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["Additional file 1 – Y-chromosome markers examined in this study."]
["Haplogroup","Marker","rs_SNP","Forward_Primer 5'->3'","Reverse_Primer 5'->3'","Primer Reference","Fragment Size (bp)","Mutation","Mutated position (nt)","Type of analysis","Enzyme"," Allele Fragments (bp)"]
["DE","YAP"," -","caggggaagataaagaaata","actgctaaaaggggatggat ","[1]","455(Alu+) 150(Alu-)","Alu-→Alu+",null,"AFLP",null,"(-) 150; (+) 455"]
["E","M2","rs3893","aggcactggtcagaatgaag","aatggaaaatacagctcccc","[2]",209,"A→G",168,"RFLP"," Hsp92II or NlaIII ","(A) 102/65/42; (G) 144/65"]
["E","M123","-","cacagtatctgaactagcatctca","cagcgaattagattttcttgc","[3]",257,"G→A",25,"RFLP","DdeI ","(G)  102/76/58/21; (A) 123/76/58"]
["E","M34","-","cacttcacatttgtttttagg","agtcattatttagtcattccag","[4]",372,"G→T",131,"RFLP","HinfI ","(G) 271/101; (T) 143/128/101"]
["E","M35","-","taagcctaaagagcagtcagag","cagagggagcaatgaggaca","[4]",514,"G→C",168,"DHPLC"]
["E","M78","-","cttcaggcattattttttttggt","atagtgttccttcacctttcctt","[4]",301,"C→T",197,"RFLP","AciI","(C) 196/105; (T) 301 "]
["E","M81","rs2032640","actta tttatagtttcaatccctca","ttcatggagatgtctgtatctgg","[4]",422,"C→T",147,"RFLP ","HpyCH4IV ","(C) 276/146; (T) 422"]
["E","M293","rs9341316","gatattagtattgaagaaacc"," gctggctaatacttccacagag","[5]",338,"T→G",130,"DHPLC"]
["E","PN2","rs9785756","ggtaacacccataaaggttg","ttcactaccagcctaagtac","[4]",247,"C→T",94,"DHPLC"]
["E","SRY4064","rs9786608"," gtataataggctgggtgctg  ","catgagttcaaatgattctt","[4]",218,"G→A",62,"DHPLC"]
["E","V13","-","ggatgctgctaaacatcctaca","atcccatctcaatcccttaaca","[6]",235,"G→A",192,"RFLP","AciI ","(G) 190/45; (A) 235"]
["F","M89","rs2032652","agaagcagattgatgtcccact","tccagttaggagatcccctca","[7]",527,"C→T",347,"DHPLC"]
["G","M201","rs2032636","tatgcatttgttgagtatatgtc","gttctgaatgaaagttcaaacg","[4]",326,"G→T",136,"DHPLC"]
["I","M170","rs2032597","tgcttcacacaaatgcgttt","ccaattactttcaacatttaagacc","[4]",405,"A→C",327,"DHPLC"]
["I","M223"," -","ttcagcaagagtaagcaagagg","cctttttggatcatggttctt","[4]",208,"C→T",67,"DHPLC"]
["I","M253","rs9341296","gcaacaatgagggtttttttg","cagctccacctctatgcagttt","[8]",400,"C→T",283,"RFLP","HincII ","(C)280/120; (T)  400"]
["I","M258","rs9341297","tatatagcatatgttaaatgtttaggt","gacttttgaataatttgcatctttc","[9]",475,"T→C",123,"DHPLC"]
["I","P37.2"," -","cgtctatggccttgaaga","tccgaaaatgcagacttt","[10]",447,"T→C",135,"RFLP","TaaI or HpyCH4III ","(T) 447; (C) 311/136"]
["I-J","M429","rs17306671","gatcatcctaaatgtttacg","ttccatatacatgcataacc","[11]",421,"T→A",233,"DHPLC"]
["J","M304","-","caaagtgctgggattacagg","cttctagcttcatctgcattgt","[8]",527,"A→C",421,"DHPLC"]
["J","M267","rs9341313","ttatcctgagccgttgtccctg","tgtagagacacggttgtaccct","[8]",287,"T→G",148,"DHPLC"]
