From mailadmn@ebi.ac.uk Fri Nov 24 11:58:14 2000 Received: from alpha1.ebi.ac.uk [193.62.196.122] (root) by merry.microb.uni.wroc.pl with esmtp (Exim 2.05 #1 (Debian)) id 13zGYV-0008Nv-00; Fri, 24 Nov 2000 11:58:11 +0100 Received: from s11.sputnik.ebi.ac.uk (s11.sputnik.ebi.ac.uk [172.17.2.11]) by alpha1.ebi.ac.uk (8.8.8/8.8.7) with ESMTP id JAA14314 for ; Fri, 24 Nov 2000 09:58:18 GMT Received: (from mailadmn@localhost) by s11.sputnik.ebi.ac.uk (8.9.3/8.9.3) id JAA24648; Fri, 24 Nov 2000 09:58:18 GMT Date: Fri, 24 Nov 2000 09:58:18 GMT Message-Id: <200011240958.JAA24648@s11.sputnik.ebi.ac.uk> MIME-Version: 1.0 To: dna@merry.microb.uni.wroc.pl Errors-To: fasta-problems@ebi.ac.uk Subject: 116818.1_5 From: EMBL FASTA Server Content-ID: Content-type: TEXT/PLAIN Content-Transfer-Encoding: 7bit Status: RO X-Status: FASTA searches a protein or DNA sequence data bank version 3.3t07 Nov. 2, 2000 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 tmp/0011240958_alpha5_347327.seq: 286 aa >116818.1_5, 286 bases, 251F checksum. vs EBI Borrelia burgdorferi complete proteome library searching /ebi/services/idata/genomes/proteomes/139.FASTAC library 352643 residues in 1251 sequences Expectation_n fit: rho(ln(x))= 6.3358+/-0.00642; mu= 4.2851+/- 0.348 mean_var=147.2160+/-31.644, 0's: 0 Z-trim: 2 B-trim: 57 in 1/39 Lambda= 0.1057 FASTA (3.36 June 2000) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, gap-pen: -12/ -2, width: 16 Scan time: 0.030 The best scores are: opt bits E(1249) SWALL:O51663 (O51663) CDP-DIACYLGLYCEROL--GLYCERO ( 209) 131 31 0.038 SWALL:O51186 (O51186) NA+/CA+ EXCHANGE PROTEIN, P ( 337) 131 31 0.053 SWALL:O51373 (O51373) HYPOTHETICAL 30.8 kDa PROTE ( 259) 103 27 0.85 SWALL:FLIR_BORBU (Q44907) Flagellar biosynthetic ( 269) 102 27 0.97 SWALL:O51476 (O51476) HYPOTHETICAL 71.0 kDa PROTE ( 607) 107 28 0.99 SWALL:O51661 (O51661) ROD SHAPE-DETERMINING PROTE ( 459) 104 27 1.1 SWALL:O51000 (O51000) PTS SYSTEM, MALTOSE AND GLU ( 542) 104 27 1.3 SWALL:O51170 (O51170) GLYCINE BETAINE, L-PROLINE ( 299) 99 26 1.4 SWALL:O51429 (O51429) CONSERVED HYPOTHETICAL INTE ( 454) 100 27 1.7 SWALL:O51694 (O51694) MEMBRANE SPANNING PROTEIN, ( 244) 94 25 2.1 SWALL:FTSW_BORBU (Q44775) Cell division protein F ( 364) 95 26 2.5 SWALL:O51187 (O51187) HYPOTHETICAL 71.4 kDa PROTE ( 614) 97 26 2.9 SWALL:O51747 (O51747) CONSERVED HYPOTHETICAL INTE ( 429) 94 26 3.1 SWALL:O51753 (O51753) HYPOTHETICAL 7.1 kDa PROTEI ( 58) 77 22 4.9 SWALL:O51080 (O51080) CONSERVED HYPOTHETICAL INTE ( 233) 85 24 5.3 SWALL:O51505 (O51505) HYPOTHETICAL 18.1 kDa PROTE ( 155) 81 23 6.2 SWALL:O51296 (O51296) CONSERVED HYPOTHETICAL INTE ( 268) 84 24 6.5 SWALL:O51754 (O51754) PANTOTHENATE PERMEASE (PANF ( 444) 87 25 6.6 SWALL:Y095_BORBU (O51122) Hypothetical protein BB ( 181) 81 23 6.9 SWALL:O51272 (O51272) CELL DIVISION PROTEIN, PUTA ( 787) 90 25 7.1 SWALL:O50880 (O50880) MULTIDRUG-EFFLUX TRANSPORTE ( 392) 85 24 7.5 SWALL:O51521 (O51521) CONSERVED HYPOTHETICAL INTE ( 283) 82 24 8.3 SWALL:O51651 (O51651) HYPOTHETICAL 12.7 kDa PROTE ( 107) 75 22 9.1 SWALL:O51274 (O51274) HYPOTHETICAL 86.3 kDa PROTE ( 737) 87 25 9.3 SWALL:O51707 (O51707) COLICIN V PRODUCTION PROTEI ( 162) 77 23 9.7 >>SWALL:O51663 (O51663) CDP-DIACYLGLYCEROL--GLYCEROL-3-P (209 aa) initn: 67 init1: 67 opt: 131 Z-score: 126.5 bits: 30.9 E(): 0.038 Smith-Waterman score: 131; 26.277% identity (28.800% ungapped) in 137 aa overlap (18-146:20-152) 10 20 30 40 50 