>Saccoglossus kowalevskii (see Additional table 1 for accession details)
MACGATLKRTLDFDPLHSSPSPKRRRCVPIIPP---------------TTPQTKAHKLEP
SPFGQVSPKLTPEQLSNSVLLEYKRMQRRKKLATAFTSGTSTYPAPGPSHQQQQSAAPSS
SPSRREQPIFTLRQVIMLCERLLKEREEQIKAEYDKTLTCKLAEQYEAFLKFNHDQLHRR
FGETAASYVS
>Strongylocentrotus purpuratus (XM_001182037) 
MAC-ATLKRTLDFDPLHSGTSPKRRRCSPIITPSTPTS----------------KSSLQQ
SPFSPVTPRISPGQLVAHISHEWRRIRRRKRLEESYSSEAPSSSEMVETSGCFSPPPSLQ
QTQTKDQPMFTLKQVILICEQMLKEQESRIREEYDKALSCKLAEQYDAFVKFNQDQIQRR
FTSTSMSYVS
>Branchiostoma_floridae(FE572464)
MAC-ATLKRTYELDPLHSRPSKRPRRCMPMAGHSPNVSPT--------------PPRQKP
SSFPEVEPKLTSEQISQFIRDEYRRMHRRRRLWGATDSNVQSAASPSHHGSLSPMSPGTQ
SPINKDKPLFSMRQVSMICERMIKERDTQVREEYDKVLSCKLAEQYDAFIRFNQDYLQRR
FAETAASYVS
>Halocynthia_roretzi(DB60812)
MACGATLKRSLDFDPLHAVSPNKRRRLSPRSGSVYTLGEISTL-----------QRSPPS
SPFKNSAQTLSSGQILKNIYDEYKKMKHRKQLMMNPQAPHDLASNVPSASSFLLSQPPQT
EKKITETPLLTMKQVGLICERLLKEQEEKLRSEYEKALVAKLSEQYDAFVKFNYDQVQRR
ISEHPASYVS
>Homo_sapiens_Akirin1(AK022728)
MACGATLKRPMEFEAALLPGSPKRRRCAPLPGPTPGLRPEPPPPFQTQTPPQS---LQQP
APPGSERRLPTPEQIFQNIKQEYSRYQRWRHLEVVLNQ-SEACASESQPHSSALTAPPGS
SWMKKDQPTFTLRQVGIICERLLKDYEDKIREEYEQILNTKLAEQYESFVKFTHDQIMRR
YGTRPTSYVS
>Mus_musculus_Akirin1(BT028639) 
MACGATLKRPMEFEAALLPGSPKRRRCAPLPGPTPGLRPAEPPPLQMQTPPAS---LQQP
APPGSERRLPTPEQIFQNIKQEYNRYQRWRHLEVVLSQ-SEACTSETQPSSSALTAPPGA
FWMKKDQPTFTLRQVGIICERLLKDYEDKVREEYEQILSTKLAEQYESFVKFTHDQIMRR
YGTRPTSYVS
>Xenopus_tropicalis_Akirin1(DC122883)  
MACGATLKRSMEFE-ALMPQSPKRRRCAPLPGSPATPSP-QRCAIRPEMQQGQ---QQPL
SQLGGDRRLT-PEQILQNIKQEYTRYQRRRQLEGAFNQCEAGALNEVQASCSSLTAPSPG
SLVKKDQPTFSLRQVGILCERLLKDHEDKIREEYEQILNIKLAEQYESFVKFTHDQIMRR
YGARPASYVS
>Danio_rerio_Akirin1(BT028639)   
MACGATLKRSMEFE-ALLPQSPKRRRCNPLPGTPTTPSPQRCSLRPSTESPAH---SMSP
QSMGGEHRLT-PEQIFQNIRQEYSRYQRRRQLEGAFNQTEPCSSTDIQSSSPALTSPTGA
SSLKKDQPLFTLRQVGYLCERLIKDHEEKMREEYEQILNTKLAEQYESFVKFTQDQIMRR
YGARPASYVS
>Pimephales_promelas_Akirin1(DT103169) 
MACGATLKRSMEFE-ALLPQSPKRRRCNPLPGTPTTPSPQRCGLRPSTESPAH---SMSP
QSMAGEHRLT-PEQIFQNIRQEYSRYQRRRQLEGAFNQTEPCSSTDIQSSSPALTSPTGA
STLKKDQPLFTLRQVGYLCERLIKDHEEKMREEYEQILNTKLAEQYESFVKFTQDQIMRR
YGARPASYVS
>Takifugu_rubripes_Akirin1(Ensembl_gene_ID:ENSTRUG00000008943)  
