Additional file 1
(A)   Alignment of amino acid sequences of ACDs of sHsps and Acds used for generating phylogenetic tree in Figure 1.

               ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 
                   5          15         25         35         45         55                     
Hsp18.0-CI     ---------- -DWKET--PE AHVFKADVP- -GLKKEEVKV EVEDG---NV LQISGER--- 
Hsp17.4-CI     ---------- -DWKET--PE AHVFKADVP- -GLKKEEVKV EVEDG---NV LQISGER--- 
Hsp17.7-CI     ---------- -DWKET--PE VHVFKADVP- -GLKKEEVKV EVDDG---NI LQISGER--- 
Hsp17.9A-CI    ---------- -DWKET--PE AHVFKADVP- -GLKKEEVKV EVDDG---NI LQISGER--- 
Hsp16.9B-CI    ---------- -DWKET--PE SHVFKADLP- -GVKKEEVKV EVEEG---NV LVISGQR--- 
Hsp16.9A-CI    ---------- -DWKET--PE SHVFKADLP- -GVKKEEVKV EVEEG---NV LVISGQR--- 
Hsp16.9C-CI    ---------- -DWKET--PE SHVFKADLP- -GVKKEEVKV EVEEG---NV LVISGQR--- 
AtHsp17.6A-CI  ---------- -DWKET--AE AHVFKADLP- -GMKKEEVKV EIEDD---SV LKISGER--- 
Hsp16.6-CVIII  ---------- -DWKET--PT AHVFTADLP- -GVRKDQAKV EVEDG---GV LVISGER--- 
Hsp18.8-CX     ---------- -DWKET--AA AHVFMADMP- -GVRREEVRV EVEEE---KV LRISGQR--- 
Hsp17.9B-CIX   ---------- -ESRET--AE AYVFRADLPA -GVKKEEVRV EVDEG---NV LVITGER--- 
Hsp23.2-ER     ---------- -DWRET--GD AHEVVVDVP- -GMRKEDLRV EVEDN---RV LRISGER--- 
AtHsp22-ER     ---------- -DWKET--AE GHEIMLDIP- -GLKKDEVKI EVEEN---GV LRVSGER--- 
Hsp21.8-ER     ---------- -DWKET--PE AHVVTVDVP- -GVRRGDVRV EVDEAS--RV LRVSGER--- 
Hsp17.8-CXI    ---------- -DWRET--PV AHVFEMDLP- -GLAKDQVAV EVVDG---HI LRVRAGG--- 
Hsp16.0-Px     ---------- -DWVET--PT SHVLRINVP- -GLGKDDVKV QVEDG---NV LTVRGAAPHA 
At15.7Px       ---------- -DWMES--NN SHIFKINVP- -GYNKEDIKV QIEEG---NV LSIRGEG--- 
Hsp18.8-CV     ---------- ---ISL--ID TKRGMVGL-- -GVRKEEIRV EVEDA---MY LVIRT----- 
AtHsp15.4-CI(r)---------- -RWSQS--PD SHTFSVDLP- -GLRKEEIKV EIEDS---IY LIIRT----- 
AtHsp17.6-CII  ---------- -DVIEH--PN AYAFVVDMP- -GIKGDEIKV QVEND---NV LVVSG----- 
AtHsp17.7-CII  ---------- -DVIEH--PD AYVFAVDMP- -GIKGDEIQV QIENE---NV LVVSG----- 
Hsp18.0-CII    ---------- -DVKDL--PG AYAFVVDMP- -GLKSSDIKV QVEEE---RL LVISG----- 
Hsp19.0-CII    ---------- -DVKELRASG ALVLAVDMP- -GVAPADVRV EVEDG---NV LAISG----- 
Hsp18.6-CIII   ---------- -DIMET--PG EYAFVLDVP- -GLSKSDIQV TLEED---RV LVMKSSNGAG 
AtHsp17.4-CIII ---------- -DILES--PK EYIFYLDIP- -GISKSDIQV TVEEE---RT LVIKS----- 
Acd58.3        ---------- -SIYE--DDQ GYLIMVSLP- -FVDQQKVKV SWRNSLTHGI VKILCVSTAQ 
AtAcd55.8      ---------- -TVYE--DEE AYLVVITLP- -FVDLNTVKV SWRNNITNGI VKVTGLSTSR 
AtAcd39.4      ---------- -DIGE--CDD AYLFRVSLP- -GVKRDERYF SCEVE-DNGK VLVRGVTTTG 
