc_feature    3339..3377
                     /label=lox5171+XbaI site
     misc_feature    3339..3371
                     /label=Lox5171
     misc_feature    3482..3981
                     /label=mouse pgk
     CDS             3995..4798
                     /label=Neo
     LTR             4916..5536
                     /label=LTR-SIN
     CDS             complement(7041..7901)
                     /label=AMP
     misc_feature    complement(7943..7971)
                     /label=AMP promoter
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
      541 cggcgcgcca gtctcccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
      601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
      721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
      781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
      841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1561 taggcgccgg aattgaaggt cgtagatctc cgcggttaat taaaatgata cggcgaccac
     1621 cgagatctgt cttgatctag gagctactca tcaacggtca catgtgaggg cctatttccc
     1681 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     1741 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     1801 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     1861 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct
     1921 tcgctcgaga gaagacctgt ttaagagcta tgctgataac ttcgtatagc atacattata
     1981 cgaagttatt tttttttttt gcggccgcca agagagcaga aatagcaagt aactcattag
     2041 gtaaataagg tagtccgttt agtgccgtag ccaattctca gacatttaat ggctctaaac
     2101 gtaacattta caaattataa cttcgtataa tgtgtactat acgaagttat taattaattc
     2161 tggttcgaac ctgataactt cgtatagcat acattatacg aagttatcat tcgaatgtag
     2221 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     2281 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     2341 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     2401 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     2461 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     2521 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     2581 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     2641 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     2701 tctcctcttc ctcatctccg ggcctttcga cctgcagcca atatggccaa gcctttgtct
     2761 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     2821 gaggattaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     2881 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     2941 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     3001 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     3061 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     3121 ggttatgtgt gggagggctg acctgcagga taacttcgta tagcatacat tatacgaagt
     3181 tatcagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     3241 gtcggtgctt ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt
     3301 tagcgcgtgc gccaattctg cagacaaatg gctctagcat aacttcgtat aatgtgtact
     3361 atacgaagtt atctagaggt acccgttaag atcggaagag cacacgtctg aactccagtc
     3421 acaaaattat ctcgtatgcc gtcttatgct tgttaattaa caattgacgc gtaattctac
     3481 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     3541 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     3601 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     3661 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     3721 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     3781 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     3841 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     3901 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     3961 tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg aacaagatgg
     4021 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4081 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4141 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     4201 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4261 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     4321 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     4381 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     4441 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4501 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     4561 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     4621 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4681 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4741 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg
     4801 ggatccgtcg acctgcagcc aagcttatcg ataccgtcga gatttaatta agatatcatc
     4861 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga
     4921 gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     4981 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
     5041 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ggtcgaccct
     5101 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     5161 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     5221 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     5281 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     5341 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
     5401 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
     5461 tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     5521 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     5581 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     5641 gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5761 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5821 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5881 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5941 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactgg
     6001 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     6061 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     6121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6721 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7321 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     7381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     7681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     8041 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     8101 ctacaagaat tagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat
     8161 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag
     8221 taatcaatta cggggtcatt agttcatagc ccatatatgg agt
//