116818 LLSKVKRFAFFARLGTFLFFVPLILFLIFLDFKNYLFLNILIF--IFSGFAAKEVNDL : :. ::::.. : .:::..:. : ::. :. .. SWALL: MNNLIKVITPNKITLVRIALSFIILILFFLENVFFSYLFFGIIWFLIIFNEFT-DFIDGY 10 20 30 40 50 60 70 80 90 100 110 116818 LKLKFKFSGLSSILSFFLGFAPPILTYIHFNVFYLGMNVI-YYLFIALVFSNWIVDLV-- : :. :: : .. .: .: .. . ::.. .. ::.:. ... . . .: SWALL: LARKY---GLVSNVGKILDPYADVLQHLTYFVFFFYKGITPYYFFVIFIYREISIGFVRN 60 70 80 90 100 110 120 130 140 150 160 170 116818 FIKEHEI---GNFLSQATSILFILIYPGVLMSFTVSITTLPKAPFLMLMLFAMVSGNDTF .: . .. .:::.. :.:. . . :. .:.. SWALL: LIIQFNVVQQANFLGKLKSLLYAVCTFASLLFYTLNQLNFTESVQNFISYILTFKFKFLF 120 130 140 150 160 170 180 190 200 210 220 230 116818 AYLFGYFLGKNSYRPTIISPNKTLMGFFGGILFSVLTAIFAVVFRLINLSYGESIILGIL SWALL: IVQMTYVCAAFFAILSFLEYVLIFLNVKKYENK 180 190 200 From mailadmn@ebi.ac.uk Fri Nov 24 13:29:47 2000 Received: from alpha1.ebi.ac.uk [193.62.196.122] (root) by merry.microb.uni.wroc.pl with esmtp (Exim 2.05 #1 (Debian)) id 13zHz7-00003l-00; Fri, 24 Nov 2000 13:29:45 +0100 Received: from cardhu.ebi.ac.uk (cardhu.ebi.ac.uk [193.62.196.188]) by alpha1.ebi.ac.uk (8.8.8/8.8.7) with ESMTP id LAA21327 for ; Fri, 24 Nov 2000 11:29:46 GMT Received: (from mailadmn@localhost) by cardhu.ebi.ac.uk (8.10.1/8.10.1) id eAOBTkZ5666249; Fri, 24 Nov 2000 11:29:46 GMT Date: Fri, 24 Nov 2000 11:29:46 GMT Message-Id: <200011241129.eAOBTkZ5666249@cardhu.ebi.ac.uk> MIME-Version: 1.0 To: dna@merry.microb.uni.wroc.pl Errors-To: fasta-problems@ebi.ac.uk Subject: 169399.1_6 From: EMBL FASTA Server Content-ID: Content-type: TEXT/PLAIN Content-Transfer-Encoding: 7bit Status: RO X-Status: FASTA searches a protein or DNA sequence data bank version 3.3t07 Sept. 19, 2000 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 tmp/0011241129_alpha5_311866.seq: 46 aa >169399.1_6, 46 bases, FCF checksum. vs EBI Borrelia burgdorferi complete proteome library searching /ebi/services/idata/genomes/proteomes/139.FASTAC library 352643 residues in 1251 sequences Expectation_n fit: rho(ln(x))= 4.3267+/-0.00397; mu= 2.6162+/- 0.216 mean_var=63.2384+/-12.963, 0's: 1 Z-trim: 2 B-trim: 0 in 0/40 Lambda= 0.1613 FASTA (3.36 June 2000) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, gap-pen: -12/ -2, width: 16 Scan time: 0.130 The best scores are: opt bits E(1248) SWALL:Y310_BORBU (O51290) Hypothetical protein BB ( 45) 80 26 0.038 SWALL:Y410_BORBU (O51371) Hypothetical protein BB ( 39) 66 23 0.33 SWALL:O51217 (O51217) HYPOTHETICAL 88.0 kDa PROTE ( 754) 69 25 1.6 SWALL:O51281 (O51281) FLAGELLAR PROTEIN (FLBD) ( 74) 56 21 2.6 SWALL:O51628 (O51628) 3-HYDROXY-3-METHYLGLUTARYL- ( 431) 61 23 3.9 SWALL:O51582 (O51582) NA+/H+ ANTIPORTER (NHAC-1) ( 449) 61 23 4 SWALL:O51163 (O51163) HYPOTHETICAL 8.4 kDa PROTEI ( 69) 52 20 4.7 SWALL:O50802 (O50802) HYPOTHETICAL 26.9 kDa PROTE ( 228) 56 21 5.6 SWALL:O51041 (O51041) CONSERVED HYPOTHETICAL PROT ( 261) 56 21 6.2 SWALL:O50927 (O50927) OLIGOPEPTIDE ABC TRANSPORTE ( 529) 58 22 7.4 SWALL:DP3B_BORBU (P33761) DNA polymerase III, bet ( 385) 56 21 8.1 >>SWALL:Y310_BORBU (O51290) Hypothetical protein BB0310 (45 aa) initn: 26 init1: 26 opt: 80 Z-score: 126.6 bits: 26.0 E(): 0.038 Smith-Waterman score: 80; 40.000% identity (41.379% ungapped) in 30 aa overlap (13-41:3-32) 10 20 30 40 169399 YYILSYKYLISNFFYNCYLICCSDLIFIIIYNTV-GIYLNYYLIEVL :. . .: :: .. : .:.: : :.:. :: SWALL: MFFLIKIFLGCCLNVWFNLIFNKVRGFYMFYYKDFNVLFIRRVNV 10 20 30 40 >>SWALL:Y410_BORBU (O51371) Hypothetical protein BB0410 (39 aa) initn: 34 init1: 34 opt: 66 Z-score: 109.8 bits: 22.7 E(): 0.33 Smith-Waterman score: 66; 41.176% identity (41.176% ungapped) in 17 aa overlap (11-27:5-21) 10 20 30 40 169399 YYILSYKYLISNFFYNCYLICCSDLIFIIIYNTVGIYLNYYLIEVL :.:: ::..: . ..:. SWALL: MKKRSKFFLYCYILCLTGFLFFSLNPKALKQIKHKFMTI 10 20 30 From mailadmn@ebi.ac.uk Fri Nov 24 14:32:20 2000 Received: from alpha1.ebi.ac.uk [193.62.196.122] (root) by merry.microb.uni.wroc.pl with esmtp (Exim 2.05 #1 (Debian)) id 13zIxg-0000FB-00; Fri, 24 Nov 2000 14:32:20 +0100 Received: from oban.ebi.ac.uk (oban.ebi.ac.uk [193.62.196.251]) by alpha1.ebi.ac.uk (8.8.8/8.8.7) with ESMTP id MAA32456 for ; Fri, 24 Nov 2000 12:32:28 GMT Received: (from mailadmn@localhost) by oban.ebi.ac.uk (8.10.1/8.10.1) id eAOCWSg1839835; Fri, 24 Nov 2000 12:32:28 GMT Date: Fri, 24 Nov 2000 12:32:28 GMT Message-Id: <200011241232.eAOCWSg1839835@oban.ebi.ac.uk> MIME-Version: 1.0 To: dna@merry.microb.uni.wroc.pl Errors-To: fasta-problems@ebi.ac.uk Subject: 206044.1_4 From: EMBL FASTA Server Content-ID: Content-type: TEXT/PLAIN Content-Transfer-Encoding: 7bit Status: RO X-Status: FASTA searches a protein or DNA sequence data bank version 3.3t07 Sept. 19, 2000 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 tmp/0011241232_alpha5_403936.seq: 162 aa >206044.1_4, 162 bases, 1BFD checksum. vs EBI Borrelia burgdorferi complete proteome library searching /ebi/services/idata/genomes/proteomes/139.FASTAC library 352643 residues in 1251 sequences Expectation_n fit: rho(ln(x))= 4.5790+/-0.00592; mu= 9.6363+/- 0.328 mean_var=110.8182+/-22.312, 0's: 0 Z-trim: 3 B-trim: 0 in 0/40 Lambda= 0.1218 FASTA (3.36 June 2000) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, gap-pen: -12/ -2, width: 16 Scan time: 0.230 The best scores are: opt bits E(1248) SWALL:O51651 (O51651) HYPOTHETICAL 12.7 kDa PROTE ( 107) 111 29 0.041 SWALL:FLHB_BORBU (Q44760) Flagellar biosynthetic ( 372) 110 30 0.093 SWALL:O51329 (O51329) HYPOTHETICAL 41.1 kDa PROTE ( 346) 102 28 0.24 SWALL:O51374 (O51374) CONSERVED HYPOTHETICAL INTE ( 207) 89 26 0.87 SWALL:O51531 (O51531) CONSERVED HYPOTHETICAL INTE ( 448) 91 26 1 SWALL:O51328 (O51328) HYPOTHETICAL 71.3 kDa PROTE ( 599) 90 26 1.4 SWALL:O51272 (O51272) CELL DIVISION PROTEIN, PUTA ( 787) 87 26 2.3 SWALL:O51265 (O51265) PHOSPHATIDYLTRANSFERASE ( 234) 81 24 2.5 SWALL:O51426 (O51426) HYPOTHETICAL 8.9 kDa PROTEI ( 75) 70 22 5 SWALL:O51193 (O51193) HYPOTHETICAL 22.9 kDa PROTE ( 195) 73 23 5.9 SWALL:O51519 (O51519) GLYCOSYL TRANSFERASE (LGTD) ( 358) 72 23 9.3 >>SWALL:O51651 (O51651) HYPOTHETICAL 12.7 kDa PROTEIN (107 aa) initn: 81 init1: 54 opt: 111 Z-score: 126.0 bits: 29.0 E(): 0.041 Smith-Waterman score: 111; 32.222% identity (34.524% ungapped) in 90 aa overlap (75-159:15-103) 50 60 70 80 90 100 206044 RRIELLTQGFSVLCSTNWATAAFLFYYLNFFISIYVCQLFLNFSFQMYCYKNDSYLVFRL :: : : :::: .... . :: .: . SWALL: MKAFKVKNLRRFSNFIRILVIVLFLNSLLSLFVFLAGSYNIF-V 10 20 30 40 110 120 130 140 150 206044 YNGQ--FLKIYLIFQGVYYNIF---WVFFYIYIKICIIIYSIIIDYYYIFYRYLKKKFIL :: : .: . .:...: ... .:::. . : : :.: . ::. : :..: SWALL: YNFQKFYLDLAIILSSVSFGLESTRLIFFYFLKNKKIKYYLILIFSFIIFFIALVFKIFL 50 60 70 80 90 100 160 206044 INF SWALL: SGNK From mailadmn@ebi.ac.uk Fri Nov 24 15:35:45 2000 Received: from alpha1.ebi.ac.uk [193.62.196.122] (root) by merry.microb.uni.wroc.pl with esmtp (Exim 2.05 #1 (Debian)) id 13zJx3-0000Nf-00; Fri, 24 Nov 2000 15:35:45 +0100 Received: from