Table S3. Genus-level Levins' niche breadth and aggregation sensitivity "Analysis basis: 716,915 reads assigned to 663 ASVs across seven samples; 129 named genera were retained without an abundance or prevalence eligibility filter. Genus, Kingdom, and Phylum labels were taken from the accepted taxa_silva144.csv formal taxonomy table." "Primary calculation: genus counts were converted to sample relative sequence abundances, then averaged within each microhabitat Type with equal weight for every sample. Levins' B and the linearly standardized B_A are continuous descriptive measures across three Type states." "Sensitivity calculation: genus counts were pooled within each Type before calculating B and B_A. This gives more weight to Types with more samples and to samples with greater read depth. No generalist, neutral/intermediate, or specialist category was assigned." Method references: https://doi.org/10.1515/9780691209418; https://doi.org/10.2307/1936632; https://doi.org/10.1093/femsec/fiaa131 Genus Kingdom Phylum Total reads Samples detected (of 7) Mean relative abundance across samples (%) Type mean: AMD precipitate-associated biofilm (%) Type mean: wall-attached biofilm (%) Type mean: water (%) Levins B: equal-weighted Type means (primary) Standardized B_A: equal-weighted Type means (primary) Levins B: pooled reads within Type (sensitivity) Standardized B_A: pooled reads within Type (sensitivity) Absolute difference in standardized B_A Metallibacterium Bacteria Pseudomonadota 105266 7 14.14495175 10.16743991 25.60154838 0.937568657 1.773568418 0.386784209 1.566434346 0.283217173 0.103567036 Leptospirillum Bacteria Nitrospirota 63493 7 8.615301185 9.54259945 11.24710488 3.740297378 2.598683436 0.799341718 2.294677645 0.647338823 0.152002896 Acidibacillus--other Bacteria Bacillota--other 24135 6 3.483918816 10.462547 1.005086419 0.223539232 1.236674472 0.118337236 1.347370217 0.173685109 0.055347873 Cuniculiplasma Archaea Thermoplasmatota 18885 7 2.642951809 1.198444853 3.696116975 2.507711017 2.562106974 0.781053487 2.205635744 0.602817872 0.178235615 Acidibacter Bacteria Pseudomonadota 17301 7 2.323731628 1.514969572 4.275029742 0.205546515 1.74385649 0.371928245 1.549229855 0.274614927 0.097313318 Aciditerrimonas Bacteria Actinomycetota 12232 7 1.67090082 1.764468502 1.913327671 1.213692861 2.901178639 0.950589319 2.622908444 0.811454222 0.139135098 Thiomonas Bacteria Pseudomonadota 8625 7 1.21178723 1.714567386 1.436094052 0.37254684 2.414557755 0.707278877 2.300798215 0.650399107 0.05687977 Acidithiobacillus Bacteria Pseudomonadota 7748 7 1.051281059 1.307489904 1.30291011 0.417628636 2.560075584 0.780037792 2.369885904 0.684942952 0.09509484 Acidiphilium Bacteria Pseudomonadota 5961 7 0.844665527 0.114285403 1.865649591 0.043569554 1.171347252 0.085673626 1.1174675 0.05873375 0.026939876 RCP1-48 Bacteria Pseudomonadota 5296 7 0.736139489 0.319412504 1.367390919 0.205989328 1.778691151 0.389345576 1.525018519 0.26250926 0.126836316 Ferrithrix Bacteria Actinomycetota 3698 7 0.493605871 0.283734754 0.92485498 0.056603324 1.704580227 0.352290114 1.497267295 0.248633648 0.103656466 Acidithrix Bacteria Actinomycetota 3073 7 0.422775993 0.713107976 0.416912209 0.141239687 2.301196924 0.650598462 2.352252995 0.676126498 0.025528036 Candidatus Parvarchaeum Archaea Nanobdellota 2958 7 0.422607745 