LOCUS Sequence 3456 bp DNA SYN DEFINITION Sequence ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers repeat_region 272..306 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" promoter complement(455..473) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(494..510) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(494..510) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" misc_feature 1..3756 /note="PB-TET" /dnas_title="CAGGAAACAGCTATGAC" /primer=CAGGAAACAGCTATGAC /note="M13-R" /set=Set 1 /dnas_title="CACACAGGAAACAGCTATGACCAT" /primer=CACACAGGAAACAGCTATGACCAT /note="M13_R" /set=Set 1 /dnas_title="GCGACGCGCGCTAAAGAATGG" /primer=GCGACGCGCGCTAAAGAATGG /note="PB-F" /set=Set 1 /dnas_title="CCAGGAAACAGCTATGACCAT" /primer=CCAGGAAACAGCTATGACCAT /note="M13-R-Petr" /set=Set 1 primer_bind complement(507..529) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 518..534 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(542..572) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 587..608 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(725..742) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(896..1484) /direction=ColE1/pMB1/pBR322/pUC origin /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(976..995) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1655..2515) /dnas_title="Amp" /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /dnas_title="GGGGACCACTTTGTACAAGAAAGCTGGGTGTGTCACGCTCGTCGT TTGGTATG" /primer=GGGGACCACTTTGTACAAGAAAGCTGGGTGTGTCACGCTCGTCGTTTGGT ATG /note="AttB2-SBP-Lib-R" /set=Set 1 /dnas_title="TGGCCCCAGTGCTGCAATGATAC" /primer=TGGCCCCAGTGCTGCAATGATAC /note="Biotin-Amp-R" /set=Set 1 /dnas_title="GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTGTCACGCTC GTCGTTTGGTATG" /primer=GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTGTCACGCTCGTCGT TTGGTATG /note="Read2-Amp-R" /set=Set 1 primer_bind 2278..2297 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" /dnas_title="TTGGGAACCGGAGCTGAATGAA" /primer=TTGGGAACCGGAGCTGAATGAA /note="Amp_F" /set=Set 1 /dnas_title="ATCATTGGAAAACGTTCTTC" /primer=ATCATTGGAAAACGTTCTTC /note="BGLII_MUT_VAL_F" /set=Set 1 promoter complement(2516..2620) /dnas_title="bla" /gene="bla" /label=AmpR promoter rep_origin complement(2649..3104) /direction=f1 bacteriophage origin of replication /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2786..2807) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2998..3017 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3245..3262 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3246..3262 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3269..3288 /label=T7 /note="T7 promoter, forward primer" promoter 3269..3287 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" repeat_region 3354..3416 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" /dnas_title="GTAAAACGACGGCCAG" /primer=GTAAAACGACGGCCAG /note="M13-F (-20)" /set=Set 1 /dnas_title="CGAGCTCGAATTCACTGGCC" /primer=CGAGCTCGAATTCACTGGCC /note="comp-20" /set=Set 1 /dnas_title="CGAGCTCGAATTCACTGGCC" /primer=CGAGCTCGAATTCACTGGCC /note="comp-20-bio" /set=Set 1 /dnas_title="GGGAACCGGCAGCATGGGCGCCGTCGTTTTACAACGTCGTGA" /primer=GGGAACCGGCAGCATGGGCGCCGTCGTTTTACAACGTCGTGA /note="HSPA1A-pr-Lacz-F" /set=Set 1 /dnas_title="GGGGAATTCACTGGCCGTCGTTTTACAA" /primer=GGGGAATTCACTGGCCGTCGTTTTACAA /note="Phage-EcoRI-F" /set=Set 1 /dnas_title="GTAAAACGACGGCCAGT" /primer=GTAAAACGACGGCCAGT /note="M13_F" /set=Set 1 /dnas_title="TAATACGACTCACTATAGGG" /primer=TAATACGACTCACTATAGGG /note="T7" /set=Set 1 /dnas_title="GATGTTTTCCCAGTCACGA" /primer=GATGTTTTCCCAGTCACGA /note="M13-F-Petr" /set=Set 1 misc_feature 3757..7511 /note="FP" misc_feature 3769..4952 /note="EF-1Alpha promoter" misc_feature 3942..3948 /note="TATA box" misc_feature 3970..3976 /note="INR" misc_feature 3972..3972 /note="+1" misc_feature 3972..4004 /note="Exon1" misc_feature 3998..4012 /note="Donor" /dnas_title="CGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTA" /primer=CGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTA /note="1stPCR-sgRNA-V2-F" /set=Set 1 /dnas_title="CGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTA" /primer=CGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTA /note="1stPCR-sgRNA-V2-R" /set=Set 1 /dnas_title="GGGGTTAATTAACGTAGCCCTCGTGAGGCTCCGGTGCCCGTC" /primer=GGGGTTAATTAACGTAGCCCTCGTGAGGCTCCGGTGCCCGTC /note="PacI-NheI-EF1-F" /set=Set 1 misc_feature 4005..4943 /note="Intron" misc_feature 4020..4024 /note="DPE (?)" /dnas_title="GAAGGAGATAGAACCATGCCGGAGGCCCCGCCTC" /primer=GAAGGAGATAGAACCATGCCGGAGGCCCCGCCTC /note="COMT-MB(CDS)-rbs-F" /set=Set 1 /dnas_title="GGCTCTGAGAACCTGTACTTCCAGAGCATGCCGGAGGCCCCGCCT C" /primer=GGCTCTGAGAACCTGTACTTCCAGAGCATGCCGGAGGCCCCGCCTC /note="COMT-MB(CDS)-TEV-F" /set=Set 1 /dnas_title="GGGAACCACACACGGCACTTAC" /primer=GGGAACCACACACGGCACTTAC /note="EF1-intron-R" /set=Set 1 misc_feature 4969..4988 /note="T7" misc_feature 4923..4963 /note="Acceptor" misc_feature 4944..5032 /note="Exon2" /dnas_title="AGGTGTCGTGAGGAATTAGC" /primer=AGGTGTCGTGAGGAATTAGC /note="FAM-T7-SBP-F" /set=Set 1 /dnas_title="GAAGGAGATAGAACCATGACCGAGCGCCGCGT" /primer=GAAGGAGATAGAACCATGACCGAGCGCCGCGT /note="HSPB1-RBS-F" /set=Set 1 /dnas_title="GGCTCTGAGAACCTGTACTTCCAGAGCATGACCGAGCGCCGCGT" /primer=GGCTCTGAGAACCTGTACTTCCAGAGCATGACCGAGCGCCGCGT /note="HSPB1-TEV-F" /set=Set 1 /dnas_title="TAATACGACTCACTATAGGG" /primer=TAATACGACTCACTATAGGG /note="T7" /set=Set 1 /dnas_title="CCTTGGAATTTGCCCTTTTTG" /primer=CCTTGGAATTTGCCCTTTTTG /note="EF1-intron-F" /set=Set 1 /dnas_title="GGGACTAGTCCAGTGTGAGGAGGAGTGGCACCATGGTGAGCAAGG GCGAGGAG" /primer=GGGACTAGTCCAGTGTGAGGAGGAGTGGCACCATGGTGAGCAAGGGCGAG GAG /note="SpeI-GFP-F" /set=Set 1 /dnas_title="GGCTCTGAGAACCTGTACTTCCAGAGCATGGTGAGCAAGGGCGAG GA" /primer=GGCTCTGAGAACCTGTACTTCCAGAGCATGGTGAGCAAGGGCGAGGA /note="TEV-FP-GW-F" /set=Set 1 /dnas_title="GATCCACTAGTCCAGTGTGAGGAGGAGTGGCACC" /primer=GATCCACTAGTCCAGTGTGAGGAGGAGTGGCACC /note="RBS-EFI-F" /set=Set 1 /dnas_title="GGTGCCACTCCTCCTCACACTGGACTAGTGGATC" /primer=GGTGCCACTCCTCCTCACACTGGACTAGTGGATC /note="RBS-EFI-R" /set=Set 1 enhancer 6914..7511 /note="WPRE" /dnas_title="CAAGTAATCTCGAGAATCAACCTCTGGATTACAAAATTTGTG" /primer=CAAGTAATCTCGAGAATCAACCTCTGGATTACAAAATTTGTG /note="Cherry-XhoI-WPRE-F" /set=Set 1 /dnas_title="CCAGAGGTTGATTGTCGATC" /primer=CCAGAGGTTGATTGTCGATC /note="pLenti za MluI R" /set=Set 1 /dnas_title="TTGGGCACTGACAATTCCGT" /primer=TTGGGCACTGACAATTCCGT /note="WPRE-F" /set=Set 1 /dnas_title="CAAAGGGAGATCCGACTCGT" /primer=CAAAGGGAGATCCGACTCGT /note="WPRE-R" /set=Set 1 RBS 5046..5051 /note="RBS" CDS 5061..5174 /dnas_title="SBP tag" /vntifkey=SBP /label=SBP tag primer_bind 5046..5083 /note="RBS-SBP primer F" primer_bind complement(5158..5191) /note="SBP-Att-R" primer_bind 5178..5191 /note="AttR1-F" misc_feature complement(5192..5208) /label=attL1 misc_feature 5589..6159 /note="IRES" misc_feature 5533..5545 /label=attL2 /translation="TQLSCTKLAL.ESIAYQFVATNRSLSVKIKSLF" CDS 6168..6887 /note="EGFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" primer_bind 6136..6184 /note="IRES-EGFP-F" primer_bind complement(6136..6184) /note="IRES-EGFP-R" primer_bind complement(6866..6898) /note="EGFP-MluI-R" promoter 7522..7830 /note="SV40 promoter" promoter 7885..7951 /note="EM7 promoter" gene 7952..8350 /note="Blasticidin resistance gene" LTR 8436..8670 /note="deltaU3-3' LTR" LTR 8436..8489 /note="delta U3" LTR 8490..8670 /note="3'LTR" polyA_signal 8742..8873 /note="SV40 polyadenylation signal" promoter 9732..9830 /note="bla promoter" gene 9831..10691 /note="Ampicillin (bla) resistance gene" rep_origin 10836..11509 /note="pUC origin" CDS 5226..5531 /note="SUMO1" /translation="MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHL KKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV" BASE COUNT 2935 a 3006 c 3004 g 2971 t ORIGIN 1 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca 61 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt 121 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc 181 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta 241 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt 301 ctagggttaa aaaagatttg cgctttactc gacctaaact ttaaacacgt catagaatct 361 tcgtttgaca aaaaccacat tgtggccaag ctgtgtgacg cgacgcgcgc taaagaatgg 421 caaaccaagt cgcgcgaggt accagctttt gttcccttta gtgagggtta atttcgagct 481 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 541 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 601 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 661 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 721 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 781 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 841 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 1021 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 1081 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 1141 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 1201 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 1261 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 1321 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 1381 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 1441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 1501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 1561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 1621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 1681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 1741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 1801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 1861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 1921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 1981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 2041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 2101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 2161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 2221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 2281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 2341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 2401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 2461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 2521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 2581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 