MACGATLKRSMEFE-ALLPQSPKRRRCNPLPGTPSTPSPQRCNLRPPVDSPTH---SMSP
SAIGGEHRLT-PEQIFQNLRQEYSRIQRRRQLEGAFNQTEACSSSDAPSPSSSLHAPPPA
GASRKDQPSFTLKQVSYLCERLLKDHEEKIREEYEQILNTKLAEQYESFVKFTQDQIMRR
YGARPASYVS
>Gasterosteus_aculeatus_Akirin1(BT028639)  
MACGATLKRSMEFE-ALLPQSPKRRRCNPLPGTSGTPSPQRCNLRPPVDSPAH---SMSP
PAMGGEHRLT-PEQIFQNLRQEYSRIQRRRQLEGAFNQTEACSSSDSPSPSSSINAPSPP
GASRKDQPSFTLKQVSYLCERLLKDHEEKIREEYEQILNTKLAEQYESFVKFTQDQIMRR
YGARPASYVS
>Oryzias_latipes__Akirin1(Ensembl_gene_ID:ENSORLG00000014770) 
MACGATLKRSMEFE-ALLPQSPKRRRCNPLPGTPSTPSPQRCNLRPPIDSPTH---SMSP
QPIGGEHRLTPVEQIFQNLRQEYSRIQRRRQLEGAFNQAEACSSSDAPSPSSSLTAPSPP
GASRKDQPSFTLRQVSLLCERLLKDHEEKIREEYEQILNTKLAEQYESFVKFTQDQIMRR
YGARPASYVS
>Petromyzon_marinus_Akirin1(EC384219)  
MACGATLKRQLDFEPLLSSQSPKRRRCTPLSPPSPTSSGYLQAEPTASLGESHNYH-LHS
PAAAA---RLTPEQVLANLKQEYSRMQRRLQVDGPPDA--------HAGDSASMQSGVSG
DGGRREQPLFTLRQVGLICDGLLREREVHIRQEYDQILSTKLAEQYEGFVKFTQDQIMRK
YGERPASYVS
>Homo_sapiens_Akirin2(NM_018064)      
MACGATLKRTLDFDPLLSPASPKRRRCAPLSAPTSAAASATAASFSAAAASPQKYLRMEP
SPFGDVSSRLTTEQILYNIKQEYKRMQKRRHLETSFQQTDPCCTSDAQPHAFLLSGPAAS
SPLKKEQPLFTLRQVGMICERLLKEREEKVREEYEEILNTKLAEQYDAFVKFTHDQIMRR
YGEQPASYVS
>Mus_musculus_Akirin2(NM_001007589)  
MACGATLKRTLDFDPLLSPASPKRRRCAPLSAPASAAASAAAA--SAAAASPQKYLRMEP
SPFGDVSSRLTTEQILYNIKQEYKRMQKRRHLEASFQQADPGCTSDSQPHAFLISGPATS
SPLKKEQPLFTLRQVGMICERLLKEREEKVREEYEEILNTKLAEQYDAFVKFTHDQIMRR
YGEQPASYVS
>Gallus_gallus_Akirin2(BX934526)   
MACGATLKRTLDFDPLLSPASPKRRRCAPLSAPASAAAS------SSSFASPQKYLRMEP
SPFGEVSSRLTTEQILYNIKQEYKRMQKRRHLENSFQQTDPCCSTDAQPHAFLLTGPAAS
SPLKKEQPLFTLRQVGMICERLLKEREEKIREEYEEILTTKLAEQYDAFVKFTHDQIMRR
YGEQPASYVS
>Xenopus_tropicalis_Akirin2(CR760919)   
MACGATLKRTIEFDPLLSAASPKRRRCAPLSPP---------------GPSPQKYLRMEP
SPFGEVSPRLTAEQILYNIKQEYKRMQKRRHLEASFQPTDPCCSSEGQPQTFIPSGPSAT
SPLRKEQPLFSLRQVGMICERLLKEREDKVREEYEEILTTKLAEQYDAFVKFTHDQIMRR
FGEQPASYVS
>Danio_rerio_Akirin2(1)(BC065319)  
MACGATLKRTMDFDPLMNPASPKRRRCAPMSPSSS-------------SASPQKYLRMEP
SPFGRVSSALTTEQILSNIKQEYKRMQKRRHLESSFQQ----TDSCCPLDSQPHASSGSA
SPSRREQPLFTLRQVGMICERLLKEREEKVREEYDEILTTKLAEQYDAFVKFTHDQLMRR
FGEQPASYVS
>Pimephales_promelas_Akirin2(1)(DT344888) 
MACGATLKRTMDFDPLLNQASPKRRRCAPLSPSSS-------------SASPQKYLRMEP