AtAcd22.3      ---------- -DIAE--SED SYYFRVALP- -GVSRDEKEF SCEIE-PDGK IMIKGATTTG 
AtAcd15.5      ---------- -DIGV--SEV AYIFRVSLP- -GIEKNQDKI KCEIQ-REGR VCIQGVIP-E 
AtAcd28.7      ---------- -LSRE--TNS VFFLILHLK- -GFKKDGIDI EINKE--GNL IKISGRKQVE 
AtAcd216.9     ---------- ---------- ---------- ---------- ---------- ---------- 
Hsp22.3-CVI    ---------- -DWAE--TDS EYYLRADIP- -GGRKCDVEV SGDDA--MRV VDVSGLWRAA 
AtHsp21.7CI(r) ---------- -DWSQ--TDQ AYVLKSDIP- -VVGKNNVQV YVDIN--GRV MEISGQWNSN 
Acd52.9        ---------- -NVRQ--TKD CFEIYALVP- -GLLREEVHV QSDPA---GR LVITGDPE-- 
Acd50.4        ------DWVK INVRR--TKD CYEVYALVP- -GLLREEVHV QSDPA---GR LIVTGEPE-- 
AtAcd86.6      ---------- -NVQR--TQD CFEVYALVP- -GLVREEVRV QSDPA---GR LVISGEPE-- 
AtAcd48        ---------- -NVRE--TKD CFEIFALVP- -GLLREEVRV QSDPA---GR LVIAGQPE-- 
AtAcd44.3      ---------- -NVKE--SKD SFEIFALVP- -GLLRKEVRI QSDPA---GK VVITGQPE-- 
Acd29.4        ---------- -EWLD--AGA HYLLRVNVP- -EFKKEELQV HVDP---AGR LTVRGQH--G 
Acd34.9        ---------- -EWLD--NAN NFLLRLYLT- -GFKKEDFRV QVDG---TGK LTVRGQRPAA 
Acd18.1        ------DLRE QVWLDG-AAA GYVVRLDIA- -GFSKDEVDV RVNGA--TGR VTVLGQRPAA 
Acd18.0        ---------- -KMVR--EPP THTLTIDLSA KGYKKEHIKV QLVRS--RRR LVVSGECPVA 
Acd22.6        ---------- ---VE--EAG KKVLQINLSA AGFKKEQLRV QIDN---HGK LRISGERQVS 
AtAcd16.6      ---------- ---------- ---------- -GLKKEHLKI QINN---SGV LTITGGCPVD 
Acd19.4        ---------- -EWIH--GDE FDTLVLDVT- -GFSKDHLKV QVEA---SGS LRISGERAVN 
Acd41.4        ---------- -EWTR--TED ADTLVVDVS- -GFRKEELKV LYNT---SRK LKVAGERRAD 
Acd31.8        ---------- -EWSR--SAE ADAVKISLP- -GFKREEIRV -LVDN--HGH LRTRGERPVA 
AtAcd41.3      ---------- -EWKD--QPE ATILNIDLT- -GFAKEQMKV TYVHS--SKM IRVTGERPLA 
AtAcd28.1      -----EFEPA TRWTS--EPD AEVLVADLP- -GFKKEQLKV -SVTA--TRK LRLTGERPTG 
AtAcd25.1      ---------- ---------- ---LTIYLP- -GFRKEQLKV -QVTT--TRK LRVMGDRPAG 
Hsp26.7-P      ---------- -DVMED--DK EVRMRFDMP- -GLSREEVKV MVED----DA LVIRGEHK-- 
Acd19.1        ---------- -DIMDD--DK RGEIRFDML- -GLSREDVKV MVED----NM LVIRGEHS-- 
AtHsp25.3-P    ---------- -DIKEE--EH EIKMRFDMP- -GLSKEDVKI SVED----NV LVIKGEQK-- 
Hsp26.2-MI     ---------- -VAKED--DD AVHLKVSMP- -GLGKEHVKV WAEQ----NS LVIKGEGE-- 
Hsp24.0-MI     ---------- -NAKES--EE ALHLRVDMP- -GLGKEHVKV WAEQ----NS LVIKGEGE-- 
AtHSP23.6M     ---------- -DIKEK--DD ALYLRIDMP- -GLSREDVKL ALEQ----DT LVIRGEGK-- 
AtHsp23.5-M    ---------- -NVKEK--DD ALHLRIDMP- -GLSREDVKL ALEQ----NT LVIRGEGE-- 
Hsp23.6-MII    ---------- -KVRED--EE RYRLRFEVP- -GLGKDDVRV YVDD----GV LAIHGEKRDV 
AtHsp26.5-P(r) ---------- -QVKEQ--DD CYKLRYEVP- -GLTKEDVKI TVND----GI LTIKGDHK-- 
