LOCUS pl_paba 5315 bp ds-DNA circular 22-NOV-2023 DEFINITION . COMMENT Imported using the Genbank importer. File name: Supplementary Data 14_pl_paba.gb Imported using the Genbank importer. File name: ptet_strongrbs_gfp.gb FEATURES Location/Qualifiers misc_feature 1..1869 /label="pabAB" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" CDS 1..1869 /label="pabAB translation" /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /translation="MRVLIIDNYDSFTFNLATYVEEVTGAAPTVVPNDAQIDETLFDAVIISPGPGHPGVAADFGSCRGVIERGLVPVLGVCLGHQGIALAHGGAVGPAPVPVHGQVTRIHHDGSELFDAIPPQFDAVRYHSLVATDLPPELEVTARTGDGLIMALRHRELPQWGVQFHPESIGGQFGHRIMANFLSLARRQAHRWEITEHVVETSVDPAAVFETLFAGSEHAFWLDDPQGTTYMGDASGPHARIRTHRVGEGELFDWLRDDLRRNRVAPGVGFRLGWVGYLGYEMKAECGVDNRHASSHPDAHLIFADRAIAIEPGRVWLMALGEQGEWFAEMTAALGQLRPPRAAAAPAAQLTVRDDRDSYLDMIARAQELITRGESYEICLTTQLRAEVEVDPLAAYLALRAANPTSYGSFLQLGEMAVLSSSPERFITIDASGRVESKPIKGTRPRGSTEQEDAALIADLTDNPKDRAENLMIVDLVRNDLARGAQPATVQVEKLFDVETYATVHQLVSTITAQLEGKDPIDCVRAAFPGGSMTGAPKIRTMEIIDELETGPRGVYSGGLGYFSLDGAVDLSMVIRTVVYTPGVLEYGVGGAILALSDPAAEWEEIRVKSRPLLGLLGVEFP*" RBS 1887..1898 /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" CDS 1905..2714 /label="pabC translation" /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQRLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATRILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNADEALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVEVQASLEESLQADEMVICNALMPVMPVCACGDVSFSSATLYEYLAPLCERPN*" CDS 1905..2714 /label="pabC" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" /note="locus_tag: BW25113_1096 gene_synonym: JW1082 EC_number: 4.1.3.38 function: enzyme; Biosynthesis of cofactors, carriers:Folic acid experiment: N-terminus verified by Edman degradation:PMID1644759,2071583 note: 4-amino-4-deoxychorismate lyase transl_table: 11 product: 4-amino-4-deoxychorismate lyase component ofpara-aminobenzoate synthase multienzyme complex protein_id: AIN31559.1 translation: MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQRLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATRILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNADEALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVEVQASLEESLQADEMVICNALMPVMPVCACGDVSFSSATLYEYLAPLCERPN" /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQRLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATRILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNADEALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVEVQASLEESLQADEMVICNALMPVMPVCACGDVSFSSATLYEYLAPLCERPN*" terminator 2741..2812 /label="rrnB T1 terminator" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" terminator 2828..2855 /label="T7Te terminator" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" rep_origin complement(3017..3562) /label="p15A ori" /ApEinfo_revcolor="#c6c9d1" /ApEinfo_fwdcolor="#c6c9d1" terminator complement(3676..3770) /label="lambda t0 terminator" /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" CDS complement(3791..4450) /label="cat" /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*" CDS complement(3791..4450) /label="cat translation" /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*" promoter complement(4451..4553) /label="cat promoter" /ApEinfo_revcolor="#c7b0e3" /ApEinfo_fwdcolor="#c7b0e3" CDS complement(4583..5206) /label="tetR translation" /ApEinfo_revcolor="#84b0dc" /ApEinfo_fwdcolor="#84b0dc" /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*" CDS