cardhu.ebi.ac.uk (cardhu.ebi.ac.uk [193.62.196.188]) by alpha1.ebi.ac.uk (8.8.8/8.8.7) with ESMTP id NAA22740 for ; Fri, 24 Nov 2000 13:35:47 GMT Received: (from mailadmn@localhost) by cardhu.ebi.ac.uk (8.10.1/8.10.1) id eAODZk65655378; Fri, 24 Nov 2000 13:35:46 GMT Date: Fri, 24 Nov 2000 13:35:46 GMT Message-Id: <200011241335.eAODZk65655378@cardhu.ebi.ac.uk> MIME-Version: 1.0 To: dna@merry.microb.uni.wroc.pl Errors-To: fasta-problems@ebi.ac.uk Subject: 245394.3_4 From: EMBL FASTA Server Content-ID: Content-type: TEXT/PLAIN Content-Transfer-Encoding: 7bit Status: RO X-Status: FASTA searches a protein or DNA sequence data bank version 3.3t07 Sept. 19, 2000 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 tmp/0011241335_alpha5_413080.seq: 132 aa >245394.3_4, 132 bases, 1252 checksum. vs EBI Borrelia burgdorferi complete proteome library searching /ebi/services/idata/genomes/proteomes/139.FASTAC library 352643 residues in 1251 sequences Expectation_n fit: rho(ln(x))= 5.3650+/-0.00735; mu= 8.3061+/- 0.400 mean_var=187.8695+/-38.034, 0's: 0 Z-trim: 2 B-trim: 54 in 1/39 Lambda= 0.0936 FASTA (3.36 June 2000) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, gap-pen: -12/ -2, width: 16 Scan time: 0.240 The best scores are: opt bits E(1249) SWALL:O51747 (O51747) CONSERVED HYPOTHETICAL INTE ( 429) 150 31 0.026 SWALL:O51521 (O51521) CONSERVED HYPOTHETICAL INTE ( 283) 132 29 0.11 SWALL:O51277 (O51277) CONSERVED HYPOTHETICAL PROT ( 417) 112 26 0.89 SWALL:O51061 (O51061) SIGNAL PEPTIDASE I (LEPB-1) ( 211) 102 24 1.6 SWALL:O51297 (O51297) CONSERVED HYPOTHETICAL INTE ( 310) 102 25 1.9 SWALL:O51329 (O51329) HYPOTHETICAL 41.1 kDa PROTE ( 346) 100 24 2.5 SWALL:O51250 (O51250) CONSERVED HYPOTHETICAL INTE ( 275) 98 24 2.7 SWALL:O51098 (O51098) HYPOTHETICAL 58.2 kDa PROTE ( 490) 101 25 2.7 SWALL:O51651 (O51651) HYPOTHETICAL 12.7 kDa PROTE ( 107) 92 23 2.9 SWALL:O51359 (O51359) HYPOTHETICAL 40.6 kDa PROTE ( 343) 98 24 3 SWALL:O50999 (O50999) HYPOTHETICAL 49.4 kDa PROTE ( 414) 99 24 3 SWALL:O51266 (O51266) DEDA PROTEIN (DEDA) ( 204) 95 23 3 SWALL:O51335 (O51335) HYPOTHETICAL 16.5 kDa PROTE ( 141) 92 23 3.3 SWALL:O51272 (O51272) CELL DIVISION PROTEIN, PUTA ( 787) 100 25 3.7 SWALL:O50880 (O50880) MULTIDRUG-EFFLUX TRANSPORTE ( 392) 96 24 3.8 SWALL:O51753 (O51753) HYPOTHETICAL 7.1 kDa PROTEI ( 58) 81 21 6 SWALL:O51659 (O51659) CONSERVED HYPOTHETICAL INTE ( 160) 86 22 6.2 SWALL:LSPA_BORBU (O51425) Lipoprotein signal pept ( 170) 85 22 7.1 SWALL:O51538 (O51538) HYPOTHETICAL 28.4 kDa PROTE ( 237) 86 22 7.6 SWALL:FLHB_BORBU (Q44760) Flagellar biosynthetic ( 372) 86 23 9.5 >>SWALL:O51747 (O51747) CONSERVED HYPOTHETICAL INTEGRAL (429 aa) initn: 81 init1: 81 opt: 150 Z-score: 129.7 bits: 31.4 E(): 0.026 Smith-Waterman score: 150; 29.915% identity (32.110% ungapped) in 117 aa overlap (12-122:11-125) 10 20 30 40 50 245394 YLYLLIFITILFFIKKF--YFLIFYSEYYYIFYLNFILFFARWLEFFYFSLFYYKKLIFL ..: .: :::: . ....:..: :::: : : : .: : .::. SWALL: MKILKNSYESYIIAEFFKYFLITFLFFFFVFFINQILFFMRILLQNYVPFF--KAFIFI 10 20 30 40 50 60 70 80 90 100 110 245394 IKALLKLYLISYFYLRLMSII---YEFYFLNYEFLFYNL-FKDFLFYIKIFTLDFFINEV : .: . .: . :.:.: ..: . : . : .. .. : . . .: : . : : SWALL: IYSLPMVIALSPPFASLISVILTIHKFKLHNEILAFRSIGISIFDLLVPFFKLGIVIAFV 60 70 80 90 100 110 120 130 245394 LFVSSSFFYLIFEFIYVI :.:.... SWALL: SFISNDILLPLGSIGRLKIFNEIKEEVPHLVLKPYSSKQYGDLIFVSGEKSENGYKNVTF 120 130 140 150 160 170 From mailadmn@ebi.ac.uk Tue Nov 28 16:59:43 