0.061299705 0.066418873 1.318199094 1.197534879 0.098767439 1.259105594 0.129552797 0.030785357 Acidiferrimicrobium Bacteria Actinomycetota 2511 7 0.34519334 0.193571025 0.657778486 0.027937935 1.641768891 0.320884445 1.447655016 0.223827508 0.097056937 Conexivisphaera Archaea Thermoproteota 1898 7 0.268281799 0.132469835 0.07400121 0.695514645 1.605434654 0.302717327 1.724361024 0.362180512 0.059463185 Ferroplasma Archaea Thermoplasmatota 1490 6 0.207405682 0.029727425 0.436103811 0.042036746 1.337551559 0.168775779 1.221659599 0.1108298 0.05794598 Legionella Bacteria Pseudomonadota 1285 6 0.181386677 0.01996375 0.40557182 0.006531892 1.131889957 0.065944979 1.088287575 0.044143787 0.021801191 Acidicapsa Bacteria Acidobacteriota 1286 7 0.177903545 0.216359179 0.174299027 0.144854688 2.921069816 0.960534908 2.803471716 0.901735858 0.05879905 Nitrosotalea Archaea Thermoproteota 1220 6 0.175430151 0.03226714 0.028574676 0.538876376 1.230684466 0.115342233 1.293691645 0.146845823 0.03150359 Candidatus Cybelea Bacteria Vulcanimicrobiota 1279 7 0.165586222 0.276145583 0.182107189 0.030245412 2.162797065 0.581398532 2.178875639 0.589437819 0.008039287 Granulicella Bacteria Acidobacteriota 1262 6 0.157895838 0.43207976 0.05881444 0.032334012 1.431858138 0.215929069 1.501695324 0.250847662 0.034918593 Acidibrevibacterium Bacteria Pseudomonadota 923 7 0.12863389 0.249264979 0.119205334 0.022145637 1.985866543 0.492933272 2.138241827 0.569120914 0.076187642 Desulfosporosinus Bacteria Desulfotomaculota 906 3 0.11087541 0.337151263 0.032127367 0.002721622 1.206377168 0.103188584 1.281479007 0.140739503 0.037550919 Candidatus Obscuribacter Bacteria Cyanobacteriota 724 7 0.101990112 0.144825679 0.080103539 0.091984405 2.801342356 0.900671178 2.874749092 0.937374546 0.036703368 Ferritrophicum Bacteria Pseudomonadota 718 5 0.101207627 0.019679061 0.009816696 0.319822591 1.187342246 0.093671123 1.234770279 0.117385139 0.023714017 Clostridium--other Bacteria Clostridiota 762 4 0.093142618 0.290383749 0.022785139 0.001437704 1.166580604 0.083290302 1.229464252 0.114732126 0.031441824 Acidiferrobacter Bacteria Pseudomonadota 593 6 0.083446154 0.025912232 0.054333956 0.184648374 1.860330078 0.430165039 2.096305746 0.548152873 0.117987834 Rhodanobacter Bacteria Pseudomonadota 541 3 0.066138309 0.212154452 0.010709122 0.003265946 1.132928214 0.066464107 1.16916667 0.084583335 0.018119228 Candidatus Captivus Bacteria Pseudomonadota 434 5 0.060212648 0.036777194 0.115978049 0 1.576263164 0.288131582 1.35918603 0.179593015 0.108538567 Candidatus Micrarchaeum--other Archaea Candidatus Micrarchaeota 409 7 0.058617948 0.005238635 0.018348513 0.172401413 1.276708708 0.138354354 1.3980744 0.1990372 0.060682846 Aquisphaera Bacteria Planctomycetota 413 6 0.054824953 0.107645806 0.045038008 0.016684519 2.064544092 0.532272046 2.201202751 0.600601376 0.06832933 Candidatus Acidulidesulfobacterium Bacteria Sva0485 367 4 0.052113306 0 0.017648628 0.15592363 1.223511778 0.111755889 1.33507459 0.167537295 0.055781406 WD260 Bacteria Pseudomonadota 353 3 0.043187007 0.133074837 0.009816696 0.003354643 1.200454852 0.100227426 1.257317848 0.128658924 0.028431498 Candidatus Berkiella Bacteria Pseudomonadota 296 6 0.040934885 0.038899243 0.062249375 0.010998792 2.282953748 0.641476874 1.885350318 0.442675159 0.198801715 