complement(4583..5206) /label="tetR" /ApEinfo_revcolor="#85dae9" /ApEinfo_fwdcolor="#85dae9" /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*" promoter 5225..5280 /label="tetR/tetA promoters" /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" misc_feature 5253..5283 /label="tet response element" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" RBS 5298..5309 /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" /ApEinfo_revcolor="#75c6a9" /ApEinfo_fwdcolor="#75c6a9" ORIGIN 1 ATGCGTGTGC TCATCATCGA TAACTACGAC TCTTTCACGT TTAATTTAGC GACATACGTA 61 GAAGAAGTTA CAGGCGCCGC CCCAACAGTA GTACCAAACG ACGCCCAAAT AGATGAAACC 121 TTATTTGATG CGGTTATAAT TTCTCCGGGT CCGGGACATC CTGGTGTCGC CGCCGACTTT 181 GGTTCCTGTC GTGGAGTTAT TGAGCGGGGC TTGGTGCCAG TACTGGGTGT TTGTTTGGGA 241 CACCAAGGTA TTGCCCTTGC GCATGGTGGT GCCGTTGGAC CCGCACCCGT GCCAGTTCAT 301 GGCCAAGTAA CTCGTATTCA TCATGATGGA TCAGAATTAT TTGATGCAAT TCCACCGCAA 361 TTTGATGCAG TACGGTATCA TAGTTTAGTT GCAACTGACC TCCCTCCTGA GCTTGAAGTG 421 ACAGCACGGA CGGGTGATGG TTTGATTATG GCTCTTCGTC ATCGTGAATT ACCACAATGG 481 GGAGTCCAAT TTCATCCCGA GTCTATTGGC GGACAATTTG GCCATCGCAT TATGGCTAAC 541 TTTCTTTCGC TTGCCCGCAG ACAGGCACAT CGTTGGGAAA TAACAGAACA CGTTGTCGAA 601 ACCTCTGTGG ATCCTGCAGC AGTCTTTGAA ACACTGTTTG CAGGCTCAGA ACATGCCTTT 661 TGGTTGGACG ACCCACAAGG GACGACTTAC ATGGGCGACG CGTCAGGCCC CCACGCTCGC 721 ATTCGGACGC ATCGTGTCGG TGAAGGTGAA TTGTTTGATT GGCTTCGCGA CGACTTGCGC 781 CGTAATCGGG TCGCACCAGG AGTTGGCTTT CGTCTTGGTT GGGTTGGTTA TTTGGGATAC 841 GAAATGAAAG CGGAGTGTGG AGTTGATAAC CGCCATGCAA GCAGTCACCC AGACGCTCAT 901 TTAATTTTCG CAGATCGAGC AATTGCGATA GAGCCTGGTC GTGTATGGCT TATGGCACTG 961 GGAGAACAAG GTGAATGGTT TGCTGAAATG ACTGCGGCTT TAGGTCAATT ACGGCCGCCT 1021 CGAGCAGCAG CAGCACCCGC AGCACAATTG ACTGTTCGTG ACGATCGAGA TAGTTATTTG 1081 GATATGATTG CTAGAGCACA AGAACTGATT ACTAGAGGTG AATCATACGA AATATGTCTT 1141 ACAACTCAAC TTCGTGCAGA AGTAGAAGTT GACCCTCTGG CAGCTTACTT AGCTTTAAGA 1201 GCAGCTAATC CGACGTCTTA CGGTAGCTTT CTCCAATTAG GCGAAATGGC CGTCTTATCC 1261 TCATCTCCCG AGCGTTTTAT AACTATTGAT GCTAGCGGTC GCGTCGAGTC AAAGCCAATT 1321 AAAGGTACTC GGCCCAGAGG ATCTACAGAA CAAGAAGACG CAGCTCTGAT AGCTGACCTG 1381 ACTGATAATC CTAAAGATCG TGCGGAAAAT TTAATGATAG TTGATTTGGT ACGTAACGAC 1441 CTTGCAAGAG GTGCCCAGCC CGCCACCGTC CAAGTCGAGA AATTGTTTGA TGTGGAAACG 1501 TATGCGACGG TGCATCAATT GGTATCTACT ATCACAGCGC AACTTGAAGG CAAAGATCCG 1561 ATAGATTGTG TCCGAGCCGC CTTTCCAGGC GGGAGTATGA CTGGGGCCCC GAAAATACGT 1621 ACTATGGAAA TTATTGATGA ATTGGAAACG GGACCTCGAG GAGTTTACTC TGGCGGTCTT 1681 GGTTACTTTA GCTTGGACGG CGCAGTAGAC TTGTCCATGG TCATTAGAAC TGTCGTGTAT 1741 ACGCCAGGTG TTCTCGAATA TGGCGTTGGC GGAGCAATAC TTGCACTCAG CGATCCAGCA 1801 GCCGAATGGG AAGAAATACG AGTCAAGTCA CGCCCGTTGC TCGGCTTGCT GGGTGTTGAA 1861 TTTCCATAAT AAAATTCAAA AGATCTAAAG AGGAGAAAGG ATCTatgttc ttaattaacg 1921 gtcataagca ggaatcgctg gcagtaagcg atcgggcaac gcagtttggt gatggttgtt 1981 ttaccaccgc cagagttatc gacggtaaag tcagtttgtt atcggcgcat atccagcgac 2041 tacaggatgc ttgtcagcgg ttgatgattt cctgtgactt ctggcctcag cttgaacaag 2101 agatgaaaac gctggcagca gaacagcaaa atggtgtgct gaaagtcgtg atcagtcgcg 2161 gtagtggcgg gcgagggtac agcacattga acagcggacc ggcaacgcgg attctctccg 2221 ttacggctta tcctgcacat tacgaccgtt tgcgtaacga ggggattacg ttggcgctaa 2281 gcccggtgcg gctggggcgc aatcctcatc ttgcaggtat taaacatctc aatcgtcttg 2341 agcaagtatt gattcgctct catcttgagc agacaaacgc tgatgaggcg ctggtccttg 2401 acagcgaagg gtgggttacg gaatgctgtg