LOCUS       pl_paba                 5315 bp ds-DNA     circular     22-NOV-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: Supplementary Data
            14_pl_paba.gb Imported using the Genbank importer. File name:
            ptet_strongrbs_gfp.gb
FEATURES             Location/Qualifiers
     misc_feature    1..1869
                     /label="pabAB"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1..1869
                     /label="pabAB translation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MRVLIIDNYDSFTFNLATYVEEVTGAAPTVVPNDAQIDETLFDAVIISPGPGHPGVAADFGSCRGVIERGLVPVLGVCLGHQGIALAHGGAVGPAPVPVHGQVTRIHHDGSELFDAIPPQFDAVRYHSLVATDLPPELEVTARTGDGLIMALRHRELPQWGVQFHPESIGGQFGHRIMANFLSLARRQAHRWEITEHVVETSVDPAAVFETLFAGSEHAFWLDDPQGTTYMGDASGPHARIRTHRVGEGELFDWLRDDLRRNRVAPGVGFRLGWVGYLGYEMKAECGVDNRHASSHPDAHLIFADRAIAIEPGRVWLMALGEQGEWFAEMTAALGQLRPPRAAAAPAAQLTVRDDRDSYLDMIARAQELITRGESYEICLTTQLRAEVEVDPLAAYLALRAANPTSYGSFLQLGEMAVLSSSPERFITIDASGRVESKPIKGTRPRGSTEQEDAALIADLTDNPKDRAENLMIVDLVRNDLARGAQPATVQVEKLFDVETYATVHQLVSTITAQLEGKDPIDCVRAAFPGGSMTGAPKIRTMEIIDELETGPRGVYSGGLGYFSLDGAVDLSMVIRTVVYTPGVLEYGVGGAILALSDPAAEWEEIRVKSRPLLGLLGVEFP*"
     RBS             1887..1898
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1905..2714
                     /label="pabC translation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQRLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATRILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNADEALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVEVQASLEESLQADEMVICNALMPVMPVCACGDVSFSSATLYEYLAPLCERPN*"
     CDS             1905..2714
                     /label="pabC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="locus_tag: BW25113_1096 gene_synonym: JW1082 EC_number: 4.1.3.38 function: enzyme; Biosynthesis of cofactors, carriers:Folic acid experiment: N-terminus verified by Edman degradation:PMID1644759,2071583 note: 4-amino-4-deoxychorismate lyase transl_table: 11 product: 4-amino-4-deoxychorismate lyase component ofpara-aminobenzoate synthase multienzyme complex protein_id: AIN31559.1 translation: MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQRLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATRILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNADEALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVEVQASLEESLQADEMVICNALMPVMPVCACGDVSFSSATLYEYLAPLCERPN"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQRLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATRILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNADEALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVEVQASLEESLQADEMVICNALMPVMPVCACGDVSFSSATLYEYLAPLCERPN*"
     terminator      2741..2812
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     terminator      2828..2855
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      complement(3017..3562)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     terminator      complement(3676..3770)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(3791..4450)
                     /label="cat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     CDS             complement(3791..4450)
                     /label="cat translation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     promoter        complement(4451..4553)
                     /label="cat promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(4583..5206)
                     /label="tetR translation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*"
     CDS             complement(4583..5206)
