LOCUS       Supplementary_Da       16652 bp    DNA     circular SYN 01-AUG-2025
DEFINITION  .
ACCESSION   VB210727-1077men
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16652
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    complement(586..1174)
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="/vntifkey=21"
                     /note="color: #0375bb; direction: LEFT"
     misc_feature    complement(1345..2205)
                     /label=Amp
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=Amp"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=21"
                     /note="color: #6d3ed8; direction: LEFT"
     primer_bind     2933..2948
                     /label=M13-F
                     /note="color: 黒; sequence: gtaaaacgacggccag; added: 
                     2021-09-28"
     misc_feature    3037..3271
                     /label=3'ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Recognized by PBase transpose; 
                     DNAflankd by piggyBac 5' ITR and 3' ITR can be transposed 
                     by PBase into TTAA genomic sites."
                     /note="/name=3'ITR"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=22382df36c4b4754b7821e5d7876f273"
                     /note="/vntifkey=21"
                     /note="color: #ce0084; direction: RIGHT"
     misc_feature    complement(3481..4002)
                     /label=rBG pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/full_name=Rabbit beta-globin polyadenylation
                     signal"
                     /note="/name=rBG pA"
                     /note="/official_designation=rBG pA"
                     /note="/uuid=8fa32026eca4441f81c85dc922b69b4a"
                     /note="/vntifkey=21"
                     /note="color: #944603; direction: LEFT"
     CDS             complement(4151..4867)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used green 
                     fluorescentprotein; ranked high in brightness, 
                     photostability and pH stability among all fluorescent 
                     proteins."
                     /note="/full_name=Enhanced green fluorescent protein(ns)"
                     /note="/name=EGFP"
                     /note="/official_designation=EGFP(ns)"
                     /note="/uuid=5443fd992fa248b69b274cf75346abad"
                     /note="/vntifkey=4"
                     /note="color: #f54c6b"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    complement(4868..4873)
                     /label=HinCII
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=HinCII"
                     /note="/official_designation=HinCII"
                     /note="/uuid=e9cd34c1414443f587002f20408de72b"
                     /note="/vntifkey=21"
                     /note="color: #05696d; direction: LEFT"
     misc_feature    complement(4880..4945)
                     /label=P2A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=P2A"
                     /note="/official_designation=P2A"
                     /note="/uuid=c3788dff5caa4b648ad130604ef4ab47"
                     /note="/vntifkey=21"
                     /note="color: #7a00b5; direction: LEFT"
     misc_feature    complement(4955..4975)
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=df605fcd90d54706a01cbc052d116c2e"
                     /note="/vntifkey=21"
                     /note="color: #e4b930; direction: LEFT"
     misc_feature    complement(4976..4981)
                     /locus_tag="NheI(1)"
                     /label=NheI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=NheI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=efd7b4e467c64c70ae8bfad8c16a27cb"
                     /note="/vntifkey=21"
                     /note="color: #da3bb5; direction: LEFT"
     CDS             complement(4982..6484)
                     /locus_tag="Cse1"
                     /label=Cse1 (Cas8)
                     /label=Cse1
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Cse1"
                     /note="/official_designation=Cse1"
                     /note="/uuid=352d3027d7d542ba81a93a56e88a177a"
                     /note="/vntifkey=4"
                     /note="color: #eda481; direction: LEFT"
     misc_feature    complement(6485..6490)
                     /locus_tag="BamHI(1)"
                     /label=BamHI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BamHI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=2e24d26a052f4a8f9539513b7d1f974d"
                     /note="/vntifkey=21"
                     /note="color: #46c6ef; direction: LEFT"
     misc_feature    complement(6491..6511)
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=ce29df82b9044828bd79770a3198e2c6"
                     /note="/vntifkey=21"
                     /note="color: #fd3434; direction: LEFT"
     misc_feature    complement(6512..6514)
                     /label=XmaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=XmaI"
                     /note="/official_designation=XmaI"
                     /note="/uuid=2244014b4dcc422ca4bf548d659cc127"
                     /note="/vntifkey=21"
                     /note="color: #d05c0a; direction: LEFT"
     CDS             complement(6515..6568)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna viruscapsidprotein"
                     /label=T2A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=T2A"
                     /note="/official_designation=T2A"
                     /note="/uuid=04a8e2ffdb204869adb10d270b9a21a6"
                     /note="/vntifkey=4"
                     /note="color: #c54b7c"
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    complement(6578..6598)
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=8974eedfd23f470a9b602646922c896c"
                     /note="/vntifkey=21"
                     /note="color: #ef6cdf; direction: LEFT"
     misc_feature    complement(6599..6604)
                     /locus_tag="XbaI(1)"
                     /label=XbaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=XbaI"
                     /note="/official_designation=XbaI"
                     /note="/uuid=fbb65844604642bf898f6d6bc0ba2215"
                     /note="/vntifkey=21"
                     /note="color: #5566f5; direction: LEFT"
     CDS             complement(6605..7309)
                     /locus_tag="Cas5"
                     /label=Cas5
