Table S4. Alignments of the exon and splice donor sequences

1. uc001cjm.2_6_7

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
1	uc001cjm.2_6_7	chr1:43917099	-	GT	GC	(D4) shift; decrease; frameshift	alternative	HYI	NP_112484.3	NM_031207.5	putative hydroxypyruvate isomerase

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr1	43917099	43917215	-	GT	GGCATGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGAGCCCAGCAGCCCCGGAGAGCTGAATTTCCCCTATCTGTTTCAACTGCTGGAAGATGAAGGCTACAAAGGCTTCGTGGGCT
Chimp	panTro2	chr1	44149144	44149260	-	GC	GGCATGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGAGCCCAGCAGCCCCGGAGAGCTGAATTTCCCCTATCTGTTTCAACTGCTGGAAGATGAAGGCTACAAAGGCTTCGTGGGCT
Gorilla	gorGor1	Supercontig_0003107	28916	29032	-	GC	GGCATGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGAGCCCAGCAGCCCCGGAGAGCTGAATTTCCCCTATCTGTTTCAACTGCTGGAAGATGAAGGCTACAAAGGCTTCGTGGGCT
Orangutan	ponAbe2	chr1	186466683	186466799	+	GC	GGCATGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGAGCCCAGCAGCCCTGGAGAGCTGAATTTCCCCTATCTGTTTCAACTGCTGGAAGATGAAGGCTACAAAGGCTTTGTGGGCT
Rhesus	rheMac2	chr1	46359094	46359210	-	GC	GGCATGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGAGCCCAGCAGCCCCGGAGAGCTGAATTTCCCCTATCTGTTTCAACTGCTGGAAGATGAAGGCTACAAAGGCTTTGTGGGCT
Marmoset	calJac1	Contig119	654784	654900	-	GC	GGCACGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGAGCCCAGCAGCCCCGGAGAGCTGAACTTCCCCTATCTGTTTCAACTGCTGGAAGATGAAGGCTACAAAGGTTTTGTGGGCT
Tarsier	tarSyr1	scaffold_96244	290	406	+	GC	GACATGTGCAGGTGGCACAGGTCCCTGGCCGAGGGGAACCCAGCAGCCCTGGAGAGCTGAACTTCCCCTATCTATTTCAACTGCTGGAGGATGAAGGCTACAAAGGCTTTGTGGGTT
Lemur	micMur1	scaffold_754	241036	241152	-	GC	GGCACGTGCAAGTGGCGCAGGTCCCGGGCCGAGGGGAACCCAGCAGCCCTGGAGAGCTGAACTTCCCCTATCTGTTTCAACTGCTGGAAGACGAAGGCTACAAAGGCTTTGTGGGCT
Mouse	mm9	chr4	118034968	118035084	+	GC	GGCATGTGCAAGTGGCCCAGGTCCCAGACCGCGGGGAGCCAGGCAGCTCTGGAGAGCTAGACTTTACTTACCTGTTTCAGCTGCTAGAAGATGAGGGCTACCAAGGATTTGTGGGTT
Cow	bosTau4	chr3	109496466	109496582	+	GC	GGCATGTGCAGGTGGCACAGGTCCCGGGTCGGGGGGAACCCGATAGCCCCGGAGAGCTCAACTTCTCCTATCTGTTCCAACTGCTGGAAGATGAAGGCTACACAGGCTTCGTGGGCT
Dog	canFam2	chr15	19740393	19740509	+	GC	GGCACGTGCAGGTGGCACAGGTCCCGGGCCGAGGGGAGCCTGACAGCCCTGGAGAGCTGAACTTCCCCTATCTATTCCAACTGCTGGAGGACGAAGGCTACAAGGGCTTTGTGGGCT

Alignment (splice site sequences are in lowercase)

Human      agGGCATGTGCAGGTGGCACAGGTCCCAGGCCGAGGGGAGCCCAGCAGCCCCGGAGAGCTGAATTTCCCCTATCTGTTTC
Chimp      ................................................................................
Gorilla    ................................................................................
Orangutan  ...................................................T............................
Rhesus     ................................................................................
Marmoset   ......C........................................................C................
Tarsier    ...A.......................T...........A...........T...........C...........A....
Lemur      ......C.....A.....G........G...........A...........T...........C................
Mouse      ............A.....C..........A...C........AG.....T.T........AG.C..TA.T..C.......
Cow        ...........................G..T..G.....A...GAT..............C..C...T..........C.
Dog        ......C....................G..............TGA......T...........C...........A..C.

Human      AACTGCTGGAAGATGAAGGCTACAAAGGCTTCGTGGGCTgt
Chimp      ........................................c
Gorilla    ........................................c
Orangutan  ...............................T........c
Rhesus     ...............................T........c
Marmoset   ............................T..T........c
Tarsier    ..........G....................T.....T..c
Lemur      .............C.................T........c
Mouse      .G.....A........G......C....A..T.....T..c
Cow        ........................C...............c
Dog        ..........G..C...........G.....T........c

2. uc001gdg.2_1_6

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
2	uc001gdg.2_1_6	chr1:165601511	+	GT	AT	(D7) exonization; novel start	alternative	MGST3	Q5VV89	BG709310.1	microsomal glutathione S-transferase 3

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr1	165601477	165601511	+	GT	ATGCCCTGGAGGGGAAGGCGCTTCCGGCTCTGCAG
Chimp	panTro2	chr1	144961676	144961710	+	AT	ATGCCCTGGAGGGGAAGGCGCTTCCGGCTCTGCAG
Gorilla	gorGor1	Supercontig_0000869	26021	26055	-	AT	ATGCCCTGGAGGGGAAGGCGCTTCCGGCTCTGCAG
Orangutan	ponAbe2	chr1	85617990	85618024	-	AT	GTGCCCTGGAGGGGAAGGCGTTTCCGGCTCTGCAG
Rhesus	rheMac2	chr1	195474349	195474383	+	AT	GTGCCCTGGAGGGGAAGGCGCTTCCGGCTTTGCAG
Baboon	papHam1	scaffold12435	60180	60214	-	AT	GTGCCCTGGAGGGAAAGGCGCTTCCGGCTTTGCAG
Marmoset	calJac1	Contig2324	294359	294393	+	AT	GTGCCCTGAAGAGGAAGGCACTTCCGGCTCTGAAG
Mouse	mm9	chr1	169322903	169322937	-	CC	GTGCCCCAGAGAGCAACCAGCTCTCTGCTCTGTTG

Alignment (splice site sequences are in lowercase)

Human      ATGCCCTGGAGGGGAAGGCGCTTCCGGCTCTGCAGgt
Chimp      ...................................a.
Gorilla    ...................................a.
Orangutan  G...................T..............a.
Rhesus     G............................T.....a.
Baboon     G............A...............T.....a.
Marmoset   G.......A..A.......A............A..a.
Mouse      G.....CA...A.C..CCA...CT.T......TT.cc

3. uc010pkz.1_1_11

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
3	uc010pkz.1_1_11	chr1:165667645	-	GT	GC	(D3) shift; decrease; inframe	alternative	ALDH9A1	B4DX14	AK293520.1	aldehyde dehydrogenase 9 family, member A1

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr1	165667645	165667795	-	GT	ATGTTTCTCCGAGCAGGCCTGGCCGCGCTCTCCCCGCTTCTTCGCAGTCTTCGGCCCTCTCCTGTCGCCGCCATGAGCACTGGCACCTTCGTCGTGTCGCAGCCGCTCAATTACCGCGGCGGGGCCCGCGTGGAGCCGGCGGACGCCTCCG
Chimp	panTro2	chr1	145030074	145030224	-	GC	ATGTTTCTCCGAGCAGGCCTGGCCGCGCTCTCCCCGCTTCTTCGCAGTCTTCGGCCCTCTCCTGTCGCCGCCATGAGCACTGGCAACTTCGTCGTGTCGCAGCCGCTCAATTACCGCGGCGGGGCCCGCGTGGAGCCGGCGGACGCCTCCG
Gorilla	gorGor1	Supercontig_0208017	271	421	-	GC	ATGTTTCTCGGAGCAGGCCTGGCCGCGCTCTCCCCGCTTCTTCGCAGTCTTCGGCCCTCTTCTGTCGCCGCCATGAGCACTGGCACCTTCGTCGTGTCGCAGCCGCTCAATTACCGCGGCGGGGCCCGCGTGGAGCCGGCGGACGCCTCCG
Orangutan	ponAbe2	chr1	85547429	85547579	+	GC	ATGTTTCTCCGAGCAGGCCTGGCCGCGCTCTTCCCGCTTCTTCGCAGTCTTCGGCCCTCTTCTGTCGCCGCCATGAGCACTGGCACCTTCGTCGTGTCGCAGCCGCTCAATTACCGCGGCGGGGCCCGCGTGGAGCCGGCGGACGCCTCCG
Rhesus	rheMac2	chr1	195544970	195545120	-	GC	ATGTTTCTCCGAGCAGGCCGGGCCGCGCTCTCTCCGCTTGTTCGCAGTCTTCAGCCCTCTCCTGTCGCCGCCATGAGCACTGGCACCTTCGTCGTGTCGCAGCCGCTCAATTACCGCGGCGGGGCCCGCGTGGAGCCGGCTGACGCCTCCG
Baboon	papHam1	scaffold44409	316	466	+	GC	ATGTTTCTCCGAGCAGGCCTGGCCGCGCTTTCTCCGCTTGTTCGCAGTCTTCAGCCCTCTCCTGTCGCCGCCATGAGTACTGGCACGTTCGTCGTGTCGCAGCCGCTCAATTACCGCGGCGGGGCCCGCGTGGAGCCGGCTGACGCCTCCG
Marmoset	calJac1	Contig2324	360785	360935	-	GC	ATGTTTTTCCGAGCGGGCCGGGCTGCGGTTTCTTCGTTTCTTCGCAGTGTTCTGCAGTCTCCTGTGGCCACCATGAGCACCGGCACCTTCGTTGTGTCGCAGCCGCTTAATTACCGTGGCGGGACCCGCGTGGAGCCGGCGGACGCCTCTG
Mouse	mm9	chr1	169280367	169280517	+	GC	ATGATCCTCGGTGCAGTCGGTTCTGTGCTGACCTCGCTCCTCCGCATTCACCGGGCTGCAGCAGTCGCCGCCATGAGTACTGGCACCTTCGTCGTGTCGCAGCCGCTCAACTACCGCGGCGGGGCCCGTGTGGAGCCGGTGGATGCTTCCG

Alignment (splice site sequences are in lowercase)

Human      ATGTTTCTCCGAGCAGGCCTGGCCGCGCTCTCCCCGCTTCTTCGCAGTCTTCGGCCCTCTCCTGTCGCCGCCATGAGCAC
Chimp      ................................................................................
Gorilla    .........G..................................................T...................
Orangutan  ...............................T............................T...................
Rhesus     ...................G............T......G............A...........................
Baboon     .............................T..T......G............A........................T..
Marmoset   ......T.......G....G...T...G.T..TT..T...........G...T..AG........G...A..........
Mouse      ...A.C...G.T....T.GGTT.T.T...GA..T....C..C....T..AC...G.TG.AG.A..............T..

Human      TGGCACCTTCGTCGTGTCGCAGCCGCTCAATTACCGCGGCGGGGCCCGCGTGGAGCCGGCGGACGCCTCCGgt
Chimp      .....A..................................................................c
Gorilla    ........................................................................c
Orangutan  ........................................................................c
Rhesus     ............................................................T...........c
Baboon     ......G.....................................................T...........c
Marmoset   C...........T..............T........T......A.........................T..c
Mouse      ..............................C.................T..........T...T..T.....c

4. uc010pxj.1_9_10

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
4	uc010pxj.1_9_10	chr1:232144729	+	GT	GC	(D3) shift; decrease; inframe	alternative	DISC1	NP_001012975.1	NM_001012957.1	disrupted in schizophrenia 1 protein

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr1	232144531	232144729	+	GT	CTGCAAGTGTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTGCTTATCCAGAGCCTACAGCTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGAAGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGCTCCTCCTATTCCCCCCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGAAG
Chimp	panTro2	chr1	212644817	212645027	+	GC	CTGCAAGTGTCCACTGCTCGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTGCTTATCCAGAGCCTCCAACTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGAAGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGTCTGTATAGCTGCTCCTCCTATTCCCCCCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGCAG
Orangutan	ponAbe2	chr1	17691423	17691633	-	GC	CTGCAAGTGTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCAATTGCTTATCCAGAGCCTACAACTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGAAGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGCCTGTACAGCTGCTCCTCCCTTTCCCCCTAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGCAG
Rhesus	rheMac2	chr1	224948263	224948473	-	GC	CTGCAAGTGTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTGCTTATGCAGAGCCTACAACTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGAAGATGAGAGGCAGATGGATGCCTTAGAGGGAGCTGTTTGTATAGCTGCTTCTCCCATTACCCCCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGCAG
Baboon	papHam1	scaffold3058	89674	89884	+	GC	CTGCAAGTGTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTGCTTATCCAGAGCCTACAACTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGAAGATGAGAGGCAGATGGATGCCTTAGAGGGAGCTGTTTGTATAGCTGCTCCTCCCATTCCCCCCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGCAG
Marmoset	calJac1	Contig1169	330206	330416	-	GC	CTGCAAGTGTCCACTGCTTGGAAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTGCTTATCCAGAGGCTACAACTCCATGAAGCCAAGGGCAGCCTGTCTGTAGAAGATGAGGGGCAGATGGATGACCTAGTGGGAGCTGTCTGTTCAGCGGCTCCTCCCATTCCCCCTAGGCTCCACTCTGAGGATGAGAGGAAGACCCCTTTGCAG
Tarsier	tarSyr1	scaffold_184886	205	415	-	GC	CTGCAAGTATCCACTGCTTGGAAAAGTGTGGGAAGCTGACTTGGAAGCTTGCAGGTTGCTTATTCAGAGCCTGCAACTCCGGGAAGCCAGGGGCAGTCTGTCTGGCGAAGACCAGAGGCAGATGAATGACACAGAGGGAGCTGCCTGTACCACCACTCCAGCCATGCCCCTCAGGCTGCAGTCCCATGATGAGAGGAAGACTCATTTGCAG
Lemur	micMur1	scaffold_387	591459	591669	+	GC	CTGCACGTGTCCTCTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGGTTGCTTATCCAGAGCCTGCAACTCCAGGAGGCCAGGGGCAGCCTGTCTGTGGAAGATGAGAGGCAGGTGGAGGACATGGAGGGAGCCATCTGTGCAGCCACTGCAGCCGTCCACCCCAGGCCCCACTTCGGAGACGAGAGGAAGACCCCTTTGCAG
Galago	otoGar1	scaffold_109877.1-179029	79331	79541	-	GC	CTGCAAATGTTTGTTGCTTGGGAAAGTGTGGGAAGCAGACCTGGAAGCTTGTCGGTTGCTTATCCAGAGCCTGAACCTCCAGGAAGCCAGGGGCAGCCTGTCTGCAGAAGATGAGAGGCAGATGGATGACTTAGAGGGAGCCGTCTGTACAGCCTCTCTGGCCCTCCACCCCACACCCCACTCTGAAGATGAGACGAAGACTCCTTTGCAG
Mouse	mm9	chr8	127756684	127756891	+	GT	CTGCAGGTGCCCGCTGCTTGGGAGAGTGTGGAAAGCAGACTTGGAGACTTGTCAGTTGCTAATGCAGAGCCTGCAGCTTCAGGAAGCAGGCAGCAGCCCACACGCAGAGGACGAGGAGCAGGTGCATAGCACAGGAGAGGCCGCCCAGACAGCTGCTCTGGCTGTCCCTCGAACACCCCACCCCGAAGAAGAAAAGTCCCCCTTGCAG
Cow	bosTau4	chr28	2843477	2843684	+	GC	CTGCAAGTGTCCGCTGCTTGGAAAAGTATGGGAAGCTGACTTGGAAGCTTGTCGGTTGCTTATCCAGAGCTTGCAGCTCCAGGAGGCCAGGGGCAGCCTGTTTGCAGATGAGAGACAGACGGATGACTTAGGGGGGGGTACTTATACAATGGCTCTGGCCACCCCCCTGAGGTCCCACCTTGAAGATGGAAGGAAGAACCCTTTGCAA
Dog	canFam2	chr4	10553203	10553410	-	GC	CTGCAAGTGTCCACTGCTTGGAAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCAGTTGCTTATCCAGGGCCTACAGCTTCAGGCCAGGGGCATACTATCTGCAGAAGATGAGAGGCAGACAGATGACTTAGGGGGAGCTGCCTGTACATCCACTCCTGCCATCTCCCCCAGGCTCCACTCTGAAGTTGAGAAGAAGACCCCTTTGCAA

Alignment (splice site sequences are in lowercase)

Human      agCTGCAAGTGTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTGCTTATCCAGAGCCTACAGCT
Chimp      ....................C.....................................................C..A..
Orangutan  .......................................................A.....................A..
Rhesus     .................................................................G...........A..
Baboon     .............................................................................A..
Marmoset   .......................A...............................................G.....A..
Tarsier    ..........A............A.............................CA.G........T........G..A..
Lemur      .......C......T.........................................G.................G..A..
Galago     ........A...TTGT......................A...C.............G.................GA.C..
Mouse      .......G...C..G..........G.......A....A........GA......AG.....A..G........G.....
Cow        ..............G........A.....A..........................G...............T.G.....
Dog        .......................A...............................AG............G..........

Human      CCAGGAAGCCAGGGGAAGCCTGTCTGTAGAAGATGAGAGGCAGATGGATGACTTAGAGGGAGCTG------------CTC
Chimp      .................................................................TCTGTATAGCTG...
Orangutan  .................................................................CCTGTACAGCTG...
Rhesus     ..................................................C..............TTTGTATAGCTG..T
Baboon     ..................................................C..............TTTGTATAGCTG...
Marmoset   ...T.......A...C.....................G..............C...T........TCTGTTCAGCGG...
Tarsier    ..G............C..T.......GC.....CC...........A.....AC...........CCTGTACCACCA...
Lemur      ......G........C...........G...............G....G...A.G........CATCTGTGCAGCCA..G
Galago     ...............C..........C....................................C.TCTGTACAGCCT...
Mouse      T........AG.CA.C....CACAC.C...G..C...GA....G..C..AG.AC..GA.AG..C.CCCAGACAGCTG...
Cow        ......G........C.......T..C---.........A....C...........G...G.G.ACTTATACAATGG...
Dog        T...---........C.TA..A....C.................CA..........G........CCTGTACATCCA...

Human      CTCCTATTCCCCCCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGAAGgt
Chimp      ..................................................C...c
Orangutan  ....CT.......T....................................C...c
Rhesus     ....C...A.........................................C...c
Baboon     ....C.............................................C...c
Marmoset   ....C........T...........T......G.G...............C...c
Tarsier    .AG.C..G....T......G..G...C.T...G.G........T.A....C...c
Lemur      .AG.CG.C.A........C.....T..GA..CG.G...............C...c
Galago     TGG.CC.C.A.....CA.C......T..A...G.G.C......T......C...c
Mouse      TGG..G.C..T.GA.CA.C....C....A..AG..---...T....C...C....
Cow        TGG.C.CC....TG...TC....CTT..A...GG........A.......C.A.c
Dog        ..G.C..CT................T..A.T.G.G.A.............C.A.c

5. uc010pyf.1_1_9

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
5	uc010pyf.1_1_9	chr1:240408865	+	GT	AT	(D7) exonization; novel start	alternative	FMN2	B7Z4S3	AK297755.1	formin 2

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr1	240408863	240408865	+	GT	ATG
Chimp	panTro2	chr1	221136708	221136710	+	AT	ATG
Gorilla	gorGor1	Supercontig_0003929	6639	6641	+	AT	ATG
Orangutan	ponAbe2	chr1	9091502	9091504	-	AT	ATG
Rhesus	rheMac2	chr1	216798381	216798383	-	AT	ATG
Baboon	papHam1	scaffold28386	3802	3804	-	AT	ATG
Marmoset	calJac1	Contig5724	54384	54386	-	AT	ATG
Lemur	micMur1	scaffold_4942	480	482	+	AG	ACA
Galago	otoGar1	scaffold_114297.1-93085	42664	42666	+	AG	ATA
Mouse	mm9	chr1	176571332	176571334	+	GT	ATG
Dog	canFam2	chr7	34784602	34784604	+	AT	AAG

Alignment (splice site sequences are in lowercase)

Human      ATGgt
Chimp      ...a.
Gorilla    ...a.
Orangutan  ...a.
Rhesus     ...a.
Baboon     ...a.
Marmoset   ...a.
Lemur      .CAag
Galago     ..Aag
Mouse      .....
Dog        .A.a.

6. uc002tif.2_1_7

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
6	uc002tif.2_1_7	chr2:113514470	-	GT	CT	(D8) intronization; inframe	alternative	CKAP2L	Q8IYA6-2	AK097948.1	cytoskeleton associated protein 2-like

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr2	113514470	113514472	-	GT	ATG
Chimp	panTro2	chr2a	113682725	113682727	-	CT	ATG
Orangutan	ponAbe2	chr2a	20743087	20743089	-	CT	ATG
Rhesus	rheMac2	chr13	112122214	112122216	-	CT	ATG
Baboon	papHam1	scaffold1075	92707	92709	+	CT	ATG
Marmoset	calJac1	Contig3611	21797	21799	-	CT	ATG
Tarsier	tarSyr1	scaffold_3761	23945	23947	-	CT	ATG
Galago	otoGar1	scaffold_93571.1-228472	44303	44305	-	CT	CTG
Mouse	mm9	chr2	129111521	129111523	-	CT	ATG
Cow	bosTau4	chr11	48173516	48173518	-	CT	CTG
Dog	canFam2	chr17	40066396	40066398	-	CT	ATA

Alignment (splice site sequences are in lowercase)

Human      ATGgt
Chimp      ...c.
Orangutan  ...c.
Rhesus     ...c.
Baboon     ...c.
Marmoset   ...c.
Tarsier    ...c.
Galago     C..c.
Mouse      ...c.
Cow        C..c.
Dog        ..Ac.

7. uc010fqy.2_2_3

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
7	uc010fqy.2_2_3	chr2:177054409	+	GT	GC	(D6) exonization; frameshift	alternative	HOXD1	B2RAB4	BC028190.1	homeobox D1	dbSNP:rs13390932

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr2	177054277	177054409	+	GT	ACAGCGGTTTGGGTTTTGTGGAGGAGACGCGTTCCCGGGCGAATTCCATGGAGTCTGCCTCGAGTACAGATTCGGAAAATGTGCCAGGCATTGAGAAAATTTCAGGAAGGAGCTCTCCGTGGAACTTCTCCTG
Chimp	panTro2	chr2b	181195617	181195749	+	GC	ACAGCGGTTTGGGTTTTGTGGAGGAGACGCGTTCCCGGGCGAATTCCATGGAGTCTGCCTCGAGTACAGATTCGGAAAATGTGCCAGGCATTGAGAAAATTTCAGGAAGGAGCTCTCCGTGGAACTTCTCCTG
Gorilla	gorGor1	Supercontig_0413396	1690	1822	-	GC	ACAGCGGTTTGGGTTTGTGGAGGAGACGCNTTCCCGGGCGAATTCCACGGAGTCTGCCTCGAGTACAGATTCGGAAAATGTGCCAGGCATTGAGAAAATTTCAGGGAAGGAGCTCTCCGTGGAACTTCTCCTG
Orangutan	ponAbe2	chr2b	66346877	66347009	+	GC	ACAGCGGTTTGGGTTTTGTGGAGGAGACGCGTTCCCGGGCGAATTCCATTGAGTCTGCCTCGAGTACAGACTCGGAAAATGTGCCAGGCATTCAGAAAATTGCAGGAAGGAGCTCTCCGTGGAATGTCTCTTG
Rhesus	rheMac2	chr12	39831165	39831297	+	GC	ACAGCGGTTTGGGTTTTGTGGAGAAGACGCGTTCCTGGGCGAATTCCATTGAGTCTGCCTCGAGTACTGACTCGGAAAATGTGCCAGGCATTGAGAAAATTTCAGGAAGGAGCTCTCCGTGAAATTTCTCCTG
Baboon	papHam1	scaffold10146	794	926	-	GC	ACAGCGGTTTGGGTTTTGCGGAGAAGACACGTTCTTGGGCGAATTCCATTGAGTCTGCCTCGAGTACAGACTCGGAAAATGTGCCAGGCATTGAGAAAATTTCAGGAAGGAGCTCTCCGTGAAATTCCTCCTG
Marmoset	calJac1	Contig349	558227	558358	+	GC	ACAGCGGTTGGGGTTTTGTGGAGGAGACGCGCTCCCGGGCGAATTCCATTGAGCGTGCCTCGAGAACAGACTCGGAAAATGTGCCAGCCATTGAGAAAATTTCAGGAAGGAGCTCTCCGTGGAGTTTCTCTG
Lemur	micMur1	scaffold_2769	58411	58559	+	GC	ACAGCAGTTTGGTTTTAAGGAGGAAATGCGTTCCTGGGCGTTATTAAAGCAGGAGAATTCCATAGAGCATCCCCTGAGTCCCGGCTCAGAAAGCGTGCTAGGCATTGAGAAAATGACAGAAAGGAGCTCTTCGTGGAGCGCTTCTCCTG
Galago	otoGar1	scaffold_108759.1-101791	93392	93541	+	GC	ACAACAGTTTGGGTTTTATGCAGGAAATGCGTGCCTGGGCATTATTAAAGCAGGCGAATTTCATCGGGCGTGCCTCGAGTCCAGGCTCGGAAAACTTGCCAGGCACTTAGAAAATTATATAAAAGAACTTTCCACGGAGTGTTTCTCCTG

Alignment (splice site sequences are in lowercase)

Human      agACAGCGGTTTGGGTTTTGTGGAGGAGACGCGTTCCCGGGC---------------GAATTCCATGGAGTCTGCCTCGA
Chimp      ..........................................---------------.......................
Gorilla    ...............-................N.........---------------........C..............
Orangutan  ..........................................---------------.........T.............
Rhesus     .........................A...........T....---------------.........T.............
Baboon     ....................C....A....A.....TT....---------------.........T.............
Marmoset   ...........G.....................C........---------------.........T...CG........
Lemur      .......A....-......AA......A.T.......T....GTTATTAAAGCAGGA.........A...CA.C..CT..
Galago     .t...A.A...........A..C....A.T....G..T....ATTATTAAAGCAGGC.....T...C.G.CG........

Human      GTACAGATTCGGAAAATGTGCCAGGCATTGAGAAAATTTCA-GGAAGGAGCTCTCCGTGGA--ACTTCTCCTGgt
Chimp      .........................................-...................--...........c
Gorilla    .........................................G...................--...........c
Orangutan  .......C.....................C........G..-...................--.TG....T...c
Rhesus     ....T..C.................................-.................A.--.T.........c
Baboon     .......C.................................-.................A.--.T.C.......c
Marmoset   .A.....C................C................-...................--GT.....-...c
Lemur      ..C.C.GC..A....GC....T...............GA..-.A..........T......GCG..........c
Galago     ..C...GC........CT.........C.T........AT.-TA..A..A..T...AC...GTGT.........c

8. uc010zhi.1_1_13

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
8	uc010zhi.1_1_13	chr2:201728824	-	GT	GC	(D7) exonization; novel start	alternative	CLK1	NP_001155879.1	NM_001162407.1	CDC-like kinase 1

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr2	201728824	201728949	-	GT	ATGGCGGCTGGGCGGAGGCCGGCTTCGGCCCTGTGGCCGGAAAGGCGAGGCTCCCCGTTGAGGGGGGATTTGCTGGGGTTCCAGAATGTGCGTGAGCCAAGCAGCTGTGGGGAAACGTTGTCTGGA
Chimp	panTro2	chr2b	206307627	206307752	-	GC	ATGGCGGCTGGGCGGAGGCCGGCTTCGGCCCTGTGGCCGGAAAGGCGAGGCTCCCCGTTGAGGGGGGGTTTGCTGGGGTTCCAGAATGTGCGTGAGCCACGCAGCTGTGGGGAAACGTTGTCTGGA
Gorilla	gorGor1	Supercontig_0002069	14986	15111	-	GC	ATGGCGGCTGGGCGGAGGCCGGCTTCGGCCCTGTGGCCGGAAAGGCGAGGCTCCCCGTTGAGGGGGTGTTTGCTGGGGTTCCAGAATGTGCATGAGCCACGCAGCTGTGGGGAAACGTTGTCTGGA
Orangutan	ponAbe2	chr2b	92118000	92118126	-	GC	ATGGCGGCTGGGCGGAGGCCCGCTTCGGCCCTGTGGCCGGAAAGGCGAGGCTCCCTGTTGGGGGTGGGGTTTGCTGGGGTTCCAGAATGTGCGTGAGCTGCGCAGCTGAGGGGAAACGTTGTCTGGA
Rhesus	rheMac2	chr12	64616938	64617064	-	GC	ATGGCGGCTGGGCGGAGGCCGGCCTTGGCACTGTGGCCGGAAAGGCGAGGCTCCCTGTTGGGGGTGGGGTTTGCTGGGGTTCCGGAATGTGCTTGAAGCACGCAGCTGTGGGGAAATGTCGTCTGGA
Baboon	papHam1	scaffold30412	16833	16959	-	GC	ATGGCGGCTGGGCGGAGGCCGGCCTTGGCACTGTGGCCGGAAAGGCGAGGCTCCCTGTTGGGGGTGGGGTTTGCTGGGGTTCCGGAATGTGCTTGAAGCACGCAGCTGTGGGGAAATGTCGTCTGGA
Marmoset	calJac1	Contig1570	16440	16552	-	GC	ATGGCGGCTGGGCGGAGGCCGGCCTTGGTCACTGGGCGGAAAGGCGAGGCTCCCTGTTGGGGGTGGGGTTTGTTGGGATTCCAGAAAATCGCCGCTGTGGGGAAATGTCTGCG

Alignment (splice site sequences are in lowercase)

Human      ATGGCGGCTGGGCGGAGGCCGGCTTCGGCCCTGTGGCCGGAAAGGCGAGGCTCCCCGTTGAGGG-GGGATTTGCTGGGGT
Chimp      ................................................................-...G...........
Gorilla    ................................................................-..TG...........
Orangutan  ....................C..................................T....G...T...G...........
Rhesus     .......................C.T...A.........................T....G...T...G...........
Baboon     .......................C.T...A.........................T....G...T...G...........
Marmoset   .......................C.T..T.AC-...G..................T....G...T...G....T....A.

Human      TCCAGAATGTGCGTGAGCCAAGCAGCTGTGGGGAAACGTTGTCTGGAgt
Chimp      ....................C...........................c
Gorilla    ............A.......C...........................c
Orangutan  ..................TGC.......A...................c
Rhesus     ...G........T...AG..C...............T..C........c
Baboon     ...G........T...AG..C...............T..C........c
Marmoset   .......AA.----------C..C............---......CG.c

9. uc002vmt.1_9_10

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
9	uc002vmt.1_9_10	chr2:223066131	-	GT	AT	(D9) intronization; frameshift	alternative	PAX3	NP_852124.1	NM_181459.3	paired box 3

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr2	223066131	223066161	-	GT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Chimp	panTro2	chr2b	228182873	228182903	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Gorilla	gorGor1	Supercontig_0549285	766	796	+	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Orangutan	ponAbe2	chr2b	114209032	114209062	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Rhesus	rheMac2	chr12	86071397	86071427	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Baboon	papHam1	scaffold8480	13779	13809	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Marmoset	calJac1	Contig3263	136828	136858	+	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Tarsier	tarSyr1	scaffold_9940	4108	4138	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Lemur	micMur1	scaffold_863	289901	289931	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Galago	otoGar1	scaffold_109389.1-239627	38001	38031	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Mouse	mm9	chr1	78099380	78099410	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATTGC
Cow	bosTau4	chr2	114994349	114994379	-	AT	GTGCCTTTCATTATCTCAAGCCAGATATCGC
Dog	canFam2	chr37	31348626	31348656	-	CT	GTGCCTTTCATTATCTCAAGCCAGATATCGC

Alignment (splice site sequences are in lowercase)

Human      agGTGCCTTTCATTATCTCAAGCCAGATATCGCgt
Chimp      .................................a.
Gorilla    .................................a.
Orangutan  .................................a.
Rhesus     .................................a.
Baboon     .................................a.
Marmoset   .................................a.
Tarsier    .................................a.
Lemur      .................................a.
Galago     .................................a.
Mouse      ..............................T..a.
Cow        .................................a.
Dog        .................................c.

10. uc011axk.1_2_17

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
10	uc011axk.1_2_17	chr3:33118586	-	GT	AT	(D5) exonization; inframe	alternative	GLB1	B7Z6Q5	AK300753.1	galactosidase, beta 1

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr3	33118586	33118729	-	GT	TCTCGATTCCTTCCTTGGGCTTTTCATCTGCCACGGCAAGCGCCTAAGTCTCAGCTGCCGCTTCACAAAAGAGGTACCAAGACTGCACCGAATGAGCACGCAAGCAGCAACAGGAGTGGGAGAAGAAGGAGGCGGCAGCAGTGG
Chimp	panTro2	chr3	33872359	33872502	-	AT	TCTTGATTCCTTCCTTGGGCTTTTCATCTGCCACGGCAAGCGCCTAAGTCTCAGCTGCCGCTTCACAAAAGAGGTACCAAGACTGCACCGAATGAGCACGCAAGCAGCAACAGGAGTGGGAGAAGGAGGAGGCGGCAGCAGTGG
Gorilla	gorGor1	Supercontig_0005127	23443	23586	-	AT	TCTTGATTCCTTCCTTGGGCTTTTCATCTGCCACGGCAAGCGCCTAAGTCTCAGCTGCCGCTTCACAAAAGAGGTACCAAGACTGCACCGAATGAGCACGCAAGCAGCAACAGGAGTGGGAGAAGAAGGAGGCGGCAGCAGTGG
Orangutan	ponAbe2	chr3	113864078	113864221	+	AT	TCTTGATTCCTTCCTTGGGCTTTTCGTCTGCCACGGCAAGCACCTGAGTCTCAGCTGCCGCTTCACAAAAGAGGTACCAAGACTGCACCAAATGAGCATGCAAGCAGCAACAGGAGTGGGAGAAGAAGGAGGCGGCAGCAGTGG
Rhesus	rheMac2	chr2	175563822	175563965	-	AT	TCTTGATTCTTTCCTTGGGCTTTTTGTCTGCCATGGCAGGCGCCGGAGTCTCAGCTGCCGCTTCACAAAAGAGGTACCAAGTCTGCACCGAATGAGTACATAAGCAGCACCAGGAGTGGAAGAAGAAGGAGGCGGCAGCGGTGG
Baboon	papHam1	scaffold2033	183208	183351	+	AT	TCTTGATTCTTTCCTTGGGCTTTTTGTCTGCCATGGCAGGCGCCGGAGTCTCAGCTGCCGCTTCACAAAAGAGGTACCAAGTCTGCACCGAATGAGTACATAAGCAGCACCAGGAGAGGAAGAAGAAGGAGGCGGCAGCGGTGG

Alignment (splice site sequences are in lowercase)

Human      agTCTCGATTCCTTCCTTGGGCTTTTCATCTGCCACGGCAAGCGCCTAAGTCTCAGCTGCCGCTTCACAAAAGAGGTACC
Chimp      .....T..........................................................................
Gorilla    .....T..........................................................................
Orangutan  .....T.....................G...............A...G................................
Rhesus     .....T.....T..............TG.......T....G.....GG................................
Baboon     .....T.....T..............TG.......T....G.....GG................................

Human      AAGACTGCACCGAATGAGCACGCAAGCAGCAACAGGAGTGGGAGAAGAAGGAGGCGGCAGCAGTGGgt
Chimp      ...............................................G..................a.
Gorilla    ..................................................................a.
Orangutan  ...........A........T.............................................a.
Rhesus     ...T..............T..AT........C.........A...................G....a.
Baboon     ...T..............T..AT........C......A..A...................G....a.

