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["The double-domain cytidine deaminase APOBEC3G is a cellular site-specific RNA editing enzyme "]
[]
["Shraddha Sharma1, Santosh K. Patnaik2, Robert T. Taggart1, Bora E. Baysal1*"]
["Departments of 1Pathology and 2Thoracic Surgery, Roswell Park Cancer Institute"]
["Buffalo, NY, 14263"]
[]
["Supplementary Table S10"]
["Oligonucleotide primers"]
["RT-PCT primers*"]
["Gene","Forward (5’-to-3’)","Reverse (5’-to-3’)","CDNA SIZE (bp)#","GDNA SIZE (bp)#"]
["ACIN1","AGGAGGCTGTTGTGGATCTTCA","AGTTCGGACTGGGTCATCAATGG",347,1235]
["CDC6","GAGAACTAGCCAAAGTTCACCA","CTGGGCAGTCCTCAAGGACATG",392,2308]
["CHMP4B","CCCTCCAGGCACTGAAGCGTA","CGGGTTTTGATGGTAGGGCTAT",417,3734]
["CLASP1","TTCGACGATGAAGATTCTGTGGA","CTGTACTACAGGTACATCATCAA",219,7421]
["GOLGA5#1","TCCAAAGATCCAAAGAGACTCAAG","CTCCTGCTCTCTCTTCAGAGTG",353,3423]
["GOLGA5#2","GCACTCGTCTGCGAAATGTTCC","CTTCTTGCAGATCCCAAGTCTAG",335,3972]
["ITFG1","TGCTTACTGCTATAGCTCTCA","CAAGCCAGAATTTGTGTTTCAAC",204,3298]
["KIAA1715","CTGTTACTCCAGCCCTATCATCA","CCTTCAAAGCCATGCCATTATGAG",234,9224]
["MAPK1","CTGTAGGCTGCATTCTGGCA","CAATCCTCTTGTGTGGGTTGAATG",270,{"v":15849,"d":"15,849"}]
["MED1","GACTTCATTGCCAAAGTTGTTCAAAG","AAGGGCTGCTTCCATAAAGGGT",523,4827]
["NFAT5","CAGAACCTTTCCCAGGAAACTCA","GAAGTGGAGAAATTGAGTTCG",268,268]
["NFRKB","CAGAACCAGCTAAGTCTAGCT","TTTGACGGGCACAGTGGCCAT",323,1068]
["NMT1","CCCTGCTCTCGCAACTCAAG","TTCACAGGCTGATCCTGGGCT",259,{"v":20442,"d":"20,442"}]
["NVL","TGAGACGTGCGGGAAGGTTCGA","CTCTTAGCAACCCCAGCAGCCT",351,6374]
["PRPSAP2","GATGACATCATTGATGATGTTGACAG","GCCTCTGAAAGGATCATGCTGAT",239,1821]
["RBM14","GGCTGTCGCCATGTCGAAAAG","CGAATAGCGTGCGTAATCGGAA",166,948]
["RFX7","GCAATGGAGTGACTAATCTTCCT","TTGGAGAGCGAATGGTGAGTG",330,1790]
["SCD","CACTTGGGAGCCCTGTATGG","TGTTTGCGCACAAGCAGCCAA",323,6272]
["SGPL1","AGCTCACCAGGAAGATGCCCAT","TCCCCTCTTGCCAGAAGGCGT",204,6459]
["SUCLA2","CCAGGAGAGAATACTACTTTGCA","GTTTTCTGCTGCTGATTCCAC",230,19908]
["TM7SF3","GACAGTTACTGGTCCACAAGC","CTAGGGTCCAGAATGTTTGTCAC",263,1531]
["MED1 Sequencing primer","TACCATTGGCTCGAGTCCGA"]
[]
["gDNA PCR primers*"]
["CHMP4B","GATATGATACCTGTCCATTGC","CCAAATAGAGAAAGTGTCCCTC"," -",314]