AtAcd27.7      ---------- ESKQLP--NG KLFVRADMP- -GVPKENFTV SVTN----GR VKVTGQAP-- 
AtAcd25.4      ---------- -SVKLP--NG KLFVRADMP- -GVPKENFTV SVTN----GR VKVTGEAP-- 
AtAcd81.4      ------EMAY ESKQLQ--NG GLYVRVDMP- -GVPKENFTV AVMN----GR VRVTGEAP-- 
AtAcd57.7      SVLEGPMMPY ETKQLS--NG GLYMRVDMP- -GVPSEKFMV AVDGD---GV VTIMGRAP-- 
Acd50.7        ---------- -ATSDT--DA AFLVLAHLP- -GYDKEEVEV VVGDG-GREV GVVVGARKDD 
Acd21.0        ---------- -QVAEN--KD EVSLWFDVP- -GLSPADLIV EIDED----V LVIKKKKKAS 
Acd38.4        ---------- ---------- -VQLWLQVP- -GLTEDDLEI TTTD----EL LEIKRKAG-- 
Acd21.5        ---------- --ITED--DD YVKLWFHVG- -EIDREKLKV RIEHD--TVL LVSYGGAG-- 
AtHsp14.7-P(r) ---------- -EVTET--KK SCVTRVDMP- -GCPESDLTY WVDAN---NV HFFADEP--- 
AtAcd32.1-Px   ---------- -NVAES--TH SYVVAIELP- -GASINDIRV EVDN----TN LTVTGRRTSI 
AtAcd54.2      ---------- -QWTNS--GS SIFLYVNLP- -GFYRDQIEI KKDER--TRT VQIQGQRP-- 
Acd30.2        ---------- -DIPTG--GK HVQMKFHVP- ETIDPAKLHV SIKDR----D LIVKAEDK-- 

               ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 
                   65         75         85         95        105        115                   
Hsp18.0-CI     --SKEQ---E EKTD-----K WHRVER---- SSG--KFLRR FRLP--ENTK PEQ-IKASME 
Hsp17.4-CI     --IKEQ---E EKTD-----K WHRVER---- SSG--KFLRR FRLP--ENTK PEQ-IKASME 
Hsp17.7-CI     --SREQ---E EKSD-----K WHRVER---- SSG--KFLRR FRLP--ENTK PEQ-IKASME 
Hsp17.9A-CI    --NKEQ---E EKTD-----Q WHRVER---- SSG--KFLRR FRLP--DNAK PEQ-IKASME 
Hsp16.9B-CI    --SKEK---E DKND-----K WHRVER---- SSG--QFMRR FRLP--ENAK VDQ-VKAGME 
Hsp16.9A-CI    --SKEK---E DKND-----K WHRVER---- SSG--QFMRR FRLP--ENAK VDQ-VKAGLE 
Hsp16.9C-CI    --SKEK---E DKND-----K WHRVER---- SSG--QFMRR FRLP--ENAK VDQ-VKASME 
AtHsp17.6A-CI  --HVEK---E EKQD-----T WHRVER---- SSG--GFSRK FRLP--ENVK MDQ-VKASME 
Hsp16.6-CVIII  --AREEDV-D GKNDE----R WHHVER---- SSG--KFQRR FRLP--RGAR VDQ-VSASMD 
Hsp18.8-CX     --ARAA---E EKGE-----R WHRVER---- SSE--RFVRT VRLP--PNAN TDG-VHAALD 
Hsp17.9B-CIX   --SVRR---E EKGQ-----R SHHIER---- SCA--TFFGR FHLP--DDAV VDL-VRASMD 
Hsp23.2-ER     --RREETT-E QKGG---GDH WHREER---- SYG--RFWRQ LRLP--DNAD LDS-IAASLD 
AtHsp22-ER     --KREE---E KK-----GDQ WHRVER---- SYG--KFWRQ FKLP--DNVD MES-VKAKLE 
Hsp21.8-ER     --RRAGAA-E EEEGERDGVR WHRAER---- AAG--RFWRR FRMP--PGAD VGR-VAARLD 
Hsp17.8-CXI    --EHEDANNA AKAG---KAS GEEEEE---- NDGV-RWHCR ERAAG-RRRA ADE-ASARMA 
Hsp16.0-Px     AAEKER---E REKD----VV WHVAER---- -GRP-EFARE VALP--AEVR VEQ-IRASVD 
At15.7Px       --IKE----E KKEN----LV WHVAEREAFS GGGS-EFLRR IELP--ENVK VDQ-VKAYVE 
Hsp18.8-CV     ---------E LDDG-----G DGDGGG---- GGGRRSFARK FRLP--AMVD ADG-ISAEYT 
