LOCUS Addgene100845 5917 bp DNA circular SYN 06-MAY-2026 DEFINITION pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Addgene plasmid #100845. ACCESSION ADDGENE_100845 VERSION ADDGENE_100845 KEYWORDS AAV; GCaMP6s; FLEX; Synapsin; WPRE; SV40; Ampicillin. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct other sequences; artificial sequences. REFERENCE 1 (bases 1 to 5917) AUTHORS Chen,T.W. et al. TITLE Ultrasensitive fluorescent proteins for imaging neuronal activity JOURNAL Nature 499(7458), 295-300 (2013) PUBMED 23868258 COMMENT GBK generated from publicly visible Addgene metadata/map and user-provided COMMENT Addgene_NGS_Result sequence. Feature coordinates remain approximate/inferred COMMENT from the original template and should be verified before cloning or publication. FEATURES Location/Qualifiers source 1..5917 /organism="synthetic DNA construct" /mol_type="other DNA" /plasmid="pAAV.Syn.Flex.GCaMP6s.WPRE.SV40" misc_feature ~120..~280 /label="AAV2 ITR" promoter ~350..~690 /label="hSyn promoter" misc_feature ~735..~830 /label="lox2272" misc_feature ~880..~970 /label="loxP" CDS complement(1070..2090) /label="GCaMP6s" /note="Cre-dependent/FLEX orientation inferred from map" misc_feature ~2260..~2450 /label="lox2272" misc_feature ~2460..~2530 /label="loxP" misc_feature ~2700..~3150 /label="WPRE" polyA_signal ~3150..~3400 /label="SV40 poly(A) signal" rep_origin ~3650..~4300 /label="ori" CDS complement(~4680..~5540) /label="AmpR" promoter complement(~5480..~5700) /label="AmpR promoter" rep_origin ~520..~950 /label="f1 ori" ORIGIN 1 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 61 ccagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac 121 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat 181 cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg 241 ctctaggaag atctctgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg 301 atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca 361 acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg 421 cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc 481 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg 541 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg 601 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc 661 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg 721 cagtcgaatt caagctgcta gcaaggatcc ccataacttc gtataaagta tcctatacga 781 agttatatca aaataggaag accaatgctt caccatcgac ccgaattgcc aagcatcacc 841 atcgacccat aacttcgtat aatgtatgct atacgaagtt atactagagc tttcacttcg 901 ctgtcatcat ttgtacaaac tcttcgtagt ttacctgacc atccccatcg atgtctgctt 961 ccctgatcat ttcatcaacc tcttcatctg ttaacttctc tccaaggttt gtcatcacgt 1021 ggcgaagctc tgctgcactg atgtagccat tgccatcctt atcaaacaca ccgaacgctt 1081 ctctaatttc ttcttccgtg tccctgtatt tcatttttct tgccatcatt gtcaggaact 1141 cagggaagtc gattgtgccg tcaccgtcgg catctacttc attgatcatg tcctgcagct 1201 ctgcttctgt ggggttctgc cccagagacc gcatcaccgt ccccagctcc ttggttgtta 1261 ttgtcccatc cccgtccttg tcaaataggg agaaagcctc tttaaattct gcgatctgct 1321 cttcagtcag ttggtccggc aggttgtact ccagcttgtg ccccaggatg ttgccgtcct 1381 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca 1441 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggatgt 1501 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc 1561 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct 1621 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcaccct 1681 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca 1741 ccaccccggt gaacagctcc tcgcccttgc tcaccatgct ccctccggta ccgcccttgt 1801 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt 1861 gatcgcgctt ctcgttgggg tctttcgaaa gtttggactg cacgctcagg tagtggttgt 1921 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg taggcgagct 1981 gcacgccgcc gtcctcgatg ttgtggcgga tgtggaagtt cgccttgatg ccgttcttct 2041 gcttgtcggc cttgatatag acgttctcga gtgagctcag ccgacctata gctctgactg 2101 cgtgacctgt cttattccac ttacgacgtg atgagtcgac catggtggcg agatccttat 2161 cgtcatcgtc gtacagatcc cgacccattt gctgtccacc agtcatgcta gccataccat 2221 gatgatgatg atgatgagaa cccatggtgg cggatcctag tataacttcg tataggatac 2281 tttatacgaa gttatcattg ggattcttcc tattttgatc caagcatcac catcgaccct 2341 ctagtccaga tctcaccatc gacccataac ttcgtatagc atacattata cgaagttatg 2401 tccctcgaag aggttcgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa 2461 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 2521 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 2581 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 2641 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 2701 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 2761 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 2821 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 2881 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 2941 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 3001 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgac ccgggcggcc 3061 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 3121 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 3181 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 3241 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa 3301 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca 3361 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 3421 ccgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcattaatga 3481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 3541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 3601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 3661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 3721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 3781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 3841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 3901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 3961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 4021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 4081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 4141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 4201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 4261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 4321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 4381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 4441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 4501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 4561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 4621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 4681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 4741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 4801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 4861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 4921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 4981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 5041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 5101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 5161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 5221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 5281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 5341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 5401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg 5461 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 5521 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 5581 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 5641 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 5701 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 5761 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 5821 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 5881 ccgcgcttaa tgcgccgcta cagggcgcgt cccattc //