11. uc011bql.1_1_17

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
11	uc011bql.1_1_17	chr3:180633316	+	GT	GG	(D7) exonization; novel start	alternative	FXR1	B4DXZ6	AK302197.1	fragile X mental retardation, autosomal homolog 1

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr3	180633305	180633316	+	GT	ATGGTCACTGAG
Chimp	panTro2	chr3	186336886	186336897	+	GG	ATGGTCACTGAG
Gorilla	gorGor1	Supercontig_0019635	7658	7669	+	GG	ATGGTCACTGAG
Orangutan	ponAbe2	chr3	184639389	184639400	+	GG	ATGGTCACTGAG
Rhesus	rheMac2	chr2	105814365	105814376	-	GG	ATGGTCACTGAG
Baboon	papHam1	scaffold3875	59827	59838	+	GG	ATGGTCACTGAG
Tarsier	tarSyr1	scaffold_15029	616	627	+	GG	ACGATCAGGGAA
Lemur	micMur1	scaffold_16499	4965	4976	+	GG	GTGGTTAGTGAA
Dog	canFam2	chr34	17141587	17141598	+	GG	GTGGTCAGTGAG

Alignment (splice site sequences are in lowercase)

Human      ATGGTCACTGAGgt
Chimp      .............g
Gorilla    .............g
Orangutan  .............g
Rhesus     .............g
Baboon     .............g
Tarsier    .C.A...GG..A.g
Lemur      G....T.G...A.g
Dog        G......G.....g

12. uc011cfo.1_2_11

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
12	uc011cfo.1_2_11	chr4:109683977	-	GT	GA	(D2) shift; increase; frameshift	alternative	AGXT2L1	NP_001140099.1	NM_001146627.1	alanine-glyoxylate aminotransferase 2-like 1

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr4	109683977	109683977	-	GT	A
Chimp	panTro2	chr4	112188172	112188172	-	GA	A
Orangutan	ponAbe2	chr4	113396428	113396428	-	GA	A
Rhesus	rheMac2	chr5	101674608	101674608	-	GA	A
Baboon	papHam1	scaffold22475	31476	31476	-	GA	A
Marmoset	calJac1	Contig5520	56017	56017	+	GA	A
Tarsier	tarSyr1	scaffold_14592	25640	25640	-	GC	G
Cow	bosTau4	chr6	17968714	17968714	+	GA	A

Alignment (splice site sequences are in lowercase)

Human      gaAgt
Chimp      ....a
Orangutan  ....a
Rhesus     ....a
Baboon     ....a
Marmoset   ....a
Tarsier    ..G.c
Cow        ....a

13. uc003ldj.2_17_18

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
13	uc003ldj.2_17_18	chr5:138268647	+	GT	GG	(D6) exonization; frameshift	alternative	CTNNA1	P35221-2	U03100.1	catenin (cadherin-associated protein), alpha 1, 102kDa

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr5	138268577	138268647	+	GT	AACTGTGACACCTGCGGGGCACTGCAAGGGCTGAAAGGCTGGCCTCCTCCCCTTTGCTGGCCACTCACTGG
Chimp	panTro2	chr5	140668915	140668985	+	GG	AACTGTGACACCTGGGGGGCACTGCAAGGGCTGAAAGGCTGGCCTCCTCCCCTTTGCTGGCCGCTCACTGG
Orangutan	ponAbe2	chr5	140491878	140491948	+	GA	AACTGTGACACCTGGGGGGCACTGCAGGGGCTGAAAGGCTGGCCGCCTCCCCTTTGCTGGCCACTCACTGG
Rhesus	rheMac2	chr6	135350520	135350590	+	GA	AACTGTGACACGTGTGGGCCACTGCAAGGGCTGAAAGGCTGGCCTCCTCCCCTTTGCTGGCCACTCACTGG
Baboon	papHam1	scaffold1426	129075	129145	+	GA	AACTGTGACACGTGCGGGCCACTGCAAGGGCTGAAAGGCTGGCCTCCTCCCCTTTGCTGGCCACTCACTGG
Marmoset	calJac1	Contig665	128601	128661	+	CA	AACTGTGATCATTGCAAGGGCTGAAAGGCTGGCTCCCTCCCCTTTGCTGGCCACTCACTGG

Alignment (splice site sequences are in lowercase)

Human      agAACTGTGACACCTGCGGGGCACTGCAAGGGCTGAAAGGCTGGCCTCCTCCCCTTTGCTGGCCACTCACTGGgt
Chimp      ................G...............................................G.........g
Orangutan  ................G...........G.................G...........................a
Rhesus     .............G..T...C.....................................................a
Baboon     .............G......C.....................................................a
Marmoset   ..........T----------..T.....................TC..........................ca

14. uc011dbw.1_12_13

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
14	uc011dbw.1_12_13	chr5:146763517	+	GT	AT	(D4) shift; decrease; frameshift	alternative	STK32A	B7Z9H7	AK315942.1	serine/threonine-protein kinase 32A

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr5	146763453	146763517	+	GT	AGTAAACAGGGACTTTAACAAAAGACAACCAAATCTAGCCTTGGAACAAACCAAAGACCCACAAG
Chimp	panTro2	chr5	149311251	149311315	+	AT	AGTAAACAGGGACTTTAACAAAAGACAACCAAATCTAGCCTTGGAACAAACCAAAGACCCACAAG
Gorilla	gorGor1	Supercontig_0000921	16959	17023	+	AT	AGTAAACAGGGACTTTAACAAAAGACAACCAAATCTAGCCTTGGAACAAACCAAAGACCCACAAG
Orangutan	ponAbe2	chr5	149092632	149092696	+	AT	AGTAAAAAGGGACTTTAACAAAAGACAACCAAATCTAGCCTTGGAACAAACCAAAGACCCACAAG
Rhesus	rheMac2	chr6	143841020	143841084	+	AT	AGTAAAAAGGGACTTTAACAAAAGACAACCAAATCTAACCTTGGAACAAACCAAAGACCCACAAA
Baboon	papHam1	scaffold3125	125202	125266	-	AT	AGTAAAAAGGGACTTTAACAAAAGACAACCAAATCTAACCTTGGAACAAACCAAAGACCCACAAA
Marmoset	calJac1	Contig231	456749	456813	+	AT	AGTAAAAAGGGACTTTAATAAAAGACAACCAAATCTAGCCTTGGAACAAACCAAAGACCCACAAG
Tarsier	tarSyr1	scaffold_16673	20845	20909	+	AG	AGTAAAAAGGGACTTTAATAAAAAGCAAGCAGACCTAGCCTCGGAACAAACCAAAGACCCACAAG
Lemur	micMur1	scaffold_1779	239748	239812	+	AA	AGTAAAAAGGGACTTTAATAAAAGGCCAGCAAATCTAGCCTTGGAACAAACCAAAGACCCACAAG
Galago	otoGar1	scaffold_96106.1-140986	68083	68147	+	GA	AGTAAAAAAGGACATTACTAAAAAGCAAGCACATCTAGCCTTGGAACAAACCAAAGACCCACAAG
Cow	bosTau4	chrUn.004.7	772996	773060	-	GA	AGTCAAAAGGGACTTTAATAAAAGACAAGCAAATCAAGCCTTGGAACAAACCAAAGACCCAGAAG
Dog	canFam2	chr2	44530855	44530919	+	AA	AGTAAAAAGGGACTTTACTAAAAGACCAGCTAACCTAGCTCTGGAACAAACCAAAGACCCACAAG

Alignment (splice site sequences are in lowercase)

Human      agAGTAAACAGGGACTTTAACAAAAGACAACCAAATCTAGCCTTGGAACAAACCAAAGACCCACAAGgt
Chimp      ...................................................................a.
Gorilla    ...................................................................a.
Orangutan  ........A..........................................................a.
Rhesus     ........A..............................A..........................Aa.
Baboon     ........A..............................A..........................Aa.
Marmoset   ........A...........T..............................................a.
Tarsier    ........A...........T....AG...G..G.C.......C.......................ag
Lemur      ........A...........T.....G.C.G....................................aa
Galago     ........A.A....A...CT....AG...G..C..................................a
Cow        .....C..A...........T.........G......A.........................G....a
Dog        ........A..........CT.......C.G.T..C.....TC........................aa

15. uc003pne.3_14_15

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
15	uc003pne.3_14_15	chr6:90338942	+	GT	GC	(D3) shift; decrease; inframe	alternative	ANKRD6	NP_055757.3	NM_014942.4	ankyrin repeat domain-containing protein 6

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr6	90338831	90338942	+	GT	ATATCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCAGAAGCTTTCTGGAGATTCTAGGGCCTGCAGAGCTAAATCCACACCATCTACTT
Chimp	panTro2	chr6	90886765	90886876	+	GC	ATATCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCAGAAGCTTTCTGGAGATTCTAGGGCCTGCAGAGCTAAATCCACACCATCTACTT
Gorilla	gorGor1	Supercontig_0048960	13459	13570	+	GC	ATATCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCAGAAGCTTTCTGGAGATTCTAGGGCCTGCAGAGCTAAATCCACACCATCTACTT
Orangutan	ponAbe2	chr6	90593512	90593623	+	GC	ATATCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCAGAAGCTTTCTGGAGATTCTAGAGCCTGCAGAGCTAAATCCACACCGTCTACTT
Rhesus	rheMac2	chr4	85489977	85490088	+	GC	ATCTCCTTGGTGGATGAATTAAAAACTTGGTGCATGTTAAAGATTCAGAATCTGGAGCAGAAGCTTTCTGGAGATTCTAGGGCCTGCAGAGCTAAATCCACACCATCTACTT
Baboon	papHam1	scaffold21683	3049	3160	+	GC	ATCTCCTTGGTGGATGAATTAAAAACTTGGTGCATGTTAAAGATTCAGAATCTGGAGCAGAAGCTTTCTGGAGATTCTAGGGCCTGCAGAGCTAAATCCACACCATCTACTT
Marmoset	calJac1	Contig44	453429	453540	+	GC	ATATCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCTGAAGCTTTCTGGAGATTCTAGGGCCTCCAGAGCTAAATCCACACCATCTACTT
Lemur	micMur1	scaffold_2057	140957	141068	+	GC	ACATCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCTGAAGCTTTCTGGAGATTCTAGAGCCTCCAGGGCTAAATCCACACCATCTACTT
Mouse	mm9	chr4	32895674	32895785	-	CT	ATGTCTTTGGTAGATGAACTAAAAGCCTGGTGCATGTTGAAGATCCAGAGTCTGGAGCTAAGACTCTCTGGAGAGTCTCGGACCTTCAGAGCTAAATCCACACCACCTCCGT
Cow	bosTau4	chr9	63226762	63226873	-	GC	ATGTCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCTGAAGCTTTCTGGAGATTCTAGGGCCTCCAGGACTAAATCCACACCGTCCACTT
Dog	canFam2	chr12	51842219	51842330	+	GC	ATGTCATTAGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCTGAAGCTTTCTGGAGATTCTAGGGCCTCTAGGACCAAATCCACACCATCTACTT

Alignment (splice site sequences are in lowercase)

Human      agATATCCTTGGTGGATGAATTAAAAACCTGGTGCATGTTAAAGATTCAGAATCTGGAGCAGAAGCTTTCTGGAGATTCT
Chimp      ................................................................................
Gorilla    ................................................................................
Orangutan  ................................................................................
Rhesus     ....C.......................T...................................................
Baboon     ....C.......................T...................................................
Marmoset   ............................................................T...................
Lemur      ...C........................................................T...................
Mouse      ....G..T.....A......C.....G.............G.....C....G........TA.GA..C........G...
Cow        ....G.......................................................T...................
Dog        ....G..A..A.................................................T...................

Human      AGGGCCTGCAGAGCTAAATCCACACCATCTACTTgt
Chimp      ...................................c
Gorilla    ...................................c
Orangutan  ..A.......................G........c
Rhesus     ...................................c
Baboon     ...................................c
Marmoset   .......C...........................c
Lemur      ..A....C...G.......................c
Mouse      C..A...T...................C..C.G.c.
Cow        .......C...GA.............G..C.....c
Dog        .......CT..GA.C....................c

16. uc011kma.1_7_8

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
16	uc011kma.1_7_8	chr7:107254177	+	GT	AT	(D6) exonization; frameshift	alternative	BCAP29	NP_001008405.1	NM_001008405.2	B-cell receptor-associated protein 29

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr7	107254099	107254177	+	GT	CATTCCAGTTTTGGTGAATTTTTAAGCAAAAGAAGCCACAAAAATGGCAGCATTGGAAAACAGACTGGTTCTAGAAAAG
Chimp	panTro2	chr7	107565757	107565839	+	AT	CATTCCAGTTTTGGTGAATTTTTAAGCAAAAGAATTAAGCCACAAAAATGGCAGCATTGGAAAACAGACTGGTTCTAGAAAAG
Orangutan	ponAbe2	chr7	103782359	103782441	+	AT	CATTCCAGTTTTGGTGAATTTTTAAGCAAAAGAATTAAGCCACAAAAATGGCAGCATTGGAAAACAGACTGGTTCTAGAAAAG
Rhesus	rheMac2	chr3	145037787	145037869	+	AT	CATTCCAGTTTTGGTGAACTTTTAAGCAAAAGAATTAAGCCACAAAAATGGCAGCACTGGAAAACAGACGGGTTCTAGAAAAG
Baboon	papHam1	scaffold4836	11208	11290	+	AT	CATTCCAGTTTTGGTGAACTTTTAAGCAAAAGAATTAAGCCACAAAAATGGCAGCACTGGAAAACAGACGGGTTCTAGAAAAG
Marmoset	calJac1	Contig2609	211589	211671	-	TT	CATTCCAGTTTTAGTGAATCTTTAAGCAAAAGAAGTAAGCCACAAAAATGGCAGCATTGGAAAACAGACTGGTTCTAGAAGAG
Lemur	micMur1	scaffold_1185	102388	102470	+	TT	CATTCCAGATTTGGTGAAAGTTTAACTCAAAGAATTGATCCACAAAACTGGAAGCATTGAAAAAGATACTAGTTCTAGAAAAA
Galago	otoGar1	scaffold_94154.1-94172	87428	87510	-	TT	CATTCCAGATTTGGTGAAGGCTTAAGCAAAAAAATTGAACCACAAAACCAACAGCATTGAAAAAGAGACTAGTTCTAGAAAAA
Dog	canFam2	chr18	15962859	15962941	-	TT	TGTTCTAGTTCTAGTGAAATTCTAAGCAAAAGAATTGAGCCACAAAAATGGCAGCATTGAAAAATGGGCTGTTTCTGTAAACA

Alignment (splice site sequences are in lowercase)

Human      agCATTCCAGTTTTGGTGAATTTTTAAGCAAAAGA----AGCCACAAAAATGGCAGCATTGGAAAACAGACTGGTTCTAG
Chimp      ...................................ATTA.........................................
Orangutan  ...................................ATTA.........................................
Rhesus     ....................C..............ATTA...................C............G........
Baboon     ....................C..............ATTA...................C............G........
Marmoset   ..............A......C.............AGTA.........................................
Lemur      ..........A.........AG.....CTC.....ATTG.T........C...A.......A....G.T...A.......
Galago     ..........A.........GGC..........A.ATTG.A........CCAA........A....G.....A.......
Dog        t.TG...T....C.A.....A..C...........ATTG......................A....TG.G...T....GT

Human      AAAAGgt
Chimp      .....a.
Orangutan  .....a.
Rhesus     .....a.
Baboon     .....a.
Marmoset   ..G..t.
Lemur      ....At.
Galago     ....At.
Dog        ...CAt.

17. uc003wii.2_2_10

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
17	uc003wii.2_2_10	chr7:150725998	+	GT	TT	(D5) exonization; inframe	alternative	ABCB8	Q6ZRM3	AK128129.1	ATP-binding cassette, sub-family B (MDR/TAP), member 8

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr7	150725888	150725998	+	GT	GAGGCTCCTGCGTCTGCAGCCGCGTGTCAGCCAGAAGGAGGGGACGCTCGGGGTCAGTGACCACGCCCAGTCCGCACTCCCGACCGGGGGTCCCCTTTCCTGGCCCTGGAG
Chimp	panTro2	chr7	151562169	151562279	+	TT	GAGGCTCCTGCGTCTGCAGCCGCGTGTCAGCCAAAAGGAGGGGACGCTCGGGGTCAGTGACCACGCCCAGTCCGCACTCCCGACCAGGGGTCCCCTTTCCTGGCCCTGGAG
Gorilla	gorGor1	Supercontig_0007123	33062	33172	-	TT	GAGGCTCCTGCGTCTGCAGCCGCGTGTCAGCCAGAAGGAGGGGACGCTCGGGGTCAGTGACCACGCCCAGTCCGCACTCCCGACCAGGGGTCCCCTTTCCTGGCCCTGGAG
Orangutan	ponAbe2	chr7	148712369	148712479	+	TT	GAGGCTCCTGCGTCTGCAGCCGCGTGTCAGCCAGAAGGAGGGGACGCTCGGGGTCAGTGACCACGCCCAGTCAGCACTCCCGACCAGGGGTCGCCTTTCCTGGCCCTGGAG
Rhesus	rheMac2	chr3	187973971	187974081	+	TT	GAGGTTCCGGCGTCTGCATCGGCGTGTCAGCCAGAAGGAGGGAACGCTCCGGGTCTGTGACCACGCCCAGTCCGCACTCTCGACCAGGGGTCGCCTTTCCTGGACCCCGAG
Baboon	papHam1	scaffold4613	132098	132208	-	TT	AAGGTGCCGGCGTCTGCATCGGCGTGTCAGCCAGAAGGAGGGAACGCTCCGGGTCTGTGACCACGCCCAGTCCGCACTCTCGACCAGGGGTCGGCTTTCCTGGACCCCGAG

Alignment (splice site sequences are in lowercase)

Human      agGAGGCTCCTGCGTCTGCAGCCGCGTGTCAGCCAGAAGGAGGGGACGCTCGGGGTCAGTGACCACGCCCAGTCCGCACT
Chimp      ...................................A............................................
Gorilla    ................................................................................
Orangutan  ..........................................................................A.....
Rhesus     ......T...G.........T.G.....................A......C.....T......................
Baboon     ..A...TG..G.........T.G.....................A......C.....T......................

Human      CCCGACCGGGGGTCCCCTTTCCTGGCCCTGGAGgt
Chimp      .......A.........................t.
Gorilla    .......A.........................t.
Orangutan  .......A......G..................t.
Rhesus     .T.....A......G..........A..CC...t.
Baboon     .T.....A......GG.........A..CC...t.

