LOCUS       B2M-CD47_SEED           4712 bp ds-DNA     circular     29-JUN-2024
DEFINITION  .
KEYWORDS    "Made by:Joe" "Vector:AAV6" "Locus:hB2M" "Architecture" "Antigen
            target" "Co-Stim(s)" "Tag:Myc-tag" "Binder linker" "Fluorescent
            protein" "Bacterial resistance:Amp" "Status:Glycerol stock,
            Maxi-Prep" "Notes"
FEATURES             Location/Qualifiers
     misc_feature    complement(1..141)
                     /label="L ITR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            163..472
                     /label="B2M Intron LHA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(467..469)
                     /label="PAM for G576: B2M intron guide 6"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(470..472)
                     /label="G576: B2M intron guide 6"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    473..532
                     /label="Polypyrimidine/branch point"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     https://www.... 473..536
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    534..536
                     /label="splice acceptor"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    537..538
                     /label="2 Extra bases from downstream exon to keep in frame"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    539..604
                     /label="P2A*"
                     /ApEinfo_revcolor="#c0640f"
                     /ApEinfo_fwdcolor="#c0640f"
     CDS             539..604
                     /label="Translation 539-604"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     misc_feature    605..643
                     /label="Signal Peptide"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    605..1573
                     /label="SEED Cargo"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    605..1573
                     /label="CD47"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             605..1573
                     /label="Translation 605-1573"
                     /translation="MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE"
     misc_feature    644..1027
                     /label="CD47 ECD"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1574..1639
                     /label="P2A"
                     /ApEinfo_revcolor="#c0721f"
                     /ApEinfo_fwdcolor="#c0721f"
     CDS             1574..1639
                     /label="Translation 1574-1639"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     gene            1640..1640
                     /label="Extra base to keep in frame downstream from Exon 1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     exon            1640..1640
                     /label="B2M-001 Exon 1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            1641..1650
                     /label="Splice Donor (from after B2M exon 1 before)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1651..1667)
                     /label="G576: B2M intron guide 6"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            1651..1960
                     /label="B2M Intron RHA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1975..2115)
                     /label="R ITR"
                     /ApEinfo_revcolor="#2046c0"
                     /ApEinfo_fwdcolor="#2046c0"
     rep_origin      complement(2157..2785)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    complement(2936..3796)
                     /label="AMPr"
                     /ApEinfo_revcolor="#be610d"
                     /ApEinfo_fwdcolor="#be610d"
     Promoter        complement(3838..3866)
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      complement(4183..4638)
                     /label="M13 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      4193..4633
                     /label="F1 ori"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCT CGGTCCGcta gcTTTTATAC ACATATATTT
      181 agtgtcaatc tgtatattta gtagcactaa cacttctctt cattttcaat gaaaaatata
      241 gagtttataa tattttcttc ccacttcccc atggatggtc tAGTCATGCC TCTCATTTTG
      301 GAAAGTACTG TTTCTGAAAC ATTAGGCAAT ATATTCCCAA CCTGGCTAGT TTACAGCAAT
      361 CACCTGTGGA TGCTAATTAA AACGCAAATC CCACTGTCAC ATGCATTACT CCATTTGATC
      421 ATAATGGAAA gtatgttctg tcccatttgc catagtcctc acctatccct gtctcttgcg
      481 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacagGTGG
      541 AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA GACGTGGAGG AGAACCCTGG
      601 ACCCATGTGG CCTTTAGTAG CAGCCCTTCT GCTCGGAAGC GCCTGCTGCG GATCAGCGCA
      661 ACTGCTGTTT AACAAAACCA AATCCGTGGA ATTCACATTC TGTAACGATA CTGTCGTGAT
      721 CCCTTGCTTT GTGACTAATA TGGAAGCACA GAATACGACC GAAGTGTATG TGAAGTGGAA
      781 ATTCAAGGGA CGGGATATAT ATACATTCGA CGGCGCCCTA AATAAGAGCA CCGTGCCAAC
      841 CGACTTTTCT AGCGCCAAGA TTGAGGTATC TCAGCTTCTC AAGGGGGATG CCAGTCTGAA
      901 AATGGACAAA TCCGATGCCG TGTCTCATAC AGGTAACTAC ACCTGTGAGG TCACCGAACT
      961 GACACGCGAG GGTGAAACAA TCATCGAACT CAAGTACAGA GTCGTCAGCT GGTTCTCCCC
     1021 AAATGAGAAC ATATTAATTG TGATTTTTCC TATCTTCGCC ATCCTTCTTT TCTGGGGTCA
     1081 GTTTGGAATT AAAACGCTAA AGTATCGATC GGGCGGGATG GACGAGAAGA CTATTGCTTT
     1141 ACTGGTTGCA GGCCTGGTGA TAACCGTTAT TGTCATAGTG GGCGCGATTC TCTTTGTCCC
     1201 CGGGGAGTAC TCATTGAAGA ATGCCACTGG CCTGGGGTTG ATTGTCACAT CAACTGGAAT
     1261 CCTGATTCTG TTGCACTATT ACGTGTTCAG CACCGCAATC GGGTTGACGA GTTTCGTGAT
     1321 CGCAATCCTC GTGATACAGG TTATCGCCTA CATCCTGGCG GTTGTGGGGC TCTCTCTGTG
     1381 CATCGCTGCT TGTATTCCCA TGCACGGACC CCTACTGATT TCAGGCCTGT CCATCCTCGC
     1441 TCTCGCCCAG CTTCTGGGCT TGGTATACAT GAAATTTGTA GCTAGTAACC AGAAGACTAT
     1501 CCAACCACCC AGGAAAGCTG TTGAGGAGCC GCTGAATGCT TTTAAAGAAT CCAAGGGCAT
     1561 GATGAACGAC GAGGGATCTG GAGCAACAAA CTTCTCACTA CTCAAACAAG CAGGTGACGT
     1621 GGAGGAGAAT CCCGGCCCCC GTGAGTCTCT tgtattttat cgggtccaac tcaaccattt
     1681 aaggtatttg ccagctcttg tatgcattta ggtttTGTTT CTTTGTTTTT TAGCTCATGA
     1741 AATTAGGTAC AAAGTCAGAG AGGGGTCTGG CATATAAAAC CTCAGCAGAA ATAAAGAGGT
     1801 TTTGTTGTTT GGTAAGAACA TACCTTGGGT Tggttgggca cggtggctcg tgcctgtaat
     1861 cccaacactt tgggaggcca aggcaggctg atcacttgaa gttgggagtt caagaccagc
     1921 ctggccaaca tggtgaaatc ccgtctctac tgaaaataca ACGCGTGCGG CCGCAGGAAC
     1981 CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC
     2041 GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC
     2101 GCAGCTGCCT GCAGGACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
     2161 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
     2221 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     2281 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
     2341 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
     2401 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
     2461 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
     2521 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
     2581 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC
     2641 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
     2701 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
     2761 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
     2821 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
     2881 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
     2941 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
     3001 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
     3061 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
     3121 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
     3181 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
     3241 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
     3301 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
     3361 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
     3421 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
     3481 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
     3541 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
     3601 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
     3661 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
     3721 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
     3781 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
     3841 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
     3901 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA
     3961 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG
     4021 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG
     4081 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA
     4141 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAAAATTGTA AACGTTAATA
     4201 TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG
     4261 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGCCCGA GATAGGGTTG AGTGTTGTTC
     4321 CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA
     4381 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CAAATCAAGT TTTTTGGGGT
     4441 CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC
     4501 GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA
     4561 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG
     4621 CGCCGCTACA GGGCGCGTAC TATGGTTGCT TTGACGTATG CGGTGTGAAA TACCGCACAG
     4681 ATGCGTAAGG AGAAAATACC GCATCAGGCG CC
//
LOCUS       CD4-CD8_SEED            5118 bp ds-DNA     circular     29-JUN-2024
DEFINITION  .