["J","M365"," -","ccttcatttaggctgtagctgc","tgtatctttagttgagatgg","[8]",274,"A→G",246,"DHPLC"]
["J","M367"," -","ccttcatttaggctgtagctgc","tgtatctttagttgagatgg","[8]",274,"A→G",196,"DHPLC"]
["J","M368"," -","ccttcatttaggctgtagctgc","tgtatctttagttgagatgg","[8]",274,"A→C",200,"DHPLC"]
["J","M369"," -","ccttcatttaggctgtagctgc","tgtatctttagttgagatgg","[8]",274,"G→C",45,"DHPLC"]
["J","PAGE8","rs34043621","acgtcacccatctcaacatc","aaagaatgtctccccatgagg","[13]",306,"T→C",189,"RFLP","DdeI ","(T) 158/69/49/30; (C) 207/69/30"]
["J","M172","rs2032604","aaattaggagccagatgacc","aataataattgaagaccttttgagt","[3]",176,"T→G",151,"RFLP","HinfI ","(T) 176; (G) 151/25"]
["J","M410","-","caatcattgaccttaagtctgagtccc","actggatacctttctaggaagaattg","[14]",395,"A→G",115,"DHPLC"]
["J","M47","-","agatcatcccaaaacaatcataa","aaggctgaaatcaatccaatctg","[4]",436,"G→A",395,"DHPLC"]
["J","M67","rs2032628"," ccatattctttatactttctacctgc    "," gtcttttcacttgttcgtggac ","[4]",409,"A→T",377,"DHPLC","SspI  ","(A) 143/22; (T) 165"]
["J","M68","-","ccagtcagcagtacaaaagttg","gcatttctttgattatagaagcaa","[4]",386,"A→G",219,"DHPLC"]
["J","M92","rs2032648","ttgaatttcccagaattttgc","ttcagaaactggttttgtgtcc","[4]",470,"T→C",340,"DHPLC"]
["J","M102","rs2032608","aaactgggacacttgtaatgaat","gttttttgagtccttgtttattctt","[4]",480,"G→C",301,"DHPLC"]
["J","M12","rs3903","actaaaacaccattagaaacaaagg"," ctgagcaacatagtgacccc","[4]",309,"G→T",286,"DHPLC","DpnII ","(G) 82/74/24; (T) 106/74"]
["J","M241","rs8179022","aactcttgataaaccgtgctg","tccaatctcaattcatgcctc","[8]",366,"G→A",57,"DHPLC"]
["L","M20","rs3911","gattgggtgtcttcagtgct","cacacaacaaggcaccatc","[12]",413,"A→G",118,"RFLP","SspI ","(A) 117/296; (G) 413"]
["L","M76","-","tagaagtagcagattgggagagg","cctgataaaatgaaaaaaatggtc","[4]",493,"T→G",339,"DHPLC"]
["L","M357","-","ccccgttttttcctctctgcc","cacgtaacctgggatggtcata","[14]",459,"C→A",234,"DHPLC"]
["L","PK3"," -","tgtgtcctggtgtcttttgg","tgacaacagcatctcttaactgg","[15]",466,"T→C",365,"DHPLC"]
["P","M45","rs2032631","gctggcaagacacttctga","aatatgttcctgacaccttcc","[7]",352,"G→A",109,"DHPLC"]
["P","M74"," rs2032635","atgctataataactaggtgttgaag","aattcagcttttaccacttctgaa","[4]",385,"G→A",195,"RFLP ","HpyCH4V ","(G) 195/190; (A) 385"]
["Q","M242","rs8179021","aactcttgataaaccgtgctg","tccaatctcaattcatgcctc","[8]",366,"C→T",180,"RFLP","AlW21I or BsiHKAI","(C) 187/179 (T) 366"]
["Q","M25","-","aaagcgagagattcaatccag","ttttagcaagttaagtcaccagc","[4]",340,"G→C",121,"DHPLC"]
["Q","M378","-","tatgcatttgttgagtatatgtc","gttctgaatgaaagttcaaacg","[14]",326,"A→G",114,"DHPLC"]
["Q","P36.2","-","tgaaggacagtaagtacaca","taagtccattgatctacaga","[10]",637,"G→T",217,"Sequencing"]
["R","L23","rs9785971","cctgtagtcccagctactcagg","tatccaatctgccatcagtg","[16]",305,"G→A",122,"RFLP","PflFI ","(G) 186/119; (A) 305"]