18. uc003wjw.2_11_14

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
18	uc003wjw.2_11_14	chr7:151071287	+	GT	GC	(D3) shift; decrease; inframe	alternative	NUB1	NP_057202.3	NM_016118.4	negative regulator of ubiquitin-like proteins 1

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr7	151071183	151071287	+	GT	GAACTGGCCCAAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCGCCTCGAGAACATCAGGTTTCTGAAAGGGATGGGCTACTCCACGCACGCGGCCCAGCAG
Chimp	panTro2	chr7	151916784	151916888	+	GC	GAACTGGCCCAAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCGCCTGGAGAACATCAGGTTTCTGAAAGGGATGGGTTACTCCACGCACGCGGCCCAGCAG
Gorilla	gorGor1	Supercontig_0005020	7567	7671	+	GC	GAACTGGCCCAAATAAGGATGGAGGAAAAAGAGAAGAAAAGACGCCGCCTCGAGAACATCAGGTTTCTGGAAGGGATGGGCTACTCCACGCACGCGGCCCAGCAG
Orangutan	ponAbe2	chr7	149040268	149040372	+	GC	GAACTGGCCCAAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCGCCTCGAGAACATCAGGTGTCTGAAAGGGATGGGCTACTCCACGCACGCGGCCCAGCAG
Rhesus	rheMac2	chr3	188328069	188328173	+	GC	GAATTGGCCCAAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCGCCTCGAGAACATCAGGTCTCTGAAAGGGATGGGCTACTCCACGCACGCAGCCCAGCAG
Baboon	papHam1	scaffold1041	24977	25081	-	GC	GAATTGGCCCAAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCGCCTCGAGAACATCAGGTCTCTGAAAGGGATGGGCTACTCCACGCACGCAGCCCAGCAG
Marmoset	calJac1	Contig3894	216054	216158	+	GC	GAACTGGCCCAAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCACCTGGAGAAGATCAGGTCCCTGAAAGGGATGGGCTACTCCACGCACGCAGCCCAGCAG
Tarsier	tarSyr1	scaffold_70333	5770	5874	+	GC	GAATTGGCCCAAATAAGGAAGGAAGAAAAAGAGAAGAAAAGAAACCGCCTGGAAAACATCAACGCCCTGAAGGTGATGGGCTACTCCACGCAGGCAGCCAAGCAG
Galago	otoGar1	scaffold_73616.1-11485	10469	10572	+	GC	GAATTGGCCCAAATAAGGAAAGAGGAAAAGAGAAGAAAAGACGCCGCCTGGAGAACATCAACTCTCTGAAGTGCATGGGCTACTCCACACACGCAGCCAAGCAG
Mouse	mm9	chr5	24212256	24212360	+	GC	GAACTGGCCCAAATCCGGAAGGAAGAGAAGGAGAAAAGGAGACGCCGTCTGGAAAATGTCAACACTTTGAGAGGAATGGGCTACTCCACACAAGCTGCCAAACAA
Cow	bosTau4	chr4	118220992	118221096	+	GC	GAACTGGACCAAATAAAGAAGGAGGAAAGAGAGAAGAAAAAACGCCGCCTGGAGAACATTAACCATTTGAAAGGAATGGGCTACTCCATGCGAGCAGCCAGGCAG
Dog	canFam2	chr16	18325869	18325972	+	GC	GAATTGGCCCAATAATGAAAGAGGAAAAAGAGAAGAAAAGACGCCGGCTGGAGAACATAAACTCCTTGAAAGGAATGGGCTACTCTACTCATGCAGCCAAGCAG

Alignment (splice site sequences are in lowercase)

Human      agGAACTGGCCCAAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCGCCTCGAGAACATCAGGTTTCTGAAAGGGATG
Chimp      ....................................................G...........................
Gorilla    .....................T.................................................G........
Orangutan  ..................................................................G.............
Rhesus     .....T............................................................C.............
Baboon     .....T............................................................C.............
Marmoset   ................................................A...G.....G.......CC............
Tarsier    .....T...................A..................AA......G..A.......ACGCC.....G.T....
Galago     .....T................A.....-.......................G..........AC.C......GT.C...
Mouse      ................CC.......A..G..G.....A.GG........T..G..A..TG...ACAC.T...G...A...
Cow        .........A........A...........G...........A.........G........T.ACCA.T.......A...
Dog        .....T........-...T...A..........................G..G........A.AC.CCT.......A...

Human      GGCTACTCCACGCACGCGGCCCAGCAGgt
Chimp      ..T.........................c
Gorilla    ............................c
Orangutan  ............................c
Rhesus     .................A..........c
Baboon     .................A..........c
Marmoset   .................A..........c
Tarsier    ..............G..A...A......c
Galago     ...........A.....A...A......c
Mouse      ...........A..A..T...A.A..A.c
Cow        ..........T..GA..A...AG.....c
Dog        ........T..T..T..A...A......c

19. uc003ybd.2_5_9

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
19	uc003ybd.2_5_9	chr8:79601608	+	GT	AT	(D1) shift; increase; inframe	constitutive	FAM164A	NP_057094.2	NM_016010.2	family with sequence similarity 164, member A	GTNGTN

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr8	79601457	79601608	+	GT	ATTATATTCAATGTCCATATTGTCAGAGGAGATTCAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGGAAATTTTCTACAGATACCAAAGGAAAACCAACTTCTCGGACACAGGTG
Chimp	panTro2	chr8	77244221	77244372	+	AT	ATTATATTCAATGTCCATATTGTCAGAGGAGATTCAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGGAAATTTTCTACAGATACCAAAGGAAAACCAACTTCTCGGACACAGGTG
Gorilla	gorGor1	Supercontig_0250583	4671	4822	+	AT	ATTATATTCAATGTCCATATTGTCAGAGGAGATTCAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGGAAATTTTCTACAGATACCAAAGGAAAACCAACTTCTCGGACACAGGTG
Orangutan	ponAbe2	chr8	81520068	81520219	+	AT	ATTATATTCAATGTCCATATTGTCAGAGGAGATTCAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGGAAATTTTCTACAGATACCAAAGGAAAACCAACTTCTCGGACACAGGTG
Rhesus	rheMac2	chr8	81143885	81144036	+	AT	ATTATATTCAGTGTCCATATTGTCAGAGGAGATTCAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGCAAATTTTCTACAGATACCAAAGGAAAACCAACTTCTCGGACACAGGTG
Baboon	papHam1	scaffold11214	63688	63839	-	AT	ATTATATTCAGTGTCCATATTGTCAGAGGAGATTCAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGCAAATTTTCTACAGATACCAAAGGAAAACCAACTTCTCGGACACAGGTG
Marmoset	calJac1	Contig9735	32637	32788	+	AT	ATTATATTCAGTGTCCATATTGTCAGAGGAGATTTAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGCAAATATTCTACAGATACCAAAGGAAAACCAACGTCTCGGACACAGGTG
Cow	bosTau4	chr14	38934552	38934703	+	AT	ACTATATTCAGTGTCCATATTGCCAGAGAAGATTTAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGCAAATTTTCTACTGATACCAAAGGAAAATCAACCTCTCGGACACAGGTA
Dog	canFam2	chr29	29340088	29340239	+	AA	ATTATATTCAGTGTCCATATTGCCAGAGGAGATTTAATGAAAATGCAGCTGATAGACATATTAACTTCTGTAAAGAACAGGCAGCACGTATTAGTAATAAAGGCAAATTTTCTACTGACACCAAAGGAAAGGCATCTTCTCGGACACAGGTG

Alignment (splice site sequences are in lowercase)

Human      agATTATATTCAATGTCCATATTGTCAGAGGAGATTCAATGAAAATGCAGCTGATAGACATATAAATTTCTGTAAAGAAC
Chimp      ................................................................................
Gorilla    ................................................................................
Orangutan  ................................................................................
Rhesus     ............G...................................................................
Baboon     ............G...................................................................
Marmoset   ............G.......................T...........................................
Cow        ...C........G...........C.....A.....T...........................................
Dog        ............G...........C...........T..........................T..C.............

Human      AGGCAGCACGTATTAGTAATAAAGGGAAATTTTCTACAGATACCAAAGGAAAACCAACTTCTCGGACACAGGTGgt
Chimp      ..........................................................................a.
Gorilla    ..........................................................................a.
Orangutan  ..........................................................................a.
Rhesus     .........................C................................................a.
Baboon     .........................C................................................a.
Marmoset   .........................C....A...........................G...............a.
Cow        .........................C...........T...............T....C..............Aa.
Dog        .........................C...........T..C...........GG..T.................aa

20. uc003yil.1_11_12

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
20	uc003yil.1_11_12	chr8:99205613	-	GT	AT	(D6) exonization; frameshift	alternative	NIPAL2	NP_079035.1	NM_024759.1	NIPA-like domain containing 2	dbSNP:rs3735887

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr8	99205613	99205640	-	GT	ACACAACCCCAGAGAGAAAGGCTTGGAG
Chimp	panTro2	chr8	97120943	97120970	-	AT	ACACAACCCCAGAGAGAAAGACTTGGAG
Gorilla	gorGor1	Supercontig_0003142	14453	14480	+	AT	ACACAACCCCAGAGAGAAAGGCTTGGAG
Orangutan	ponAbe2	chr8	104573089	104573116	-	AT	ACACAACCCCAGAGAGAAAGGCTTGGAG
Rhesus	rheMac2	chr8	100737464	100737491	-	AT	ACACAACCCTGGGGAGAAAGGCTTGGAG
Baboon	papHam1	scaffold7311	93298	93325	-	AT	ACACAACCCTGGGGAGAAAGGCTTGGAG
Cow	bosTau4	chr14	64224490	64224517	+	AT	ACAGAACAGCGGAGAGAGAGGTCTGGAG
Dog	canFam2	chr13	3429240	3429267	-	AT	AAAGGACCCCAGGAAGGAAGGTGTGGAG

Alignment (splice site sequences are in lowercase)

Human      agACACAACCCCAGAGAGAAAGGCTTGGAGgt
Chimp      ......................A.......a.
Gorilla    ..............................a.
Orangutan  ..............................a.
Rhesus     ...........TG.G...............a.
Baboon     ...........TG.G...............a.
Cow        .....G...AG.G......G...TC.....a.
Dog        ...A.GG.......GA..G....TG.....a.

21. uc001ljt.2_45_52

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
21	uc001ljt.2_45_52	chr10:129216805	+	GT	GC	(D10) type change (GC to GT)	constitutive	DOCK1	NP_001371.1	NM_001380.3	dedicator of cytokinesis 1	GC to GT

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr10	129216629	129216805	+	GT	GTGGAAATCAGCCCCCTGGAGAATGCCATTGAGACCATGCAGCTGACGAACGACAAGATCAACAGCATGGTGCAGCAGCACCTGGATGACCCCAGCCTGCCCATCAACCCGCTCTCCATGCTCCTGAACGGCATCGTGGACCCAGCTGTCATGGGGGGCTTCGCAAACTACGAAAAG
Chimp	panTro2	chr10	128497849	128498025	+	GC	GTGGAAATCAGCCCCCTGGAGAATGCCATCGAGACCATGCAGCTGACGAACGACAAGATCAACAGCATGGTGCAGCAGCACCTGGATGACCCCAGCCTGCCCATCAACCCGCTCTCCATGCTCCTGAACGGCATTGTGGACCCAGCTGTCATGGGGGGCTTCACAAACTACGAAAAG
Orangutan	ponAbe2	chr10	127063741	127063917	+	GC	GTGGAAATCAGCCCCCTGGAGAATGCTATCGAGACCATGCAGCTGACGAACGACAAGATCAACAGCATGGTACAGCAGCACCTGGATGACCCCAGCCTGCCCATCAACCCGCTCTCCATGCTCCTGAATGGCATCGTGGACCCAGCTGTCATGGGGGGCTTCACAAACTACGAAAAG
Rhesus	rheMac2	chr9	127050562	127050738	+	GC	GTGGAAATCAGCCCCCTGGAGAACGCCATCGAGACCATGCAGCTGACGAACGACAAGATCAACAGCATGGTGCAGCAGCACCTGGATGACCCCAGCCTGCCCATCAACCCACTCTCCATGCTCCTGAACGGCATCGTGGACCCAGCTGTCATGGGGGGCTTCACAAACTACGAAAAG
Baboon	papHam1	scaffold2063	130725	130901	-	GC	GTGGAAATCAGCCCCCTGGAGAACGCCATCGAGACCATGCAGCTGACGAACGACAAGATCAACAGCATGGTGCAGCAGCACCTGGATGACCCCAGCCTGCCCATCAACCCGCTCTCCATGCTCCTGAACGGCATCGTGGACCCAGCTGTCATGGGGGGCTTCACAAACTACGAAAAG
Marmoset	calJac1	Contig3078	214365	214541	+	GC	GTGGAAATTAGCCCCCTGGAGAACGCCATCGAGACCATGCAGCTGACAAATGACAAGATCAACAGCATGGTGCAGCAGCACCTGGACGACCCCAGCCTGCCCATCAACCCGCTCTCCATGCTCCTGAACGGCATCGTGGACCCAGCTGTCATGGGGGGCTTCACAAACTACGAAAAG
Lemur	micMur1	scaffold_164	727334	727510	+	GC	GTGGAGATCAGCCCCCTGGAGAACGCCATCGAGACCATGCAGCTGACAAACGACAAGATCGACAGCATGGTGCAGCAGCACCTGGACGACCCCAGCCTCCCTGTCAACCCCCTCTCCATGCTGCTTAACGGAATCGTGGACCCTGCCGTTATGGGGGGCTTTGCAAACTACGAAAAG
Galago	otoGar1	scaffold_103683.1-619930	121077	121253	+	GC	GTGGAGGTCAGCCCCCTGGAGAACGCCATAGAGACCATGCAACTGACCAATGACAAGATCGACAGCATGGTGCAGCAGCACCTGGATGACCCCAGCCTGCCCATCAACCCCCTGTCCATGCTCCTCAACGGCATCGTGGACCCCGCTGTCATGGGAGGATTCGCAAACTATGAAAAG
Mouse	mm9	chr7	142337097	142337273	+	GC	GTAGAGATCAGCCCACTGGAGAATGCCATCGAGACAATGCAGTTGACCAATGACAAGATCAGCAGCATGGTCCAGCAGCACCTGGATGACCCGGGCCTGCCTATCAACCCCCTGTCCATGCTCCTGAATGGCATTGTGGATCCTGCTGTCATGGGTGGTTTTGCCAATTATGAGAAG
Cow	bosTau4	chr26	47680961	47681137	+	GC	GTGGAGATCAGCCCCCTGGAGAACGCCATCGAGACCATGCAGCTGACCAACGACAAGATCAACAGCATGGTCCAGCAGCACCTAGACGACCCCAGCCTCCCCATCAACCCGCTCTCCATGCTCCTAAACGGCATCGTGGACCCCGCCGTCATGGGAGGTTTTGCCAATTACGAGAAG
Dog	canFam2	chr28	39370692	39370868	+	GT	GTGGAGATCAGCCCCCTGGAGAATGCCATCGAGACCATGCAGCTGACCAACGACAAGATGAACAGCATGGTGCAGCAGCACCTGGACGACCCCAGCCTCCCCATCAACCCCCTCTCCATGCTCCTCAATGGCATCGTGGACCCCGCCGTCATGGGAGGCTTTGCAAATTATGAAAAG

Alignment (splice site sequences are in lowercase)

Human      agGTGGAAATCAGCCCCCTGGAGAATGCCATTGAGACCATGCAGCTGACGAACGACAAGATCAACAGCATGGTGCAGCAG
Chimp      ...............................C................................................
Orangutan  ............................T..C.........................................A......
Rhesus     .........................C.....C................................................
Baboon     .........................C.....C................................................
Marmoset   ..........T..............C.....C.................A..T...........................
Lemur      .......G.................C.....C.................A............G.................
Galago     .......GG................C.....A...........A.....C..T.........G.................
Mouse      ....A..G........A..............C.....A......T....C..T..........G.........C......
Cow        .......G.................C.....C.................C.......................C......
Dog        .......G.......................C.................C...........G..................

Human      CACCTGGATGACCCCAGCCTGCCCATCAACCCGCTCTCCATGCTCCTGAACGGCATCGTGGACCCAGCTGTCATGGGGGG
Chimp      ........................................................T.......................
Orangutan  ..................................................T.............................
Rhesus     ................................A...............................................
Baboon     ................................................................................
Marmoset   ........C.......................................................................
Lemur      ........C...........C..TG.......C...........G..T.....A...........T..C..T........
Galago     ................................C..G...........C.................C...........A..
Mouse      ..............GG.......T........C..G..............T.....T.....T..T...........T..
Cow        .....A..C...........C..........................A.................C..C........A..
Dog        ........C...........C...........C..............C..T..............C..C........A..

Human      CTTCGCAAACTACGAAAAGgt
Chimp      ....A...............c
Orangutan  ....A...............c
Rhesus     ....A...............c
Baboon     ....A...............c
Marmoset   ....A...............c
Lemur      ...T................c
Galago     A...........T.......c
Mouse      T..T..C..T..T..G....c
Cow        T..T..C..T.....G....c
Dog        ...T.....T..T........