KEYWORDS    "Made by" "Vector" "Locus" "Architecture" "Antigen target"
            "Co-Stim(s)" "Tag" "Binder linker" "Fluorescent protein" "Bacterial
            resistance" "Status" "Notes"
FEATURES             Location/Qualifiers
     misc_feature    complement(1..141)
                     /label="Right ITR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            163..462
                     /label="CD4"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(457..459)
                     /label="PAM for G570: CD4 intron test 12"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(460..462)
                     /label="G570: CD4 intron test 12"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    463..522
                     /label="Polypyrimidine/branch point"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     https://www.... 463..526
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    524..526
                     /label="splice acceptor"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     gene            527..528
                     /label="Extra 2 bases to keep in frame"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    529..594
                     /label="P2A*"
                     /ApEinfo_revcolor="#c0640f"
                     /ApEinfo_fwdcolor="#c0640f"
     CDS             529..594
                     /label="Translation 529-594"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     exon            595..643
                     /label="CD8A-006 Exon 4"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    595..657
                     /label="SIGNAL PEPTIDE"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             595..1995
                     /label="Translation 595-1995"
                     /translation="MALPVTALLLPLALLLHAARPSQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCNHRNRRRVCKCPRPVVKSGDKPSLSARYVGSGATNFSLLKQAGDVEENPGPMRPRLWLLLAAQLTVLHGNSVLQQTPAYIKVQTNKMVMLSCEAKISLSNMRIYWLRQRQAPSSDSHHEFLALWDSAKGTIHGEEVEQEKIAVFRDASRFILNLTSVKPEDSGIYFCMIVGSPELTFGKGTQLSVVDFLPTTAQPTKKSTLKKRVCRLPRPETQKGPLCSPITLGLLVAGVLVLLVSLGVAIHLCCRRRRARLRFMKQFYK"
     exon            644..997
                     /label="CD8A-006 Exon 5"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     exon            998..1108
                     /label="CD8A-006 Exon 6"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            1109..1219
                     /label="CD8A-006 Exon 7"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            1220..1250
                     /label="CD8A-006 Exon 8"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            1251..1299
                     /label="CD8A-006 Exon 9"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1297..1299
                     /label="removed TAA (stop codon) after this to continue translation of P2A and CD8B"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1300..1365
                     /label="P2A"
                     /ApEinfo_revcolor="#c0721f"
                     /ApEinfo_fwdcolor="#c0721f"
     exon            1366..1408
                     /label="CD8B-001 Exon 1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            1366..1995
                     /label="CD8B"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            1409..1768
                     /label="CD8B-001 Exon 2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            1769..1858
                     /label="CD8B-001 Exon 3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     exon            1859..1948
                     /label="CD8B-001 Exon 4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            1949..1985
                     /label="CD8B-001 Exon 5"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            1986..1995
                     /label="CD8B-001 Exon 6"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1996..2061
                     /label="P2A**"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1996..2061
                     /label="Translation 1996-2061"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     misc_feature    2062..2062
                     /label="Extra Base - Maintain Reading Frame"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     gene            2062..2062
                     /label="CD4"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            2063..2068
                     /label="CD4 Exon5 Splice Donor"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            2069..2368
                     /label="CD4"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2383..2523)
                     /label="Left ITR"
                     /ApEinfo_revcolor="#2046c0"
                     /ApEinfo_fwdcolor="#2046c0"
     rep_origin      complement(2565..3193)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    complement(3345..4202)
                     /label="AMPr"
                     /ApEinfo_revcolor="#c08113"
                     /ApEinfo_fwdcolor="#c08113"
     Promoter        complement(4244..4272)
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      complement(4589..5044)
                     /label="M13 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      4599..5039
                     /label="F1 ori"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCT CGGTCCGcta gcATCCTCAG GGGGCTGATT
      181 GGCAGCCACC CCTCAGTGTG GTGGACATGG AGAAAGGAAA GGCTGGGGAA GGTAAGGATG
      241 CTAGAGGCCC GAGTCTCCTT TGGAGGCCCC AAAGGAGGAA TGTCAGGGAG CTTACTTTCT
      301 TTGTTGCCTC AGCTCCACAC CCCTACCAAG TTGGCAAATC CACTTACTCA GGGACACTAA
      361 CACCAGTAAG CCAACCCTGA TGATGTTCTA TGTTGTACCT CTGGACCTCT AAGCCAGGCC