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Cas5"
                     /note="/official_designation=Cas5"
                     /note="/uuid=d14d7721cbd549a5bb606bd201bc3ace"
                     /note="/vntifkey=4"
                     /note="color: #f58600; direction: LEFT"
     misc_feature    complement(7310..7315)
                     /locus_tag="MluI(1)"
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=MluI"
                     /note="/official_designation=MluI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
                     /note="/vntifkey=21"
                     /note="color: #0c5d90; direction: LEFT"
     misc_feature    complement(7316..7336)
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=fc17bba5fcec419193b498e8690762ec"
                     /note="/vntifkey=21"
                     /note="color: #800c0c; direction: LEFT"
     misc_feature    complement(7340..7405)
                     /locus_tag="P2A(1)"
                     /label=P2A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=P2A"
                     /note="/official_designation=P2A"
                     /note="/uuid=c3788dff5caa4b648ad130604ef4ab47"
                     /note="/vntifkey=21"
                     /note="color: #db6901; direction: LEFT"
     misc_feature    complement(7406..7426)
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=793c8d88001445ea81d105e62523f42c"
                     /note="/vntifkey=21"
                     /note="color: #1f36a9; direction: LEFT"
     misc_feature    complement(7427..7432)
                     /locus_tag="SpeI(1)"
                     /label=SpeI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SpeI"
                     /note="/official_designation=SpeI"
                     /note="/uuid=fc8372cb4c1249e8a1abfca3c3762c5b"
                     /note="/vntifkey=21"
                     /note="color: #d84e4e; direction: LEFT"
     misc_feature    complement(7433..8554)
                     /locus_tag="Cas7"
                     /label=Cas7
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Cas7"
                     /note="/official_designation=Cas7"
                     /note="/uuid=7a535445040b4b7db1ab1e94028cb06a"
                     /note="/vntifkey=21"
                     /note="color: #512bbd; direction: LEFT"
     misc_feature    complement(8555..8560)
                     /locus_tag="BglII(1)"
                     /label=BglII
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BglII"
                     /note="/official_designation=BglII"
                     /note="/uuid=00295cad732446d0a5047be780aa021b"
                     /note="/vntifkey=21"
                     /note="color: #946a00; direction: LEFT"
     misc_feature    complement(8561..8581)
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Directs nuclear localization of 
                     taggedprotein."
                     /note="/full_name=SV40 nuclear localization signal"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40-NLS"
                     /note="/uuid=e0047c09bd0447d1850c7a5ea0c19171"
                     /note="/vntifkey=21"
                     /note="color: #a949ca; direction: LEFT"
     misc_feature    complement(8597..8602)
                     /locus_tag="EcoRI(1)"
                     /label=EcoRI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=EcoRI"
                     /note="/official_designation=EcoRI"
                     /note="/uuid=18b38e5fe9db4734be3e7bd8a74a83da"
                     /note="/vntifkey=21"
                     /note="color: #0375bb; direction: LEFT"
     misc_feature    complement(8603..9781)
                     /label=EF1A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter."
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor1 alpha1 promoter"
                     /note="/name=EF1A"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
                     /note="/vntifkey=21"
                     /note="color: #6d3ed8; direction: LEFT"
     promoter        9860..10102
                     /locus_tag="U6 promoter"
                     /label=U6 promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=U6 promoter"
                     /note="/official_designation=U6 promoter"
                     /note="/uuid=f56d01370c1f408b970c174a20ef8f29"
                     /note="/vntifkey=30"
                     /note="color: #ce0084; direction: RIGHT"
     primer_bind     10024..10043
                     /label=U6_seq_F
                     /note="color: 黒; sequence: gactatcatatgcttaccgt; added: 
                     2021-09-28"
     misc_feature    10103..10131
                     /locus_tag="crRNA repeat"
                     /label=crRNA repeat
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=crRNA repeat"
                     /note="/official_designation=crRNA repeat"
                     /note="/uuid=2e35483f7faa4447889334071353ca56"
                     /note="/vntifkey=21"
                     /note="color: #944603; direction: RIGHT"
     misc_feature    10134..10139
                     /label=BbsI
                     /note="color: #a6acb3"
     misc_feature    10146..10151
                     /label=BbsI
                     /note="color: #a6acb3"
     misc_feature    10154..10182
                     /locus_tag="crRNA repeat(1)"
                     /label=crRNA repeat
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=crRNA repeat"
                     /note="/official_designation=crRNA repeat"
                     /note="/uuid=2e35483f7faa4447889334071353ca56"
                     /note="/vntifkey=21"
                     /note="color: #f54c6b; direction: RIGHT"
     primer_bind     complement(10208..10224)
                     /locus_tag="SK primer"
                     /label=SK primer
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SK primer"
                     /note="/official_designation=SK primer"
                     /note="/uuid=c64b8febe936443fb235795b0b18ba1a"
                     /note="/vntifkey=28"
                     /note="color: #05696d; direction: LEFT"
     promoter        complement(10257..10275)
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Drives high-level transcription 
                     ofdownstream gene in E. coli host cells that express T7 RNA
                     polymerase; can also be used for in vitro transcription."