SPFGRVSSALTTEQILSNIKQEYKRMQKRRHLESSFQQ-----SESVCGLESGPHASGAV
SPSRREQPLFTLKQVGMICERLLKEREEKVREEYDEILTTKLAEQYDAFVKFTHDQLMRR
FGEQPASYVS
>Sparus_aurata_Akirin2(1)(AM975928)
MACGATLKRTMDFDPLMSPTSPKRRRCIPVSPSSS-------------SSSPRKYLSMEP
SPFGESSSTLSAEQILNSIKQEYKRIQKRKHLDGGYQQSECCYSPESPSQSSTMNVSGGV
SPTRKEQPLFTLRQVGMICERLLKEREEKVREEYEETMTSKLAEQYDTFVEFTHDQLMRR
FGEQPASYVS
>Oryzias_latipes_Akirin2(1)chr22(Ensembl_gene_ID:ENSORLG00000014760) 
MACGATLKRSMDFDPLTNPTSPKRRRCIPVTPS---------------SAAPRKYLRMEP
SPFGESSSKLSLEQILHSIKQEYKRIHKRKHLDGSYHQ-----SECCYSPESPSQSSGGV
SPTRKEQPLFTLRQVSMICERLLKEREEKVREEYEETMTSKLAEQYDTFVKFTHDQLMRR
FGEQPASYVS
>Takifugu_rubripes_Akirin2(1)scaf10(Ensembl_gene_ID:ENSTRUG00000006920) 
MACGATLKRTMDFDPLMSPTSPKRRRCVPVSTS---------------SPSPRKYLRMEP
SPFGEFSSTISAEQILQSIKQEYKRIQKRKHLDGGYLQSDCSYSPESPSQSSSMHISGGV
SPTRKEQPLFTLRQVGIICERLLKEREEKVREEYEETMTSKLAEQYDTFVKFTHDQLMRR
FGEQPASYVS
>Gasterosteus_aculeatus_Akirin2(1)groupXV(BT028633)
MACGATLKRTMDFDPLMSPTSPKRRRCIPVSHSSS-------------SSSPRKYLSMEP
SPFGESSSRLSAEQILNSIKQEYKRIQKRKHLDSGYQPSEGCYSPESPSQSSTMTVSGGV
SPTRKEQPLFTLRQVGMICERLLSEKEETVREEYEETMTSKLAEQYDTFVKFTHDQLMRR
FGEQPASYVS
>Sparus_aurata_Akirin2(2)(AM978490)
MACGATLKRSLDFDPLMNPASPKRRRCAPIMSP---------------VSSPQKYLRMEP
SPFGEVSSRLTAEQILHNIKQEYKRLQKRRHLDSAFQQ---ADSSCPVDLQNIYSGSGAS
SPTRKEQPLFSLRQVGMICERLLKEREDKIREEYDEILTTKLAEQYDAFVKFTHDHWMRR
FGEQPASYVS
>Gasterosteus_aculeatus_Akirin2(2)groupXVII(BT027607)   
MACGATLKRTLDFDPLMSQASPKRRRCAPIASP---------------VSSPQKYSRMEP
SPFGEVSSRLTTEQILHNIKQEYKRLQKRRHLDSGFQQ---ADSCCPLDLQNVHSGPGAS
SPTRKEQPLFSLRQVGMICERLLKEREDKIREDYDEMLTTKLAEQYDAFVKFTHDQLMRR
FGEQPASYVS
>Oryzias_latipes_Akirin2(2)chr24(Ensembl_gene_ID:ENSORLG00000011455) 
MACGVTLKRTLDFDPLMNQASPKRRRCTPVMSP---------------VSSPQKYLRMDP
SPFGEVSSRLTTEQILHNIKQEYKRLQKRRHLDSTFLQ---SDGCCPVDLQSSQCGSAAS
PPSRKEQPLFSLRQVGMICERLLKEREDKIREEYDETLTTKLAEQYDAFVKFTHDQLMRR
FGEQPASYVS
>Takifugu_rubripes_Akirin2(2)scaf94(Ensembl_gene_ID:ENSTRUG000000177990)
MACGATLKRTLDFDPLMNPASPKRRRCTPMMSP---------------VSSPQKYLRMEP
SPFGEVSSRLTTEQILHNIKQEYKRLQKRRHLDNAFQQADGCCPLDLQSIPSCLALPAAT
SPSRKEQPLFSLRQVGMICERLLKEREDKIREEYDEILTTKLAEQYDAFVKFTHDQLLRR
FEEQPASYVS