      421 ACTGTGGGGA GACCAAGGTC CTACCCCAGA TCCTGTCCCC TGctcttgcg tttctgatag
      481 gcacctattg gtcttactga catccacttt gcctttctct ccacagCTGG AAGCGGAGCT
      541 ACTAACTTCA GCCTGCTGAA GCAGGCTGGA GACGTGGAGG AGAACCCTGG ACCCATGGCC
      601 TTACCAGTGA CCGCCTTGCT CCTGCCGCTG GCCTTGCTGC TCCACGCCGC CAGGCCGAGC
      661 CAGTTCCGGG TGTCGCCGCT GGATCGGACC TGGAACCTGG GCGAGACAGT GGAGCTGAAG
      721 TGCCAGGTGC TGCTGTCCAA CCCGACGTCG GGCTGCTCGT GGCTCTTCCA GCCGCGCGGC
      781 GCCGCCGCCA GTCCCACCTT CCTCCTATAC CTCTCCCAAA ACAAGCCCAA GGCGGCCGAG
      841 GGGCTGGACA CCCAGCGGTT CTCGGGCAAG AGGTTGGGGG ACACCTTCGT CCTCACCCTG
      901 AGCGACTTCC GCCGAGAGAA CGAGGGCTAC TATTTCTGCT CGGCCCTGAG CAACTCCATC
      961 ATGTACTTCA GCCACTTCGT GCCGGTCTTC CTGCCAGCGA AGCCCACCAC GACGCCAGCG
     1021 CCGCGACCAC CAACACCGGC GCCCACCATC GCGTCGCAGC CCCTGTCCCT GCGCCCAGAG
     1081 GCGTGCCGGC CAgcggcggg gggcgcagTG CACACGAGGG GGCTGGACTT CGCCTGTGAT
     1141 ATCTACATCT GGGCGCCCTT GGCCGGGACT TGTGGGGTCC TTCTCCTGTC ACTGGTTATC
     1201 ACCCTTTACT GCAACCACAG GAACCGAAGA CGTGTTTGCA AATGTCCCCG GCCTGTGGTC
     1261 AAATCGGGAG ACAAGCCCAG CCTTTCGGCG AGATACGTCG GATCTGGAGC AACAAACTTC
     1321 TCACTACTCA AACAAGCAGG TGACGTGGAG GAGAATCCCG GCCCcATGCG GCCGCGGCTG
     1381 TGGCTCCTCT TGGCCGCGCA GCTGACAGTT CTCCATGGCA ACTCAGTCCT CCAGCAGACC
     1441 CCTGCATACA TAAAGGTGCA AACCAACAAG ATGGTGATGC TGTCCTGCGA GGCTAAAATC
     1501 TCCCTCAGTA ACATGCGCAT CTACTGGCTG AGACAGCGCC AGGCACCGAG CAGTGACAGT
     1561 CACCACGAGT TCCTGGCCCT CTGGGATTCC GCAAAAGGGA CTATCCACGG TGAAGAGGTG
     1621 GAACAGGAGA AGATAGCTGT GTTTCGGGAT GCAAGCCGGT TCATTCTCAA TCTCACAAGC
     1681 GTGAAGCCGG AAGACAGTGG CATCTACTTC TGCATGATCG TCGGGAGCCC CGAGCTGACC
     1741 TTCGGGAAGG GAACTCAGCT GAGTGTGGTT GATTTCCTTC CCACCACTGC CCAGCCCACC
     1801 AAGAAGTCCA CCCTCAAGAA GAGAGTGTGC CGGTTACCCA GGCCAGAGAC CCAGAAGGGC
     1861 CCACTTTGTA GCCCCATCAC CCTTGGCCTG CTGGTGGCTG GCGTCCTGGT TCTGCTGGTT
     1921 TCCCTGGGAG TGGCCATCCA CCTGTGCTGC CGGCGGAGGA GAGCCCGGCT TCGTTTCATG
     1981 AAACAATTTT ACAAAGGCAG CGGCGCGACC AACTTTAGCC TGCTGAAACA GGCGGGCGAT
     2041 GTTGAAGAAA ACCCGGGCCC GGGTAAGGGG TGCTTATGTG ACTTAAGGTA GACATAAGGT
     2101 AGTGTGCCAG TTTAGTGCAT GTACGCTgat tgaaatcctg gttctgccac aaccatgtga
     2161 ccttgggtga gttactaaac ctctctgcac cttggtttca gcctctgtga aatggggatg
     2221 atgttaactg ccatagtgac tacctcgtat taagttgagg actgatatac gtaaggcact
     2281 gaaaatggtg cctggcacag agtaagccct agttaagtgt tcgctgttat tTTGTGAAGG
     2341 GTGATGAATA CGCCTCTAAG GAGTGGAGAC GCGTGCGGCC GCAGGAACCC CTAGTGATGG
     2401 AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG
     2461 CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCTGCCTGC
     2521 AGGACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
     2581 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
     2641 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
     2701 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
     2761 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
     2821 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
     2881 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
     2941 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
     3001 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
     3061 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
     3121 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
     3181 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
     3241 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
     3301 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
     3361 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
     3421 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
     3481 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
     3541 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
     3601 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
     3661 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
     3721 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
     3781 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
     3841 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
     3901 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
     3961 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
     4021 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCT
     4081 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     4141 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     4201 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     4261 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
     4321 AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG
     4381 CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC
     4441 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC
     4501 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA
     4561 GAGCAGATTG TACTGAGAGT GCACCATAAA ATTGTAAACG TTAATATTTT GTTAAAATTC
     4621 GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC
     4681 CCTTATAAAT CAAAAGAATA GCCCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG
     4741 AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC
     4801 GATGGCCCAC TACGTGAACC ATCACCCAAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA
     4861 GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG
     4921 AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT
     4981 GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC
     5041 GCGTACTATG GTTGCTTTGA CGTATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA
     5101 AATACCGCAT CAGGCGCC
//
LOCUS       TRAC-1G4-LY_SEED        4718 bp ds-DNA     circular     29-JUN-2024
DEFINITION  .