                     /label="tetR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*"
     promoter        5225..5280
                     /label="tetR/tetA promoters"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5253..5283
                     /label="tet response element"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     RBS             5298..5309
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 ATGCGTGTGC TCATCATCGA TAACTACGAC TCTTTCACGT TTAATTTAGC GACATACGTA
       61 GAAGAAGTTA CAGGCGCCGC CCCAACAGTA GTACCAAACG ACGCCCAAAT AGATGAAACC
      121 TTATTTGATG CGGTTATAAT TTCTCCGGGT CCGGGACATC CTGGTGTCGC CGCCGACTTT
      181 GGTTCCTGTC GTGGAGTTAT TGAGCGGGGC TTGGTGCCAG TACTGGGTGT TTGTTTGGGA
      241 CACCAAGGTA TTGCCCTTGC GCATGGTGGT GCCGTTGGAC CCGCACCCGT GCCAGTTCAT
      301 GGCCAAGTAA CTCGTATTCA TCATGATGGA TCAGAATTAT TTGATGCAAT TCCACCGCAA
      361 TTTGATGCAG TACGGTATCA TAGTTTAGTT GCAACTGACC TCCCTCCTGA GCTTGAAGTG
      421 ACAGCACGGA CGGGTGATGG TTTGATTATG GCTCTTCGTC ATCGTGAATT ACCACAATGG
      481 GGAGTCCAAT TTCATCCCGA GTCTATTGGC GGACAATTTG GCCATCGCAT TATGGCTAAC
      541 TTTCTTTCGC TTGCCCGCAG ACAGGCACAT CGTTGGGAAA TAACAGAACA CGTTGTCGAA
      601 ACCTCTGTGG ATCCTGCAGC AGTCTTTGAA ACACTGTTTG CAGGCTCAGA ACATGCCTTT
      661 TGGTTGGACG ACCCACAAGG GACGACTTAC ATGGGCGACG CGTCAGGCCC CCACGCTCGC
      721 ATTCGGACGC ATCGTGTCGG TGAAGGTGAA TTGTTTGATT GGCTTCGCGA CGACTTGCGC
      781 CGTAATCGGG TCGCACCAGG AGTTGGCTTT CGTCTTGGTT GGGTTGGTTA TTTGGGATAC
      841 GAAATGAAAG CGGAGTGTGG AGTTGATAAC CGCCATGCAA GCAGTCACCC AGACGCTCAT
      901 TTAATTTTCG CAGATCGAGC AATTGCGATA GAGCCTGGTC GTGTATGGCT TATGGCACTG
      961 GGAGAACAAG GTGAATGGTT TGCTGAAATG ACTGCGGCTT TAGGTCAATT ACGGCCGCCT
     1021 CGAGCAGCAG CAGCACCCGC AGCACAATTG ACTGTTCGTG ACGATCGAGA TAGTTATTTG
     1081 GATATGATTG CTAGAGCACA AGAACTGATT ACTAGAGGTG AATCATACGA AATATGTCTT
     1141 ACAACTCAAC TTCGTGCAGA AGTAGAAGTT GACCCTCTGG CAGCTTACTT AGCTTTAAGA
     1201 GCAGCTAATC CGACGTCTTA CGGTAGCTTT CTCCAATTAG GCGAAATGGC CGTCTTATCC
     1261 TCATCTCCCG AGCGTTTTAT AACTATTGAT GCTAGCGGTC GCGTCGAGTC AAAGCCAATT
     1321 AAAGGTACTC GGCCCAGAGG ATCTACAGAA CAAGAAGACG CAGCTCTGAT AGCTGACCTG
     1381 ACTGATAATC CTAAAGATCG TGCGGAAAAT TTAATGATAG TTGATTTGGT ACGTAACGAC
     1441 CTTGCAAGAG GTGCCCAGCC CGCCACCGTC CAAGTCGAGA AATTGTTTGA TGTGGAAACG
     1501 TATGCGACGG TGCATCAATT GGTATCTACT ATCACAGCGC AACTTGAAGG CAAAGATCCG
     1561 ATAGATTGTG TCCGAGCCGC CTTTCCAGGC GGGAGTATGA CTGGGGCCCC GAAAATACGT
     1621 ACTATGGAAA TTATTGATGA ATTGGAAACG GGACCTCGAG GAGTTTACTC TGGCGGTCTT
     1681 GGTTACTTTA GCTTGGACGG CGCAGTAGAC TTGTCCATGG TCATTAGAAC TGTCGTGTAT
     1741 ACGCCAGGTG TTCTCGAATA TGGCGTTGGC GGAGCAATAC TTGCACTCAG CGATCCAGCA
     1801 GCCGAATGGG AAGAAATACG AGTCAAGTCA CGCCCGTTGC TCGGCTTGCT GGGTGTTGAA
     1861 TTTCCATAAT AAAATTCAAA AGATCTAAAG AGGAGAAAGG ATCTatgttc ttaattaacg
     1921 gtcataagca ggaatcgctg gcagtaagcg atcgggcaac gcagtttggt gatggttgtt
     1981 ttaccaccgc cagagttatc gacggtaaag tcagtttgtt atcggcgcat atccagcgac
     2041 tacaggatgc ttgtcagcgg ttgatgattt cctgtgactt ctggcctcag cttgaacaag
     2101 agatgaaaac gctggcagca gaacagcaaa atggtgtgct gaaagtcgtg atcagtcgcg
     2161 gtagtggcgg gcgagggtac agcacattga acagcggacc ggcaacgcgg attctctccg
     2221 ttacggctta tcctgcacat tacgaccgtt tgcgtaacga ggggattacg ttggcgctaa
     2281 gcccggtgcg gctggggcgc aatcctcatc ttgcaggtat taaacatctc aatcgtcttg
     2341 agcaagtatt gattcgctct catcttgagc agacaaacgc tgatgaggcg ctggtccttg
     2401 acagcgaagg gtgggttacg gaatgctgtg cggctaattt gttctggcgg aagggcaacg
     2461 tagtttatac gccgcgactg gatcaggcag gtgttaacgg cattatgcga caattctgta