["CLASP1","TTCGACGATGAAGATTCTGTGGA","GGGGACTGTATCACTTCTGAG"," -",250]
["GOLGA5#1","GAAACTGTACGTGCTCTGAGA","GTGGATACACTACAATGTTTACC"," -",354]
["ITFG1","ATCTGCTAATTGCTGGAGACTT","CAAGCCAGAATTTGTGTTTCAAC"," -",237]
["KIAA1715","GAATCTACTACAGAATCTACTAGC","CCTGTTTTGTGGACCATCACC"," -",351]
["MED1","CTCAGGTCTCATTCAGCAGCT","CTCAGGTGGTAATCTTGATGAC"," -",645]
["PRPSAP2","GATGACATCATTGATGATGTTGACAG","AGGCGTGAACCACTGCACCCA"," -",288]
["RFX7","CAGGCTAGACCTGTTAAATCTGA","TTGGAGAGCGAATGGTGAGTG"," -",279]
["SCD","CTAGCATCCAGAGAGTGTCT","ATAGTGTGGAGGACAAACAGCT"," -",258]
["TM7SF3","GGACTAACTCACCAGAAAGTC","CTAGGGTCCAGAATGTTTGTCAC"," -",277]
[]
["KIAA1715 In vitro RNA/DNA deamination primers"]
["KIAA1715-RNA(+T7)-F","TAATACGACTCACTATAGGGCTGTTACTCCAGCCCTATCATCA",null,null,405]
["KIAA1715-RNA-R","CTGATGAAAGCACACTTCCTG"]
["Sequencing primer for KIAA1715 cDNA ","TGCTACTTCAGTGCCTGGAATG"]
["MED1-T7-WT-F","TAATACGACTCACTATAGGGGGCTCGAGTCCGACCCCTCCT",null,null,236]
["MED1-M1-R1","CTTTTTCTTGGTCTTGTTGCTGGCCGAGC (mutated from CC in the WT MED1 RNA to GG)"]
["MED1-M1-R2","CTTTTTCTTGGTCTTGTTGCTGG"]
["Sequencing primer for ART MED1 cDNA ","T7 promoter"]
[]
[]
["A3G Site-directed mutagenesis primers"]
["Mutation","Primer 1 (5’-to-3’)^","Primer 2 (5’-to-3’)"]
["H65R (c.194 A>G)","CTTAAGTACCgCCCAGAGATG","TTCGGAATACACCTGGCC"]
["E67Q (c.199 G>C)","GTACCACCCAcAGATGAGATTC","TTAAGTTCGGAATACACCTG"]
["W94A (c.280-282 TGG>GCC)","GTACATATCCgccAGCCCCTGCAC","CAGGTGACCTCATACTCC"]
["C97S (c.289 T>A)","CTGGAGCCCCaGCACAAAGTG","GATATGTACCAGGTGACCTC"]
["C100S (c.298 T>A)","CTGCACAAAGaGTACAAGGGATATG","GGGCTCCAGGATATGTAC"]
["W127A (c. 379-381 TGG>GCC)","CTACTACTTCgccGACCCAGATTACCAGGAG","AGGCGGGCAACAAAGATG"]
["D128K (c.382-384 GAC>AAG)","CTACTTCTGGaagCCAGATTACCAG","TAGAGGCGGGCAACAAAG"]
["P129A (c.385 C>G)","CTTCTGGGACgCAGATTACCAG","TAGTAGAGGCGGGCAACA"]
["C291S (c.871-873 TGT>AGC)","CTGCTTCAGCagcGCCCAGGAAATG","GGGCTCCAGGAGGTGAAG"]
[]
[]
["*Underlined primers were used for Sanger sequencing"]
["# Expected amplicons sizes are determined by UCSC genome browser's In-Silico PCR tool"]
["^Nucleotides in lower case indicate designed mutations"]

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