cggctaattt gttctggcgg aagggcaacg 2461 tagtttatac gccgcgactg gatcaggcag gtgttaacgg cattatgcga caattctgta 2521 tccgtttgct ggcacaatcc tcttatcagc ttgtcgaagt gcaagcctct ctggaagagt 2581 cgttgcaggc agatgagatg gttatttgta atgcgttaat gccagtgatg cccgtatgtg 2641 cctgtggcga tgtctccttt tcgtcagcaa cgttatatga atatttagcc ccactttgtg 2701 agcgcccgaa ttagTGACTC GAGTAAGGAT CTCCAGGCAT CAAATAAAAC GAAAGGCTCA 2761 GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCTACTAGAG 2821 TCACACTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTTAT ACCTAGGGAT ATATTCCGCT 2881 TCCTCGCTCA CTGACTCGCT ACGCTCGGTC GTTCGACTGC GGCGAGCGGA AATGGCTTAC 2941 GAACGGGGCG GAGATTTCCT GGAAGATGCC AGGAAGATAC TTAACAGGGA AGTGAGAGGG 3001 CCGCGGCAAA GCCGTTTTTC CATAGGCTCC GCCCCCCTGA CAAGCATCAC GAAATCTGAC 3061 GCTCAAATCA GTGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 3121 GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC ATTCCGCTGT 3181 TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT CCGGGTAGGC AGTTCGCTCC 3241 AAGCTGGACT GTATGCACGA ACCCCCCGTT CAGTCCGACC GCTGCGCCTT ATCCGGTAAC 3301 TATCGTCTTG AGTCCAACCC GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT 3361 AATTGATTTA GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC 3421 AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT TGGTAGCTCA 3481 GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG TTTTCAGAGC AAGAGATTAC 3541 GCGCAGACCA AAACGATCTC AAGAAGATCA TCTTATTAAT CAGATAAAAT ATTTCTAGAT 3601 TTCAGTGCAA TTTATCTCTT CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT 3661 GTTACTAGTG CTTGGATTCT CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA 3721 CAAATCCAGA TGGAGTTCTG AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCGAGCT 3781 CGATATCAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA 3841 TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA 3901 GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT 3961 CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA 4021 AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA 4081 CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG 4141 ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA 4201 TCACCAGCTC ACCGTCTTTC ATTGCCATAC GAAATTCCGG ATGAGCATTC ATCAGGCGGG 4261 CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA 4321 AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG 4381 CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT 4441 TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG 4501 GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC 4561 ATTTTCGCCA GATATCGACG TCTTAAGACC CACTTTCACA TTTAAGTTGT TTTTCTAATC 4621 CGCATATGAT CAATTCAAGG CCGAATAAGA AGGCTGGCTC TGCACCTTGG TGATCAAATA 4681 ATTCGATAGC TTGTCGTAAT AATGGCGGCA TACTATCAGT AGTAGGTGTT TCCCTTTCTT 4741 CTTTAGCGAC TTGATGCTCT TGATCTTCCA ATACGCAACC TAAAGTAAAA TGCCCCACAG 4801 CGCTGAGTGC ATATAATGCA TTCTCTAGTG AAAAACCTTG TTGGCATAAA AAGGCTAATT 4861 GATTTTCGAG AGTTTCATAC TGTTTTTCTG TAGGCCGTGT ACCTAAATGT ACTTTTGCTC 4921 CATCGCGATG ACTTAGTAAA GCACATCTAA AACTTTTAGC GTTATTACGT AAAAAATCTT 4981 GCCAGCTTTC CCCTTCTAAA GGGCAAAAGT GAGTATGGTG CCTATCTAAC ATCTCAATGG 5041 CTAAGGCGTC GAGCAAAGCC CGCTTATTTT TTACATGCCA ATACAATGTA GGCTGCTCTA 5101 CACCTAGCTT CTGGGCGAGT TTACGGGTTG TTAAACCTTC GATTCCGACC TCATTAAGCA 5161 GCTCTAATGC GCTGTTAATC ACTTTACTTT TATCTAATCT AGACATCATT AATTCCTAAT 5221 TTTTGTTGAC ACTCTATCGT TGATAGAGTT ATTTTACCAC TCCCTATCAG TGATAGAGAA 5281 AAGAATTCAA AAGATCTAAA GAGGAGAAAG GATCT //