                     /note="/full_name=T7 promoter"
                     /note="/name=T7 promoter"
                     /note="/official_designation=T7 promoter"
                     /note="/uuid=10f4dc6ae94949d8bcdfd6113b90938e"
                     /note="/vntifkey=30"
                     /note="color: #7a00b5; direction: LEFT"
     primer_bind     complement(10285..10301)
                     /locus_tag="M13 fwd"
                     /label=M13 fwd
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=M13 fwd"
                     /note="/official_designation=M13 fwd"
                     /note="/uuid=a870ee6c703b4426b89044276961f9f1"
                     /note="/vntifkey=28"
                     /note="color: #e4b930; direction: LEFT"
     primer_bind     complement(10286..10301)
                     /label=M13-F
                     /note="color: 黒; sequence: gtaaaacgacggccag; added: 
                     2021-09-28"
     misc_feature    10302..11480
                     /label=EF1A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Strong promoter."
                     /note="/name=EF1A"
                     /note="/official_designation=EF1A"
                     /note="/uuid=92f8a91ae7574711aa785bc7707cf8c1"
                     /note="/vntifkey=21"
                     /note="color: #da3bb5; direction: RIGHT"
     misc_feature    complement(11484..11489)
                     /locus_tag="EcoRI"
                     /label=EcoRI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=EcoRI"
                     /note="/official_designation=EcoRI"
                     /note="/uuid=18b38e5fe9db4734be3e7bd8a74a83da"
                     /note="/vntifkey=21"
                     /note="color: #eda481; direction: LEFT"
     misc_feature    11505..11525
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=baa49fc61e9f4e50adf8ebae99ae6177"
                     /note="/vntifkey=21"
                     /note="color: #46c6ef; direction: RIGHT"
     misc_feature    complement(11526..11531)
                     /locus_tag="BglII"
                     /label=BglII
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BglII"
                     /note="/official_designation=BglII"
                     /note="/uuid=00295cad732446d0a5047be780aa021b"
                     /note="/vntifkey=21"
                     /note="color: #fd3434; direction: LEFT"
     misc_feature    11532..12044
                     /locus_tag="Cse2"
                     /label=Cse2 (Cas11)
                     /label=Cse2
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Cse2"
                     /note="/official_designation=Cse2"
                     /note="/uuid=45b22c183b874e2eaff781037530f503"
                     /note="/vntifkey=21"
                     /note="color: #d05c0a; direction: RIGHT"
     misc_feature    complement(12045..12050)
                     /locus_tag="SpeI"
                     /label=SpeI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SpeI"
                     /note="/official_designation=SpeI"
                     /note="/uuid=fc8372cb4c1249e8a1abfca3c3762c5b"
                     /note="/vntifkey=21"
                     /note="color: #c54b7c; direction: LEFT"
     misc_feature    12051..12071
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=4aff023cf26244c8a52248d25b504d18"
                     /note="/vntifkey=21"
                     /note="color: #ef6cdf; direction: RIGHT"
     misc_feature    12072..12137
                     /locus_tag="P2A"
                     /label=P2A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=P2A"
                     /note="/official_designation=P2A"
                     /note="/uuid=841b4aae6b2143d8979543eb77ed9a9d"
                     /note="/vntifkey=21"
                     /note="color: #5566f5; direction: RIGHT"
     misc_feature    12141..12161
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=680f93e6812b40e6a0d07f5ac936c591"
                     /note="/vntifkey=21"
                     /note="color: #f58600; direction: RIGHT"
     misc_feature    complement(12162..12167)
                     /locus_tag="MluI"
                     /label=MluI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=MluI"
                     /note="/official_designation=MluI"
                     /note="/uuid=157d822145f14df2a23981abab96a158"
                     /note="/vntifkey=21"
                     /note="color: #0c5d90; direction: LEFT"
     misc_feature    12168..12797
                     /locus_tag="Cas6"
                     /label=Cas6
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Cas6"
                     /note="/official_designation=Cas6"
                     /note="/uuid=080757688c3b4a30b76a1f71a62814a1"
                     /note="/vntifkey=21"