Sulfurifustis Bacteria Pseudomonadota 273 7 0.037863379 0.034548394 0.033591248 0.047586561 2.920029836 0.960014918 2.934097083 0.967048541 0.007033623 Acidibacillus Bacteria Bacillota--other 268 7 0.037279412 0.037213593 0.047700487 0.021713617 2.751785166 0.875892583 2.35937192 0.67968596 0.196206623 JG30a-KF-32 Bacteria Chloroflexota--other 250 5 0.031597297 0.078927904 0.021108424 0 1.49917568 0.24958784 1.64568961 0.322844805 0.073256965 Trebonia Bacteria Actinomycetota 216 6 0.030353903 0.021797364 0.054842665 0.002177297 1.78122495 0.390612475 1.546129374 0.273064687 0.117547788 Sulfobacillus Bacteria Desulfotomaculota 188 7 0.025990111 0.010902429 0.048365994 0.007513969 1.77360294 0.38680147 1.519910553 0.259955277 0.126846193 Candidatus Chromulinivorax Bacteria Candidatus Babelota 176 7 0.025366712 0.03112464 0.031296017 0.010714826 2.592739291 0.796369646 2.357740904 0.678870452 0.117499194 Mycobacterium Bacteria Actinomycetota 173 6 0.023796552 0.014728862 0.043150128 0.003833878 1.81912965 0.409564825 1.593918091 0.296959046 0.11260578 Occallatibacter--other Bacteria Acidobacteriota 170 3 0.020919204 0.060797769 0.00827963 0 1.267406964 0.133703482 1.364623666 0.182311833 0.048608351 Sulfuriferula Bacteria Pseudomonadota 124 4 0.017086813 0.009778662 0.002974756 0.04556305 1.559673601 0.279836801 1.725701459 0.36285073 0.083013929 Telmatospirillum Bacteria Pseudomonadota 84 1 0.010203821 0.035713375 0 0 1 0 1 0 0 Candidatus Amoebophilus Bacteria Bacteroidota 72 3 0.010004746 0.001700637 0.02221065 0 1.152244499 0.076122249 1.117241379 0.05862069 0.01750156 Geothrix Bacteria Acidobacteriota 74 3 0.009363315 0.024234076 0 0.008537528 1.626796225 0.313398112 1.5477671 0.27388355 0.039514562 Sterolibacterium Bacteria Pseudomonadota 67 1 0.008138762 0.028485668 0 0 1 0 1 0 0 Victivallis Bacteria Lentisphaerota 60 3 0.007876118 0.014455414 0 0.013110999 1.995254261 0.49762713 1.965065502 0.482532751 0.015094379 Fodinisporobacter Bacteria Bacillota--other 57 1 0.006924022 0.024234076 0 0 1 0 1 0 0 Candidatus Megaera Bacteria Pseudomonadota 49 3 0.006502853 0 0.014214854 0.001437704 1.200233674 0.100116837 1.129882353 0.064941176 0.035175661 Miltoncostaea Bacteria Actinomycetota 49 3 0.006411763 0.004251592 0.012126386 0 1.624452558 0.312226279 1.481184454 0.240592227 0.071634052 Candidatus Protochlamydia Bacteria Chlamydiota 44 1 0.006277052 0 0.014646455 0 1 0 1 0 0 Acidisphaera Bacteria Pseudomonadota 43 1 0.006134392 0 0.014313581 0 1 0 1 0 0 Acidocella Bacteria Pseudomonadota 43 3 0.006123769 0.003826433 0.006657479 0.007620541 2.800595447 0.900297724 2.731166913 0.865583456 0.034714267 Terracidiphilus Bacteria Acidobacteriota 46 3 0.005845395 0.009353503 0.003569707 0.005750817 2.615995294 0.807997647 2.740932642 0.870466321 0.062468674 Blastococcus Bacteria Actinomycetota 45 1 0.005466333 0.019132165 0 0 1 0 1 0 0 Lutispora Bacteria Clostridiota 42 2 0.005138004 0.015305732 0.001784854 0 1.230097808 0.115048904 1.324324324 0.162162162 0.047113258 Aquella Bacteria Pseudomonadota 35 5 0.004785706 0.005379106 0.003746699 0.005750817 2.910323362 0.955161681 2.995110024 0.997555012 0.042393331 Ferrimicrobium Bacteria Actinomycetota 36 4 0.004663033 0.005101911 0.004603728 0.004313113 2.985494569 0.992747284 2.88 0.94 0.052747284 Sediminibacterium Bacteria Bacteroidota 29 1 0.004510116 