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["Domschke et al.: Systematic RNA-interference in primary human monocyte-derived macrophages identifies regulators of foam cell formation"]
["Supplemental Datasheet 1"]
["Results of primary RNAi-screening for functional regulators of DiI-LDL uptake into cultured primary human macrophages among 89 foam-cell regulated genes"]
["The impact on DiI-LDL uptake upon candidate gene knockdown was analyzed with 3 independent siRNAs/gene. SiRNAs meeting significance criteria (z-score/Deviation >|1|) are highlighted in red (increased LDL-uptake relative to control-siRNA treated cells) or green (reduced LDL-uptake relative to "]
["control-siRNA treated cells) as mean medians of deviations (Md). Negative values indicate reduction in DiI-LDL uptake. C, control individuals; P, coronary-artery disease patients. Column V [Md(C+P)] indicates means across patients and control individuals in this study. Column W [Md(C)-Md(P)] indicates difference in means between "]
["patients and controls in this study. *** met criteria for inclusion in validation screening; NA, not available."]
[]
["Symbol","Gene","Gene ID","siRNA ID","Sense siRNA Sequence","Antisense siRNA Sequence","Well","C 1","C 2","C 3","C 4","C 5","C 6","P 1","P 3","P 3","P 4","P 5","P 6","Md(C)","Md(P)","Md(C+P)","Md(C)-Md(P)"]
["a) 89 candidate genes analyzed in this study for a functional impact on foam cell induction"]
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["b) positive control siRNAs used for assay calibration"]
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["c) negative control siRNAs used for assay calibration"]
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["d) CXCR4 pathway genes analyzed during primary screening"]
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["CXCR7","chemokine (C-X-C motif) receptor 7","57007","s95","CCAUUAUCGCUGUCUUCUAtt","UAGAAGACAGCGAUAAUGGag","B3",{"v":-0.30110532395974365,"d":"-0.30"},{"v":-0.1512228053844679,"d":"-0.15"},{"v":-0.978478550038521,"d":"-0.98"},{"v":-0.08225855825670889,"d":"-0.08"},{"v":-0.6134010771172292,"d":"-0.61"},{"v":-0.8939522502054974,"d":"-0.89"},{"v":0.6478163765631766,"d":"0.65"},{"v":0.040247648060467184,"d":"0.04"},{"v":0.3440639124323029,"d":"0.34"},{"v":-1.8978498143254412,"d":"-1.90"},{"v":-1.3507692531294169,"d":"-1.35"},{"v":0.42601174858961754,"d":"0.43"},{"v":-0.45725320053848645,"d":"-0.46"},{"v":0.19215578024638505,"d":"0.19"},{"v":-0.22616406467210576,"d":"-0.23"},{"v":-0.6494089807848715,"d":"-0.65"}]
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{"sheet":"SupplData_Sheet2","index":1,"n_rows":58,"n_cols":36,"rows_kept":58,"merged":[[7,6,7,7],[8,0,8,35],[9,0,14,0],[9,1,14,1],[9,2,14,2],[9,3,11,3],[9,4,11,4],[9,5,11,5],[9,32,11,32],[9,33,11,33],[9,34,11,34],[9,35,11,35],[12,3,14,3],[12,4,14,4],[12,5,14,5],[12,32,14,32],[12,33,14,33],[12,34,14,34],[12,35,14,35],[15,0,20,0],[15,1,20,1],[15,2,20,2],[15,3,17,3],[15,4,17,4],[15,5,17,5],[15,32,17,32],[15,33,17,33],[15,34,17,34],[15,35,17,35],[18,3,20,3],[18,4,20,4],[18,5,20,5],[18,32,20,32],[18,33,20,33],[18,34,20,34],[18,35,20,35],