22. uc001lvx.1_6_7

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
22	uc001lvx.1_6_7	chr11:2334938	+	GT	GC	(D4) shift; decrease; frameshift	alternative	TSPAN32	Q96QS1-3	AF176070.1	tetraspanin-32

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr11	2334884	2334938	+	GT	GTGGAGGACGCCATGCTGGACACCTACGACCTGGTATATGAGCAGGCGATGAAAG
Chimp	panTro2	chr11	2368438	2368492	+	GC	GTGGAGGACGCCATGCTGGACACCTACGACCTGGTATATGAGCAGGCGATGAAAG
Orangutan	ponAbe2	chr11	3598089	3598143	+	GC	GTGGAGGATGCCATGCTGGACACCTACGACCTGGTATATGAGCAGGCGATGAAAG
Rhesus	rheMac2	chr14	2321288	2321342	+	GC	GTGGAGGATGCCATGCTGGACACCTACGACCTGGTATATGAGCAGGCGATGAAAG
Baboon	papHam1	scaffold5713	527	581	-	GC	GTGGAGGATGCCATGCTGGACACCTACGACCTGGTATATGAGCAGGCAATGAAAG
Marmoset	calJac1	Contig11729	63051	63105	-	GC	GTTGAGGATGCCATGCTGGACACCTACGACCTGGTGTATGAGCAGGCAGTGAAAG
Galago	otoGar1	scaffold_94756.1-68377	8045	8099	+	CT	GTGGAGGATGCTGCTTTGGATACCTACGACCTGGTATATGAACAAGCGGTGAAGA
Mouse	mm9	chr7	150200864	150200918	+	GC	GTGGAGGATGCGGTGTTGGACACCTATGACTTCGTGTATGATCAGGCAATGAAGA
Cow	bosTau4	chr29	51123701	51123755	-	GC	ATGGAGGACGCCGTGCTGGATGCCTATGACCGGGCCTATGAGCGGGCGCTGAGGA
Dog	canFam2	chr18	49457421	49457475	+	AC	GTGGAGGATGCTGTTTTGGACACTTACGACCTGGTGTATGACCAGGCGGTGAAGA

Alignment (splice site sequences are in lowercase)

Human      agGTGGAGGACGCCATGCTGGACACCTACGACCTGGTATATGAGCAGGCGATGAAAGgt
Chimp      ..........................................................c
Orangutan  ..........T...............................................c
Rhesus     ..........T...............................................c
Baboon     ..........T......................................A........c
Marmoset   ....T.....T..........................G...........AG.......c
Galago     ..........T..TGCTT....T....................A..A...G....GAc.
Mouse      ..........T..GG..T..........T...T.C..G.....T.....A.....GA.c
Cow        ..A...........G.......TG....T....G..CC.......G....C...GGA.c
Dog        ..........T..TG.TT.......T...........G.....C......G....GAac

23. uc009ywj.2_1_19

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
23	uc009ywj.2_1_19	chr11:94225808	-	GT	AT	(D1) shift; increase; inframe	alternative	MRE11A	B3KTC7	AK095388.1	meiotic recombination 11 homolog A (S. cerevisiae)	dbSNP:rs496797

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr11	94225808	94225836	-	GT	ATGAACAGAAATATTAGTCATCAAAAAGG
Chimp	panTro2	chr11	93012501	93012529	-	AT	ATCAACAGAAATATTAGTCATCAAAAAGG
Gorilla	gorGor1	Supercontig_0009178	19023	19051	+	AT	ATCAACAGAAATATTAGTCATCAAAAAGG
Orangutan	ponAbe2	chr11	90464221	90464249	-	AT	ATGAACAGAAATATTAGTCATCAAAAAGG
Rhesus	rheMac2	chr14	93041985	93042013	-	AT	ATGAACAGAAATACTAGTCATCAAAAAGG
Baboon	papHam1	scaffold11054	29745	29773	-	AT	ATGAACAGAAATACTAGTCATCAAAAAGG
Marmoset	calJac1	Contig3955	19968	19996	+	AT	GTGAACAGAAATATTAGTCATCAAAAAGG
Lemur	micMur1	scaffold_11751	28191	28219	-	AT	ATGAACAGGAATGTGAGTCATCAAAAAGG
Dog	canFam2	chr21	9450035	9450063	+	AT	ATGAACAGAAACGTTCGTCATCAAAAAGA

Alignment (splice site sequences are in lowercase)

Human      ATGAACAGAAATATTAGTCATCAAAAAGGgt
Chimp      ..C..........................a.
Gorilla    ..C..........................a.
Orangutan  .............................a.
Rhesus     .............C...............a.
Baboon     .............C...............a.
Marmoset   G............................a.
Lemur      ........G...G.G..............a.
Dog        ...........CG..C............Aa.

24. uc001qip.3_20_26

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
24	uc001qip.3_20_26	chr12:999676	+	GT	AT	(D3) shift; decrease; inframe	alternative	WNK1	NP_055638.2	NM_014823.2	serine/threonine-protein kinase WNK1	GTNGTN

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr12	999619	999676	+	GT	CCTGTGTCCATGGCGGCTCCAACAGCAATCACAGAAGCAGGAACACAGCCTCAGAAGG
Chimp	panTro2	chr12	913688	913745	+	AT	CCTGTGTCCGTGGTGGCTCCAGCAGCAATTACAGAAGCAGGAGCACAGCCTCAGAAGG
Orangutan	ponAbe2	chr12	899525	899582	+	AT	CCTGTGCCCGTGGTGGTTCCAACAGCAATTACAGAAGCAGGAGCACAGCCTCAGAAGG
Rhesus	rheMac2	chr11	881860	881917	+	AT	CCTGTGTCCGTGGTGGCTCCAGCAGCAGTTACAGAAGCAGGAGCACAGCCTCAGAAGG
Baboon	papHam1	scaffold108	105965	106022	-	AT	CCTGTGTCCGTGGTGGCTCCAGCAGCAGTTACAGAAGCAGGAGCACAGCCTCAGAAGG
Marmoset	calJac1	Contig707	362457	362514	-	AT	CCTGTGTCCGTGGTGGCTCCAACAGCAGTTACAGAAGCAGGAGCACAGCCTCAGAAGG
Tarsier	tarSyr1	scaffold_6429	43022	43079	+	AT	CCTGTGTCCGTGGTGGCTCCAACAGCGGGTACGGAAGCAGGAGCACCGCTTCAAAAGG
Lemur	micMur1	scaffold_6516	28599	28656	+	AT	CTGGTGTCCATGGTGGCTCCAACAGCAGTTACAGAAGCAGGAGCACAGCCTGAGAAGG
Galago	otoGar1	scaffold_103064.1-155269	16633	16690	+	AT	CCTGTGTCCATGGTGGCTCCAATAGCTGTTACAGAATCAGGAGCACAGCCTCAGAAGG
Mouse	mm9	chr6	119893827	119893884	-	AT	CCTTTGTCTACGATGTCTTCAACAACAGTTACAGAAGCAGGGACTCGGCTTCAAAAGG
Cow	bosTau4	chr5	114602325	114602382	+	AT	CCCGTGTCTGTGGTGGCTCCAACAGCAGTTGTGGAGACCGAAGCCCAACTTCCAAAGG
Dog	canFam2	chr27	45929131	45929188	+	AT	CCTGTGTCCATGGTGGCTCCAACAGCAGTCACAGAAGCAGGAGCACAGCCTCAGAAGG

Alignment (splice site sequences are in lowercase)

Human      agCCTGTGTCCATGGCGGCTCCAACAGCAATCACAGAAGCAGGAACACAGCCTCAGAAGGgt
Chimp      ...........G...T.......G.......T............G...............a.
Orangutan  ........C..G...T..T............T............G...............a.
Rhesus     ...........G...T.......G.....G.T............G...............a.
Baboon     ...........G...T.......G.....G.T............G...............a.
Marmoset   ...........G...T.............G.T............G...............a.
Tarsier    ...........G...T............GGGT..G.........G...C..T...A....a.
Lemur      ...TG..........T.............G.T............G........G......a.
Galago     ...............T........T...TG.T......T.....G...............a.
Mouse      .....T....T.C.AT.T..T.....A..G.T...........G..T.G..T...A....a.
Cow        ....C.....TG...T.............G.TGTG..GA.C.A.G.C..A.T..CA....a.
Dog        ...............T.............G..............G...............a.

25. uc001szm.1_1_16

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
25	uc001szm.1_1_16	chr12:81472207	+	GT	TT	(D3) shift; decrease; inframe	alternative	ACSS3	Q9H6R3	DA156034.1	acyl-CoA synthetase short-chain family member 3	GTNGTN

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr12	81471900	81472207	+	GT	ATGAAACCGTCTTGGCTGCAGTGTCGTAAAGTCACCAGCGCCGGGGGGCTCGGAGGGCCCTTGCCTGGGTCCTCTCCGGCCCGGGGAGCCGGTGCGGCCCTCAGGGCTTTAGTGGTCCCGGGCCCGCGGGGCGGTCTCGGGGGCCGGGGATGCAGGGCACTGTCCTCCGGCAGTGGCAGCGAGTACAAGACCCACTTCGCAGCCTCGGTGACCGACCCCGAGAGGTTCTGGGGCAAAGCTGCCGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAAACACTCGCCCTCTACCAG
Chimp	panTro2	chr12	81728625	81728932	+	TT	ATGAAACCGTCTTGGCTGCAATGTCGTAAAGTCACCAGCGCCGGGGGGCTCGGAGGACCCTTGCCTGGGTCCTCTCCGGCCCGGGGAGCCGGTGCGGCCCTCAGGGCTTTAGTGGTCCCGGGCCCGCGGGGCGGTCTCGGGGGCCGGGGATGCAGGGCACTGTCCTCCGGCAGTGGCAGCGAGTACAAGACCCACTTCGCAGCCTCGGTGACCGACCCCGAGAGGTTCTGGGGCACAGCTGCCGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAAACACTCGCCCTCTACCAG
Gorilla	gorGor1	Supercontig_0155513	18955	19262	-	TT	ATGAAACCGTCTTGGCTGCAATGTCGTAAAGTCACCAGCGCCGGGGGGCTCGGAGGGCCCTTGCCTGGGTCCTCTCCGGCCCGGGGAGCCGGTGCGGCCCTCAGGGCTTTAGTGGTCCCGGGCCCGCGGGGCGGTCTCGGGGGCCGGGGATGCAGGGCACTGTCCTCCGGCAGTGGCAGCGAGTACAAGACCCACTTCGCAGCCTCGGTGACCGACCCCGAGAGGTTCTGGGGCAAAGCTGCCGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAAACACTCGCCTTCTACCAG
Orangutan	ponAbe2	chr12	81619645	81619952	+	TT	ATGAAACCGTCTTGGCTGCAATGTCATAAAGTCACCAGCGCCGGGGGGCTCGGAGGGCCCTTGCCTGGGTCCTCTCCGGCCCGGGGAGCCAGTGCGGCCCTCAGGGCTTTAGTGGTCCCGGGCCCGCGGGGCGGTCTCGGGGGCCGGGGATGCAGGGCGCTGTCCTCCGGCAGTGGCAGCGAGTACAAGACCCACTTCGCAGCGTCGGTGACCGACCCGGAGAGGTTCTGGGGCAAAGCTGCCGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAAACACTCGCCCTCTACCAG
Rhesus	rheMac2	chr11	78037995	78038302	+	TT	ATGAAACCCTCTTGGCTGCAATGTCGTAAAGTCACCGGCGCCGGGGGGCTCGGAGGGACCTTGCCTGGGTCCTCTCCGGCCCGGGGAGCCGGTGCGGCCCTCAGGGCTTTAGTggccccgggcccgcggggcggTCTTGGAGGTCGGGGCTGCAGGGCGCTGTCCTCCGGCAGTGGCAGCGAGTACAAGACCCACTTCACAGCCTCGGTGACCGACCCCGAGAGGTTCTGGGGCAAAGCTGCAGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAAACACTCGCCCTCTACCAG
Baboon	papHam1	scaffold24807	13102	13409	+	TT	ATGAAACCCTCTTGGCTGCAATGTCGTAAAGTCACCGGCGCCGGGGGGCTCGGAGGGACCTTGCCTGGGTCCTCTCCGGCCCGGGGAGCCGGTGCGGCCCTCAGGGCTTTAGTGGCCCCAGGCCCGCGGGGCGGTCTCGGAGGTCGGGGCTGCAGGGCGCTGTCCTCCGGCAGTGGCAGCGAGTACAAGACCCACTTCACAGCCTCGGTGACCGACCCCGAGAGGTTCTGGGGCAAAGCTGCTGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAAACACTCGCCCTCTACCAG
Marmoset	calJac1	Contig4152	128699	129006	-	TT	ATGAAACCGTCTTGGCTGCAATACCGTAAAGTCACCGGCGCCGGGGGGCTCGGAGGGCCTTTGCCCGGGTCTTCTGCGGCCCGGGGAGCCGGTGCGGCCCGCAGGGCTTTCGTGGCCCCGGGTCCGCGGGGTGGTCTCGGGACCCTGGGCTGCAGAGCGCTGTCCTCGGGCAGTGGCAGCGAGTACAAGACCCACTTCGCCGCCTCGGTGACGGACCCCGAGAGGTTCTGGGGCAAAGCTGCTGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAAACACTCGCCCTCCACCAG
Tarsier	tarSyr1	scaffold_19051	14794	15101	+	TT	ATGAAACCGTCTTGGCTGCAGTATCGCAAAGTCACCGGCGCCGGGGGCCTCGGGGGTCCCCTGCCCGGGTCCTCTGCGGCCAGGGGCGCCGGAGCGGCCCTCAGGGCGTACGTGGCTCCCGGCCCCCGGGCTGGCCTCGGGGGCCGGGGCTGCAGGGCGCTGTCCTCAGGCAGCGGCGGCGAGTACAGGACCCACTTCGCAGCCTCGGTGGGCGACCCCGAGAGGTTCTGGGGCAAAGCTGCGGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAGACACCCGCCTTTCACCAG
Lemur	micMur1	scaffold_2130	179455	179762	+	TT	ATGAAACCGTCTTGGCTACAATGTCGCAAAGTCAccggcgccgggggcctcggggggcccctgcccgggTCCCCTCCAGCCCGGGGAGCCGGTGCGGCCCGCAGGGCTTTGGTGGCCCCGGGCCCGTGGGGCGCTGTCGGGGGCCGGGGCTGCAGGGCGCTGGCCTCGGGCAGCGTGGGCGAGTACAAGACGCACTTCGCGGCCTCGCTGGCCGACCCCGAGAGGTTCTGGGGCAAAGCTGCCGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTAGAGAACAGACACCCTCCTTCCACCAG
Mouse	mm9	chr10	106560347	106560639	-	TT	ATGAAGCCATCCTGGTTGCAATGTCGCAAAGTAACAGGCGCCGGGACGCTTGGGGCACCTCTGCCAGGATCCCCTTCAGTAAGAGGAGCCGCTGTGACCCGAAGGGCTTTGGTGGCCGGGTTTGGGGGCAGGGGCTGCAGGGCTCTGACCACCGGCAGTGGTGGTGAGTACAAGACCCACTTCGCAGCCTCTGTGGCTGACCCCGAAAGATTCTGGGGTAAAGCTGCTGAGCAGATCAGTTGGTACAAGCCCTGGACCAAAACTCTAGAGAGCAGATACCCGCCTTCCACAAG
Cow	bosTau4	chr5	12742310	12742614	+	TT	ATGAAGCCGTCTTGGCTGCAGTGTCGCAAAGTCACCGGCGCGGGGGGCCTCGGCGGGTCCCTGCCCGCGTCCTCTcccgcgcgcggagccggcccggcccgccgggcgtacgtggcccccggcccgcggggcgctctcgggggccggggcTGCAGGGCGCTGTCCTCGGGCGGCGGCGAGTACAAGACCCACTTCGCGGCCTCGGTGACCGACCCCGAGAGGTTCTGGGGCAAAGCCGCGGAGCAGATCAGCTGGTACAAGCCCTGGACCAAGACGCTGGAGAACAGACATTCGCCTTCCACCAG

Alignment (splice site sequences are in lowercase)

Human      ATGAAACCGTCTTGGCTGCAGTGTCGTAAAGTCACCAGCGCCGGGGGGCTCGGAGGGCCCTTGCCTGGGTCCTCTCCGGC
Chimp      ....................A...................................A.......................
Gorilla    ....................A...........................................................
Orangutan  ....................A....A......................................................
Rhesus     ........C...........A...............G....................A......................
Baboon     ........C...........A...............G....................A......................
Marmoset   ....................A.AC............G......................T.....C.....T...G....
Tarsier    ......................A...C.........G..........C.....G..T...C....C.........G....
Lemur      .................A..A.....C.........G..........C.....G......C....C......C....A..
Mouse      .....G..A..C...T....A.....C.....A..AG........AC...T..G.CA..TC....A..A...C..T.A.T
Cow        .....G....................C.........G....G.....C.....C...T..C....C.C.........C..

Human      CCGGGGAGCCGGTGCGGCCCTCAGGGCTTTAGTGGTCCCGGGCCCGCGGGGCGGTCTCGGGGGCCGGGGATGCAGGGCAC
Chimp      ................................................................................
Gorilla    ................................................................................
Orangutan  ..........A...................................................................G.
Rhesus     ...................................C.....................T..A..T.....C........G.
Baboon     ...................................C...A....................A..T.....C........G.
Marmoset   ....................G.........C....C......T........T.........AC..T...C.....A..G.
Tarsier    .A....C.....A..............G.AC....CT..C.....C....CT..C..............C........G.
Lemur      ....................G.........G....C..........T......C.G.............C........G.
Mouse      AA.A.......C..T.A...GA........G....C.---------------..GT.T......A....C........T.
Cow        G..C........CC......G.C....G.AC....C...C.............C...............C........G.

Human      TGTCCTCCGGCAGTGGCAGCGAGTACAAGACCCACTTCGCAGCCTCGGTGACCGACCCCGAGAGGTTCTGGGGCAAAGCT
Chimp      ...........................................................................C....
Gorilla    ................................................................................
Orangutan  ...........................................G..............G.....................
Rhesus     ......................................A.........................................
Baboon     ......................................A.........................................
Marmoset   .......G................................C...........G...........................
Tarsier    .......A.....C...G.........G......................GG............................
Lemur      ..G....G.....C.TGG.............G........G......C..G.............................
Mouse      ..A..A..........TG.T..........................T...G.T........A..A........T......
Cow        .......G...---...G......................G......................................C

Human      GCCGAGCAGATCAGCTGGTACAAGCCCTGGACCAAAACGCTGGAGAACAAACACTCGCCCTCTACCAGgt
Chimp      ....................................................................t.
Gorilla    ...........................................................T........t.
Orangutan  ....................................................................t.
Rhesus     ..A.................................................................t.
Baboon     ..T.................................................................t.
Marmoset   ..T...........................................................C.....t.
Tarsier    ..G..............................................G....C....T.TC.....t.
Lemur      .........................................A.......G....C.T..T..C.....t.
Mouse      ..T...........T.......................T..A....G..G.T..C....T..C..A..t.
Cow        ..G................................G.............G...T.....T..C.....t.