0 0 0.015785406 1 0 1 0 0 Acidicaldus Bacteria Pseudomonadota 30 4 0.004205531 0.002125796 0.00621841 0.003265946 2.502981038 0.751490519 2.132701422 0.566350711 0.185139808 Ktedonosporobacter Bacteria Chloroflexota--other 29 1 0.004137148 0 0.009653345 0 1 0 1 0 0 Leclercia-Silvania-Enterobacter--other Bacteria Pseudomonadota 33 2 0.004044737 0.011479299 0.001784854 0 1.303628755 0.151814377 1.423529412 0.211764706 0.059950328 Candidatus Endonucleariibacter Bacteria Pseudomonadota 30 3 0.003825479 0.006377388 0.004674524 0 1.95362059 0.476810295 2 0.5 0.023189705 Spirochaeta--other Bacteria Spirochaetota 31 3 0.003814643 0.01105414 0.000892427 0.00095847 1.344049501 0.17202475 1.394775036 0.197387518 0.025362768 Fonticella Bacteria Clostridiota 30 2 0.003662268 0.011479299 0.000892427 0 1.154550476 0.077275238 1.219512195 0.109756098 0.032480859 Segatella Bacteria Bacteroidota 26 4 0.003313219 0.004251592 0.00393798 0.001437704 2.599792154 0.799896077 2.431654676 0.715827338 0.084068739 Gallionella Bacteria Pseudomonadota 22 1 0.003012333 0 0 0.010543165 1 0 1 0 0 Candidatus Finniella Bacteria Pseudomonadota 20 3 0.002853145 0.00226252 0.005148992 0 1.736597391 0.368298696 1.470588235 0.235294118 0.133004578 Ignavibacterium Bacteria Chlorobiota 20 2 0.002812872 0 0 0.009845053 1 0 1 0 0 Sideroxydans Bacteria Pseudomonadota 19 2 0.002614432 0.004251592 0 0.004898919 1.990040939 0.495020469 1.994475138 0.497237569 0.0022171 Rhodoblastus Bacteria Pseudomonadota 21 1 0.002550955 0.008928344 0 0 1 0 1 0 0 Truepera Bacteria Deinococcota 19 1 0.002308007 0.008078025 0 0 1 0 1 0 0 Xylanibacter Bacteria Bacteroidota 18 2 0.002292464 0.00552707 0.00166437 0 1.5521889 0.27609445 1.670103093 0.335051546 0.058957097 Deinococcus Bacteria Deinococcota 18 2 0.002210595 0.005952229 0.001189902 0 1.384453336 0.192226668 1.528301887 0.264150943 0.071924275 Nitrospira Bacteria Nitrospirota 15 2 0.002026394 0.003826433 0 0.003265946 1.987587095 0.493793548 1.923076923 0.461538462 0.032255086 Faucicola Bacteria Pseudomonadota 16 2 0.002015001 0.005101911 0.001300394 0 1.478670406 0.239335203 1.6 0.3 0.060664797 Rickettsia Bacteria Pseudomonadota 14 2 0.001875878 0 0.004377049 0 1 0 1 0 0 Bifidobacterium Bacteria Actinomycetota 14 2 0.001802778 0.004676751 0 0.001632973 1.622448492 0.311224246 1.507692308 0.253846154 0.057378092 Govania Bacteria Pseudomonadota 14 1 0.001700637 0.005952229 0 0 1 0 1 0 0 Alicyclobacillus Bacteria Bacillota--other 12 3 0.001616824 0 0.00185565 0.002875409 1.911205706 0.455602853 2 0.5 0.044397147 Candidatus Velamenicoccus Bacteria Candidatus Omnitrophota 11 3 0.001552801 0.001275478 0 0.004159326 1.560593169 0.280296584 1.657534247 0.328767123 0.048470539 Candidatus Lachnoclostridium--other Bacteria Clostridiota 11 1 0.001336215 0.004676751 0 0 1 0 1 0 0 Cellulomonas Bacteria Actinomycetota 11 1 0.001336215 0.004676751 0 0 1 0 1 0 0 Thermodesulfobium Bacteria Thermodesulfobiota 11 1 0.001336215 0.004676751 0 0 1 0 1 0 0 Candidatus Tyrphobacter Bacteria Vulcanimicrobiota 9 1 0.001253952 0 0.002925887 0 1 0 1 0 0 Aquicella Bacteria Pseudomonadota 7 2 0.001032858 0 0 0.003615002 1 0 1 0 0 Candidatus Bodonicaedibacter Bacteria Pseudomonadota 7 1 0.000998622 0 0.002330118 0 1 0 1 0 0 Thermogemmatispora Bacteria Chloroflexota--other 7 2 0.00090388 0.001700637 0.000975296 0 1.863109699 