                     /note="color: #800c0c; direction: RIGHT"
     misc_feature    complement(12798..12803)
                     /locus_tag="XbaI"
                     /label=XbaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=XbaI"
                     /note="/official_designation=XbaI"
                     /note="/uuid=fbb65844604642bf898f6d6bc0ba2215"
                     /note="/vntifkey=21"
                     /note="color: #db6901; direction: LEFT"
     misc_feature    12804..12824
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=d07f6fdeed044600971963ed09a2db94"
                     /note="/vntifkey=21"
                     /note="color: #1f36a9; direction: RIGHT"
     CDS             12834..12887
                     /codon_start=1
                     /product="2A peptide from Thosea asigna viruscapsidprotein"
                     /label=T2A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=T2A"
                     /note="/official_designation=T2A"
                     /note="/uuid=fdd34b687474446e8d2601cf49db9133"
                     /note="/vntifkey=4"
                     /note="color: #d84e4e"
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    complement(12888..12890)
                     /label=XmaI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=XmaI"
                     /note="/official_designation=XmaI"
                     /note="/uuid=76b6f682a9d5493e8ac930c424d86b5e"
                     /note="/vntifkey=21"
                     /note="color: #512bbd; direction: LEFT"
     misc_feature    12891..12911
                     /label=SV40_NLS
                     /label=SV40_NLS(opt)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS(opt)"
                     /note="/official_designation=SV40_NLS(opt)"
                     /note="/uuid=15240ffdee5643ab907e636b92548599"
                     /note="/vntifkey=21"
                     /note="color: #946a00; direction: RIGHT"
     misc_feature    complement(12912..12917)
                     /locus_tag="BamHI"
                     /label=BamHI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BamHI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=2e24d26a052f4a8f9539513b7d1f974d"
                     /note="/vntifkey=21"
                     /note="color: #a949ca; direction: LEFT"
     misc_feature    12918..15614
                     /locus_tag="Cas3"
                     /label=Cas3
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Cas3"
                     /note="/official_designation=Cas3"
                     /note="/uuid=d5abc207980b4c4ca92adc877144e465"
                     /note="/vntifkey=21"
                     /note="color: #0375bb; direction: RIGHT"
     misc_feature    complement(15615..15620)
                     /locus_tag="NheI"
                     /label=NheI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=NheI"
                     /note="/official_designation=BamHI"
                     /note="/uuid=efd7b4e467c64c70ae8bfad8c16a27cb"
                     /note="/vntifkey=21"
                     /note="color: #6d3ed8; direction: LEFT"
     misc_feature    15621..15641
                     /locus_tag="SV40_NLS(4)"
                     /label=SV40_NLS
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40_NLS"
                     /note="/official_designation=SV40_NLS"
                     /note="/uuid=15240ffdee5643ab907e636b92548599"
                     /note="/vntifkey=21"
                     /note="color: #ce0084; direction: RIGHT"
     misc_feature    complement(15645..15652)
                     /locus_tag="NotI"
                     /label=NotI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows linearization of vector."
                     /note="/name=NotI"
                     /note="/official_designation=NotI"
                     /note="/uuid=9f9f0cb60ddb47d7b6198deeaf89eaba"
                     /note="/vntifkey=21"
                     /note="color: #944603; direction: LEFT"
     misc_feature    15686..16226
                     /locus_tag="Rabbit hemoglobin beta poly A"
                     /label=Rabbit hemoglobin beta poly A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Rabbit hemoglobin beta poly A"
                     /note="/official_designation=Rabbit hemoglobin beta poly A"
                     /note="/uuid=eadfaadfce5e44e0bf86fc571be6453c"
                     /note="/vntifkey=21"
                     /note="color: #f54c6b; direction: RIGHT"
     misc_feature    complement(16340..16652)
                     /label=5'ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Recognized by PBase transpose; 
                     DNAflankd by piggyBac 5' ITR and 3' ITR can be transposed 
                     by PBase into TTAA genomic sites."