26. uc001wxi.1_1_3

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
26	uc001wxi.1_1_3	chr14:50472312	-	GT	CT	(D7) exonization; novel start	constitutive	C14orf182	NP_001012724.1	NM_001012706.1	chromosome 14 open reading frame 182	de novo protein

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr14	50472312	50472517	-	GT	ATGACAGGTCGGATGGCAACCCTGGAAAAGTCACACAGCTCAGCCTGTTGGAGGAAGAGGAGCAGTAGAACCTGTGTGGAGCCTGACAGAACCCAAGATGCCATTCACGAACCGAGAGGTCTTTCAAGATCACACACAGTTCTACGACACAGACATTTCGTCTTCTTGCCACTCAGTAGCGGGGCCCATCCCTCTGTGCCTCCAAG
Chimp	panTro2	chr14	49196418	49196623	-	CT	ATAACAGGTCGGATGGCAACCCTGGAAAAGTCACACAGCTCAGCCTGTTGGAGGAAGAGGAGCAGTAGAACCTGTGTGGAGCCTGACAGAACCCAAGATGCCATTCACGAACCGCGAGGTCTTTCAAGATCACACACAGTTCTACGACACAGACATTTCATCTTCTTGCTACTCAGTAGCGGGGCCCATCCCTCTGTGCCTCCAAG
Orangutan	ponAbe2	chr14	50438720	50438925	-	CT	ATGACAGGTCGGATGGCAACCCTGGAAAAGTCACACAGCTCAGCCTGTTGGAGGAAGAGGAGCAGTAGAACCTGTGTGGAGCCTGACAGAACCCAAGATGCCATTCACGAACCGAGAGGTCTTTCAAGATCACACACAGTTCTATGACACAGACATTTCGTCTTCTTGCCACTCAGTAGCGGGGCCCGTCCCTCTGTGCCTCCAAG
Rhesus	rheMac2	chr7	113073642	113073847	-	CT	GTGACAGGTTGGATGGCAACCCTGGAAAAGCCACACACCTTGGCCTGTTGGAGGAAGAGGAGCAGTAGAACCTGCGTGGAGCCTGACAGAACCCAAGATGCCGTTCAGGAACCGAGAGGCCTTCCAAGATCACACACAGTTCTACAACACAGACATTTCGTCTTCTTGCCACTTAGCAGAGGGGCCCATCCCTCTGTGCCTCCAAG
Baboon	papHam1	scaffold19136	24672	24877	-	CT	GTGACAGGTTGGATGGCAACCCTGGAAAAGCCACACACCTTGGCCTGTTGGAGGAAGAGGAGCAGTAGAACCTGCGTGGAGCCTGACAGAACCCAAGATGCCGTTCAGGAACCGAGAGGCCTTTCAAGATCACACACAGTTCTACAACACAGACATTTCGTCTTCTTGCCACTTAGCAGCGGGGCCCATCCCTCTGTGCCTCCAAG
Lemur	micMur1	scaffold_2039	125594	125769	-	CT	ATGATGGGTCTGATGGCAACCCAGGAAAAGCCACACAGCTCAGCCTATTGGAGAAAGAGGAGCAACAGAACCCGTGTGGAGCCCGACAGAGCCCAGCACATAGCTCAGGAACCAAGAGGTCCTTCAAGATCAAACCCAGCTCACCAGCAGCAGGGACCATCCATTGCGCCTCCCAG
Galago	otoGar1	scaffold_115424.1-163071	721	892	-	CT	AAGATGTGTCCGATGGCAACCCAGGAAAAGTCCCGAGGCTCGGCCTGGTGGAGGAAGAGCAGCAGAACCTGGGTGGAGGCCGATAGTATCCAGGACCATGTCTCAGGAACCAAGAGGTCTTTCCAGATTAAACACATCTTACCAGCAGCAGGGATGGGCCCTTGTCCCCATG

Alignment (splice site sequences are in lowercase)

Human      ATGACAGGTCGGATGGCAACCCTGGAAAAGTCACACAGCTCAGCCTGTTGGAGGAAGAGGAGCAGTAGAACCTGTGTGGA
Chimp      ..A.............................................................................
Orangutan  ................................................................................
Rhesus     G........T....................C......C..TG................................C.....
Baboon     G........T....................C......C..TG................................C.....
Lemur      ....TG....T...........A.......C...............A......A..........AC......C.......
Galago     .A..TGT...C...........A.........C.GAG....G.....G........---......C........G.....

Human      GCCTGACAGAACCCAAGA-TGCCATTCACGAACCGAGAGGTCTTTCAAGATCACACACAGTTCTACGACACAGACATTTC
Chimp      ..................-................C............................................
Orangutan  ..................-..............................................T..............
Rhesus     ..................-....G....G...........C...C.....................A.............
Baboon     ..................-....G....G...........C.........................A.............
Lemur      ...C......G....GC.-CATAGC...G.....A.......C..........A..C...C..-----------------
Galago     .G.C..T..T.T...G..CCATGTC...G.....A...........C....T.A.....TC.T-----------------

Human      GTCTTCTTGCCACTCAGTAGCGGGGCCCATCCCTCTGTGCCTCCAAGgt
Chimp      A.........T....................................c.
Orangutan  ............................G..................c.
Rhesus     ..............T..C..A..........................c.
Baboon     ..............T..C.............................c.
Lemur      ------------AC...C...A...A......A.-..C......C..c.
Galago     ------------AC...C...A...ATGGG....---..T.C...T.c.

27. uc010tyr.1_1_8

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
27	uc010tyr.1_1_8	chr14:105939849	+	GT	GC	(D7) exonization; novel start	alternative	CRIP2	B7Z6C0	AK300092.1	cysteine-rich protein 2

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr14	105939585	105939849	+	GT	ATGCCTCCTCACCATCTTCTGCCTTGGCTGGCACAGGTCCCAAGTGCAGAGGGGGAGCTGGTCAGGCTGGTCAGTCGGGCGGGGGGGCGGGGGGCATGTTTCTGGCCTGCTGTCACCATGGAAATGGCAGTGGCTGCAGGGTGTGTGTGCAAGGGAGGAGGGTGCTGCCACAGAGAGCCCAGCCAGGACCACCATGAAAGCCAGGAGCACCGAGGGCCTCTGGTTGGCTCACAAACATGCCTGGTGCACCAGGCAGAGGGAACAG
Chimp	panTro2	chr14	106009367	106009631	+	GC	ATGCCTCCTTGCCATCTTCTGCCTTGGCTGGCACAGGTCCCAAGTGCAGAGGGGGAGCTGGTCAGGCTGGTCAGTTGGGCGGGGGGGCGGGGGGCATGTTTCTGGCCTGCTGTCACCATGGAAATGGCAGTGGCTGCAGGGTGTGTGTGCAAGGGAGGAGGGTGCTGCCACAGAGAGCCCAGCCAGGACCACCATGAAAGCCAGGAGCACCGAGGGCCTCTGGTTGGCTCACAAACATGCCTGGTGCACCAGGCAGAGGGAACAG
Orangutan	ponAbe2	chr14	107255556	107255820	+	GC	ATGCCTCCTCGCCATCTTCTGCCTTGGCTGGCACAGGTCCCAAGTGCAGAGGGGGAGCTGGTCAGGCTGGTCAGTCGGGCGTGGGGGCGGGGGGCATGTTTCTGGCCTGCTGTTACCATGGAAATGGCAGTGGCTGCAGGGTGTGTGTGCAAGGGAGGAGGGTGCTGCCATAGAGAGCCCGGCCAGGGCCACCATGAAAGCCAGGAGCACCGAGGGCCACTGGTTGGCTCACAAACATGCCTGGTGCACCAGGCAGAGGAAACAG
Rhesus	rheMac2	chr7	168836280	168836540	+	GC	ATGCCTCCTCGCCATCTTCTGCCTTGGCTGGCACAGATCCCAAGTGCAGAGGGGGAGCTGGTCAGGCTGGTCAGTCCGGCGTGGGGGCGGGGGGCATGTTTCTGGCCTGCGGTCACCATGGAAATGGCAGTGGCTGCAGGGTGTGTGTGCAAGGGAGGAGGGTGCTGCCACAGGGAGCCCTGCCAGGGCCACCATGAAAGCCAGGAGTGCCTAGGGCCACTGGCTCACAGACATGCCTGGTGCACCAGGCAGAGGAAACAG
Baboon	papHam1	scaffold9107	50694	50954	+	GC	ATGTCTCCTCGCCATCTTCTGCCTTGGCTGGCACAGATCCCAAGTGCAGAGGGGGAGCTGATCAGGCTGGTCAGTCCGGTGTGGGGGCGGGGGGCATGTTTCTGGCCTGCTGTCACCATGGAAATGGCAGTGGCTGCAGGGTGCGTATGCAAGGGAGGAGGGTGCTGCCACAGGGAGCCCTGCCAGGGCCACCATGAAAGCCAGGAGTGCCTAGGGCCACTGGCTCACAGACATGCCTGGTGCACCAGGCAGAGGAAACAG

Alignment (splice site sequences are in lowercase)

Human      ATGCCTCCTCACCATCTTCTGCCTTGGCTGGCACAGGTCCCAAGTGCAGAGGGGGAGCTGGTCAGGCTGGTCAGTCGGGC
Chimp      .........TG................................................................T....
Orangutan  ..........G.....................................................................
Rhesus     ..........G.........................A.......................................C...
Baboon     ...T......G.........................A.......................A...............C..T

Human      GGGGGGGCGGGGGGCATGTTTCTGGCCTGCTGTCACCATGGAAATGGCAGTGGCTGCAGGGTGTGTGTGCAAGGGAGGAG
Chimp      ................................................................................
Orangutan  .T...............................T..............................................
Rhesus     .T............................G.................................................
Baboon     .T.............................................................C..A.............

Human      GGTGCTGCCACAGAGAGCCCAGCCAGGACCACCATGAAAGCCAGGAGCACCGAGGGCCTCTGGTTGGCTCACAAACATGC
Chimp      ................................................................................
Orangutan  ..........T.........G......G..............................A.....................
Rhesus     .............G......T......G...................TG..T......A.----.........G......
Baboon     .............G......T......G...................TG..T......A.----.........G......

Human      CTGGTGCACCAGGCAGAGGGAACAGgt
Chimp      ..........................c
Orangutan  ...................A......c
Rhesus     ...................A......c
Baboon     ...................A......c

28. uc010ujw.1_1_8

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
28	uc010ujw.1_1_8	chr15:67841545	+	GT	TT	(D7) exonization; novel start	alternative	MAP2K5	NP_001193733.1	NM_001206804.1	mitogen-activated protein kinase kinase 5

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr15	67841519	67841545	+	GT	ATGATGGAGGGTCACTTTCCCCAGAGC
Chimp	panTro2	chr15	65257274	65257300	+	TT	ATGATGGAGGGTCACTTTCCCCAGAGC
Gorilla	gorGor1	Supercontig_0000754	44665	44691	+	TT	ATGATGGAGGGTCACTTTCCCCAGAGC
Orangutan	ponAbe2	chr15	64708352	64708378	+	TT	ATGATGGAGGGTCACTTTCCCCAGAGC
Rhesus	rheMac2	chr7	46140046	46140072	+	TT	GTGATGGAGGGTCACTTTTCCCAGAGT
Baboon	papHam1	scaffold1574	131910	131936	-	TT	GTGATGGAGGGTCACTTTCCCCAGAGT
Marmoset	calJac1	Contig3372	128771	128797	-	TT	ATGATGGACGGTCACTTTCCCCAGAGC
Galago	otoGar1	scaffold_105979.1-28627	10120	10146	+	TG	ATAGTGGAGGGTCACTTTCCCCAGAAC
Cow	bosTau4	chr10	14258568	14258594	+	TG	ATCATGGAGGGTCACTTTTCCCAGAGC
Dog	canFam2	chr30	34664024	34664050	+	TG	ATAATGGAGGGTCACTTTTCCCAGAAC

Alignment (splice site sequences are in lowercase)

Human      ATGATGGAGGGTCACTTTCCCCAGAGCgt
Chimp      ...........................t.
Gorilla    ...........................t.
Orangutan  ...........................t.
Rhesus     G.................T.......Tt.
Baboon     G.........................Tt.
Marmoset   ........C..................t.
Galago     ..AG.....................A.tg
Cow        ..C...............T........tg
Dog        ..A...............T......A.tg

29. uc002ayt.1_10_14

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
29	uc002ayt.1_10_14	chr15:75114241	+	GT	GC	(D5) exonization; inframe	constitutive	LMAN1L	NP_068591.2	NM_021819.2	lectin, mannose-binding, 1 like

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr15	75114170	75114241	+	GT	GCCCTGGATGCTTCCTGCCAGATTCCATCCACCCCAGGGAGGGGTGGCCACCTCTCCATGTCACTCAATAAG
Chimp	panTro2	chr15	72654849	72654920	+	GC	GCCCTGGATGCTTCCTGCCAGATTCCATCCACCCCAGGGAGGGGTGGCCACCTCTCCATGTCACTCAATAAG
Gorilla	gorGor1	Supercontig_0040171	29450	29521	-	GC	GCCCTGGATGCTTCCTGCCAGATTCCATCCACCCCAGGGAGGGGTGGCCACCTCTCCATGTCACTCAATAAG
Orangutan	ponAbe2	chr15	72232961	72233032	+	GC	GCCTTGGACTCTTCCTGCCAGATTCCATCCACCCCAGGGAGGGGTGGCCACCTCTCCATGTCACTCAATAAG
Lemur	micMur1	scaffold_3915	77454	77525	-	GC	GCCCTGCACCCCTCCTGCCAGGTTCCATCAGCCCCAGAGAAACGTGGCCACCTCTCCAGGTCACTCAGCGAG
Mouse	mm9	chr9	57458203	57458274	-	AC	GCCCTGGACCCCTCTTGCTTGATTGCACTCACCCTCAGAAATGTCAGCTACCTCTCCAGGTCCTTCTGCCAG
Cow	bosTau4	chr21	33930813	33930884	-	AT	GCCCAGGACTCCCCCTGCCAGGCTCCACCCACCCCTGAGAGAGGTGGCTCCCTCTCCAGGTCACTGGGCAAG
Dog	canFam2	chr30	40881753	40881824	+	AC	GCCCAGAACTCCTCCTGCCAGGTTCTACCCACCCCGGAGAGGGGCGGTCACTTCTCCAGGCCTCTCAGCGAG

Alignment (splice site sequences are in lowercase)

Human      agGCCCTGGATGCTTCCTGCCAGATTCCATCCACCCCAGGGAGGGGTGGCCACCTCTCCATGTCACTCAATAAGgt
Chimp      ...........................................................................c
Gorilla    ...........................................................................c
Orangutan  .....T....CT...............................................................c
Lemur      ........C.CC.C.........G.......AG......A..AAC...............G........GCG...c
Mouse      ..........CC.C..T...TT....G..CT.....TCA.A.AT.TCA..T.........G...CT..TGCC..ac
Cow        ......A...CT.CC........GC....C.......T.A...A......TC........G......GGGC...a.
Dog        ......A.A.CT.C.........G...T.C.......G.A......C..T...T......G.C.T....GCG..ac

30. uc002fck.2_4_9

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
30	uc002fck.2_4_9	chr16:72863678	-	GT	GC	(D3) shift; decrease; inframe	constitutive	ZFHX3	NP_001158238.1	NM_001164766.1	zinc finger homeobox protein 3

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr16	72863678	72863758	-	GT	AAGAAGCCATTGAAGATGTTGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGACCAAGAGGGCGGAG
Chimp	panTro2	chr16	72709211	72709291	-	GC	AAGAAGCCATTGAAGATGTTGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGACCAAGAGGGCGGAG
Gorilla	gorGor1	Supercontig_0462669	312	392	+	GC	AAGAAGCCATTGAAGATGTGGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGACCAAGAGGGCGGAG
Orangutan	ponAbe2	chr16	58950074	58950154	+	GC	AAGAAGCCATTGAAGATGTTGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGACCAAGAGGGCGGAG
Rhesus	rheMac2	chr20	69825299	69825379	+	GC	AAGAAGCCATTGAAGATGTTGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGAGCAAGAGGGTGGAG
Baboon	papHam1	scaffold12248	39346	39426	-	GC	AAGAAGCCATTGAAGATGTTGAAGGACCCAATGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGAGCAAGAGGGTGGAG
Marmoset	calJac1	Contig2164	66443	66523	+	GC	AAGAAGCCATTGAAGATGTTGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGACCAAGAGAGTGGAG
Tarsier	tarSyr1	scaffold_1587	56912	56992	-	GC	AAGAAGCCATTGAAGATGTTGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTCGCCAAGGACCAAGAGAGCGGAG
Galago	otoGar1	scaffold_112367.1-262559	204075	204155	-	GC	AAGAGGCTGTTGAAGATGTTGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGACCAAGAGAGTGGAG
Mouse	mm9	chr8	111438422	111438502	+	GC	AGGAAGCCGTTGAAGATGCCGAAGGACCCAGCGAAGCATCTGCTGACCCAGAGGAGCTGGCCAAAGACCAGGGGAGCGGCA
Cow	bosTau4	chr18	37377548	37377628	+	GC	AAGAAGCCATTGAAGATGTTGAAGGGCCCAGTGAAGCGGCTGCTGATCCCGAGGAGCTCGCTAAAGACCAGGAGAGTGGAG
Dog	canFam2	chr5	81503471	81503551	-	GC	AAGAAGCCATTGAAGATGTTGAAGGGCCCAGTGAAGCGGCTGCTGATCCAGAGGAGCTTGCTAAAGACCAGGAGAGTGGAG

Alignment (splice site sequences are in lowercase)

Human      agAAGAAGCCATTGAAGATGTTGAAGGACCCAGTGAAACAGCTGCTGATCCAGAGGAGCTTGCTAAGGACCAAGAGGGCG
Chimp      ................................................................................
Gorilla    .....................G..........................................................
Orangutan  ................................................................................
Rhesus     .....................................................................G........T.
Baboon     ................................A....................................G........T.
Marmoset   ............................................................................A.T.
Tarsier    ............................................................C..C............A...
Galago     ......G..TG.................................................................A.T.
Mouse      ...G......G.........CC...........C...G..T.......C...........G..C..A.....G.G.A...
Cow        ...........................G.........G.G...........C........C.....A.....G...A.T.
Dog        ...........................G.........G.G..........................A.....G...A.T.