KEYWORDS    "Made by:Chris" "Vector:AAV6" "Locus:hTRAC" "Architecture:HIT"
            "Antigen target:CD19" "Co-Stim(s)" "Tag" "Binder linker"
            "Fluorescent protein" "Bacterial resistance:Kan" "Status:Virus,
            Maxi-Prep" "Notes:HIT with knocked out binding epitope for TCR
            antibody (Clone\: BW 242/412)"
FEATURES             Location/Qualifiers
     misc_feature    complement(1..141)
                     /label="Right ITR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    158..775
                     /label="Intron LHA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    776..835
                     /label="Polypyrimidine/branch point"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     https://www.... 776..839
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    837..839
                     /label="splice acceptor"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    840..841
                     /label="2 additional bases to place in frame"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(840..841)
                     /label="18-54: pIG-641 Linearize R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    842..907
                     /label="P2A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             908..1249
                     /label="TRBV6-5"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     misc_feature    989..991
                     /label="K9R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    992..994
                     /label="F10Y"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1043..1057
                     /label="CDR1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1064..1066
                     /label=41
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1109..1126
                     /label="CDR2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1235..1276
                     /label="CDR3"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1235..1276
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="CASSYVGNTGELFF"
     CDS             1256..1303
                     /label="TRBJ2-2"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     CDS             1307..1840
                     /label="TRBC2"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     CDS             1307..1840
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="DLKNVFPPKVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVSTDPQPLKEQPALNDSRYCLSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQKRAKPVTQIVSAEAWGRADCGFTSESYQQGVLSATILYEILLGKATLYAVLVSALVLMAMVKRKDSRG"
     misc_feature    1637..1639
                     /label="D112K"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1841..1906
                     /label="P2A*"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1841..1906
                     /label="Translation 1841-1906"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             1907..2242
                     /label="TRAV21"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     CDS             1907..2305
                     /label="Translation 1907-2305"
                     /translation="METLLGLLILWLQLQWVSSKQEVTQIPAALSVPEGENLVLNCSFTDSAIYNLQWFRQDPGKGLTSLLLIQSSQREQTSGRLNASLDKSSGRSTLYIAASQPGDSATYLCAVRPLYGGSYIPTFGRGTSLIVHP"
     CDS             2231..2275
                     /label="CDR3"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2231..2275
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="CAVRPLYGGSYIPTF"
     CDS             2252..2305
                     /label="TRAJ6"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     misc_feature    2306..2306
                     /label="Spacer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2307..2316
                     /label="SPLICE donor (from Exon 5 normally)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2317..2924
                     /label="RHA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     gene            2317..2924
                     /label="TRAC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     exon            2659..2924
                     /label="TRAC-202 Exon 6"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2661..2924
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="IQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFP"
     misc_feature    complement(2933..3065)
                     /label="Left ITR"
                     /ApEinfo_revcolor="#2046c0"
                     /ApEinfo_fwdcolor="#2046c0"
     misc_feature    3066..3097
                     /label="3' pIDTSmart homol"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3066..3638
                     /label="pIDTSmart PCR (A)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3124..3129
                     /label="Kan -35"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3148..3153
                     /label="Kan -10"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3633..3638
                     /label="removed BsmBI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3635..3635
                     /label="T to A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4070..4657
                     /label="origin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4694..4718
                     /label="5' pIDTSmart homol"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4698..4714
                     /label="m13 fwd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4711..4718)
                     /label="1.rev"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCTCT AGCTCGAggc aggaggtcgg aAAGAATAAA
      181 CAATGAGAGT CACATTAAAA ACACAAAATC CTACGGAAAT ACTGAAGAAT GAGTCTCAGC
      241 ACTAAGGAAA AGCCTCCAGC AGCTCCTGCT TTCTGAGGGT GAAGGATAGA CGCTGTGGCT
      301 CTGCATGACT CACTAGCACT CTATCACGGC CATATTCTGG CAGGGTCAGT GGCTCCAAct
      361 aacatttgtt tggtacttta cagtttatta aatagatgtt tatatggaga agctctcatt
      421 tctttctcag aagagcctgg ctaggaaggt ggatgaggca ccatattcat tttgcaggtg
      481 aaattcctga gatgtaagga gctgctgtga cttgctcaag gccttatatc gagtaaacgg
      541 tagtgctggg gcttagacgc aggtgttctg atttatagtt caAAACCTCT ATCAATGAGA
      601 GAGCAATCTC CTGGTAATGT GATAGATTTC CCAACTTAAT GCCAACATAC CATAAACCTC
      661 CCATTCTGCT AATGCCCAGC CTAAGTTGGG GAGACCACTC CAGATTCCAA GATGTACAGT
      721 TTGCTTTGCT GGGCCTTTTT CCCATGCCTG CCTTTACTCT GCCAGAGTTA TATTGctctt
      781 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagA
      841 TGGATCTGGA GCAACAAACT TCTCACTACT CAAACAAGCA GGTGACGTGG AGGAGAATCC
      901 CGGccccATG AGCATCGGCC tgctgtgctg cgccgccctg agcctgctgt gggccggccc
      961 cgtgaacgcc ggcgtgaccc agacccccaa gttccaggtg ctgaagaccg gccagagcat
     1021 gaccctgcag tgcgcccagg acatgaacca cgagtacatg agctggtaca gacaggaccc
     1081 cggcatgggc ctgagactga tccactacag cgtgggcgcc ggcatcaccg accagggcga
     1141 ggtgcccaac ggctacaacg tgagcagaag caccaccgag gacttccccc tgagactgct
     1201 gagcgccgcc cccagccaga ccagcgtgta cttctgcgcc agcagctacg tgggcaacac