2641 cacctgggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat 2701 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata 2761 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt 2821 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc 2881 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa 2941 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg 3001 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt 3061 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag 3121 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 3181 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 3241 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattggagct 3301 cggtattcac gacagcaggc tgaataataa aaaaattaga aactattatt taaccctaga 3361 aagataatca tattgtgacg tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt 3421 ttatcggtct gtatatcgag gtttatttat taatttgaat agatattaag ttttattata 3481 tttacactta catactaata ataaattcaa caaacaattt atttatgttt atttatttat 3541 taaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt aaacattctc 3601 tcttttacaa aaataaactt attttgtact ttaaaaacag tcatgttgta ttataaaata 3661 agtaattagc ttaacctata cataatagaa acaaattata cttattagtc agtcagaaac 3721 aactttggca catatcaata ttatgctctc gttaatTAAc gtagcCCTCG TGAGGCTCCG 3781 GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC CGAGAAGTTG GGGGGAGGGG 3841 TCGGCAATTG AACCGGTGCC TAGAGAAGGT GGCGCGGGGT AAACTGGGAA AGTGATGTCG 3901 TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC GTATATAAGT GCAGTAGTCG 3961 CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC ACAGGTAAGT GCCGTGTGTG 4021 GTTCCCGCGG GCCTGGCCTC TTTACGGGTT ATGGCCCTTG CGTGCCTTGA ATTACTTCCA 4081 CCTGGCTGCA GTACGTGATT CTTGATCCCG AGCTTCGGGT TGGAAGTGGG TGGGAGAGTT 4141 CGAGGCCTTG CGCTTAAGGA GCCCCTTCGC CTCGTGCTTG AGTTGAGGCC TGGCCTGGGC 4201 GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC ACCTTCGCGC CTGTCTCGCT GCTTTCGATA 4261 AGTCTCTAGC CATTTAAAAT TTTTGATGAC CTGCTGCGAC GCTTTTTTTC TGGCAAGATA 4321 GTCTTGTAAA TGCGGGCCAA GATCTGCACA CTGGTATTTC GGTTTTTGGG GCCGCGGGCG 4381 GCGACGGGGC CCGTGCGTCC CAGCGCACAT GTTCGGCGAG GCGGGGCCTG CGAGCGCGGC 4441 CACCGAGAAT CGGACGGGGG TAGTCTCAAG CTGGCCGGCC TGCTCTGGTG CCTGGCCTCG 4501 CGCCGCCGTG TATCGCCCCG CCCTGGGCGG CAAGGCTGGC CCGGTCGGCA CCAGTTGCGT 4561 GAGCGGAAAG ATGGCCGCTT CCCGGCCCTG CTGCAGGGAG CTCAAAATGG AGGACGCGGC 4621 GCTCGGGAGA GCGGGCGGGT GAGTCACCCA CACAAAGGAA AAGGGCCTTT CCGTCCTCAG 4681 CCGTCGCTTC ATGTGACTCC ACGGAGTACC GGGCGCCGTC CAGGCACCTC GATTAGTTCT 4741 CGAGCTTTTG GAGTACGTCG TCTTTAGGTT GGGGGGAGGG GTTTTATGCG ATGGAGTTTC 4801 CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG GCACTTGATG TAATTCTCCT 4861 TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC 4921 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTAG CTTGGTACTA ATACGACTCA 4981 CTATAGGGAG ACCCAAGCTG GCTAGGTAAG CTTGGTACCG AGCTCGGATC CACTAGTCCA 5041 GTGTGAGGAG GAGTGGCACC atggacgaaa aaaccaccgg ttggcgtggt ggtcacgttg 5101 ttgaaggtct ggctggtgaa ctggaacagc tgcgtgctcg tctggaacac cacccgcagg 5161 gtcagcgtga acccagcGGC AGCACAAGTT TGTACAAAAA AGCAGGCTTC GAAGGAGATA 5221 GAACCatgtc tgaccaggag gcaaaacctt caactgagga cttgggggat aagaaggaag 5281 gtgaatatat taaactcaaa gtcattggac aggatagcag tgagattcac ttcaaagtga 5341 aaatgacaac acatctcaag aaactcaaag aatcatactg tcaaagacag ggtgttccaa 5401 tgaattcact caggtttctc tttgagggtc agagaattgc tgataatcat actccaaaag 5461 aactgggaat ggaggaagaa gatgtgattg aagtttatca ggaacaaacg gggggtcatt 5521 caacagttta gAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCCAGCAC AGTGGCGGCC 5581 GCTCGAGTCC CCCTCTCCCT CCCCCCCCCC TAACGTTACT GGCCGAAGCC GCTTGGAATA 5641 AGGCCGGTGT GCGTTTGTCT ATATGTTATT TTCCACCATA TTGCCGTCTT TTGGCAATGT 5701 GAGGGCCCGG AAACCTGGCC CTGTCTTCTT GACGAGCATT CCTAGGGGTC TTTCCCCTCT 5761 CGCCAAAGGA ATGCAAGGTC TGTTGAATGT CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC 5821 TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG CGGAACCCCC CACCTGGCGA 5881 CAGGTGCCTC TGCGGCCAAA AGCCACGTGT ATAAGATACA CCTGCAAAGG CGGCACAACC 5941 CCAGTGCCAC GTTGTGAGTT GGATAGTTGT GGAAAGAGTC AAATGGCTCT CCTCAAGCGT 6001 ATTCAACAAG GGGCTGAAGG ATGCCCAGAA GGTACCCCAT TGTATGGGAT CTGATCTGGG 6061 GCCTCGGTGC ACATGCTTTA CATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG 6121 AACCACGGGG ACGTGGTTTT CCTTTGAAAA ACACGATCGT TAAAACCATG GTGAGCAAGG 6181 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG 6241 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC 6301 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCT 6361 TCACCTACGG CGTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT 6421 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG 6481 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG 6541 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA 6601 ACTACAACAG CCACAAGGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGTGA 6661 ACTTCAAGAC CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC 6721 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC 6781 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG 6841 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAATT CTACGCGTAC 6901 CGGTTAGTAA TGATCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 6961 