0.431554849 1.96 0.48 0.048445151 Candidatus Cytomitobacter Bacteria Pseudomonadota 6 1 0.000855962 0 0.001997244 0 1 0 1 0 0 Neochlamydia Bacteria Chlamydiota 6 2 0.000771217 0.001700637 0.000665748 0 1.678899203 0.339449602 1.8 0.4 0.060550398 Adhaeribacter Bacteria Bacteroidota 6 1 0.000728844 0.002550955 0 0 1 0 1 0 0 Anaerobacterium Bacteria Clostridiota 6 1 0.000728844 0.002550955 0 0 1 0 1 0 0 Hymenobacter Bacteria Bacteroidota 6 1 0.000728844 0.002550955 0 0 1 0 1 0 0 Microbacter Bacteria Bacteroidota 6 1 0.000728844 0.002550955 0 0 1 0 1 0 0 Telluria Bacteria Pseudomonadota 6 1 0.000728844 0.002550955 0 0 1 0 1 0 0 Candidatus Bathycorpusculum Archaea Thermoproteota 4 1 0.000622085 0 0 0.002177297 1 0 1 0 0 Flaviaestuariibacter Bacteria Bacteroidota 5 1 0.00060737 0.002125796 0 0 1 0 1 0 0 Geodermatophilus Bacteria Actinomycetota 5 1 0.00060737 0.002125796 0 0 1 0 1 0 0 Metaclostridioides Bacteria Clostridiota 5 1 0.00060737 0.002125796 0 0 1 0 1 0 0 Methylobacterium--other Bacteria Pseudomonadota 5 1 0.00060737 0.002125796 0 0 1 0 1 0 0 Pseudogemmatithrix Bacteria Gemmatimonadota 4 2 0.000559169 0 0.000665748 0.00095847 1.937082809 0.468541404 2 0.5 0.031458596 Duncaniella Bacteria Bacteroidota 4 1 0.000509958 0 0.001189902 0 1 0 1 0 0 Cerasibacillus Bacteria Bacillota 4 1 0.000485896 0.001700637 0 0 1 0 1 0 0 Fervidicella Bacteria Clostridiota 4 1 0.000485896 0.001700637 0 0 1 0 1 0 0 Syntrophobacter Bacteria Desulfobacterota 4 1 0.000485896 0.001700637 0 0 1 0 1 0 0 Porphyrobacter Bacteria Pseudomonadota 3 1 0.000466564 0 0 0.001632973 1 0 1 0 0 Sutterella Bacteria Pseudomonadota 3 1 0.000466564 0 0 0.001632973 1 0 1 0 0 Lactobacillus Bacteria Bacillota 3 1 0.000427981 0 0.000998622 0 1 0 1 0 0 Collinsella Bacteria Actinomycetota 3 1 0.000417984 0 0.000975296 0 1 0 1 0 0 Aurantibacillus Bacteria Bacteroidota 3 1 0.000410773 0 0 0.001437704 1 0 1 0 0 Candidatus Nitrosotenuis Archaea Thermoproteota 3 1 0.000410773 0 0 0.001437704 1 0 1 0 0 Lacunisphaera Bacteria Verrucomicrobiota 3 1 0.000382469 0 0.000892427 0 1 0 1 0 0 Daejeonella Bacteria Bacteroidota 3 1 0.000364422 0.001275478 0 0 1 0 1 0 0 Pseudosphingomonas Bacteria Pseudomonadota 3 1 0.000364422 0.001275478 0 0 1 0 1 0 0 Roseisolibacter Bacteria Gemmatimonadota 3 1 0.000364422 0.001275478 0 0 1 0 1 0 0 Ruminiclostridium Bacteria Clostridiota 3 1 0.000364422 0.001275478 0 0 1 0 1 0 0 Caulobacter Bacteria Pseudomonadota 2 1 0.000311042 0 0 0.001088649 1 0 1 0 0 Desulfomonile Bacteria Desulfobacterota 2 1 0.000273848 0 0 0.00095847 1 0 1 0 0 Fimbriimonas Bacteria Armatimonadota 2 1 0.000273848 0 0 0.00095847 1 0 1 0 0 Ellin517 Bacteria Verrucomicrobiota 2 1 0.000254979 0 0.000594951 0 1 0 1 0 0 Gaiella Bacteria Actinomycetota 2 1 0.000254979 0 0.000594951 0 1 0 1 0 0 Peredibacter Bacteria Bdellovibrionota 2 1 0.000254979 0 0.000594951 0 1 0 1 0 0 Alistipes Bacteria Bacteroidota 2 1 0.000242948 0.000850318 0 0 1 0 1 0 0 Candidatus Planktophila Bacteria Actinomycetota 2 1 0.000242948 0.000850318 0 0 1 0 1 0 0 Nocardioides Bacteria Actinomycetota 2 1 0.000242948 0.000850318 0 0 1 0 1 0 0 PMMR1 Bacteria Pseudomonadota 2 1 0.000242948 0.000850318 0 0 1 0 1 0 0 Thermincola--other Bacteria Desulfotomaculota 2 1 0.000242948 0.000850318 0 0 1 0 1 0 0 Veillonella Bacteria Desulfotomaculota 2 1 0.000242948 0.000850318 0 0 1 0 1 0 0