AtHsp15.4-CI(r)---------E ATPM-----S PPDQP----- ---LKTFKRK FRLP--ESID MIG-ISAGYE 
AtHsp17.6-CII  --ERQR---E NKEN--EGVK YVRMER---- RMG--KFMRK FQLP--ENAD LDK-ISAVCH 
AtHsp17.7-CII  --KRQR---D NKEN--EGVK FVRMER---- RMG--KFMRK FQLP--DNAD LEK-ISAACN 
Hsp18.0-CII    --ERRRGGGE EEKE--ESCK YLRMER---- RMG--KFMRK FVLP--DNAD VDK-ISAVCQ 
Hsp19.0-CII    --ERRRPAGD GDDGG-EGVK YLRMER---- RMG--KFMRR FPLP--ESAD LDG-VRAEYK 
Hsp18.6-CIII   NGKRKR---E EEEGE---CK YIRLER---- RASPRAFARK FRLP--EDAD TGG-ISARCE 
AtHsp17.4-CIII NGKRKR---D DDESE-EGSK YIRLER---- RLA-QNLVKK FRLP--EDAD MAS-VTAKYQ 
Acd58.3        TPYIRRHDRV FKLAD-PMPE HCPHGE---- ------FVRE IPLAT--RIP EDAKLEAYFD 
AtAcd55.8      ASFVKRRDRT FKLVD-QMAE HCPPGE---- ------FMRE IQLPN--RIP EEANIEAYFD 
AtAcd39.4      GKRVKRYSHV FEMQ---TRS LCPPGN---- ------FSVS FRLPG--PVH PHE-FSGNFG 
AtAcd22.3      EQTVCKHNQI FKML---TQN LCPPGH---- ------FTIN FQLPG--PVS NEE-FNGNFG 
AtAcd15.5      IAIPSDTGCL YRMQ---VQQ LCPPGP---- ------FSIT FNLPG--QVD PRL-FSPNFR 
AtAcd28.7      EMVLVK---- WVEW----KK ETEIKE---- ------FKKV FRIPD--IVI LDK-IKARFN 
AtAcd216.9     ---------- --------RK EVEFSV---- ------FKKV FRIPD--TVD LDK-IKARFD 
Hsp22.3-CVI    PPPPPPDGRD WRAG-----R WWEHG----- ------FVRR VELPE--DAD WRK-VEAFFD 
AtHsp21.7CI(r) KK--AATNSD WRSG-----R WWEHG----- ------YVRR LELPS--DAD AKY-SEAFLS 
Acd52.9        ---------- -QPD-----N PWGITP---- ------FKKV VNLPL--RID PHQ-TSAVVT 
Acd50.4        ---------- -QLD-----N PWGVTP---- ------FKKV ISLPS--RID PHQ-TSAVVT 
AtAcd86.6      ---------- -NPM-----N PWGATP---- ------FKKV VSLPT--RID PHH-TSAVVT 
AtAcd48        ---------- -QLD-----N PWGITP---- ------FKKV VNFPA--RID PLH-TSAVVS 
AtAcd44.3      ---------- -QLD-----N PWGITP---- ------FKKI VDLSA--RID PLH-TSAVMS 
Acd29.4        G--------- ---------- ----LR---- ------LNKV FQLPP--TCN LDA-ITGRLE 
Acd34.9        GS-------- ---------- -KHNTR---- ------FHKV FQLPS--NAN IDD-ITGRFE 
Acd18.1        AG-------- ---------- --PHVR---- ------LRRV IQLPP--TAD SDR-VAARFV 
Acd18.0        GET------- ---------- -NRWSR---- ------FRLQ FPVPD--GCD LKA-IQARLH 
Acd22.6        G--------- ---------- -NRWSR---- ------FHKD FQVPD--DCN AGD-VRARFD 
AtAcd16.6      Q--------- ---------- -TKTIR---- ------FMKE TKVAK--DCK RNE-IRAKFS 
Acd19.4        GGG------- ---------- -RHWLH---- ------FLKR FDLPPGAADD ASA-IKVQLD 
Acd41.4        GG-------- ---------- --QWAR---- ------FLKM FPVPR--SCD AGA-IRAVMD 
Acd31.8        G--------- ---------- -NRWSR---- ------FQKD FQLPA--DCN VDG-IRAKFE 
AtAcd41.3      N--------- ---------- -RKWSR---- ------FNEV FTVPQ--NCL VDK-IHGSFK 
AtAcd28.1      G--------- ---------- -NKWIR---- ------FHQE IPVPL--TVD IDS-VSAMFK 
