LOCUS Sequence 3456 bp DNA SYN DEFINITION Sequence ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers repeat_region 272..306 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" promoter complement(455..473) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(494..510) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(494..510) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" misc_feature 1..3756 /note="PB-TET" /dnas_title="CAGGAAACAGCTATGAC" /primer=CAGGAAACAGCTATGAC /note="M13-R" /set=Set 1 /dnas_title="CACACAGGAAACAGCTATGACCAT" /primer=CACACAGGAAACAGCTATGACCAT /note="M13_R" /set=Set 1 /dnas_title="GCGACGCGCGCTAAAGAATGG" /primer=GCGACGCGCGCTAAAGAATGG /note="PB-F" /set=Set 1 /dnas_title="CCAGGAAACAGCTATGACCAT" /primer=CCAGGAAACAGCTATGACCAT /note="M13-R-Petr" /set=Set 1 primer_bind complement(507..529) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 518..534 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(542..572) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 587..608 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(725..742) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(896..1484) /direction=ColE1/pMB1/pBR322/pUC origin /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(976..995) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1655..2515) /dnas_title="Amp" /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /dnas_title="GGGGACCACTTTGTACAAGAAAGCTGGGTGTGTCACGCTCGTCGT TTGGTATG" /primer=GGGGACCACTTTGTACAAGAAAGCTGGGTGTGTCACGCTCGTCGTTTGGT ATG /note="AttB2-SBP-Lib-R" /set=Set 1 /dnas_title="TGGCCCCAGTGCTGCAATGATAC" /primer=TGGCCCCAGTGCTGCAATGATAC /note="Biotin-Amp-R" /set=Set 1 /dnas_title="GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTGTCACGCTC GTCGTTTGGTATG" /primer=GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTGTCACGCTCGTCGT TTGGTATG /note="Read2-Amp-R" /set=Set 1 primer_bind 2278..2297 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" /dnas_title="TTGGGAACCGGAGCTGAATGAA" /primer=TTGGGAACCGGAGCTGAATGAA /note="Amp_F" /set=Set 1 /dnas_title="ATCATTGGAAAACGTTCTTC" /primer=ATCATTGGAAAACGTTCTTC /note="BGLII_MUT_VAL_F" /set=Set 1 promoter complement(2516..2620) /dnas_title="bla" /gene="bla" /label=AmpR promoter rep_origin complement(2649..3104) /direction=f1 bacteriophage origin of replication /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2786..2807) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2998..3017 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3245..3262 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3246..3262 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3269..3288 /label=T7 /note="T7 promoter, forward primer" promoter 3269..3287 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" repeat_region 3354..3416 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" /dnas_title="GTAAAACGACGGCCAG" /primer=GTAAAACGACGGCCAG /note="M13-F (-20)" /set=Set 1 /dnas_title="CGAGCTCGAATTCACTGGCC" /primer=CGAGCTCGAATTCACTGGCC /note="comp-20" /set=Set 1 /dnas_title="CGAGCTCGAATTCACTGGCC" /primer=CGAGCTCGAATTCACTGGCC /note="comp-20-bio" /set=Set 1 /dnas_title="GGGAACCGGCAGCATGGGCGCCGTCGTTTTACAACGTCGTGA" /primer=GGGAACCGGCAGCATGGGCGCCGTCGTTTTACAACGTCGTGA /note="HSPA1A-pr-Lacz-F" /set=Set 1 /dnas_title="GGGGAATTCACTGGCCGTCGTTTTACAA" /primer=GGGGAATTCACTGGCCGTCGTTTTACAA /note="Phage-EcoRI-F" /set=Set 1 /dnas_title="GTAAAACGACGGCCAGT" /primer=GTAAAACGACGGCCAGT /note="M13_F" /set=Set 1 /dnas_title="TAATACGACTCACTATAGGG" /primer=TAATACGACTCACTATAGGG /note="T7" /set=Set 1 /dnas_title="GATGTTTTCCCAGTCACGA" /primer=GATGTTTTCCCAGTCACGA /note="M13-F-Petr" /set=Set 1 misc_feature 3757..7493 /note="FP" misc_feature 3769..4952 /note="EF-1Alpha promoter" misc_feature 3942..3948 /note="TATA box" misc_feature 3970..3976 /note="INR" misc_feature 3972..3972 /note="+1" misc_feature 3972..4004 /note="Exon1" misc_feature 3998..4012 /note="Donor" /dnas_title="CGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTA" /primer=CGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTA /note="1stPCR-sgRNA-V2-F" /set=Set 1 /dnas_title="CGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTA" /primer=CGACATCACTTTCCCAGTTTACCCCGCGCCACCTTCTCTA /note="1stPCR-sgRNA-V2-R" /set=Set 1 /dnas_title="GGGGTTAATTAACGTAGCCCTCGTGAGGCTCCGGTGCCCGTC" /primer=GGGGTTAATTAACGTAGCCCTCGTGAGGCTCCGGTGCCCGTC /note="PacI-NheI-EF1-F" /set=Set 1 misc_feature 4005..4943 /note="Intron" misc_feature 4020..4024 /note="DPE (?)" /dnas_title="GAAGGAGATAGAACCATGCCGGAGGCCCCGCCTC" /primer=GAAGGAGATAGAACCATGCCGGAGGCCCCGCCTC /note="COMT-MB(CDS)-rbs-F" /set=Set 1 /dnas_title="GGCTCTGAGAACCTGTACTTCCAGAGCATGCCGGAGGCCCCGCCT C" /primer=GGCTCTGAGAACCTGTACTTCCAGAGCATGCCGGAGGCCCCGCCTC /note="COMT-MB(CDS)-TEV-F" /set=Set 1 /dnas_title="GGGAACCACACACGGCACTTAC" /primer=GGGAACCACACACGGCACTTAC /note="EF1-intron-R" /set=Set 1 misc_feature 4969..4988 /note="T7" misc_feature 4923..4963 /note="Acceptor" misc_feature 4944..5032 /note="Exon2" /dnas_title="AGGTGTCGTGAGGAATTAGC" /primer=AGGTGTCGTGAGGAATTAGC /note="FAM-T7-SBP-F" /set=Set 1 /dnas_title="GAAGGAGATAGAACCATGACCGAGCGCCGCGT" /primer=GAAGGAGATAGAACCATGACCGAGCGCCGCGT /note="HSPB1-RBS-F" /set=Set 1 /dnas_title="GGCTCTGAGAACCTGTACTTCCAGAGCATGACCGAGCGCCGCGT" /primer=GGCTCTGAGAACCTGTACTTCCAGAGCATGACCGAGCGCCGCGT /note="HSPB1-TEV-F" /set=Set 1 /dnas_title="TAATACGACTCACTATAGGG" /primer=TAATACGACTCACTATAGGG /note="T7" /set=Set 1 /dnas_title="CCTTGGAATTTGCCCTTTTTG" /primer=CCTTGGAATTTGCCCTTTTTG /note="EF1-intron-F" /set=Set 1 /dnas_title="GGGACTAGTCCAGTGTGAGGAGGAGTGGCACCATGGTGAGCAAGG GCGAGGAG" /primer=GGGACTAGTCCAGTGTGAGGAGGAGTGGCACCATGGTGAGCAAGGGCGAG GAG /note="SpeI-GFP-F" /set=Set 1 /dnas_title="GGCTCTGAGAACCTGTACTTCCAGAGCATGGTGAGCAAGGGCGAG GA" /primer=GGCTCTGAGAACCTGTACTTCCAGAGCATGGTGAGCAAGGGCGAGGA /note="TEV-FP-GW-F" /set=Set 1 /dnas_title="GATCCACTAGTCCAGTGTGAGGAGGAGTGGCACC" /primer=GATCCACTAGTCCAGTGTGAGGAGGAGTGGCACC /note="RBS-EFI-F" /set=Set 1 /dnas_title="GGTGCCACTCCTCCTCACACTGGACTAGTGGATC" /primer=GGTGCCACTCCTCCTCACACTGGACTAGTGGATC /note="RBS-EFI-R" /set=Set 1 enhancer 6896..7493 /note="WPRE" /dnas_title="CAAGTAATCTCGAGAATCAACCTCTGGATTACAAAATTTGTG" /primer=CAAGTAATCTCGAGAATCAACCTCTGGATTACAAAATTTGTG /note="Cherry-XhoI-WPRE-F" /set=Set 1 /dnas_title="CCAGAGGTTGATTGTCGATC" /primer=CCAGAGGTTGATTGTCGATC /note="pLenti za MluI R" /set=Set 1 /dnas_title="TTGGGCACTGACAATTCCGT" /primer=TTGGGCACTGACAATTCCGT /note="WPRE-F" /set=Set 1 /dnas_title="CAAAGGGAGATCCGACTCGT" /primer=CAAAGGGAGATCCGACTCGT /note="WPRE-R" /set=Set 1 RBS 5046..5051 /note="RBS" CDS 5061..5174 /dnas_title="SBP tag" /vntifkey=SBP /label=SBP tag primer_bind 5046..5083 /note="RBS-SBP primer F" primer_bind complement(5158..5191) /note="SBP-Att-R" primer_bind 5178..5191 /note="AttR1-F" CDS 5226..5513 /dnas_title="SUMO2" /gene="SUMO2" /gene_synonym=HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3 /note="isoform a precursor is encoded by transcript variant 1; sentrin 2; SMT3 suppressor of mif two 3 homolog 2; SMT3 homolog 2; ubiquitin-like protein SMT3B; ubiquitin-like protein SMT3A" /codon_start=1 /product="small ubiquitin-related modifier 2 isoform a precursor" /protein_id="NP_008868.3" /db_xref="CCDS:CCDS45774.1" /db_xref="GeneID:6613" /db_xref="HGNC:HGNC:11125" /db_xref="MIM:603042" /translation="MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLM KAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVY" mat_peptide 5226..5504 /dnas_title="SUMO2" /gene="SUMO2" /gene_synonym=HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3 /product="small ubiquitin-related modifier 2 isoform a" misc_feature 5256..5258 /dnas_title="SUMO2" /gene="SUMO2" /gene_synonym=HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3 /experiment=experimental evidence, no additional details recorded /note="N6-acetyllysine, alternate. {ECO:0000244|PubMed:19608861}; propagated from UniProtKB/Swiss-Prot (P61956.3); acetylation site" exon 5247..5378 /dnas_title="SUMO2" /gene="SUMO2" /gene_synonym=HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3 /inference=alignment:Splign:1.39.8 exon 5379..5450 /dnas_title="SUMO2" /gene="SUMO2" /gene_synonym=HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3 /inference=alignment:Splign:1.39.8 /dnas_title="TCAGTAGACACCTCCCGTCTG" /pair /primer=TCAGTAGACACCTCCCGTCTG /current=0 /dnas_title="GTAGACACCTCCCGTCTGCTG" /pair /primer=GTAGACACCTCCCGTCTGCTG /current=1 exon 5226..5246 /dnas_title="SUMO2" /gene="SUMO2" /gene_synonym=HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3 /inference=alignment:Splign:1.39.8 STS 5397..5513 /dnas_title="SUMO2" /gene="SUMO2" /gene_synonym=HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3 /standard_name="RH47248" /db_xref="UniSTS:85678" exon 5451..5513 /dnas_title="SUMO2" /gene="SUMO2" /gene_synonym=HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3 /inference=alignment:Splign:1.39.8 misc_feature complement(5192..5208) /label=attL1 misc_feature 5571..6141 /note="IRES" misc_feature 5515..5527 /label=attL2 /translation="TQLSCTKLAL.ESIAYQFVATNRSLSVKIKSLF" CDS 6150..6869 /note="EGFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" primer_bind 6118..6166 /note="IRES-EGFP-F" primer_bind complement(6118..6166) /note="IRES-EGFP-R" primer_bind complement(6848..6880) /note="EGFP-MluI-R" promoter 7504..7812 /note="SV40 promoter" promoter 7867..7933 /note="EM7 promoter" gene 7934..8332 /note="Blasticidin resistance gene" LTR 8418..8652 /note="deltaU3-3' LTR" LTR 8418..8471 /note="delta U3" LTR 8472..8652 /note="3'LTR" polyA_signal 8724..8855 /note="SV40 polyadenylation signal" promoter 9714..9812 /note="bla promoter" gene 9813..10673 /note="Ampicillin (bla) resistance gene" rep_origin 10818..11491 /note="pUC origin" BASE COUNT 2921 a 3008 c 3009 g 2960 t ORIGIN 1 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca 61 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt 121 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc 181 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta 241 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt 301 ctagggttaa aaaagatttg cgctttactc gacctaaact ttaaacacgt catagaatct 361 tcgtttgaca aaaaccacat tgtggccaag ctgtgtgacg cgacgcgcgc taaagaatgg 421 caaaccaagt cgcgcgaggt accagctttt gttcccttta gtgagggtta atttcgagct 481 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 541 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 601 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 661 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 721 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 781 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 841 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 1021 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 1081 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 1141 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 1201 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 1261 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 1321 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 1381 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 1441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 1501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 1561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 1621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 1681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 1741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 1801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 1861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 1921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 1981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 2041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 2101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 2161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 2221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 2281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 2341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 2401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 2461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 2521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 2581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 2641 cacctgggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat 2701 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata 2761 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt 2821 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc 2881 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa 2941 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg 3001 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt 3061 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag 3121 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 3181 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 3241 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattggagct 3301 cggtattcac gacagcaggc tgaataataa aaaaattaga aactattatt taaccctaga 3361 aagataatca tattgtgacg tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt 3421 ttatcggtct gtatatcgag gtttatttat taatttgaat agatattaag ttttattata 3481 tttacactta catactaata ataaattcaa caaacaattt atttatgttt atttatttat 3541 taaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt aaacattctc 3601 tcttttacaa aaataaactt attttgtact ttaaaaacag tcatgttgta ttataaaata 3661 agtaattagc ttaacctata cataatagaa acaaattata