Human      GAGgt
Chimp      ....c
Gorilla    ....c
Orangutan  ....c
Rhesus     ....c
Baboon     ....c
Marmoset   ....c
Tarsier    ....c
Galago     ....c
Mouse      .CA.c
Cow        ....c
Dog        ....c

31. uc010vqa.1_2_3

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
31	uc010vqa.1_2_3	chr17:650785	-	GT	AT	(D9) intronization; frameshift	alternative	GEMIN4	B4DZ29	AK302722.1	gem (nuclear organelle) associated protein 4

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr17	650785	651272	-	GT	GACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGCAGCTGTTCCACCCTAAGGCACTGGCAGAATTAACAAAGTCTGACTGGGAACGTGTTGGACGGCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTGATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTGACCCCGTCCGACACAGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACACCATCCTCTTCGAGCTGCTCAAATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCTGCCATGCAGAACTAGAGCGCTTTCTGGAACATGTGACCGTTGACACTTCTGCCGAAGACGTGGCCTTCTTCCTGGACGTCTGGTGGGAG
Chimp	panTro2	chr17	669117	669604	-	AT	GACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCTGAGCAGCTGTTCCACCCTAAGGCACTGGCAGAATTAACAAAGTCTGACTGGGAACATGTTGGACGGCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTGATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTGACCCCGTCCGACACAGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACACCATCCTTTTCGAGCTGCTCAAATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCTGCCATGCAGAACTAGAGCGCTTTCTGGAACACGTGACCGTTGACACTTCTTCCGAAGACGTGGCCTTCTTCCTGGACGTCTGGTGGGAG
Gorilla	gorGor1	Supercontig_0428564	107	594	-	AT	GACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCTGAGCAGCTGTTCCACCCTAAGGCACTGGCAGAATTAACAAAGTCTGACTGGGAACATGTTGGACGGCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTGATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCTGTGACCCCGTCCGACACAGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACACTGTCCTCTTCGAGCTGCTCAAATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCTGCCATGCAGAACTGGAGCGCTTTCTGGAACACGTGACCGTTGACACTTCTTCTGAAGACGTGGCCTTCTTCCTGGACGTCTGGTGGGAG
Orangutan	ponAbe2	chr17	571451	571938	-	AT	GACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGCAGCTGTTCCGCCCTAAGGCCCTGGCAGAATTAACAAAGTCTGACTGGGAACATGTTGGACGGCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTGATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTGACCCCGTCTGACACGGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCATACCGTCCTCTTTGAGCTGCTCAAATCCCTGGAAGCTTCTGCACTCTTTATCCAGCTCCTGATGGCCCTGCCCACTACCATCTGCCATGCAGAACTAGAGCGCTTTCTGGAACACGTGACCGTTGACACTTCTTCTGAAGATGTGGCCTTCTTCTTGGACGTCTGGTGGGAG
Rhesus	rheMac2	chr16	539100	539587	-	AT	GGCCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGCAGCTGTTCCGCCCCAAGGCACTGGCAGAATTGACAAAGTCTGACTGGGAACGTGTTGGACGCCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCCGCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTGATTATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTGACCCCCTCCGACATGGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACACTGTCCTTTTTGAGCTGCTCAAATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCCGCCATGCAGAACTAGAGCGCTTTCTGGAGCACGTGACCGTTGACACTTCTTCTGAAGACGTGGCCTTCTTCCTGGACGTCTGGTGGGAG
Baboon	papHam1	scaffold16075	30178	30665	+	AT	GGCCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGCAGCTGTTCCGCCCCAAGGCACTGGCAGAATTGACAAAGTCTGACTGGGAACATGTTGGACGCCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCCGCGGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTGATTATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTGACCCCCTCCGACACGGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACACTGTCCTCTTTGAGCTGCTCAAATCCCTGGAAGCTTCTGGACTCTTTATCCAACTCCTGATGGCCCTGCCCACCACCATCCGCCATGCAGAACTAGAGCGCTTTCTGGAGCACGTGACTGTTGACACTTCTTCTGAAGATGTGGCCTTCTTCCTGGACGTCTGGTGGGAG
Marmoset	calJac1	Contig1580	411261	411748	+	AT	GACCCTTGAACATCTGTGAGGAAATGACTATTCTGCACGGAGGCTTCCTGTTGGCTGAGCAGCTGTTCCACCCCAGAGCTCTGACAGAACTGACCAAGTCTGACTGGGAGCACGTTGGGCGGCCCATTGTGGAGGCCTTGCAGGAGATCTCCTCCGCAGCAGCGCATTCCCAGCCCTTTGCCTGGAAGAAGAAAGCTCTGATCATCATCTGGGCCAAGGTTCTGCAGCCTCAGCCTGTGACCTCTTCTGACACGGACACTCGGTGGCAGGAAGATGTGTTCTTCTCAGTGGGCAATATGATCCCCACCCTCAATCACACCGTCCTGTTTGAGCTGCTCAAGTCCCTAGAAGCTTCTGGACTCTTTATCGAGCTCCTGATGGCCCTGCCTGCCATTGTCTGCTGTGCAGAACTCGAGCGCTTTTTGGAGCACGTGAGCATTGACACGTCCTCTGAGGACGTGGCCTTCTTCCTGGATGTCTGGTGGGAA
Tarsier	tarSyr1	scaffold_10072	26491	26978	+	AT	GGGCCTTGAACATCTGTGAAGAAATAACTATCCTGCATGGGGGCTTCTTGCTGGCCGAGCAGCTGTTCCACCCCAAAGTATTGGCAGACTTGGCAAAGTCTGACTGGGAACATGTTGGGAGGCCCATTGTGGAGGCCTTGAGGGAGATCTCCTCAGCCACTGCACATTCCCAGCCCTTTGCCTGGAAGAAGAAAGCTCTGATCATCATCTGGGCCAAGGTTCTTCAGCCCTGCCCTGTTAGCCCTTCTGACACTGAAACTCGGTGGCAGGAAGATGTGTTCTTCTCAGTGGGCAACATGATCCCTACTATCAATCACACAGTTCTTTTTGAGCTGCTCAAATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCTACCACGGTCTGCCGCACAGAACTAGAGCGCTTTTTGGAGCACATGACTACTGACACTTCATCCAAAGATGTGGCCTTCTTCCTCGATGTCTGGTGGGAG
Lemur	micMur1	scaffold_1499	49020	49507	-	AT	GACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGGGGCTTCTTGCTGGCCGAGCAGCTGTTCCGCCCCAAAGCACTGGCAGAATTGACAAAGTCTGACTGGGAGCACGTTGGGCGGCCCATCGTGGAGGCCTTGAGGGAGATCTCCTCGGCCACAGCGCATGCACAGTCCTTTGCCTGGAAGAAGAAAGCTCTGATCATCATCTGGGCCAAGGTTCTTCAACCCTGCCCCGTTACCCCTTCTGACACTGAAGCTCGGTGGCAGGAAGATGTGTTCTTCTCAGTAGGCAACATGATCCCTACAATCAACCACACAGTCCTTTTTGAGCTGCTCAAGTCCCTGGAAGCTTCTGGACTCTTTATTCAGCTCCTGATGGCTCTGCCCACCACCATCTGCCGTGCAGAACTAGAGCGCTTTTTGGAGCACATGACTATTGACACTTCTTCCAAGGACGTGGCCTTCTTCCTGGATGTCTGGTGGGAA
Galago	otoGar1	scaffold_16583.1-122997	45211	45698	+	AT	GACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGGGGCTTCTTGCTGGCTGAGCAGCTGTTCCACCCCAGAGCATTGGCAGAATTGACAAAGTCTGACTGGGAACATGTTGGCCGGCCCATTGTGGAAGCCTTACGGGAGATCTCCTCAGCCACAGCACACTCCCAATCCTTTGCCTGGAAGAAGAAAGCTCTCATCATTATCTGGGCCAAAGTTCTTCAGCCCTACCCTGTTACCCCTTCTGACACTGAAACTCGGTGGCAGGAAGATGTATTTTTCTCGGTAGGTAACATGATCCCTACCATCAATCACACAGTCCTTTTTGAGCTGCTCAAGTCCCTGGAAGCTTCTGGACTTTTTATTCAGCTCCTCATGGCTCTGCCCACCACCATCTGCCGCACAGAACTTGAGCGCTTTTTGGAGCACATGACTATTGACACTTCTTCTAGGGATGTGGCCTTCTTCTTGGATGTCTGGTGGGAA
Mouse	mm9	chr11	76026938	76027425	-	AT	GACCCTTGAATATCTGTGAGGAGATGACTATTCTGCATGGGGGCTTCTTGATGGCTGAGCAGCTGTTCCACCCCAAAGCCCTGGTACAGTTGACCAAGTCTGATTGGGAACACGTGGGACAGCCCATTGTGGAGGCCTTGAAGGAGATCTCCTCAGCCACAGCACATTCCCAGCCCTTTGCCTGGAAGAAGAAAGCACTGATCATCATCTGGACCAAGGTTCTTCAGCCCTCCCCTGTCAGCCCTTCTGACACTGATACTCGGTGGCAGGAAGATGTATTCTTCTCCGTGGGCAACATGATCCCTACCATCAACCACACAGTCCTTTTTGAGCTGCTCAAGTCTCTGGAAGCTTCTGGACTCTTCATTCAGCTGCTAATGGCCTTGCCTACCACCATTGGCCGCTCAGAACTACAGAGCTTCCTGGAGCACATGACTGTTGACACATCTTCCAAGGATGTGGCCTTCTTCTTAGATGTCTGGTGGGAA
Cow	bosTau4	chr19	22114444	22114931	+	AT	GACCTTTGAATATCTGTGAGGAAATGACTATCTTGCACGGGGGCTTCTTGCTGGCTGAGCAGCTGTTCCGCCCCAAAGCGCTGGCCGAACTGACCAAGTCTGACTGGGTGCACGTTGGGCGGCCCATTGTGGAGGCTCTGAGGGAGATCTCCTCCACCACAGCCTGCTCCCAGCCCTTCGCCTGGAAGAAGAAAGCGCTGATCATCATCTGGGCCAAGGTGCTACAGCCCTACCCCATCACCCCTTCCGACATGGAAACCCGCTGGCAGGAAGATGTGTTCTTCTCTGTGGGCAACATGATCCCCACCATCAATCACACAGTCCTCTTTGAGCTGCTCAAGTCGCTGGAGGCCTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACTGTCTGCCGTGCGGAACTAGAGCACTTTTTGGAGCACATGACCATCGACACCTCTTCCAAGGACGTGGCCTTCTTCCTCGACGTCTGGTGGGAA
Dog	canFam2	chr9	48367803	48368290	+	AT	GACCTTTGAATATCTGTGAAGAAATGACTATTCTGCATGGGGGCTTTTTGCTTGCCGAGCAGCTCTTCCACCCCAAAGCACTGACAGAACTGACAAAGTCTGACTGGGAACATGTTGGGCAGCCCATTGTGGAGGCCCTAAGGGAGATCTCCTCCGCCACAACATGCTCCCAGCCCTTTGCCTGGAAGAAGAAAGCTCTGATCATCATCTGGGCCAAGGTTCTTCAGCCCTACCCTGTCACCCCTTCAGACACTGACACTCGGTGGCAGGAAGATGTGTTCTTCTCGGTAGGCAACATGATCCCTACCATCAATCACACGGTCCTTTTTGAGCTGCTCAAGTCTCTGGAAGCTTCTGGACTCTTTATCCAGCTTCTGATGGCCCTGCCCACCACCATCTGCCATGCAGAACTAGAGCGCTTTTTGGAGCACATGTCTGTTGACACTTCTTCAGAGGATGTGGCCTTTTTCCTCAGTGTCTGGTGGGAA

Alignment (splice site sequences are in lowercase)

Human      agGACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGCAGCTGTTCCACCCTAAGG
Chimp      .........................................................T......................
Gorilla    .........................................................T......................
Orangutan  .......................................................................G........
Rhesus     ...G...................................................................G...C....
Baboon     ...G...................................................................G...C....
Marmoset   .....................G.................C.........C..T....T.................C.GA.
Tarsier    ...GG......................A.....C........G................................C..A.
Lemur      ..........................................G............................G...C..A.
Galago     ..........................................G..............T.................C.GA.
Mouse      ............T........G..G.................G.........A....T.................C..A.
Cow        ......T.....T........G...........CT....C..G..............T.............G...C..A.
Dog        ......T.....T.............................G.....T.....T...........C........C..A.

Human      CACTGGCAGAATTAACAAAGTCTGACTGGGAACGTGTTGGACGGCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCT
Chimp      .................................A..............................................
Gorilla    .................................A..............................................
Orangutan  .C...............................A..............................................
Rhesus     .............G.............................C...................................C
Baboon     .............G...................A.........C...................................C
Marmoset   .T...A.....C.G..C..............G.AC.....G........T...........GCA............C..A
Tarsier    T.T.......C..GG..................A......GA.......T...........G..............A..C
Lemur      .............G.................G.AC.....G....................G.................C
Galago     ..T..........G...................A......C........T.....A......C.............A..C
Mouse      .C....T.C.G..G..C........T.......AC..G....A......T...........G.A............A..C
Cow        .G.....C...C.G..C.............TG.AC.....G........T........TC.G..............CA.C
Dog        .....A.....C.G...................A......G.A......T.........C................C..C

Human      GCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTGATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGT
Chimp      ................................................................................
Gorilla    .............................................................................T..
Orangutan  ................................................................................
Rhesus     ............................................T...................................
Baboon     ..G.........................................T...................................
Marmoset   .....G..T.............................T................................T..G..T..
Tarsier    A.T.....T.............................T..........................T.....CTG...T..
Lemur      A....G..TG.A...T......................T..........................T..A..CTG......
Galago     A.............AT......................T..C.....T...........A.....T.....CT....T..
Mouse      A.......T.............................A...............A..........T.....CTC...T..
Cow        A....CTG............C.................G.......................G..A.....CT.....A.
Dog        A..A..TG..............................T..........................T.....CT....T..

Human      GACCCCGTCCGACACAGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACA
Chimp      ................................................................................
Gorilla    ................................................................................
Orangutan  .........T.....G..............................................................T.
Rhesus     ......C.......TG................................................................
Baboon     ......C........G................................................................
Marmoset   ....T.T..T.....G..C..T..............TG..........A........T............C....T....
Tarsier    T.G...T..T.....T..A..T..............TG..........A.................T..T.....T....
Lemur      T.....T..T.....T..AG.T..............TG..........A..A..............T..A..........
Galago     T.....T..T.....T..A..T..............TG.A..T........A..T...........T........T....
Mouse      C.G...T..T.....T..T..T..............TG.A........C.................T.............
Cow        C.....T.......TG..A..C..C...........TG..........T..........................T....
Dog        C.....T..A.....T..C..T..............TG.............A..............T........T....

Human      CCATCCTCTTCGAGCTGCTCAAATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATC
Chimp      .......T........................................................................
Gorilla    .TG.............................................................................
Orangutan  ..G.......T............................C.................................T......
Rhesus     .TG....T..T.....................................................................
Baboon     .TG.......T.........................................A...........................
Marmoset   ..G....G..T...........G.....A.....................G...................TG...TTG..
Tarsier    .AG.T..T..T...........................................................T.....GG..
Lemur      .AG....T..T...........G..........................T..............T...............
Galago     .AG....T..T...........G....................T.....T........C.....T...............
Mouse      .AG....T..T...........G..T....................C..T.....G..A......T....T........T
Cow        .AG.......T...........G..G.....G..C.........................................TG..
Dog        .GG....T..T...........G..T.............................T........................

Human      TGCCATGCAGAACTAGAGCGCTTTCTGGAACATGTGACCGTTGACACTTCTGCCGAAGACGTGGCCTTCTTCCTGGACGT
Chimp      ................................C..................T............................
Gorilla    ..............G.................C..................T.T..........................
Orangutan  ................................C..................T.T.....T............T.......
Rhesus     C............................G..C..................T.T..........................
Baboon     C............................G..C.....T............T.T.....T....................
Marmoset   ...TG.........C.........T....G..C....G.A.......G..CT.T..G....................T..
Tarsier    ....GCA.................T....G..CA....TAC.........AT..A....T..............C..T..
Lemur      ....G...................T....G..CA....TA...........T..A.G....................T..
Galago     ....GCA.......T.........T....G..CA....TA...........T.TAGG..T............T....T..
Mouse      G...GCT........C..A....C.....G..CA....T........A...T..A.G..T............T.A..T..
Cow        ....G...G..........A....T....G..CA.....A.C.....C...T..A.G.................C.....
Dog        ........................T....G..CA..T.T............T.A..G..T........T.....CAGT..

Human      CTGGTGGGAGgt
Chimp      ..........a.
Gorilla    ..........a.
Orangutan  ..........a.
Rhesus     ..........a.
Baboon     ..........a.
Marmoset   .........Aa.
Tarsier    ..........a.
Lemur      .........Aa.
Galago     .........Aa.
Mouse      .........Aa.
Cow        .........Aa.
Dog        .........Aa.

