Movie EV1 (associated to Figure 1E). Live cell imaging of an EGFP-OCLN transmigrating primary monocyte. 3D time lapse spinning disk confocal imaging of a primary monocyte transduced with EGFP-OCLN (red) on hCMEC/D3 monolayer pre-stained with CellTrace (cyan) before the beginning of imaging. Z stacks images were taken every 10 min for 9 h and 30 min. Scale bar: 10 μm. 