[21,0,26,0],[21,1,26,1],[21,2,26,2],[21,3,23,3],[21,4,23,4],[21,5,23,5],[21,32,23,32],[21,33,23,33],[21,34,23,34],[21,35,23,35],[24,3,26,3],[24,4,26,4],[24,5,26,5],[24,32,26,32],[24,33,26,33],[24,34,26,34],[24,35,26,35],[27,0,32,0],[27,1,32,1],[27,2,32,2],[27,3,29,3],[27,4,29,4],[27,5,29,5],[27,32,29,32],[27,33,29,33],[27,34,29,34],[27,35,29,35],[30,3,32,3],[30,4,32,4],[30,5,32,5],[30,32,32,32],[30,33,32,33],[30,34,32,34],[30,35,32,35],[33,0,38,0],[33,1,38,1],[33,2,38,2],[33,3,35,3],[33,4,35,4],[33,5,35,5],[33,32,35,32],[33,33,35,33],[33,34,35,34],[33,35,35,35],[36,3,38,3],[36,4,38,4],[36,5,38,5],[36,32,38,32],[36,33,38,33],[36,34,38,34],[36,35,38,35],[39,0,44,0],[39,1,44,1],[39,2,44,2],[39,3,41,3],[39,4,41,4],[39,5,41,5],[39,32,41,32],[39,33,41,33],[39,34,41,34],[39,35,41,35],[42,3,44,3],[42,4,44,4],[42,5,44,5],[42,32,44,32],[42,33,44,33],[42,34,44,34],[42,35,44,35],[45,0,50,0],[45,1,50,1],[45,2,50,2],[45,3,47,3],[45,4,47,4],[45,5,47,5],[45,32,47,32],[45,33,47,33],[45,34,47,34],[45,35,47,35],[48,3,50,3],[48,4,50,4],[48,5,50,5],[48,32,50,32],[48,33,50,33],[48,34,50,34],[48,35,50,35],[51,0,51,35],[52,0,54,0],[52,1,54,1],[52,2,54,2],[52,3,54,3],[52,4,54,4],[52,5,54,5],[52,32,54,32],[52,33,54,33],[52,34,54,34],[52,35,54,35],[55,0,57,0],[55,1,57,1],[55,2,57,2],[55,3,57,3],[55,4,57,4],[55,5,57,5],[55,32,57,32],[55,33,57,33],[55,34,57,34],[55,35,57,35]],"flags":["formulas_cached_only"]}
["Domschke et al.: Systematic RNA-interference in primary human monocyte-derived macrophages identifies regulators of foam cell formation"]
["Supplemental Datasheet 2"]
["Results of validation RNAi-screening for functional regulators of DiI-LDL uptake into cultured primary human macrophages among 7 putative foam-cell regulators identified in primary screen"]
["For validation of primary screen results, the impact on DiI-LDL uptake upon knockdown of seven selected candidate genes was analyzed with 2 independent siRNAs/gene. SiRNAs meeting significance criteria (z-score/Deviation >|1|) are highlighted in green (reduced LDL-uptake relative to control-siRNA treated cells) as mean medians of deviations (Md). Negative values indicate reduction in DiI-LDL uptake. C, control individuals; "]
["P, coronary-artery disease patients. Columns AE and AI [Md(C+P)] indicate means across patients and control individuals in this study. Columns AF and AJ [Md(C)-Md(P)] indicate difference in means between patients and controls in this study. siRNAs with Md(C)-Md(P) >|1| are highligthed in red. Columns AG-AJ indicate meas for all measurements per siRNA during replication screen. "]
["Genes with 2 significant siRNAs validated in replication screen are highlighted by **.; NA, not available."]
[]
["Symbol","Gene","Gene ID","siRNA ID","Sense siRNA Sequence","Antisense siRNA Sequence","Wells",null,"C 1","C 2","C 3","C 4","C 5","C 6","C 7","C 8","C 9","C 10","P 1","P 2","P 3","P 4","P 5","P 6","P 7","P 8","P 9","P 10","Md(C)","Md(P)","Md(C+P)","Md(C)-Md(P)","Mean Md(C)","Mean Md(P)","Mean Md(C+P)","MMdC-MMdP"]
["a) 7 putative foam cell regulators nominated from primary screen"]
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