                     /note="/name=5'ITR"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=cf6f8504beb5412ca461e47810e15037"
                     /note="/vntifkey=21"
                     /note="color: #05696d; direction: LEFT"
ORIGIN
        1 aaaagatttg cgctttactc gacctaaact ttaaacacgt catagaatct tcgtttgaca
       61 aaaaccacat tgtggccaag ctgtgtgacg cgacgcgcgc taaagaatgg caaaccaagt
      121 cgcgcgaggt accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
      181 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      241 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      301 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      361 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      421 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      481 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      541 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      601 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      661 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      721 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      781 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      841 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      901 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      961 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1021 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1081 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1141 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1201 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1261 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1321 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1381 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1441 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1501 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1561 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1621 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     1681 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1741 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1801 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1861 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1921 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1981 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2041 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2101 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2161 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2221 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2281 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     2341 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2401 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2461 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2521 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2581 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     2641 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     2701 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     2761 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     2821 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     2881 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     2941 acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg tattcacgac
     3001 agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag ataatcatat
     3061 tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta tcggtctgta
     3121 tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt acacttacat
     3181 actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaaacaa
     3241 aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct tttacaaaaa
     3301 taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt aattagctta
     3361 acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac tttggcacat
     3421 atcaatatta tgctctcgtt aattaactcg agggatccaa gcttgggctg caggtcgagg
     3481 gatctccata agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat
     3541 ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa
     3601 caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca
     3661 gcagggggct gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc
     3721 agcatatggg catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga
     3781 tttgccctcc catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca
     3841 atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc
     3901 ccataatttt tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc
     3961 acaccagcca ccaccttctg ataggcagcc tgcacctgag gatcaactat gtataataaa
     4021 gttgtaactc gaggtatact taagcctagg atccagatct gtacattcga atcgatgcat
     4081 ggggtcgtgc gctcctttcg gtcgggcgct gcgggtcgtg gggcgggcga ttaccctgtt