32. uc002htp.2_2_5

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
32	uc002htp.2_2_5	chr17:38172107	+	GT	GC	(D1) shift; increase; inframe	alternative	CSF3	NP_000750.1	NM_000759.3	colony stimulating factor 3 (granulocyte)

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr17	38171944	38172107	+	GT	CCCTGCAGCTGCTGCTGTGGCACAGTGCACTCTGGACAGTGCAGGAAGCCACCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGAGCAAGTGAGGAAGATCCAGGGCGATGGCGCAGCGCTCCAGGAGAAGCTGGTGAGTGAG
Chimp	panTro2	chr17	17523558	17523721	-	GC	CCCTGCAGCTGCTGCTGTGGCACAGTGCACTCTGGACAGTGCAGGAAGCCACCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGAGCAAGTGAGGAAGATCCAGGGCGATGGCGCGGCGCTCCAGGAGAAGCTGGTGAGTGAG
Gorilla	gorGor1	Supercontig_0006833	10703	10866	-	GC	CCCTGCAGCTGCTGCTGTGGCACAGTGCACTCTGGACAGTGCAGGAAGCCACCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGAGCAAGTGAGGAAGATCCAGGGCGATGGCGCGGCACTCCAGGAGAAGCTGGTGAGTGAG
Orangutan	ponAbe2	chr17	49412086	49412249	-	GC	CCCTGCAGCTGCTGCTGTGGCACAGCGCACTCTGGACAGTGCAGGAAGCCACCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGAGCAAGTGAGGAAGATCCAGGGCGATGGCGCGGCACTCCAGGAGAAGCTGGTGAGTGAG
Rhesus	rheMac2	chr16	50114753	50114916	+	GC	CCCTGCAGCTGCTGCTGTGGCACAGCGCACTCTGGACAGTGCAGGAAGCCACCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGAGCAAGTAAGGAAGATCCAGGGCGACGGTGCCGCGCTGCAGGAGAAGTTGGTGAGTGAG
Baboon	papHam1	scaffold1311	36057	36220	-	GC	CCCTGCAGCTGCTGTTGTGGCACAGCGCACTCTGGACAGTGCAGGAAGCCACCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGAGCAAGTAAGGAAGATCCAGGGCGACGGTGCCGCGCTGCAGGAGAAGCTGGTGAGTGAG
Marmoset	calJac1	Contig9599	52140	52303	-	GC	CCCTGCAGCTGCTGCTATGGCACAGTGCACTCTGGACGGTGCAGGAAGCCGCCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGTTTCCTGCTCAAGTGCTTAGAGCAAGTGAGGAAGGTCCAGGGCGATGGCACGGAGTTGCAGGAGAAGCTGGTGAGTGAG
Lemur	micMur1	scaffold_3270	80075	80238	+	AG	CCCTGCAGCTGCTGCTCTGGCACAGCACACTCTGGCCAGCGCAAGAAGCCAGCCCCCTGGACCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTGGAGCAAGTGAGAAAGATCCAGAGCGACGGCGCAGCCCTGCAGGAGAAGCTGGTGAGTGGG
Galago	otoGar1	scaffold_20611.1-1041	376	539	-	GG	CCCTACAGCTGCTGCTCTGGCACAGTGCACTCTGGACGGTGCAGGAAGCCAGTCCCCTGGGCCCTGCCAGTTCCCTGCCCCAGGGCTTTCTGCTCAAGTGCTTAGAGCAAGTGAGGAAGATCCAGAATGAAGGTGCGGCCCTGCAGGAGAATCTGGTGAGTGAG
Mouse	mm9	chr11	98562818	98562999	+	GT	CCCTGCAGCTGCTGCTGTGGCAAAGTGCACTATGGTCAGGACGAGAGGCCGTTCCCCTGGTCACTGTCAGCGCTCTGCCACCATCCCTGCCTCTGCCCCGAAGCTTCCTGCTTAAGTCCCTGGAGCAAGTGAGGAAGATCCAGGCCAGCGGCTCGGTGCTGCTGGAGCAGTTGGTGAGTGGG
Cow	bosTau4	chr19	41596347	41596510	+	GG	TCCTGCAGCTGCTCCTCTGGCACAGTGCGCTCTGGACGGTGCACGAAGCCACCCCCCTTGGCCCTGCCCGATCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGAGCAAGTGAGGAAAATCCAGGCTGATGGCGCCGAGCTGCAGGAGAGGCTGGTGAGTGAG
Dog	canFam2	chr9	25859702	25859865	-	AG	CCCTGCAGCTGCTGCTGTGGCACAGCGCACTCTGGATGGTGCAAGAAGCCGCCCCCCTGGGCCCTACCGGCCCCCTGCCCCAGAGCTTCCTGCTCAAGTGCCTAGAGCAAATGAGGAAGGTCCAGGCTGATGGCACGGCGCTGCAGGAGACGCTGGTGAGGGAG

Alignment (splice site sequences are in lowercase)

Human      agCCCTGCAGCTGCTGCTGTGGCACAGTGCACTCTGGACAGTGCAGGAAGCCACCCCCCTGGGCCCTGCCAGC-------
Chimp      .........................................................................-------
Gorilla    .........................................................................-------
Orangutan  ...........................C.............................................-------
Rhesus     ...........................C.............................................-------
Baboon     ................T..........C.............................................-------
Marmoset   ..................A....................G............G....................-------
Lemur      ..................C........CA........C...C...A.......G........A..........-------
Galago     ......A...........C....................G.............GT.................T-------
Mouse      ........................A........A...T...GA.GA..G...GTT.......T.A...T....GCTCTGC
Cow        ..T............C..C...........G........G.....C..............T.........C.A-------
Dog        ...........................C..........TG.....A......G..............A..G..-------

Human      -----------TCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGAGCAAGTGAGGAAGATCCAGGGCGATGGCGCAGCG
Chimp      -----------.................................................................G...
Gorilla    -----------.................................................................G..A
Orangutan  -----------.................................................................G..A
Rhesus     -----------.........................................A.................C..T..C...
Baboon     -----------.........................................A.................C..T..C...
Marmoset   -----------..............T.................................G..............A.G.A.
Lemur      -----------................................G...........A.........A....C........C
Galago     -----------............G....T....................................AAT..A..T..G..C
Mouse      CACCATCCCTGC.T.......GA...........T....C.C.G......................C.AGC...T.G.T.
Cow        -----------...............................................A.......CT........C.A.
Dog        -----------C.............................C........A........G......CT......A.G...

Human      CTCCAGGAGAAGCTGGTGAGTGAGgt
Chimp      .........................c
Gorilla    .........................c
Orangutan  .........................c
Rhesus     ..G.........T............c
Baboon     ..G......................c
Marmoset   T.G......................c
Lemur      ..G...................G.ag
Galago     ..G........T.............g
Mouse      ..G.T....C..T.........G...
Cow        ..G.......G..............g
Dog        ..G.......C.........G...ag

33. uc002kms.1_1_6

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
33	uc002kms.1_1_6	chr18:5406764	-	GT	GC	(D1) shift; increase; inframe	alternative	EPB41L3	B3KT50	AK094952.1	erythrocyte membrane protein band 4.1-like 3

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr18	5406764	5406940	-	GT	ATGAAACATCAAACCAACATTAGCGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCGTAACGAATGAATGGGAGAAGAGGCTTTCCACCTCCCCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTCCCTGAAGAGGTCAGTAGTCAG
Chimp	panTro2	chr18	11263147	11263323	+	GC	ATGAAACATCAAACCAACATTAGCGAGCTGAAAAGAACCTTCTTAGAAACTTCAACAGACACTGCCGTAACGAATGAATGGGAGAAGAGGCTTTCCACCTCCCCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTCCCTGAAGAGGTCAGTAGTCAG
Gorilla	gorGor1	Supercontig_0010726	17793	17969	-	GC	ATGAAACATCAAACCAACATTAGCGAACTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCNTAACAAATGAATGGGAGAAGAGGCTTTCCACCTCCCCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTCCCTGAAGAGGTCAGTAGTTAG
Orangutan	ponAbe2	chr18	26952849	26953025	+	GC	ATGAAACATCAAACCAACATTAGCGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCATAACGAATGAATGGGAGAAGAGGCTTTCCACCTCCCCCGTACGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTCCCTGAAGAGGTCAGTAGTCAG
Rhesus	rheMac2	chr18	8880328	8880504	+	GC	ATGAAACATCAAACCAACATTAGCGAGCTAAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCATAACGAATGAATGGGAGAAGAGGCTTTCCACCTCCCCGGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTCCCTGAAGAGGTCAGTAGTCAG
Baboon	papHam1	scaffold9513	20770	20946	-	GC	ATGAAACATCAAACCAACATTAGCGAGCTAAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCATAACGAATGAATGGGAGAAGAGGCTTTCCACCTCCCCGGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTCCCTGAAGAGGTCAGTAGTCAG
Marmoset	calJac1	Contig2763	173492	173668	+	GC	ATGAAACATCAAACCAACATTAGTGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCTGTCACGAATGAATGGGAGAAGAGGCTTTCCACGTCCCCCGTGAGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTTCCTGAAGAGGTCAGTAGTCAG
Tarsier	tarSyr1	scaffold_192	25712	25888	-	GC	ATGAAACATCAAACCAACATTAGTGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCATAACGAATGAATGGGAAAAGAGGCTTTCTACCTCCCCAGTGCGACTAGCTGCCAGGCAGGAGGATGCCCCCATGATCGAGCCACTTGTCCCTGAAGAGGTCAGTAGTCAG
Galago	otoGar1	scaffold_114927.1-283004	223234	223410	-	GC	ATGAAACACCAGACTAACATCAGTGAGCTGAAAAGAACCTTCTTAGAAACTTCAACAGACACTGCCGTCACCAATGAGTGGGAGAAGAGGCTGTCCACCTCCCCGGTGCGGCTGGCAGCCAGGCAGGAGGACGCGCCCATGATCGAGCCGCTTGTGCCTGAGGAGGTCAGTGCACGG
Mouse	mm9	chr17	69624071	69624247	+	GC	ATGAAGCACCAAACCAATATTAGTGAGCTGAAAAGAACCTTCTTAGAAACCTCTACAGAAACTGCCTTAACAAACGAGTGGGAAAAGAGGCTCTCTACATCTCCCGTGCGGCTGGCGGCCAGGCAGGAGGATGCACCCATGATTGAGCCGCTGGTACCCGAGGAGGTCAGTATTCTG
Cow	bosTau4	chr24	40385828	40386004	-	GC	ATGAAACATCAGACCAATATTAGTGAGCTGAAAAGAACCTTTTTAGAAACCTCCACAGACACTGCCATCACGAACGAGTGGGAAAAGAGGCTTTCCACCTCCCCCGTGCGACTAGCCGCCCGGCAGGAGGATGCACCCATGATCGAGCCGCTGGTACCCGAAGAGGTTGGTATTCAG
Dog	canFam2	chr7	74830542	74830718	-	GC	ATGAAACACCAAACCAATATTAGTGAGCTGAAAAGAACCTTTTTAGAAACCTCCACAGACACTGCCATCACGAATGAATGGGAAAAGAGGCTTTCTACTTCCCCAGTGCGACTAGCCGCCAGGCAGGAGGACGCGCCCATGATCGAACCACTTGTACCTGAAGAGGTCGGTATTCGG

Alignment (splice site sequences are in lowercase)

Human      ATGAAACATCAAACCAACATTAGCGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCGTAACGAATGAATG
Chimp      ..................................................T.............................
Gorilla    ..........................A.......................................N....A........
Orangutan  ..................................................................A.............
Rhesus     .............................A....................................A.............
Baboon     .............................A....................................A.............
Marmoset   .......................T.........................................T..C...........
Tarsier    .......................T..........................................A.............
Galago     ........C..G..T.....C..T..........................T.................C..C.....G..
Mouse      .....G..C........T.....T.............................T.....A......T....A..C..G..
Cow        ...........G.....T.....T.................T...........C............A.C.....C..G..
Dog        ........C........T.....T.................T...........C............A.C...........

Human      GGAGAAGAGGCTTTCCACCTCCCCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTCCCTG
Chimp      ................................................................................
Gorilla    ................................................................................
Orangutan  ...........................A....................................................
Rhesus     ........................G.......................................................
Baboon     ........................G.......................................................
Marmoset   ..................G.........A..............................................T....
Tarsier    ...A...........T........A........A..T.............................G.............
Galago     ............G...........G.....G.....A..............C..G...........G..G.....G....
Mouse      ...A........C..T..A..T........G.....G.................A........T..G..G..G..A..C.
Cow        ...A.............................A......C.............A...........G..G..G..A..C.
Dog        ...A...........T..T.....A........A.................C..G....................A....

Human      AAGAGGTCAGTAGTCAGgt
Chimp      ..................c
Gorilla    ..............T...c
Orangutan  ..................c
Rhesus     ..................c
Baboon     ..................c
Marmoset   ..................c
Tarsier    ..................c
Galago     .G.........GCA.G..c
Mouse      .G..........T..T..c
Cow        .......TG...T.....c
Dog        ........G...T..G..c

34. uc010dpa.2_1_7

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
34	uc010dpa.2_1_7	chr18:48346267	-	GT	GG	(D7) exonization; novel start	alternative	MRO	NP_001120648.1	NM_001127176.1	maestro

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr18	48346267	48346285	-	GT	ATGGCGTGGGGAAGCAAAG
Chimp	panTro2	chr18	47083331	47083349	-	GG	ATGGCGTGGGGAAGCAAAG
Gorilla	gorGor1	Supercontig_0007196	18978	18996	-	GG	ATGGCGTAGGGAAGCAAAG
Orangutan	ponAbe2	chr18	63241274	63241292	-	GG	ATGTCGTGGGAAAGCAAAG
Rhesus	rheMac2	chr18	43915689	43915707	-	GG	ATGGCGTGGGGAAGCAAAG
Baboon	papHam1	scaffold5166	31203	31221	+	GG	ATGGCGTGGGGAAGCAAAG
Marmoset	calJac1	Contig56	634633	634651	+	GG	AAGGCGTGGGGAAGCCAAG
Lemur	micMur1	scaffold_9903	21889	21907	-	GG	ACAGCGAGGGCAAGCAAAG
Galago	otoGar1	scaffold_60966.1-8252	4401	4419	-	GG	ACAGTGAAGGGAAGCAAAG

Alignment (splice site sequences are in lowercase)

Human      ATGGCGTGGGGAAGCAAAGgt
Chimp      ....................g
Gorilla    .......A............g
Orangutan  ...T......A.........g
Rhesus     ....................g
Baboon     ....................g
Marmoset   .A.............C....g
Lemur      .CA...A...C.........g
Galago     .CA.T.AA............g

35. uc010zuc.1_1_15

Summary

No	Exon ID	Position (hg19)	Dir	Human donor	Chimp donor	Category	Usage	Gene symbol	Protein accession	mRNA accession	Gene title	Note
35	uc010zuc.1_1_15	chr20:31619550	+	GT	GC	(D1) shift; increase; inframe	alternative	BPIFB6	NP_777557.1	NM_174897.2	BPI fold containing family B, member 6	GTNGTN

Orthologs

Species	Assembly	Chromosome	Exon start	Exon end	Dir	Donor	Exon sequence
Human	hg19	chr20	31619454	31619550	+	GT	ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACTGGCACGCGAGCTGACCCTGGGGCACTGCTGCGGTTGGGCATGGACATCATGAACC
Chimp	panTro2	chr20	30013963	30014059	+	GC	ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACTGGCATGCGAGCTGACCCTGGGGCACTGCTGCGGTTGGGCATGGACATCATGAACC
Gorilla	gorGor1	Supercontig_0004547	33444	33540	-	GC	ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACTGGCACGCGAGCTGACCCTGGGGCACTGCTGCGGTTGGGCATGGACATCATGAACC
Orangutan	ponAbe2	chr20_random	5105759	5105855	+	GC	ATGCTGAGGATCCTGTGCCTGGCCCTCTGCAGCCTGCTGACTGGCACGCGAGCTGACCCTGGGGCACTGCTGAGGTTGGGCATGGACATCATGAACC
Rhesus	rheMac2	chr10	31516584	31516680	-	GC	ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACCCACGCGCGAGCTGACCCTGGGGCACTGCTGCGGTTGGGCATGGACATCATGAACC
Baboon	papHam1	scaffold5329	64718	64814	+	GC	ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACCCACGTGCGAGCTGACCCTGGGGCACTGCTGCGGTTGGGCATGGACATCATGAATC
Marmoset	calJac1	Contig2380	4972	5068	+	AC	ATGCTGCGGATCCTGGGCCTGGCACTCTGCAGCCTGCTGACCTGCACGCAAGCCGAGCCTGGGGCCCTGCTGCGGCTGGGCATGGACATCATGAACC
Lemur	micMur1	scaffold_1836	158643	158739	+	GA	ATGCTTCGGATCCTGTGTCTGGCACTCTGCAGCCTGCTGAGCGGCGCTCGAGCTGACCCGGGGGCGCTGCTGCGCTTGGGCATGGACGTCATGAACC
Galago	otoGar1	scaffold_100622.1-7485	2933	3029	+	GC	ATGCTGCGGATCCTATGTTTGGCACTCTGTGGCCTACTGACCGGCACACGAGCAGACCCTGGGGCGCTGCTGCGCTTGGGCATGGACATCATGAACC
Mouse	mm9	chr2	153728377	153728473	+	AT	ATGCTGTGTAGCCTGAGCCTGGTCCTCTGCGGCCTGCTGGCTGGCACTCGAGCTGACCCTGGGGGTCTTCTGAGGTTGGGCATGGACATTATGAACC
Cow	bosTau4	chr13	63203323	63203419	+	GC	ATGCTGTGGATCCTGTGTCTGGCGCTCTGCAGCCTGCTGACTCCCACGCGAGCAGACCCTGGGGCACTGCTGCGGCTGGGCATGGACGTCCTGAATC
Dog	canFam2	chr24	25272426	25272522	+	AC	ATGCTGTGGATCCTGTTTCTGGCACTCTGTGGCCTGCTGACCCACACCCGAGCTGATCCCGGGGCATTGCTGAGGCTGGGCATGGACGTCATGAACC

Alignment (splice site sequences are in lowercase)

Human      ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACTGGCACGCGAGCTGACCCTGGGGCACTGCTGCGGTTGGG
Chimp      ..............................................T.................................
Gorilla    ................................................................................
Orangutan  ......A................C................................................A.......
Rhesus     .........................................CCA.G..................................
Baboon     .........................................CCA.GT.................................
Marmoset   ...............G.........................CT......A...C..G........C.........C....
Lemur      .....T...........T......................GC...G.T...........G.....G........C.....
Galago     ..............A..TT..........TG....A.....C.....A.....A...........G........C.....
Mouse      ......T.T.G....A......TC......G........G.......T................GT..T...A.......
Cow        ......T..........T.....G..................CC.........A.....................C....
Dog        ......T.........TT...........TG..........CCA...C........T..C......T.....A..C....

Human      CATGGACATCATGAACCgt
Chimp      ..................c
Gorilla    ..................c
Orangutan  ..................c
Rhesus     ..................c
Baboon     ...............T..c
Marmoset   .................ac
Lemur      .......G..........a
Galago     ..................c
Mouse      .........T.......a.
Cow        .......G..C....T..c
Dog        .......G.........ac