     2521 tccgtttgct ggcacaatcc tcttatcagc ttgtcgaagt gcaagcctct ctggaagagt
     2581 cgttgcaggc agatgagatg gttatttgta atgcgttaat gccagtgatg cccgtatgtg
     2641 cctgtggcga tgtctccttt tcgtcagcaa cgttatatga atatttagcc ccactttgtg
     2701 agcgcccgaa ttagTGACTC GAGTAAGGAT CTCCAGGCAT CAAATAAAAC GAAAGGCTCA
     2761 GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCTACTAGAG
     2821 TCACACTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTTAT ACCTAGGGAT ATATTCCGCT
     2881 TCCTCGCTCA CTGACTCGCT ACGCTCGGTC GTTCGACTGC GGCGAGCGGA AATGGCTTAC
     2941 GAACGGGGCG GAGATTTCCT GGAAGATGCC AGGAAGATAC TTAACAGGGA AGTGAGAGGG
     3001 CCGCGGCAAA GCCGTTTTTC CATAGGCTCC GCCCCCCTGA CAAGCATCAC GAAATCTGAC
     3061 GCTCAAATCA GTGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     3121 GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC ATTCCGCTGT
     3181 TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT CCGGGTAGGC AGTTCGCTCC
     3241 AAGCTGGACT GTATGCACGA ACCCCCCGTT CAGTCCGACC GCTGCGCCTT ATCCGGTAAC
     3301 TATCGTCTTG AGTCCAACCC GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT
     3361 AATTGATTTA GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC
     3421 AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT TGGTAGCTCA
     3481 GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG TTTTCAGAGC AAGAGATTAC
     3541 GCGCAGACCA AAACGATCTC AAGAAGATCA TCTTATTAAT CAGATAAAAT ATTTCTAGAT
     3601 TTCAGTGCAA TTTATCTCTT CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT
     3661 GTTACTAGTG CTTGGATTCT CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA
     3721 CAAATCCAGA TGGAGTTCTG AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCGAGCT
     3781 CGATATCAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA
     3841 TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA
     3901 GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT
     3961 CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA
     4021 AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA
     4081 CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG
     4141 ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA
     4201 TCACCAGCTC ACCGTCTTTC ATTGCCATAC GAAATTCCGG ATGAGCATTC ATCAGGCGGG
     4261 CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA
     4321 AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG
     4381 CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT
     4441 TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG
     4501 GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA TCAACGTCTC
     4561 ATTTTCGCCA GATATCGACG TCTTAAGACC CACTTTCACA TTTAAGTTGT TTTTCTAATC
     4621 CGCATATGAT CAATTCAAGG CCGAATAAGA AGGCTGGCTC TGCACCTTGG TGATCAAATA
     4681 ATTCGATAGC TTGTCGTAAT AATGGCGGCA TACTATCAGT AGTAGGTGTT TCCCTTTCTT
     4741 CTTTAGCGAC TTGATGCTCT TGATCTTCCA ATACGCAACC TAAAGTAAAA TGCCCCACAG
     4801 CGCTGAGTGC ATATAATGCA TTCTCTAGTG AAAAACCTTG TTGGCATAAA AAGGCTAATT
     4861 GATTTTCGAG AGTTTCATAC TGTTTTTCTG TAGGCCGTGT ACCTAAATGT ACTTTTGCTC
     4921 CATCGCGATG ACTTAGTAAA GCACATCTAA AACTTTTAGC GTTATTACGT AAAAAATCTT
     4981 GCCAGCTTTC CCCTTCTAAA GGGCAAAAGT GAGTATGGTG CCTATCTAAC ATCTCAATGG
     5041 CTAAGGCGTC GAGCAAAGCC CGCTTATTTT TTACATGCCA ATACAATGTA GGCTGCTCTA
     5101 CACCTAGCTT CTGGGCGAGT TTACGGGTTG TTAAACCTTC GATTCCGACC TCATTAAGCA
     5161 GCTCTAATGC GCTGTTAATC ACTTTACTTT TATCTAATCT AGACATCATT AATTCCTAAT
     5221 TTTTGTTGAC ACTCTATCGT TGATAGAGTT ATTTTACCAC TCCCTATCAG TGATAGAGAA
     5281 AAGAATTCAA AAGATCTAAA GAGGAGAAAG GATCT
//