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["Original Research Article"]
["Molecular dissection of transcriptional reprogramming of steviol glycosides synthesis in leaf tissue during developmental phase transitions in Stevia rebaudiana Bert"]
["Gopal Singh1,2, Gagandeep Singh1, Pradeep Singh1, Rajni Parmar1,2, Navgeet Paul1, Radhika Vashist1, Mohit Kumar Swarnkar1, Ashok Kumar3, Sanatsujat Singh3, Anil Kumar Singh1†, Sanjay Kumar1, Ram Kumar Sharma1,2* "]
["1Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 2Academy of Scientific and Innovative Research, New Delhi, India, 3Agrotechnology of Medicinal, Aromatic and Commercially Important Plants, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, †Present address: ICAR-Indian Institute of Agricultural Biotechnology, PDU Campus, IINRG, Namkum, Ranchi, Jharkhand, India"]
[]
["Table S9 List of primer sequences of selected transcripts used for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis"]
[]
["Sr. No","Gene ","Full name","Transcript ID","Expression primer sequences","Length","Tm (°C)"]
[1,"GAPDH","Glyceraldehyde-3-phosphate dehydrogenase","stevia5_contig_58","Fwd 5'-CCAGCCTTAGCATCAAAGAT-3'\nRev 5'-GCCATTAAGGAGGAATCAGA-3'","20                    20",59]
[2,"HMGR","HMG-CoA reductase","stevia5_contig_1202","Fwd 5'-AGATGCCGGTTGGCTATGTA-3'\nRev 5'-GTTCGTACTCGCCACCAAAC-3'","20                    20",59]
[3,"DXS","1-deoxy-D-xylulose-5-phosphate synthase","stevia5_contig_670","Fwd 5'-CAAATTGGACCTCAAGCACA-3'\nRev 5'-CGACGGGACCGATGTAGTAT-3'","20                    20",59]
[4,"GGPPS","Geranylgeranyl diphosphate synthase ","stevia5_contig_686","Fwd 5'-TCGTTACCACCGGCTAGAAG-3'\nRev 5'-GGGTCAGTTTGTGGACCTTG-3'","20                    20",59]
[5,"CPPS","Copalyl pyrophosphate synthase ","stevia5_contig_634","Fwd 5'-AGGCGTTGGTATTGAGGATG-3'\nRev 5'-TGGTTGACGAAATTGCAAGA-3'","22                    20",59]
[6,"KS","Ent-kaurene synthase ","stevia5_contig_3222","Fwd 5'-GGAATGTTGGGGAAGATCAA-3'\nRev 5'-ACCAATGGGAGATGGTTGAC-3'","20                    20",59]
[7,"KO","Ent-kaurene oxidase ","stevia5_contig_295","Fwd 5'-ACAAGCATTGGGAAAGGATG-3'\nRev 5'-CGGGTCAACAACCAATACCTA-3'","20                    20",59]
[8,"KA13H","Ent-kaurenoic acid 13-hydroxylase ","stevia5_contig_29139","Fwd 5'-CCTTCCATTTGGAGCAGGTA-3'\nRev 5'-TTGGATTGTCCCTTTCAAGC-3'","20                    20",59]
[9,"UGT 85C2","UDP-glycosyltransferase 85C2 ","stevia5_contig_742","Fwd 5'-TGGGAGAAGGAGGTCACAAA-3'\nRev 5'-CGGTGATTTCCTTGACCATT-3'","20                    20",59]
[10,"UGT 74G1","UDP-glycosyltransferase 74G1 ","stevia5_contig_9152","Fwd 5'-GGAAAGGAGGGTTTGGTTGT-3'\nRev 5'-GGCACTCCCAGACTTATTGC-3'","20                    20",59]
[11,"UGT 76G1","UDP-glycosyltransferase 76G1","stevia5_contig_14123","Fwd 5'-ACTTGGCGGTTGTTGGTCTA-3'\nRev 5'-GTGACTTCCTCCCGACACC-3'","20                    20",59]
[12,"KAO","Ent-kaurenoic acid oxidase ","stevia5_contig_26532","Fwd 5'-TATGCGGGCTATACCATTCC-3'\nRev 5'-CCTTCGAACCTTGATGGATT-3'","20                    20",59]
[13,"GA20-Ox","Gibberellin 20-oxidase ","stevia5_contig_33990","Fwd 5'-CGTCCCTAAGGTAAGGTCTGG-3'\nRev 5'-TTACTTGGGATGAGCCTTGG-3'","21                    20",59]
[14,"GA3-Ox","Gibberellin 3-oxidase ","stevia5_contig_19030","Fwd 5'-GCACTTGTGGTCAATGTTGG-3'\nRev 5'-AAGATAAGCGACGGACAACC-3'","20                   20",59]
[15,"GA2-Ox","Gibberellin 2-oxidase","stevia5_contig_26244","Fwd 5'-TGGATTCGGAGAACACACAG-3'\nRev 5'-TTGGAATCAGGAGGAACTGG-3'","20                    20",59]
[]
["Tm°C: melting temperature"]
["qRT-PCR conditions :"]
["The PCR amplification program was as follows: 95°C for 10 min; 35 cycles of 95°C for 30s, Tm°C for 30 sec and 72°C for 30s; followed by a melting-curve program of 95°C for 1min, 59°C for 30s and 95°C for 30s. "]