Table S4. Alpha diversity per sample														
														
seq_ID	Sample	Group	Type	depth_rarefied	Observed	Chao1	Chao1_se	ACE	ACE_se	Shannon	Simpson	Simpson_dominance	InvSimpson	Pielou_evenness
IG-2P	1st_BFP	1st	AMD precipitate-associated biofilm	88397	344	345.3333333	1.560185746	346.3410582	9.112381589	3.553611315	0.927711645	0.072288355	13.83348682	0.608428238
IG-2PW	1st_BFW1	1st	wall-attached biofilm	88397	225	225.1666667	0.475348753	225.9027008	7.217333301	3.409474871	0.938137414	0.061862586	16.16485931	0.629507324
IG-W2	1st_BFW2	1st	wall-attached biofilm	88397	250	250.5882353	0.978573697	251.5156341	7.701371479	3.041720185	0.881006745	0.118993255	8.403837669	0.550890467
IG-1P	2nd_BFP	2nd	AMD precipitate-associated biofilm	88397	175	175	0	175	5.78174467	2.563837605	0.812021723	0.187978277	5.319763621	0.496407328
IG-W1	2nd_BFW	2nd	wall-attached biofilm	88397	217	217.0909091	0.358799222	217.6011159	6.222544654	3.328217257	0.91895784	0.08104216	12.33925644	0.618639546
IG-WAT-1	2nd_wt	2nd	water	88397	293	293.5882353	0.978583552	294.3092158	8.374347727	2.710576831	0.830491248	0.169508752	5.899400387	0.477199729
IG-WAT-R	3rd_wt	3rd	water	88397	251	251	0	251	7.616296221	2.386144537	0.775973644	0.224026356	4.463760505	0.431845961
														
														
Exploratory Kruskal-Wallis by Type (n=2/3/2 - underpowered; not a confirmatory test)														
Metric	Kruskal-Wallis chi-sq	df	P-value											
Observed	1.607142857	2	0.44772708											
Chao1	1.607142857	2	0.44772708											
ACE	0.714285714	2	0.699672537											
Shannon	2.464285714	2	0.291666907											
Simpson	2.857142857	2	0.239651036											
InvSimpson	2.857142857	2	0.239651036											
Pielou_evenness	4.464285714	2	0.107298259