     4141 atccctatta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     4201 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     4261 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     4321 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     4381 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     4441 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     4501 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     4561 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     4621 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     4681 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     4741 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     4801 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     4861 tcaccatgtc gacaagcttg gggcctggat tctcctccac atcgccggcc tgcttcagca
     4921 ggctgaagtt ggttgctccg cttccgccgc tgcccacctt gcgcttcttc ttggggctag
     4981 ctccattaga aggtcctccc tgtggcttca gctccctcag gtgcttatac agggtggccc
     5041 ttgccagggc cagtgtgctg atcagcttgg ggtggtgggc gtatggggcc acagactggt
     5101 taaacagcat ctcgcacagc tggtgcagct tgtccctcag atcggcgatc acttcgtcgg
     5161 cctgggagaa gttcacattg gccagcacat cggggatcag cagctcggac tggcggtaaa
     5221 agtgcctctc ggcggtctcg tgcacgctca cgcctgctcc cttgaagtcc ttgttcttaa
     5281 agccctcggc gaatgtatac agggcctttc tcagggcggt cttgtagccc aggcccactg
     5341 tcacgatctc gttgatcaca ttgccatact gctgccagcc ctgattaaac atcagcacat
     5401 cgtgtctccg ctccaggatg ctggcctgat tgttcctgta gccgcccatg atcagctcca
     5461 ggggagactg tggggcgatg ttcctgaact gattcaccac ggcggccact ctgttgccat
     5521 tctcgttctg gatgatcttg tccaccacca ctctgctgat ctgggtccaa gagggggcgg
     5581 atgtggtaaa ggccaggaac ttctcctcca cctcgccctt cttcactgtc accagacatg
     5641 gagagtgagg gtggggccac aggccgttca ctgtaaaggt gaacttctcc ttcaggaagc
     5701 cggtgtacct cagattgctt tcctggccac agcaagaaca cttgccgatg ccgatggggt
     5761 cgcacagctc gatgtgggct ggctgccaaa acaggcctct cacgaagccg atggagctgg
     5821 cagggatgct ctcgttagac ttgataggct tgatccaggt gggctggttc tctgtgtggc
     5881 tctcattggg aaactgcttc tgcagccgtg gcagggtcag cacattcagc agcactgtgc
     5941 tcctcagatc gatgcctctc acgaatgtgg tcactggggt tcctcccctc agtccagact
     6001 taaagcctcc tccgaatcca ggtgcctggt ttgcctgatt aaacagggcg atggcggtac
     6061 agcctccgca cagggcctct ccctgtcctg gctggttcac gaaggcgcaa tttgttgctc
     6121 cggacacgcc tgccagcagc ttctccatgg gggtcacgtc gttggccttc acgccctttg
     6181 tctgcatgaa ggggtgctcg gcgtgattca gatagaacat gtcgatccat ggggcgatca
     6241 gctgctgaaa ctcatcctcg gtcagagggt tcatgatgcg gtgcctgaac tccacatcgt
     6301 ccttggcagg ggcgatgatc tggccgatgc acaccagcag ggccagggcg gccagctcca
     6361 tatcgtctct gggcaggctc agtctccact gatcccggga gcagtacagg ctctgcagat
     6421 taataatctg gacttttcct ccgttccgtg ggcgcacagg aatccagttg tcaatcagca
     6481 ggttggatcc caccttcctc ttcttcttag gcccagggcc gggattctcc tccacgtcac
     6541 cgcatgttag cagacttcct ctgccctcgc cgctgcccac ctttctcttc ttcttaggtc
     6601 tagaggactc aaattcactg ccatcagcag tccgcttctg gctcacgtcc attcctccct
     6661 tgatcacata ccactccctg gatgcaaact gccttggcag tgtgatcatt ggctcatccc
     6721 tggcggtgaa cttcaggtgg tggcctgtca cgctctcctc agaatagatg tcgccgccca
     6781 caggctcgta gttcagcagg gccttctggg gatcggaggc ctgacaggtg cccaggaaca
     6841 gtgggtgtgt cagagggcag cttctccggc ccagataggg ggtgtaccgt ggcttcagca
     6901 cggccttctc cagctcgctg atgaccattg ttgcgtgtgg ggtcagccac agggccactg
     6961 taaaagaggc gtcgcacagg tactcgcgcc atgtctggat ggtctcgtgg gacttcaggc
     7021 ccctatagtc ctctctggcg cccagcacgg tgtggtaatc ccgcaggcct gtcacggaca
     7081 cgcgcctatc gtccaggatc agctcatcac agcgcacggc gaactgcacg gactcgctca
     7141 gggcctgcag gctagaggta tcgtccctct ggatgcccag gcaggctccc agcagtccca
     7201 gcagtccaga ccgggtagga aagcggcctg ttggcctggt tccctcgaat gtaggctgtc
     7261 cccatgcctg cattggtcct gccagtctca ggatcaggta ggagcgttca cgcgtcacct
     7321 tccgcttctt cttgggcatg gggcctggat tctcctccac atcgccggcc tgcttcagca
     7381 ggctgaagtt ggttgctccg cttcccacct tcctcttctt cttgggacta gtggactcaa
     7441 attcactgcc atcagcagtc cgctttgcct ctccattgtt ccgcacccag ctcttcagct
     7501 gctccagggt tggcatctgc ttcacctggg ctgtgatagg gtccacatca gacagggaaa
     7561 actgggcggc agctccgttc aggccatatc cattggccac gcggtcccag tactggttaa
     7621 aggcctggat ggaaggctgc aggaagccat ccttggcctt cacggccttc tcgaaggcat
     7681 tggccataga cagtggcatg tcggaaaagt tcaccatgac catatcggca ggattgaagg
     7741 cggcgtaggt gcgctgcttt gctcctggca cctcggttgc cagcatgtgc accacgtgtg
     7801 tggcgatctc cagggcctgc tccctggatg ctcctcccag gttctcctgc agctgtgcca
     7861 ggttgatatt ggcatatctg taaaacacgc cggagctgaa ctcctgggtt cccaggtggg
     7921 cgcttccctg ctcctgcaga tcgtccacgg ctgtgaacca gtcgatatca gagtccacct
     7981 ggtgtgtggt gatggcgtgt gcgatggaca ttgctccgtc caccttgccc agctcggtca
     8041 tcatgccgga tgtggccatc ctgccgctca gggcgatgtc cactccctgc tgcaggttca
     8101 ctctgatggc ggcgatatcc tccttcagca ccttcagcag cttcttatcg tccagattat
     8161 cggcctcggc cttggccacc tgctcacaga accatgcgat ctctcccacc acccatgggg
     8221 tcactgcgtc ggcgctgatc ttctcggcct catccacgga cttgccgctc agcagggcca
     8281 gtgtcttgtc gatgatcttc tgatcaaacc gctcgcccag cttctgtctc agcacgtccc
     8341 tcagctgtgc caggtggatg gtcctcagag aggactcgcc gatattctgg gcatagtagc
     8401 cagactttct catggcccgc ttcagggact ggctagagat cctcactctc cgcttgccgc