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 7021 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 7081 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 7141 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 7201 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 7261 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG 7321 TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 7381 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 7441 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 7501 TCCCCGCCTG GCGATGGTAC CGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 7561 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG 7621 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA 7681 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC 7741 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT 7801 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT 7861 CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT 7921 AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG CCTTTGTCTC AAGAAGAATC 7981 CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG 8041 CGTCGCCAGC GCAGCTCTCT CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA 8101 TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC 8161 TGGCAACCTG ACTTGTATCG TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG 8221 CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG ATCAAAGCCA TAGTGAAGGA 8281 CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG 8341 GGAGGGCTAA GCACAATTCG AGCTCGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG 8401 TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC 8461 GAAGACAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT 8521 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG 8581 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC 8641 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT 8701 ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT 8761 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 8821 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC 8881 TATCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC 8941 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA 9001 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGGACGTACC CAATTCGCCC 9061 TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA 9121 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT 9181 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 9241 TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 9301 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 9361 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 9421 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 9481 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 9541 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 9601 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 9661 TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA 9721 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA 9781 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC 9841 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC 9901 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT 9961 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT 10021 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG 10081 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT 10141 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG 10201 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA 10261 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG 10321 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA 10381 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC 10441 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC 10501 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA 10561 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA 10621 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA 10681 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC 10741 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC 10801 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT 10861 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC 10921 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT 10981 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT 11041 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG 11101 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA 11161 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA 11221 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG 11281 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG 11341 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC 11401 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA 11461 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG 11521 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC 11581 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA 11641 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT 11701 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT 11761 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG 11821 GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC 11881 CTCACTAAAG GGAACAAAAG CTGGAGCTGC AAGCTT //