2000 Received: from alpha1.ebi.ac.uk [193.62.196.122] (root) by merry.microb.uni.wroc.pl with esmtp (Exim 2.05 #1 (Debian)) id 140nAU-00055w-00; Tue, 28 Nov 2000 16:59:43 +0100 Received: from balvenie.ebi.ac.uk (balvenie.ebi.ac.uk [193.62.196.242]) by alpha1.ebi.ac.uk (8.8.8/8.8.7) with ESMTP id OAA14290 for ; Tue, 28 Nov 2000 14:59:59 GMT Received: (from mailadmn@localhost) by balvenie.ebi.ac.uk (8.10.1/8.10.1) id eASExxN04671; Tue, 28 Nov 2000 14:59:59 GMT Date: Tue, 28 Nov 2000 14:59:59 GMT Message-Id: <200011281459.eASExxN04671@balvenie.ebi.ac.uk> MIME-Version: 1.0 To: dna@merry.microb.uni.wroc.pl Errors-To: fasta-problems@ebi.ac.uk Subject: 410447.2_6 From: EMBL FASTA Server Content-ID: Content-type: TEXT/PLAIN Content-Transfer-Encoding: 7bit Status: RO X-Status: FASTA searches a protein or DNA sequence data bank version 3.3t07 Sept. 19, 2000 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 tmp/0011281116_alpha5_337794.seq: 113 aa >410447.2_6, 113 bases, 1694 checksum. vs EBI Borrelia burgdorferi complete proteome library searching /ebi/services/idata/genomes/proteomes/139.FASTAC library 352641 residues in 1251 sequences Expectation_n fit: rho(ln(x))= 4.6815+/-0.00647; mu= 6.2367+/- 0.354 mean_var=142.5316+/-29.966, 0's: 0 Z-trim: 1 B-trim: 2 in 1/39 Lambda= 0.1074 FASTA (3.36 June 2000) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, gap-pen: -12/ -2, width: 16 Scan time: 0.200 The best scores are: opt bits E(1250) SWALL:O51329 (O51329) HYPOTHETICAL 41.1 kDa PROTE ( 346) 124 30 0.043 SWALL:Y006_BORBU (O51039) Hypothetical protein BB ( 374) 95 26 1 SWALL:O51617 (O51617) HYPOTHETICAL 41.7 kDa PROTE ( 348) 94 25 1.1 SWALL:MVIN_BORBU (O51750) Virulence factor mviN h ( 512) 92 25 1.6 SWALL:O51335 (O51335) HYPOTHETICAL 16.5 kDa PROTE ( 141) 85 23 1.8 SWALL:PLSC_BORBU (Q59188) 1-acyl-SN-glycerol-3-ph ( 250) 86 24 2.1 SWALL:O51521 (O51521) CONSERVED HYPOTHETICAL INTE ( 283) 85 24 2.5 SWALL:O51543 (O51543) METHYL-ACCEPTING CHEMOTAXIS ( 735) 87 25 3.3 SWALL:LSPA_BORBU (O51425) Lipoprotein signal pept ( 170) 78 22 4.2 SWALL:O51663 (O51663) CDP-DIACYLGLYCEROL--GLYCERO ( 209) 78 23 4.6 SWALL:O51217 (O51217) HYPOTHETICAL 88.0 kDa PROTE ( 754) 84 24 4.6 SWALL:O51678 (O51678) HYPOTHETICAL 5.6 kDa PROTEI ( 47) 68 20 6.4 SWALL:O51277 (O51277) CONSERVED HYPOTHETICAL PROT ( 417) 77 23 7.3 SWALL:O51194 (O51194) HYPOTHETICAL 40.3 kDa PROTE ( 346) 76 23 7.4 SWALL:O51273 (O51273) BACITRACIN RESISTANCE PROTE ( 266) 74 22 8 SWALL:O51734 (O51734) HYPOTHETICAL 168.8 kDa PROT (1465) 80 24 9.9 >>SWALL:O51329 (O51329) HYPOTHETICAL 41.1 kDa PROTEIN (346 aa) initn: 66 init1: 66 opt: 124 Z-score: 125.7 bits: 30.1 E(): 0.043 Smith-Waterman score: 134; 28.302% identity (31.579% ungapped) in 106 aa overlap (1-105:10-105) 10 20 30 40 50 410447 VLWVLYSFNKVFN-FRRLLFKYVLNYHLLYVIIRLFFNVFVVIIVSYEMIF ..: . . :::. :. : . .:...:: :. .: .: SWALL: MKRLKRRLKIFWSITKRLKVFSIFEGGGFAVFDSLRLIFLIICRFILIFC--------LF 10 20 30 40 50 60 70 80 90 100 110 410447 TIFLYWSFMLNFKFRLYFCCFNIFTLVLMYNVVMLSYYLYKFWQLFKLRCAFYYVYFILY .... .:.:..:: ::: :.::. :.::. . :. : .... : :: . SWALL: SLMFICTFYLKYKF-LYFN-FSIFSYSLYYNAYIYSFPLSLVVTFMRISCPFYGIVLNSS 60 70 80 90 100 110 410447 NTL SWALL: RESFYFYCIIFVLILLFSYLGFLVSHSFHSYYINSNRNDNFILKDEIVHFLNDKIIFSSN 120 130 140 150 160 170 From mailadmn@ebi.ac.uk Tue Nov 28 21:26:43 2000 Received: from alpha1.ebi.ac.uk [193.62.196.122] (root) by merry.microb.uni.wroc.pl with esmtp (Exim 2.05 #1 (Debian)) id 140rKt-0005Kf-00; Tue, 28 Nov 2000 21:26:43 +0100 Received: from oban.ebi.ac.uk (oban.ebi.ac.uk [193.62.196.251]) by alpha1.ebi.ac.uk (8.8.8/8.8.7) with ESMTP id TAA29528 for ; Tue, 28 Nov 2000 19:27:00 GMT Received: (from mailadmn@localhost) by oban.ebi.ac.uk (8.10.1/8.10.1) id eASJQxV09303; Tue, 28 Nov 2000 19:26:59 GMT Date: Tue, 28 Nov 2000 19:26:59 GMT Message-Id: <200011281926.eASJQxV09303@oban.ebi.ac.uk> MIME-Version: 1.0 To: dna@merry.microb.uni.wroc.pl Errors-To: fasta-problems@ebi.ac.uk Subject: 444053.2_4 From: EMBL FASTA Server Content-ID: Content-type: TEXT/PLAIN Content-Transfer-Encoding: 7bit Status: RO X-Status: FASTA searches a protein or DNA sequence data bank version 3.3t07 Sept. 19, 2000 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 tmp/0011281300_alpha5_64390.seq: 32 aa >444053.2_4, 32 bases, 58B checksum. vs EBI Borrelia burgdorferi complete proteome library searching /ebi/services/idata/genomes/proteomes/139.FASTAC library 352641 residues in 1251 sequences Expectation_n fit: rho(ln(x))= 3.2292+/-0.00253; mu= 3.2002+/- 0.136 mean_var=29.4858+/- 6.448, 0's: 2 Z-trim: 4 B-trim: 0 in 0/40 Lambda= 0.2362 FASTA (3.36 June 2000) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, gap-pen: -12/ -2, width: 16 Scan time: 0.100 The best scores are: opt bits E(1245) SWALL:Y073_BORBU (O51100) Hypothetical protein BB ( 186) 66 29 0.014 SWALL:O50880 (O50880) MULTIDRUG-EFFLUX TRANSPORTE ( 392) 60 27 0.099 SWALL:MVIN_BORBU (O51750) Virulence factor mviN h ( 512) 57 26 0.25 SWALL:O51174 (O51174) HYPOTHETICAL 3.7 kDa PROTEI ( 30) 45 21 0.48 SWALL:O51296 (O51296) CONSERVED HYPOTHETICAL INTE ( 268) 50 24 0.79 SWALL:O51408 (O51408) HYPOTHETICAL 27.0 kDa PROTE ( 234) 49 23 0.9 SWALL:O51691 (O51691) HYPOTHETICAL 5.7 kDa PROTEI ( 47) 41 20 1.7 SWALL:O51590 (O51590) PTS SYSTEM, GLUCOSE-SPECIFI ( 514) 48 23 2.1 SWALL:O51520 (O51520) ABC TRANSPORTER, ATP-BINDIN ( 270) 43 21 4.1 SWALL:Y845_BORBU (O51785) Hypothetical protein BB ( 35) 36 18 4.5 SWALL:O51328 (O51328) HYPOTHETICAL 71.3 kDa PROTE ( 599) 45 22 4.7 SWALL:O50924 (O50924) CONSERVED HYPOTHETICAL PROT ( 450) 44 22 4.8 SWALL:O50719 (O50719) HYPOTHETICAL 7.4 kDa PROTEI ( 61) 37 19 5.5 SWALL:O51278 (O51278) SIGNAL PEPTIDASE I (LEPB-3) ( 168) 40 20 5.8 SWALL:O51516 (O51516) HYPOTHETICAL 68.1 kDa PROTE ( 590) 44 22 5.9 SWALL:O51236 (O51236) PHOSPHATE ABC TRANSPORTER, ( 260) 41 21 6.4 SWALL:O51241 (O51241) HYPOTHETICAL 13.8 kDa PROTE ( 117) 38 19 7.1 SWALL:O51666 (O51666) K+ TRANSPORT PROTEIN (NTPJ) ( 443) 42 21 7.6 SWALL:O51530 (O51530) CONSERVED HYPOTHETICAL INTE ( 449) 42 21 7.7 SWALL:O51143 (O51143) PTS SYSTEM, MALTOSE AND GLU ( 574) 42 21 9.3 SWALL:RNH2_BORBU (O51075) Ribonuclease HII (EC 3. ( 181) 38 19 9.9 SWALL:O51369 (O51369) PTS SYSTEM, FRUCTOSE-SPECIF ( 625) 42 21 9.9 >>SWALL:Y073_BORBU (O51100) Hypothetical protein BB0073 (186 aa) initn: 40 init1: 40 opt: 66 Z-score: 134.6 bits: 29.0 E(): 0.014 Smith-Waterman score: 66; 30.769% identity (30.769% ungapped) in 26 aa overlap (3-28:60-85) 10 20 30 444053 RIKQDCIFQQPILYTICIYYIVFSYEIMYITK :.. .: ::.:.: :: ... .... SWALL: NIMLIKRYAIITITSLLIYSISFYLYKLFAKNNLLFLVPIFYVILIYVLILLFKVLNDLF 30 40 50 60 70 80 SWALL: IVYNKKSNYSNDFMLSNSSLIAITFFALDKNNGFFEGLEILILSALGILIALMSITSIKK 90 100 110 120 130 140 >>SWALL:O50880 (O50880) MULTIDRUG-EFFLUX TRANSPORTER (392 aa) initn: 51 init1: 51 opt: 60 Z-score: 119.1 bits: 27.2 E(): 0.099 Smith-Waterman score: 60; 