     8461 caaagatggc gtccttctgc atgttcatat cgtctctatt caggcagctt ggagagtggg
     8521 agatcagcac gtggatgttg atgaaattgg attcagatct cacctttctc ttcttcttgg
     8581 gggccatggt ggcggcgaat tctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca
     8641 ctgtctgagg cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga
     8701 attacatcaa gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact
     8761 ccatcgcata aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact
     8821 aatcgaggtg cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg
     8881 acggaaaggc ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg
     8941 tcctccattt tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa
     9001 ctggtgccga ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgagac
     9061 caggcaccag agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg
     9121 ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg
     9181 cggccccaaa aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt
     9241 gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga
     9301 aagcagcgag acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag
     9361 gccaggcctc aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct
     9421 cccacccact tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag
     9481 taattcaagg cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca
     9541 cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta
     9601 ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat
     9661 cactttccca gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc
     9721 cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc
     9781 cccactttgt acaagaaagc tgggtgcttt gcaaagatgg ataaagtttt aaacagagag
     9841 gaatctttgc aggcgcgcct tcttcccatg attccttcat atttgcatat acgatacaag
     9901 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     9961 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
    10021 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
    10081 ttgtggaaag gacgaaacac cggtgttccc cgcgccagcg gggataaacc ggggtcttcg
    10141 ctagcgaaga cctgtgttcc ccgcgccagc ggggataaac cgttttttga attcctgcag
    10201 cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca attcgcccta
    10261 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta cggctccggt gcccgtcagt
    10321 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
    10381 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
    10441 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
    10501 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
    10561 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
    10621 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
    10681 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
    10741 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
    10801 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
    10861 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
    10921 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
    10981 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggtctcgcg ccgccgtgta
    11041 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
    11101 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
    11161 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
    11221 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
    11281 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
    11341 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
    11401 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
    11461 ttccatttca ggtgtcgtga cgggaattcg ccgccaccat ggcccccaag aagaagagaa
    11521 aagtgagatc tgaagccgat gagatcgacg caatggcact gtacagggca tggcagcagc
    11581 tggacaacgg atcttgcgca cagatcaggc gcgtgagcga gcctgatgag ctgagggaca
    11641 tcccagcctt ctatcggctg gtgcagccct ttggctggga gaatcctaga caccagcagg
    11701 ccctgctgag gatggtgttt tgtctgagcg ccggcaagaa cgtgatccgg caccaggaca
    11761 agaagagcga gcagaccaca ggaatctccc tgggacgcgc cctggccaat tccggccgga
    11821 tcaacgagcg gagaatcttc cagctgatca gggccgatcg cacagccgac atggtgcagc
    11881 tgaggcgcct gctgacccac gcagagcctg tgctggattg gccactgatg gcccgcatgc
    11941 tgacatggtg gggcaagcgg gagagacagc agctgctgga ggacttcgtg ctgaccacaa
    12001 ataagaacgc caagcggact gctgatggca gtgaatttga gtccactagt ccaaagaaga
    12061 agcggaaggt gggaagcgga gcaaccaact tcagcctgct gaagcaggcc ggcgatgtgg
    12121 aggagaatcc aggccccatg cctaagaaga agcggaaggt gacgcgtgaa tacctgagca
    12181 aagtgatcat cgcaagggca tggtccaggg acctgtatca gctgcaccag ggcctgtggc
    12241 acctgttccc taatagacca gatgccgcca gggacttcct gtttcacgtg gagaagagga
    12301 acacacccga gggctgtcac gtgctgctgc agtccgccca gatgcccgtg agcaccgcag
    12361 tggccacagt gatcaagacc aagcaggtgg agttccagct gcaagtgggc gtgccactgt
    12421 actttaggct gcgcgccaat cccatcaaga ccatcctgga taaccagaag cgcctggact
    12481 ctaagggcaa tatcaagcgg tgcagagtgc ctctgatcaa ggaggccgag cagatcgcct
    12541 ggctgcagag aaagctgggc aacgccgcca gggtggagga tgtgcaccct atcagcgagc