     1261 cggcgagctg ttcttcggcg agggcagcag actgaccgtg ctggaggacc tgaagaacgt
     1321 gttccccccc aaggtggccg tgttcgagcc cagcgagGCT gagatcagcc acacccagaa
     1381 ggccaccctg gtgtgcctgg ccaccggctt ctaccccgac cacgtggagc tgagctggtg
     1441 ggtgaacggc aaggaggtgc acagcggcgt gagcaccgac ccccagcccc tgaaggagca
     1501 gcccgccctg aacgacagca gatactgcct gagcagcaga ctgagagtga gcgccacctt
     1561 ctggcagaac cccagaaacc acttcagatg ccaggtgcag ttctacggcc tgagcgagaa
     1621 cgacgagtgg acccagAAAa gagccaagcc cgtgacccag atcgtgagcg ccgaggcctg
     1681 gggcagagcc gactgcggct tcaccagcga gagctaccag cagggcgtgc tgagcgccac
     1741 catcctgtac gagatcctgc tgggcaaggc caccctgtac gccgtgctgg tgagcgccct
     1801 ggtgctgatg gccatggtga agagaaagga cagcaGAGGC GGCAGTGGAG CTACTAACTT
     1861 CAGCCTGCTG AAGCAGGCTG GAGACGTGGA GGAGAACCCT GGacCCATGG AGACCCTGCT
     1921 Gggcctgctg atcctgtggc tgcagctgca gtgggtgagc agcaagcagg aggtgaccca
     1981 gatccccgcc gccctgagcg tgcccgaggg cgagaacctg gtgctgaact gcagcttcac
     2041 cgacagcgcc atctacaacc tgcagtggtt cagacaggac cccggcaagg gcctgaccag
     2101 cctgctgctg atccagagca gccagagaga gcagaccagc ggcagactga acgccagcct
     2161 ggacaagagc agcggcagaa gcaccctgta catcgccgcc agccagcccg gcgacagcgc
     2221 cacctacctg tgcgccgtga gacccctgta cggcggcagc tacatcccca ccttcggcag
     2281 aggcaccagC CTGATCGTGC ACCCCTGTAA GTATGACTGG GGTTTTGAAG AAGATCCTAT
     2341 TAAATAAAAG AATAAGCAGT ATTATTAAGT AGCCCTGCAT TTCAGGTTTC CTTGAGTGGC
     2401 AGGCCAGGCC TGGCCGTGAA CGTTCACTGA AATCATGGCC TCTTGGCCAA GATTGATAGC
     2461 TTGTGCCTGT CCCTGAGTCC CAGTCCATCA CGAGCAGCTG GTTTCTAAGA TGCTATTTCC
     2521 CGTATAAAGC ATGAGACCGT GACTTGCCAG CCCCACAGAG CCCCGCCCTT GTCCATCACT
     2581 GGCATCTGGA CTCCAGCCTG GGTTGGGGCA AAGAGGGAAA TGAGATCATG TCCTAACCCT
     2641 GATCCTCTTG TCCCACAGAT ATCCAGAACC CTGACCCTGC CGTGTACCAG CTGAGAGACT
     2701 CTAAATCCAG TGACAAGTCT GTCTGCCTAT TCACCGATTT TGATTCTCAA ACAAATGTGT
     2761 CACAAAGTAA GGATTCTGAT GTGTATATCA CAGACAAAAC TGTGCTAGAC ATGAGGTCTA
     2821 TGGACTTCAA GAGCAACAGT GCTGTGGCCT GGAGCAACAA ATCTGACTTT GCATGTGCAA
     2881 ACGCCTTCAA CAACAGCATT ATTCCAGAAG ACACCTTCTT CCCCGCGGCC GCAGGAACCC
     2941 CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA
     3001 CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC
     3061 AGCTGCCtgc aggATAGCAG AAAGTCAAAA GCCTCCGCGT AAGAGGTTCC AACTTTCACC
     3121 ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT ATCGAGATTT TCAGGAGCTA
     3181 AGGAAGCTAA AATGAGCCAT ATTCAACGGG AAACGTCTTG CTTGAAGCCG CGATTAAATT
     3241 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
     3301 GTGCGACAAT CTATCGATTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
     3361 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGGCTAAAC TGGCTGACGG
     3421 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC
     3481 TCACCACTGC GATCCCAGGG AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
     3541 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
     3601 GTAATTGTCC TTTTAACGGC GATCGCGTAT TTCGaCTCGC TCAGGCGCAA TCACGAATGA
     3661 ATAACGGTTT GGTTGGTGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
     3721 AAGTCTGGAA AGAAATGCAT AAACTCTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
     3781 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
     3841 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG
     3901 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG
     3961 ATATGAATAA ATTGCAGTTT CACTTGATGC TCGATGAGTT TTTCTAATGA GGACCTAAAT
     4021 GTAATCACCT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT GCTGGCGTTT TTCCATAGGC
     4081 TCCGCCCCCC TGACGAGCAT CACAAAAATC GATGCTCAAG TCAGAGGTGG CGAAACCCGA
     4141 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
     4201 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
     4261 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
     4321 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     4381 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
     4441 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     4501 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TCGGAAAAAG
     4561 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     4621 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TTTTCTACCG
     4681 AAGAAAGGCC CACCCGTGTA AAACGACGGC CAGTTTAT
//
LOCUS       TRAC-CAR_SEED           5978 bp ds-DNA     circular     29-JUN-2024
DEFINITION  .
KEYWORDS    "Made by:Chris" "Vector:AAV6" "Locus:hTRAC" "Architecture:HIT"
            "Antigen target:CD19" "Co-Stim(s)" "Tag" "Binder linker"
            "Fluorescent protein" "Bacterial resistance:Kan" "Status:Virus,
            Maxi-Prep" "Notes:HIT with knocked out binding epitope for TCR
            antibody (Clone\: BW 242/412)"
FEATURES             Location/Qualifiers
     misc_feature    complement(1..141)
                     /label="Right ITR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    158..775
                     /label="Intron LHA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    776..835
                     /label="Polypyrimidine/branch point"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     https://www.... 776..839
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    837..839
                     /label="splice acceptor"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    840..841
                     /label="2 additional bases to place in frame"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(840..841)
                     /label="18-54: pIG-641 Linearize R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    842..907
                     /label="P2A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    908..961
                     /label="SP"
                     /ApEinfo_revcolor="#330abf"
                     /ApEinfo_fwdcolor="#330abf"
     CDS             908..961
                     /label="Translation 908-961"
                     /translation="MALPVTALLLPLALLLHA"
     misc_feature    962..1321
                     /label="CD19 VH"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    962..2362
                     /label="1928z"
                     /ApEinfo_revcolor="#182ac0"
                     /ApEinfo_fwdcolor="#182ac0"
     CDS             962..3565
                     /label="Translation 962-3565"
                     /translation="EVKLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTVTVSSGGGGSGGGGSGGGGSDIELTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEIKRAAAIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRSRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPRGSGATNFSLLKQAGDVEENPGPMLLLVTSLLLCELPHPAFLLIPRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMGSGATNFSLLKQAGDVEENPGP"
     misc_feature    1373..1690
                     /label="CD19 VL"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2075..2140
                     /label="ITAM 1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2189..2257
                     /label="ITAM 2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2282..2347