cttattagtc agtcagaaac 3721 aactttggca catatcaata ttatgctctc gttaatTAAc gtagcCCTCG TGAGGCTCCG 3781 GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC CGAGAAGTTG GGGGGAGGGG 3841 TCGGCAATTG AACCGGTGCC TAGAGAAGGT GGCGCGGGGT AAACTGGGAA AGTGATGTCG 3901 TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC GTATATAAGT GCAGTAGTCG 3961 CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC ACAGGTAAGT GCCGTGTGTG 4021 GTTCCCGCGG GCCTGGCCTC TTTACGGGTT ATGGCCCTTG CGTGCCTTGA ATTACTTCCA 4081 CCTGGCTGCA GTACGTGATT CTTGATCCCG AGCTTCGGGT TGGAAGTGGG TGGGAGAGTT 4141 CGAGGCCTTG CGCTTAAGGA GCCCCTTCGC CTCGTGCTTG AGTTGAGGCC TGGCCTGGGC 4201 GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC ACCTTCGCGC CTGTCTCGCT GCTTTCGATA 4261 AGTCTCTAGC CATTTAAAAT TTTTGATGAC CTGCTGCGAC GCTTTTTTTC TGGCAAGATA 4321 GTCTTGTAAA TGCGGGCCAA GATCTGCACA CTGGTATTTC GGTTTTTGGG GCCGCGGGCG 4381 GCGACGGGGC CCGTGCGTCC CAGCGCACAT GTTCGGCGAG GCGGGGCCTG CGAGCGCGGC 4441 CACCGAGAAT CGGACGGGGG TAGTCTCAAG CTGGCCGGCC TGCTCTGGTG CCTGGCCTCG 4501 CGCCGCCGTG TATCGCCCCG CCCTGGGCGG CAAGGCTGGC CCGGTCGGCA CCAGTTGCGT 4561 GAGCGGAAAG ATGGCCGCTT CCCGGCCCTG CTGCAGGGAG CTCAAAATGG AGGACGCGGC 4621 GCTCGGGAGA GCGGGCGGGT GAGTCACCCA CACAAAGGAA AAGGGCCTTT CCGTCCTCAG 4681 CCGTCGCTTC ATGTGACTCC ACGGAGTACC GGGCGCCGTC CAGGCACCTC GATTAGTTCT 4741 CGAGCTTTTG GAGTACGTCG TCTTTAGGTT GGGGGGAGGG GTTTTATGCG ATGGAGTTTC 4801 CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG GCACTTGATG TAATTCTCCT 4861 TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC 4921 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTAG CTTGGTACTA ATACGACTCA 4981 CTATAGGGAG ACCCAAGCTG GCTAGGTAAG CTTGGTACCG AGCTCGGATC CACTAGTCCA 5041 GTGTGAGGAG GAGTGGCACC atggacgaaa aaaccaccgg ttggcgtggt ggtcacgttg 5101 ttgaaggtct ggctggtgaa ctggaacagc tgcgtgctcg tctggaacac cacccgcagg 5161 gtcagcgtga acccagcGGC AGCACAAGTT TGTACAAAAA AGCAGGCTTC GAAGGAGATA 5221 GAACCatggc cgacgaaaag cccaaggaag gagtcaagac tgagaacaac gatcatatta 5281 atttgaaggt ggcggggcag gatggttctg tggtgcagtt taagattaag aggcatacac 5341 cacttagtaa actaatgaaa gcctattgtg aacgacaggg attgtcaatg aggcagatca 5401 gattccgatt tgacgggcaa ccaatcaatg aaacagacac acctgcacag ttggaaatgg 5461 aggatgaaga tacaattgat gtgttccaac agcagacggg aggtgtctac tgaAACCCAG 5521 CTTTCTTGTA CAAAGTGGTT GATATCCAGC ACAGTGGCGG CCGCTCGAGT CCCCCTCTCC 5581 CTCCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT 5641 CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG 5701 CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG 5761 TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC 5821 TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA 5881 AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG 5941 TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA AGGGGCTGAA 6001 GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT GCACATGCTT 6061 TACATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG GGACGTGGTT 6121 TTCCTTTGAA AAACACGATC GTTAAAACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG 6181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT 6241 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA 6301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CTTCACCTAC GGCGTGCAGT 6361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG 6421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG 6481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT 6541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAAGG 6601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ACCCGCCACA 6661 ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG 6721 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG 6781 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA 6841 CTCTCGGCAT GGACGAGCTG TACAAGTAAA TTCTACGCGT ACCGGTTAGT AATGATCGAC 6901 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 6961 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 7021 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 7081 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 7141 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 7201 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 7261 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC 7321 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 7381 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 7441 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGCGATGGT 7501 ACCGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 7561 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 7621 