Mammalian cell display with automated oligo design and library assembly allows for rapid residue level conformational epitope mapping
Supplementary data 2
Niklas Berndt
Thalén1†, Maximilian Karlander1†, Magnus
Lundqvist1, Helena Persson2,
Camilla Hofström2, S. Pauliina Turunen2, Magdalena
Godzwon3, Anna-Luisa Volk1,
Magdalena Malm1, Mats Ohlin3, Johan
Rockberg1#
1KTH - Royal Institute of Technology, Dept. Protein science;
Stockholm; SE-106 91; Sweden
2Science for Life Laboratory, Drug Discovery and Development
Platform & School of
Biotechnology, KTH-Royal Institute of Technology, Stockholm,
Sweden
3Department of Immunotechnology, Lund University, Lund,
Sweden
†Joint authors
# To whom correspondence should be addressed: Prof. Johan
Rockberg
KTH Royal Institute of Technology, Dept Protein science,
Roslagstullsbacken 21, 10691,
Stockholm Sweden
Phone: +46 8 790 99 88
Camilla Hofström2, S. Pauliina Turunen2, Magdalena Godzwon3, Anna-Luisa Volk1,
Magdalena Malm1, Mats Ohlin3, Johan Rockberg1#
1KTH - Royal Institute of Technology, Dept. Protein science; Stockholm; SE-106 91; Sweden
2Science for Life Laboratory, Drug Discovery and Development Platform & School of
Biotechnology, KTH-Royal Institute of Technology, Stockholm, Sweden
3Department of Immunotechnology, Lund University, Lund, Sweden
†Joint authors
# To whom correspondence should be addressed: Prof. Johan Rockberg
KTH Royal Institute of Technology, Dept Protein science, Roslagstullsbacken 21, 10691,
Stockholm Sweden
Phone: +46 8 790 99 88
1 Description of data
This document contains all flow cytometry plots generated for this article. They are organized based on the antibody/ligand and the title of each plot refers the position tested.
- Expression - Alexa 488 refers to the fluorophore used to determine expression. A rabbit anti-HA antibody was used as primary antibody and an Alexa488 conjugated anti-rabbit antibody was sused as secondary antibody.
- Binding - Alexa 647 refers to the fluorophore used to determine binding. As all antibodies used are human and ACE2 was human FC-coupled an Alexa647 conjugated anti-human antibody was used for detection.
2 ACE2
3 CR3022
4 MO176_156
5 MO176_301
6 MO176_317