                     /label="ITAM 3"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2363..2428
                     /label="P2A*"
                     /ApEinfo_revcolor="#c06517"
                     /ApEinfo_fwdcolor="#c06517"
     misc_feature    2429..3499
                     /label="EGFRt"
                     /ApEinfo_revcolor="#0c7cc0"
                     /ApEinfo_fwdcolor="#0c7cc0"
     misc_feature    3500..3565
                     /label="P2A**"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3566..3566
                     /label="Extra base added to keep in frame downstream"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3567..3576
                     /label="SPLICE donor (from Exon 5 normally)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     gene            3577..4184
                     /label="TRAC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3577..4184
                     /label="RHA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            3919..4184
                     /label="TRAC-202 Exon 6"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3921..4184
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="IQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFP"
     misc_feature    complement(4193..4325)
                     /label="Left ITR"
                     /ApEinfo_revcolor="#2046c0"
                     /ApEinfo_fwdcolor="#2046c0"
     misc_feature    4326..4357
                     /label="3' pIDTSmart homol"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4326..4898
                     /label="pIDTSmart PCR (A)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4384..4389
                     /label="Kan -35"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4408..4413
                     /label="Kan -10"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4893..4898
                     /label="removed BsmBI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4895..4895
                     /label="T to A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    5330..5917
                     /label="origin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    5954..5978
                     /label="5' pIDTSmart homol"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    5958..5974
                     /label="m13 fwd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(5971..5978)
                     /label="1.rev"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCTCT AGCTCGAggc aggaggtcgg aAAGAATAAA
      181 CAATGAGAGT CACATTAAAA ACACAAAATC CTACGGAAAT ACTGAAGAAT GAGTCTCAGC
      241 ACTAAGGAAA AGCCTCCAGC AGCTCCTGCT TTCTGAGGGT GAAGGATAGA CGCTGTGGCT
      301 CTGCATGACT CACTAGCACT CTATCACGGC CATATTCTGG CAGGGTCAGT GGCTCCAAct
      361 aacatttgtt tggtacttta cagtttatta aatagatgtt tatatggaga agctctcatt
      421 tctttctcag aagagcctgg ctaggaaggt ggatgaggca ccatattcat tttgcaggtg
      481 aaattcctga gatgtaagga gctgctgtga cttgctcaag gccttatatc gagtaaacgg
      541 tagtgctggg gcttagacgc aggtgttctg atttatagtt caAAACCTCT ATCAATGAGA
      601 GAGCAATCTC CTGGTAATGT GATAGATTTC CCAACTTAAT GCCAACATAC CATAAACCTC
      661 CCATTCTGCT AATGCCCAGC CTAAGTTGGG GAGACCACTC CAGATTCCAA GATGTACAGT
      721 TTGCTTTGCT GGGCCTTTTT CCCATGCCTG CCTTTACTCT GCCAGAGTTA TATTGctctt
      781 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagA
      841 TGGATCTGGA GCAACAAACT TCTCACTACT CAAACAAGCA GGTGACGTGG AGGAGAATCC
      901 CGGccccatg gctctcccag tgactgccct actgcttccc ctagcgcttc tcctgcatgc
      961 agaggtgaag ctgcagcagt ctggggctga gctggtgagg cctgggtcct cagtgaagat
     1021 ttcctgcaag gcttctggct atgcattcag tagctactgg atgaactggg tgaagcagag
     1081 gcctggacag ggtcttgagt ggattggaca gatttatcct ggagatggtg atactaacta
     1141 caatggaaag ttcaagggtc aagccacact gactgcagac aaatcctcca gcacagccta
     1201 catgcagctc agcggcctaa catctgagga ctctgcggtc tatttctgtg caagaaagac
     1261 cattagttcg gtagtagatt tctactttga ctactggggc caagggacca cggtcaccgt
     1321 ctcctcaggt ggaggtggat caggtggagg tggatctggt ggaggtggat ctgacattga
     1381 gctcacccag tctccaaaat tcatgtccac atcagtagga gacagggtca gcgtcacctg
     1441 caaggccagt cagaatgtgg gtactaatgt agcctggtat caacagaaac caggacaatc
     1501 tcctaaacca ctgatttact cggcaaccta ccggaacagt ggagtccctg atcgcttcac
     1561 aggcagtgga tctgggacag atttcactct caccatcact aacgtgcagt ctaaagactt
     1621 ggcagactat ttctgtcaac aatataacag gtatccgtac acgtccggag gggggaccaa
     1681 gctggagatc aaacgggcgg ccgcaattga agttatgtat cctcctcctt acctagacaa
     1741 tgagaagagc aatggaacca ttatccatgt gaaagggaaa cacctttgtc caagtcccct
     1801 atttcccgga ccttctaagc ccttttgggt gctggtggtg gttggtggag tcctggcttg
     1861 ctatagcttg ctagtaacag tggcctttat tattttctgg gtgaggagta agaggagcag
     1921 gctcctgcac agtgactaca tgaacatgac tccccgccgc cccgggccca cccgcaagca
     1981 ttaccagccc tatgccccac cacgcgactt cgcagcctat cgctccagag tgaagttcag
     2041 caggagcgca gacgcccccg cgtaccagca gggccagaac cagctctata acgagctcaa
     2101 tctaggacga agagaggagt acgatgtttt ggacaagaga cgtggccggg accctgagat
     2161 ggggggaaag ccgagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga
     2221 taagatggcg gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg
     2281 gcacgatggc ctttaccagg gtCTCagtac agccaccaag gacacctacg acgcccttca
     2341 catgcaggcc ctgccccctc gcGGAAGCGG AGCTACTAAC TTCAGCCTGC TGAAGCAGGC
     2401 TGGAGACGTG GAGGAGAACC CTGGACCCat gcttctcctg gtgacaagcc ttctgctctg
     2461 tgagttacca cacccagcat tcctcctgat cccacgcaaa gtgtgtaacg gaataggtat
     2521 tggtgaattt aaagactcac tctccataaa tgctacgaat attaaacact tcaaaaactg
     2581 cacctccatc agtggcgatc tccacatcct gccggtggca tttaggggtg actccttcac
     2641 acatactcct cctctggacc cacaggaact ggatattctg aaaaccgtaa aggaaatcac
     2701 agggtttttg ctgattcagg cttggcctga aaacaggacg gacctccatg cctttgagaa
     2761 cctagaaatc atacgcggca ggaccaagca acatggtcag ttttctcttg cagtcgtcag
     2821 cctgaacata acatccttgg gattacgctc cctcaaggag ataagtgatg gagatgtgat
     2881 aatttcagga aacaaaaatt tgtgctatgc aaatacaata aactggaaaa aactgtttgg
     2941 gacctccggt cagaaaacca aaattataag caacagaggt gaaaacagct gcaaggccac
     3001 aggccaggtc tgccatgcct tgtgctcccc cgagggctgc tggggcccgg agcccaggga
     3061 ctgcgtctct tgccggaatg tcagccgagg cagggaatgc gtggacaagt gcaaccttct
     3121 ggagggtgag ccaagggagt ttgtggagaa ctctgagtgc atacagtgcc acccagagtg
     3181 cctgcctcag gccatgaaca tcacctgcac aggacgggga ccagacaact gtatccagtg
     3241 tgcccactac attgacggcc cccactgcgt caagacctgc ccggcaggag tcatgggaga
     3301 aaacaacacc ctggtctgga agtacgcaga cgccggccat gtgtgccacc tgtgccatcc
     3361 aaactgcacc tacggatgca ctgggccagg tcttgaaggc tgtcccacga atgggcctaa
     3421 gatcccgtcc atcgccactg ggatggtggg ggccctcctc ttgctgctgg tggtggccct
     3481 ggggatcggc ctcttcatgG GCAGCGGCGC GACCAACTTT AGCCTGCTGA AACAGGCGGG
     3541 CGATGTTGAA GAAAACCCGG GCCCGAGTAA GTATGACTGG GGTTTTGAAG AAGATCCTAT
     3601 TAAATAAAAG AATAAGCAGT ATTATTAAGT AGCCCTGCAT TTCAGGTTTC CTTGAGTGGC