    12601 ggccacagta tttcagcggc gacggcaagt ccggcaagat ccagaccgtg tgctttgagg
    12661 gcgtgctgac catcaacgat gccccagccc tgatcgacct ggtgcagcag ggaatcggac
    12721 ctgctaagtc aatgggatgt gggctgctgt cactggcacc tctgaagcgg actgctgatg
    12781 gcagtgaatt tgagtcctct agaccaaaga agaagaggaa ggtgggcagc ggcgagggca
    12841 gaggaagtct gctaacatgc ggtgacgtgg aggagaatcc cggccctggg ccaaagaaga
    12901 agagaaaggt gggatccgag gagcccttca agtacatctg ccactattgg ggcaagagct
    12961 ccaagagcct gacaaagggc aacgatatcc acctgctgat ctaccactgc ctggacgtgg
    13021 cagcagtggc agattgttgg tgggaccagt ccgtggtgct gcagaatacc ttttgtcgga
    13081 acgagatgct gtctaagcag agagtgaagg cctggctgct gttctttatc gccctgcacg
    13141 atatcggcaa gttcgacatc cggtttcagt ataagtccgc cgagtcttgg ctgaagctga
    13201 atccagccac accctctctg aacggcccta gcacccagat gtgcagaaag ttcaatcacg
    13261 gcgccgccgg cctgtactgg tttaaccagg actctctgag cgagcagagc ctgggcgatt
    13321 tcttttcctt ctttgacgca gcacctcacc cctacgagtc ctggttccct tgggtggagg
    13381 cagtgaccgg acaccacggc tttatcctgc attctcagga ccaggataag agccggtggg
    13441 agatgccagc cagcctggcc tcctacgccg cacaggataa gcaggccaga gaggagtgga
    13501 tcagcgtgct ggaggccctg ttcctgaccc cagcaggact gtccatcaat gatatcccac
    13561 ctgactgctc tagcctgctg gccggctttt gttccctggc cgactggctg ggctcttgga
    13621 ccacaaccaa tacattcctg tttaacgagg atgcaccaag cgacatcaac gccctgagga
    13681 cctacttcca ggatcggcag caggacgcct ccagagtgct ggagctgtcc ggcctggtgt
    13741 ctaataagag gtgctacgag ggagtgcacg ccctgctgga taacggctat cagccaaggc
    13801 agctgcaggt gctggtggac gccctgcctg tggcaccagg actgacagtg atcgaggcac
    13861 ctaccggcag cggcaagaca gagaccgccc tggcctatgc ctggaagctg atcgatcagc
    13921 agatcgccga ctccgtgatc ttcgccctgc caacacaggc caccgccaat gcaatgctga
    13981 cccggatgga ggcctccgcc tctcacctgt tttcctctcc caacctgatc ctggcccacg
    14041 gcaacagcag gttcaatcac ctgtttcagt ctatcaagag ccgcgccatc acagagcagg
    14101 gacaggagga ggcctgggtg cagtgctgtc agtggctgag ccagtccaat aagaaggtgt
    14161 tcctgggcca gatcggcgtg tgcaccatcg atcaggtgct gatctctgtg ctgcctgtga
    14221 agcacaggtt tatcagggga ctgggaatcg gcagaagcgt gctgatcgtg gatgaggtgc
    14281 acgcctacga cacatatatg aacggcctgc tggaggccgt gctgaaggca caggcagacg
    14341 tgggcggctc cgtgatcctg ctgtctgcca ccctgccaat gaagcagaag cagaagctgc
    14401 tggatacata cggcctgcac accgaccccg tggagaacaa ttccgcctat cctctgatca
    14461 attggagggg agtgaacgga gcacagcggt tcgacctgct ggcccaccct gagcagctgc
    14521 caccaaggtt ttctatccag cccgagccta tctgcctggc cgatatgctg ccagacctga
    14581 ccatgctgga gaggatgatc gctgccgcca atgcaggagc acaggtgtgc ctgatctgta
    14641 acctggtgga tgtggcccag gtgtgctacc agaggctgaa ggagctgaac aatacacagg
    14701 tggacatcga tctgttccac gcccggttta ccctgaatga ccggagagag aaggagaaca
    14761 gagtgatctc taacttcggc aagaatggca agagaaacgt gggcagaatc ctggtggcca
    14821 cacaggtggt ggagcagagc ctggacgtgg atttcgactg gctgatcacc cagcactgcc
    14881 ccgccgatct gctgtttcag cggctgggca gactgcacag gcaccaccgc aagtatcggc
    14941 ctgccggctt tgagatccca gtggccacaa tcctgctgcc tgacggagag ggatacggca
    15001 ggcacgagca catctatagc aatgtgcgcg tgatgtggcg gacccagcag cacatcgagg
    15061 agctgaacgg cgcctctctg ttctttccag atgcctaccg ccagtggctg gacagcatct
    15121 atgacgatgc cgagatggat gagcccgagt gggtgggcaa tggcatggac aagttcgagt
    15181 ccgccgagtg tgagaagcgg ttcaaggcca ggaaggtgct gcagtgggcc gaggagtact
    15241 ccctgcagga taacgacgag acaatcctgg ccgtgaccag agatggcgag atgtctctgc
    15301 ccctgctgcc ttatgtgcag acaagctccg gcaagcagct gctggatggc caggtgtacg
    15361 aggacctgag ccacgagcag cagtatgagg ccctggccct gaacagggtg aatgtgccct
    15421 tcacctggaa gcggagcttc agcgaagtgg tggatgagga cggcctgctg tggctggagg
    15481 gcaagcagaa tctggatggc tgggtgtggc agggcaactc catcgtgatc acatacaccg
    15541 gcgacgaggg catgaccaga gtgatcccag ccaaccccaa gaagagaacc gccgacggca
    15601 gcgagttcga gtccgctagc ccaaagaaga agagaaaggt gtaagcggcc gcagcctgct
    15661 tttttgtaca aacttgctcg agttaaattc actcctcagg tgcaggctgc ctatcagaag
    15721 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
    15781 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
    15841 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
    15901 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
    15961 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
    16021 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
    16081 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
    16141 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
    16201 ctcttatgga gatccctcga cttaattaac caacaagctc gtcatcgctt tgcagaagag
    16261 cagagaggat atgctcatcg tctaaagaac tacccatttt attatatatt agtcaccaac
    16321 ttttctatac aaagttgatg atatctataa caagaaaata tatatataat aagttatcac
    16381 gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta aaagtcacgt
    16441 aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa tcagtgacac
    16501 ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga tgtcctaaat
    16561 gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga atgcatgcgt
    16621 caattttacg cagactatct ttctagggtt aa
//