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 7681 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 7741 CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 7801 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC 7861 AGCACGTGTT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT 7921 GAGGAACTAA ACCATGGCCA AGCCTTTGTC TCAAGAAGAA TCCACCCTCA TTGAAAGAGC 7981 AACGGCTACA ATCAACAGCA TCCCCATCTC TGAAGACTAC AGCGTCGCCA GCGCAGCTCT 8041 CTCTAGCGAC GGCCGCATCT TCACTGGTGT CAATGTATAT CATTTTACTG GGGGACCTTG 8101 TGCAGAACTC GTGGTGCTGG GCACTGCTGC TGCTGCGGCA GCTGGCAACC TGACTTGTAT 8161 CGTCGCGATC GGAAATGAGA ACAGGGGCAT CTTGAGCCCC TGCGGACGGT GCCGACAGGT 8221 GCTTCTCGAT CTGCATCCTG GGATCAAAGC CATAGTGAAG GACAGTGATG GACAGCCGAC 8281 GGCAGTTGGG ATTCGTGAAT TGCTGCCCTC TGGTTATGTG TGGGAGGGCT AAGCACAATT 8341 CGAGCTCGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT 8401 TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATCTGCTTT 8461 TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC 8521 TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG 8581 CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA 8641 AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA 8701 AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 8761 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 8821 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC 8881 GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT 8941 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG 9001 AGGAGGCTTT TTTGGAGGCC TAGGGACGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC 9061 GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA 9121 CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC 9181 ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGACGC GCCCTGTAGC 9241 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 9301 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 9361 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 9421 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 9481 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 9541 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 9601 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC 9661 AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA 9721 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT 9781 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC 9841 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA 9901 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA 9961 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT 10021 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG 10081 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC 10141 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA 10201 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT 10261 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 10321 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA 10381 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG 10441 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG 10501 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG 10561 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 10621 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG 10681 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA 10741 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT 10801 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC 10861 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC 10921 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC 10981 CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC 11041 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT 11101 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT 11161 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT 11221 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT 11281 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG 11341 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT 11401 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 11461 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG 11521 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG 11581 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC 11641 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG 11701 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC 11761 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG 11821 GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA 11881 AGCTGGAGCT GCAAGCTT //