     3661 AGGCCAGGCC TGGCCGTGAA CGTTCACTGA AATCATGGCC TCTTGGCCAA GATTGATAGC
     3721 TTGTGCCTGT CCCTGAGTCC CAGTCCATCA CGAGCAGCTG GTTTCTAAGA TGCTATTTCC
     3781 CGTATAAAGC ATGAGACCGT GACTTGCCAG CCCCACAGAG CCCCGCCCTT GTCCATCACT
     3841 GGCATCTGGA CTCCAGCCTG GGTTGGGGCA AAGAGGGAAA TGAGATCATG TCCTAACCCT
     3901 GATCCTCTTG TCCCACAGAT ATCCAGAACC CTGACCCTGC CGTGTACCAG CTGAGAGACT
     3961 CTAAATCCAG TGACAAGTCT GTCTGCCTAT TCACCGATTT TGATTCTCAA ACAAATGTGT
     4021 CACAAAGTAA GGATTCTGAT GTGTATATCA CAGACAAAAC TGTGCTAGAC ATGAGGTCTA
     4081 TGGACTTCAA GAGCAACAGT GCTGTGGCCT GGAGCAACAA ATCTGACTTT GCATGTGCAA
     4141 ACGCCTTCAA CAACAGCATT ATTCCAGAAG ACACCTTCTT CCCCGCGGCC GCAGGAACCC
     4201 CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA
     4261 CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC
     4321 AGCTGCCtgc aggATAGCAG AAAGTCAAAA GCCTCCGCGT AAGAGGTTCC AACTTTCACC
     4381 ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT ATCGAGATTT TCAGGAGCTA
     4441 AGGAAGCTAA AATGAGCCAT ATTCAACGGG AAACGTCTTG CTTGAAGCCG CGATTAAATT
     4501 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
     4561 GTGCGACAAT CTATCGATTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
     4621 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGGCTAAAC TGGCTGACGG
     4681 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC
     4741 TCACCACTGC GATCCCAGGG AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
     4801 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
     4861 GTAATTGTCC TTTTAACGGC GATCGCGTAT TTCGaCTCGC TCAGGCGCAA TCACGAATGA
     4921 ATAACGGTTT GGTTGGTGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
     4981 AAGTCTGGAA AGAAATGCAT AAACTCTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
     5041 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
     5101 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG
     5161 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG
     5221 ATATGAATAA ATTGCAGTTT CACTTGATGC TCGATGAGTT TTTCTAATGA GGACCTAAAT
     5281 GTAATCACCT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT GCTGGCGTTT TTCCATAGGC
     5341 TCCGCCCCCC TGACGAGCAT CACAAAAATC GATGCTCAAG TCAGAGGTGG CGAAACCCGA
     5401 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
     5461 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
     5521 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
     5581 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     5641 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
     5701 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     5761 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TCGGAAAAAG
     5821 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     5881 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TTTTCTACCG
     5941 AAGAAAGGCC CACCCGTGTA AAACGACGGC CAGTTTAT
//
LOCUS       TRAC-HIT_SEED           4718 bp ds-DNA     circular     29-JUN-2024
DEFINITION  .
KEYWORDS    "Made by:Chris" "Vector:AAV6" "Locus:hTRAC" "Architecture:HIT"
            "Antigen target:CD19" "Co-Stim(s)" "Tag" "Binder linker"
            "Fluorescent protein" "Bacterial resistance:Kan" "Status:Virus,
            Maxi-Prep" "Notes:HIT with knocked out binding epitope for TCR
            antibody (Clone\: BW 242/412)"
FEATURES             Location/Qualifiers
     misc_feature    complement(1..141)
                     /label="Right ITR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    158..775
                     /label="Intron LHA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    776..835
                     /label="Polypyrimidine/branch point"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     https://www.... 776..839
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    837..839
                     /label="splice acceptor"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    840..841
                     /label="2 additional bases to place in frame"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(840..841)
                     /label="18-54: pIG-641 Linearize R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    842..907
                     /label="P2A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    908..961
                     /label="SP"
                     /ApEinfo_revcolor="#330abf"
                     /ApEinfo_fwdcolor="#330abf"
     CDS             908..961
                     /label="Translation 908-961"
                     /translation="MALPVTALLLPLALLLHA"
     misc_feature    908..2305
                     /label="SEED Cargo"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    962..1321
                     /label="CD19-VH"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             962..1321
                     /label="Translation 962-1321"
                     /translation="EVKLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGDTNYNGKFKGQATLTADKSSSTAYMQLSGLTSEDSAVYFCARKTISSVVDFYFDYWGQGTTVTV"
     misc_feature    1322..1861
                     /label="TRBC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             1322..1861
                     /label="Translation 1322-1861"
                     /translation="LEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVSTDPQPLKEQPALNDSRYCLSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQKRAKPVTQIVSAEAWGRADCGFTSESYQQGVLSATILYEILLGKATLYAVLVSALVLMAMVKRKDSRG"
     misc_feature    1658..1660
                     /label="D112K"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1862..1927
                     /label="P2A"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1928..1981
                     /label="SP"
                     /ApEinfo_revcolor="#3300bf"
                     /ApEinfo_fwdcolor="#3300bf"
     misc_feature    1982..2299
                     /label="CD19-VL"
                     /ApEinfo_revcolor="#2dc519"
                     /ApEinfo_fwdcolor="#2dc519"
     CDS             1982..2299
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="DIELTQSPKFMSTSVGDRVSVTCKASQNVGTNVAWYQQKPGQSPKPLIYSATYRNSGVPDRFTGSGSGTDFTLTITNVQSKDLADYFCQQYNRYPYTSGGGTKLEI"
     misc_feature    2300..2305
                     /label="Adjustable"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2300..2305
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="IP"
     misc_feature    2306..2306
                     /label="Extra base added to keep in frame downstream"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2307..2316
                     /label="SPLICE donor (from Exon 5 normally)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     gene            2317..2924
                     /label="TRAC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2317..2924
                     /label="RHA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            2659..2924
                     /label="TRAC-202 Exon 6"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2661..2924