["R","M124","-","tggtaaactctacttagttgccttt","cagcgaattagattttcttgc","[4]",393,"C→T",246,"DHPLC"]
["R","M17","rs3908","ctggtcataacactggaaatc","tgaacctacaaatgtgaaactc","[12]",333,"4G→3G",68,"DHPLC"]
["R","M173","rs2032624","aagaaatgttgaactgaaagttgat","aggtgtatctggcatccgtta","[4]",417,"A→C",191,"DHPLC"]
["R","M207","rs2032658","aggaaaaatcagaagtatccctg","caaaattcaccaagaatccttg","[4]",423,"A→G",79,"RFLP","DraI ","(A) 77/346; (G) 423"]
["R","M269","rs9786153","ctaaagatcagagtatctccctttg","atttctaggagttcactgtattac","[17]",379,"T→C",358,"RFLP","Tsp509 I ","(T)251/42/61/10/10/5; (C)  251/42/71/10/5"]
["R","M412","rs9786140 ","agacactagcagtcttgtcctcag","caggtatggaagtgctcaaatcg","[16]",386,"G→A",178,"RFLP","NlaIII","(G) 211/100/75; (A) 180/100/75/31"]
["R","SRY10831.2","rs2534636","tccttagcaaccattaatctgg","aaatagcaaaaaatgacacaaggc","[10]",167,"G → A",115,"RFLP","DraIII  ","(G) 112/55;  (A) 167; "]
["T","M70","rs2032672","ggttatcatagcccactatactttg","atctttattccctttgtcttgct","[4]",257,"A→C",45,"RFLP","BsaI ","(A) 257; (C) 222/35"]
[]
["References for Additional file 1"]
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["5. Henn BM, Gignoux C, Lin AA, Oefner PJ, Shen P, Scozzari R, Cruciani F, Tishkoff SA, Mountain JL, Underhill PA: Y-chromosomal evidence of a pastoralist migration through Tanzania to southern Africa. Proc Natl Acad Sci U S A. 2008, 105:10693-10698."]
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["8. Cinnioğlu C, King R, Kivisild T, Kalfoglu E, Atasoy S, Cavalleri GI, Lillie AS, Roseman CC, Lin AA, Prince K, Oefner PJ, Shen P, Semino O, Cavalli-Sforza LL, Underhill PA: Excavating Y-chromosome haplotype strata in Anatolia. Hum Genet 2004, 114:127-148."]
["9. Rootsi S, Magri C, Kivisild T, Benuzzi G, Help H, Bermisheva M, Kutuev I, Barać L, Pericić M, Balanovsky O, Pshenichnov A, Dion D, Grobei M, Zhivotovsky LA, Battaglia V, Achilli A, Al-Zahery N, Parik J, King R, Cinnioğlu C, Khusnutdinova E, Rudan P, Balanovska E, Scheffrahn W, Simonescu M, Brehm A, Goncalves R, Rosa A, Moisan JP, Chaventre A, Ferak V, Füredi S, Oefner PJ, Shen P, Beckman L, Mikerezi I, Terzić R, Primorac D, Cambon-Thomsen A, Krumina A, Torroni A, Underhill PA, Santachiara-Benerecetti AS, Villems R, Semino O: Phylogeography of Y-chromosome haplogroup I reveals distinct domains of prehistoric gene flow in Europe. Am J Hum Genet 2004, 75:128-137"]
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["17. Cruciani F, Santolamazza P, Shen P, Macaulay V., Moral P, Olckers A, Modiano D, Holmes S, Destro-Bisol G, Coia V, Wallace D C, Oefner P J, Torroni A, Cavalli-Sforza L L, Scozarri R, Underhill P A: A back migration from Asia to sub-Saharan Africa is supported by high-resolution analysis of human Y-chromosome haplotypes. Am J Hum Genet 2002, 70:1197-1214"]