36.000% identity (36.000% ungapped) in 25 aa overlap (7-31:66-90) 10 20 30 444053 RIKQDCIFQQPILYTICIYYIVFSYEIMYITK ::.. :.: . :. .. :: :.. :. SWALL: GLSLKDIAMVQICYMVAIIIFEFPSGVISDIFDRKIVYLVSIFLLMTSYFIVFKTSSFVF 40 50 60 70 80 90 SWALL: ICVSWFIYGMSAAISTGTIDISFTKIYQNNSKKLKAFISFVKIILSIGAILGGYIGSVLY 100 110 120 130 140 150 From mailadmn@ebi.ac.uk Wed Nov 29 11:46:11 2000 Received: from alpha1.ebi.ac.uk [193.62.196.122] (root) by merry.microb.uni.wroc.pl with esmtp (Exim 2.05 #1 (Debian)) id 1414kd-0005YQ-00; Wed, 29 Nov 2000 11:46:11 +0100 Received: from cardhu.ebi.ac.uk (cardhu.ebi.ac.uk [193.62.196.188]) by alpha1.ebi.ac.uk (8.8.8/8.8.7) with ESMTP id JAA28366 for ; Wed, 29 Nov 2000 09:46:29 GMT Received: (from mailadmn@localhost) by cardhu.ebi.ac.uk (8.10.1/8.10.1) id eAT9kSt27700; Wed, 29 Nov 2000 09:46:28 GMT Date: Wed, 29 Nov 2000 09:46:28 GMT Message-Id: <200011290946.eAT9kSt27700@cardhu.ebi.ac.uk> MIME-Version: 1.0 To: dna@merry.microb.uni.wroc.pl Errors-To: fasta-problems@ebi.ac.uk Subject: 491927.2_2 From: EMBL FASTA Server Content-ID: Content-type: TEXT/PLAIN Content-Transfer-Encoding: 7bit Status: RO X-Status: FASTA searches a protein or DNA sequence data bank version 3.3t07 Sept. 19, 2000 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 tmp/0011281720_alpha5_393588.seq: 220 aa >491927.2_2, 220 bases, 1E5B checksum. vs EBI Borrelia burgdorferi complete proteome library searching /ebi/services/idata/genomes/proteomes/139.FASTAC library 352641 residues in 1251 sequences Expectation_n fit: rho(ln(x))= 5.1817+/-0.00627; mu= 9.7703+/- 0.348 mean_var=130.7529+/-27.194, 0's: 0 Z-trim: 1 B-trim: 57 in 1/39 Lambda= 0.1122 FASTA (3.36 June 2000) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, gap-pen: -12/ -2, width: 16 Scan time: 0.310 The best scores are: opt bits E(1250) SWALL:O51272 (O51272) CELL DIVISION PROTEIN, PUTA ( 787) 135 33 0.027 SWALL:O51521 (O51521) CONSERVED HYPOTHETICAL INTE ( 283) 100 27 0.75 SWALL:O51661 (O51661) ROD SHAPE-DETERMINING PROTE ( 459) 102 27 0.8 SWALL:O51754 (O51754) PANTOTHENATE PERMEASE (PANF ( 444) 95 26 1.7 SWALL:O51329 (O51329) HYPOTHETICAL 41.1 kDa PROTE ( 346) 91 25 2.3 SWALL:O51335 (O51335) HYPOTHETICAL 16.5 kDa PROTE ( 141) 83 23 3.4 SWALL:O51186 (O51186) NA+/CA+ EXCHANGE PROTEIN, P ( 337) 85 24 4.5 SWALL:O51373 (O51373) HYPOTHETICAL 30.8 kDa PROTE ( 259) 83 24 4.8 SWALL:O51277 (O51277) CONSERVED HYPOTHETICAL PROT ( 417) 84 24 5.7 SWALL:O51538 (O51538) HYPOTHETICAL 28.4 kDa PROTE ( 237) 80 23 6.4 SWALL:O51659 (O51659) CONSERVED HYPOTHETICAL INTE ( 160) 74 22 10 >>SWALL:O51272 (O51272) CELL DIVISION PROTEIN, PUTATIVE (787 aa) initn: 50 init1: 50 opt: 135 Z-score: 129.3 bits: 32.9 E(): 0.027 Smith-Waterman score: 135; 26.471% identity (32.143% ungapped) in 170 aa overlap (43-198:10-163) 20 30 40 50 60 70 491927 KMFLIEGKDKVLRGWIVNENILHYFLLLGLFVILFSYSVI-WSIIRVLVFLSNIINFFIL :...: ::: .:.. .:. :::.. ::.. SWALL: MKDFYQYFQFLFFFMLSVISFSLFVALTPLSNVFVFFVF 10 20 30 80 90 100 110 120 491927 YFKDLSVKNNIERLIKICREVFLILNFY---FYNIN-YSFKYSIYFSALIR--RMVLILL . : .: .:: .:.:: . .: :..: :. . .: ...::. SWALL: NL-----------LGQILLNVFSFLSFYLIVYPIVNWYAYKKHIFTKRFIFNWNYTVILF 40 50 60 70 80 130 140 150 160 170 180 491927 ITVYLWLVLELYIFIIYYIWFYFFCFWLWFFCCGRLIDFIYILVIYCITCWLLIVLILY- .:. . . .. . :.: .... : . . : . ::. :.: .. :. . ... SWALL: FTLIFLIKINSNVEKSYFIKIFLINFGIVL---GNF--FIFTLLILEFVVWIYLNYVFFK 90 100 110 120 130 140 190 200 210 220 491927 -INFY-----FLKLKIKYLFITDEYIWFYIFYKKCFRWGYNF .:: ::..::: :: SWALL: DVNFILDTFKFLEFKIKILFENILSYFPFSNSLDVKKDIKVYGDSVDDLKDSQVFDEKKN 150 160 170 180 190 200 From mailadmn@ebi.ac.uk Wed Nov 29 11:47:33 2000 Received: from alpha1.ebi.ac.uk [193.62.196.122] (root) by merry.microb.uni.wroc.pl with esmtp (Exim 2.05 #1 (Debian)) id 1414lx-0005Yg-00; Wed, 29 Nov 2000 11:47:33 +0100 Received: from cardhu.ebi.ac.uk (cardhu.ebi.ac.uk [193.62.196.188]) by alpha1.ebi.ac.uk (8.8.8/8.8.7) with ESMTP id JAA28447 for ; Wed, 29 Nov 2000 09:47:51 GMT Received: (from mailadmn@localhost) by cardhu.ebi.ac.uk (8.10.1/8.10.1) id eAT9lor27985; Wed, 29 Nov 2000 09:47:50 GMT Date: Wed, 29 Nov 2000 09:47:50 GMT Message-Id: <200011290947.eAT9lor27985@cardhu.ebi.ac.uk> MIME-Version: 1.0 To: dna@merry.microb.uni.wroc.pl Errors-To: fasta-problems@ebi.ac.uk Subject: 494542.1_1 From: EMBL FASTA Server Content-ID: Content-type: TEXT/PLAIN Content-Transfer-Encoding: 7bit Status: RO X-Status: FASTA searches a protein or DNA sequence data bank version 3.3t07 Sept. 19, 2000 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 tmp/0011281730_alpha5_394494.seq: 157 aa >494542.1_1, 157 bases, 1A43 checksum. vs EBI Borrelia burgdorferi complete proteome library searching /ebi/services/idata/genomes/proteomes/139.FASTAC library 352641 residues in 1251 sequences Expectation_n fit: rho(ln(x))= 5.1820+/-0.00621; mu= 8.2966+/- 0.341 mean_var=132.3980+/-27.459, 0's: 0 Z-trim: 3 B-trim: 0 in 0/40 Lambda= 0.1115 FASTA (3.36 June 2000) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, gap-pen: -12/ -2, width: 16 Scan time: 0.250 The best scores are: opt bits E(1248) SWALL:O51329 (O51329) HYPOTHETICAL 41.1 kDa PROTE ( 346) 136 32 0.013 SWALL:O51663 (O51663) CDP-DIACYLGLYCEROL--GLYCERO ( 209) 118 29 0.075 SWALL:O51538 (O51538) HYPOTHETICAL 28.4 kDa PROTE ( 237) 101 26 0.54 SWALL:O51537 (O51537) COMPETENCE LOCUS E, PUTATIV ( 416) 98 26 1 SWALL:O51194 (O51194) HYPOTHETICAL 40.3 kDa PROTE ( 346) 94 25 1.5 SWALL:O51150 (O51150) HYPOTHETICAL 10.9 kDa PROTE ( 92) 83 23 2.3 SWALL:O51250 (O51250) CONSERVED HYPOTHETICAL INTE ( 275) 87 24 2.8 SWALL:O51521 (O51521) CONSERVED HYPOTHETICAL INTE ( 283) 85 24 3.5 SWALL:O51426 (O51426) HYPOTHETICAL 8.9 kDa PROTEI ( 75) 77 22 4 SWALL:FLIR_BORBU (Q44907) Flagellar biosynthetic ( 269) 81 23 5.4 SWALL:O51295 (O51295) HYPOTHETICAL 26.9 kDa PROTE ( 228) 80 23 5.5 SWALL:Y089_BORBU (O51116) Hypothetical protein BB ( 324) 78 23 8.3 SWALL:O51298 (O51298) EXPORTED PROTEIN (TPN38B) ( 350) 78 23 8.7 >>SWALL:O51329 (O51329) HYPOTHETICAL 41.1 kDa PROTEIN (346 aa) initn: 61 init1: 61 opt: 136 Z-score: 134.7 bits: 32.2 E(): 0.013 Smith-Waterman score: 136; 30.935% identity (36.752% ungapped) in 139 aa overlap (6-141:24-143) 10 20 30 40 494542 FLLYFFTGESVNLFDKIKVFDQFYIVTRFQKVFTIVLNFMFL : : . .::..... : :. :: .: . ...::. SWALL: MKRLKRRLKIFWSITKRLKVFSIFEGGGFAVFDSLRLI--FLIICRFILIFCL-FSLMFI 10 20 30 40 50 50 60 70 80 90 100 494542 NVLVEHCLFYVVAFKALFFNILCFSLINVKFILIFNAIICV--VNWVYKFKRIDYILYNV : ::. .: :.::. :: . : .:: : .. : : ::. .:.. SWALL: ------CTFYL-KYKFLYFNFSIFS-----YSLYYNAYIYSFPLSLVVTFMRISCPFYGI 60 70 80 90 100 110 120 130 140 150 494542 YNFKAIKIFYKNTYCGSISKKLIFLFFWL-FVAIFLFYYEYICYKFFKEIKCKYLFRI .. . :: :: : ::.:: .: :.. :. :: SWALL: VLNSSRESFY--FYC--IIFVLILLFSYLGFLVSHSFHSYYINSNRNDNFILKDEIVHFL 110 120 130 140 150 160 SWALL: NDKIIFSSNRPRIYGFNGVLIVSENDKGDKGFSYQSNISNSSKIDFVENNFLEQKIYNNF 170 180 190 200 210 220