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="IQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFP"
     misc_feature    complement(2933..3065)
                     /label="Left ITR"
                     /ApEinfo_revcolor="#2046c0"
                     /ApEinfo_fwdcolor="#2046c0"
     misc_feature    3066..3097
                     /label="3' pIDTSmart homol"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3066..3638
                     /label="pIDTSmart PCR (A)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3124..3129
                     /label="Kan -35"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3148..3153
                     /label="Kan -10"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3633..3638
                     /label="removed BsmBI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3635..3635
                     /label="T to A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4070..4657
                     /label="origin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4694..4718
                     /label="5' pIDTSmart homol"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4698..4714
                     /label="m13 fwd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4711..4718)
                     /label="1.rev"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCTCT AGCTCGAggc aggaggtcgg aAAGAATAAA
      181 CAATGAGAGT CACATTAAAA ACACAAAATC CTACGGAAAT ACTGAAGAAT GAGTCTCAGC
      241 ACTAAGGAAA AGCCTCCAGC AGCTCCTGCT TTCTGAGGGT GAAGGATAGA CGCTGTGGCT
      301 CTGCATGACT CACTAGCACT CTATCACGGC CATATTCTGG CAGGGTCAGT GGCTCCAAct
      361 aacatttgtt tggtacttta cagtttatta aatagatgtt tatatggaga agctctcatt
      421 tctttctcag aagagcctgg ctaggaaggt ggatgaggca ccatattcat tttgcaggtg
      481 aaattcctga gatgtaagga gctgctgtga cttgctcaag gccttatatc gagtaaacgg
      541 tagtgctggg gcttagacgc aggtgttctg atttatagtt caAAACCTCT ATCAATGAGA
      601 GAGCAATCTC CTGGTAATGT GATAGATTTC CCAACTTAAT GCCAACATAC CATAAACCTC
      661 CCATTCTGCT AATGCCCAGC CTAAGTTGGG GAGACCACTC CAGATTCCAA GATGTACAGT
      721 TTGCTTTGCT GGGCCTTTTT CCCATGCCTG CCTTTACTCT GCCAGAGTTA TATTGctctt
      781 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagA
      841 TGGATCTGGA GCAACAAACT TCTCACTACT CAAACAAGCA GGTGACGTGG AGGAGAATCC
      901 CGGccccatg gctctcccag tgactgccct actgcttccc ctagcgcttc tcctgcatgc
      961 agaggtgaag ctgcagcagt ctggggctga gctggtgagg cctgggtcct cagtgaagat
     1021 ttcctgcaag gcttctggct atgcattcag tagctactgg atgaactggg tgaagcagag
     1081 gcctggacag ggtcttgagt ggattggaca gatttatcct ggagatggtg atactaacta
     1141 caatggaaag ttcaagggtc aagccacact gactgcagac aaatcctcca gcacagccta
     1201 catgcagctc agcggcctaa catctgagga ctctgcggtc tatttctgtg caagaaagac
     1261 cattagttcg gtagtagatt tctactttga ctactggggc caagggacca cggtcaccgt
     1321 cCTGGAGGAT CTGAAAAACG TGTTCCCTCC TGAAGTGGCT GTCTTTGAAC CATCCGAGGC
     1381 CGAGATTTCC CATACCCAGA AAGCAACTCT GGTCTGTCTG GCCACTGGAT TCTACCCCGA
     1441 TCACGTGGAA CTGTCTTGGT GGGTGAACGG CAAGGAAGTC CATTCCGGAG TCTCTACCGA
     1501 CCCTCAGCCC CTCAAGGAGC AGCCTGCTCT CAACGATTCT CGGTACTGCC TGTCATCTCG
     1561 ACTGAGAGTG TCTGCCACCT TCTGGCAGAA CCCTAGAAAC CACTTTCGGT GTCAGGTCCA
     1621 GTTTTACGGC CTGAGCGAGA ACGATGAGTG GACACAGAAA AGAGCCAAAC CTGTGACACA
     1681 GATTGTGAGC GCCGAGGCTT GGGGACGAGC CGATTGTGGC TTCACATCCG AGTCTTACCA
     1741 GCAGGGAGTG CTGTCTGCTA CAATCCTCTA CGAAATTCTC CTGGGGAAGG CCACCCTGTA
     1801 CGCTGTCCTC GTGTCTGCTC TGGTGCTCAT GGCTATGGTC AAACGAAAGG ACTCTAGAGG
     1861 CGGCTCTGGC GCTACCAATT TTTCCCTCCT CAAACAGGCT GGAGATGTCG AAGAGAACCC
     1921 CGGacctATG GCACTGCCCG TGACCGCTCT GCTTCTCCCA CTAGCTCTGC TTCTCCACGC
     1981 Agacattgag ctcacccagt ctccaaaatt catgtccaca tcagtaggag acagggtcag
     2041 cgtcacctgc aaggccagtc agaatgtggg tactaatgta gcctggtatc aacagaaacc
     2101 aggacaatct cctaaaccac tgatttactc ggcaacctac cggaacagtg gagtccctga
     2161 tcgcttcaca ggcagtggat ctgggacaga tttcactctc accatcacta acgtgcagtc
     2221 taaagacttg gcagactatt tctgtcaaca atataacagg tatccgtaca cgtccggagg
     2281 ggggaccaag ctggagatca ttcccAGTAA GTATGACTGG GGTTTTGAAG AAGATCCTAT
     2341 TAAATAAAAG AATAAGCAGT ATTATTAAGT AGCCCTGCAT TTCAGGTTTC CTTGAGTGGC
     2401 AGGCCAGGCC TGGCCGTGAA CGTTCACTGA AATCATGGCC TCTTGGCCAA GATTGATAGC
     2461 TTGTGCCTGT CCCTGAGTCC CAGTCCATCA CGAGCAGCTG GTTTCTAAGA TGCTATTTCC
     2521 CGTATAAAGC ATGAGACCGT GACTTGCCAG CCCCACAGAG CCCCGCCCTT GTCCATCACT
     2581 GGCATCTGGA CTCCAGCCTG GGTTGGGGCA AAGAGGGAAA TGAGATCATG TCCTAACCCT
     2641 GATCCTCTTG TCCCACAGAT ATCCAGAACC CTGACCCTGC CGTGTACCAG CTGAGAGACT
     2701 CTAAATCCAG TGACAAGTCT GTCTGCCTAT TCACCGATTT TGATTCTCAA ACAAATGTGT
     2761 CACAAAGTAA GGATTCTGAT GTGTATATCA CAGACAAAAC TGTGCTAGAC ATGAGGTCTA
     2821 TGGACTTCAA GAGCAACAGT GCTGTGGCCT GGAGCAACAA ATCTGACTTT GCATGTGCAA
     2881 ACGCCTTCAA CAACAGCATT ATTCCAGAAG ACACCTTCTT CCCCGCGGCC GCAGGAACCC
     2941 CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA
     3001 CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC
     3061 AGCTGCCtgc aggATAGCAG AAAGTCAAAA GCCTCCGCGT AAGAGGTTCC AACTTTCACC
     3121 ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT ATCGAGATTT TCAGGAGCTA
     3181 AGGAAGCTAA AATGAGCCAT ATTCAACGGG AAACGTCTTG CTTGAAGCCG CGATTAAATT
     3241 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
     3301 GTGCGACAAT CTATCGATTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
     3361 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGGCTAAAC TGGCTGACGG
     3421 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC
     3481 TCACCACTGC GATCCCAGGG AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
     3541 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
     3601 GTAATTGTCC TTTTAACGGC GATCGCGTAT TTCGaCTCGC TCAGGCGCAA TCACGAATGA
     3661 ATAACGGTTT GGTTGGTGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
     3721 AAGTCTGGAA AGAAATGCAT AAACTCTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
     3781 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
     3841 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG
     3901 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG
     3961 ATATGAATAA ATTGCAGTTT CACTTGATGC TCGATGAGTT TTTCTAATGA GGACCTAAAT
     4021 GTAATCACCT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT GCTGGCGTTT TTCCATAGGC
     4081 TCCGCCCCCC TGACGAGCAT CACAAAAATC GATGCTCAAG TCAGAGGTGG CGAAACCCGA
     4141 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
     4201 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
     4261 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
     4321 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     4381 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
     4441 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     4501 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TCGGAAAAAG
     4561 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     4621 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TTTTCTACCG
     4681 AAGAAAGGCC CACCCGTGTA AAACGACGGC CAGTTTAT
//