AtAcd25.1      A--------- ---------- -NKWIR---- ------FRKE FPIPP--NID VDS-VSAKFE 
Hsp26.7-P      KEEGE-GAEG SGDGW----W KERSVSS--- ------YDMR LALP--DECD K-SKVRAELK 
Acd19.1        KEEKERGAPT MGGG------ --RSAA---- ---------- ---------- ---------- 
AtHsp25.3-P    KEDSD----D SWSG------ --RSVSS--- ------YGTR LQLP--DNCE K-DKIKAELK 
Hsp26.2-MI     KDP---EDDA DAAP------ -PRYTR---- ---------R IELP--ADAF KMDKIKAEMK 
Hsp24.0-MI     KEA---GEDE GAAP------ -ARYSG---- ---------R IELA--PEVY RMDQIKAEMK 
AtHSP23.6M     NEEDG-GEEG ESGN------ -RRFTS---- ---------R IGLP--DKIY KIDEIKAEMK 
AtHsp23.5-M    TEE---GEDV SGDG------ -RRFTS---- ---------R IELP--EKVY KTDEIKAEMK 
Hsp23.6-MII    VEEDR-GRDG DGECW----A -AATY----- -------HAG LLLP--EDAV A-EGITAEVR 
AtHsp26.5-P(r) AEEEK-GSPE EDEYW----S -SKSYGY--- ------YNTS LSLP--DDAK V-EDIKAELK 
AtAcd27.7      ------AVSH DSSG------ --RFYSG--- ------DVAM LSTP--VDIP S-RRIKTIAK 
AtAcd25.4      ------ALSH DSSG------ --RFYSG--- ------DGAM LSTP--VDIP S-RRIKTIAK 
AtAcd81.4      ------AISH DSSG------ --RFYTG--- ------DVAM LSTP--FDIP I-RKIKIIAK 
AtAcd57.7      ------VTMH DTSG------ --RTYVA--- ------KVAN VPRG--YDG- --GRIKLVPK 
Acd50.7        AFAVEAAVVG RRLR----VA HRQVVEG--- ------FCRV FDVPP--GVE VGR-ITVGFE 
Acd21.0        PKSNYNTPTS GAIADHQEAT ADEFSGG--- -----GIYAR LLLP--AGYS REG-VQAKLT 
Acd38.4        ---------R GDPR-----R LDDVQGVG-- -----SFHLR LLLT---KEF VSSQVTAELK 
Acd21.5        ---------- DETS------ -TPANS---- ------LDVR LLLPN-KPYD TAK-VEAELT 
AtHsp14.7-P(r) ---------- -AMP-----E YENAGR---- -----KYGGS MIFNP-EAYD VKK-TKVKLI 
AtAcd32.1-Px   CQKVDAGTKA SILG----YH KQEILQG--- -----PFKVS WPLPS--NVN KDN-VSAEFM 
AtAcd54.2      ---------- --LS------ AQTKAR---- ------FSEA YRVP--DTCD MTK-LSTSFS 
Acd30.2        ---------V EKPD------ --GVSR---- ----FYYYKR TTLP--ENTD FKS-LKCNYD 

                ....|....| ....|....| ....|....| ....|
                   125        135        145        155     
Hsp18.0-CI      NG----VLTV TVPKEE-PKK PDVKSIQVT- -----
Hsp17.4-CI      NG----VLTV TVPKEE-PKK PDVKSIQIT- -----
Hsp17.7-CI      NG----VLTV TVPKEE-PKK PDVKSIQIS- -----
Hsp17.9A-CI     NG----VLTV TVPKEE-AKK PDVKSIQIS- -----
Hsp16.9B-CI     NG----VLTV TVPKAE-VKK PEVKAIEIS- -----
Hsp16.9A-CI     NG----VLTV TVPKAE-VKK PEVKAIEIS- -----
Hsp16.9C-CI     NG----VLTV TVPKAE-VKK PEVKAIEIS- -----
AtHsp17.6A-CI   NG----VLTV TVPK------ ---------- -----
Hsp16.6-CVIII   NG----VLTV TVPKEE-TKK PQLKAIPIS- -----
Hsp18.8-CX      NG----VLTI TIPKDN-DRK PHARIIPIT- -----
Hsp17.9B-CIX    GG----MLTV TVPKVV-TDK Q--PAIAAA- -----
Hsp23.2-ER      NG----VLTV RFRKLAPDQI KGPRVVGIA- -----
AtHsp22-ER      NG----VLTI NLTK------ ---------- -----
Hsp21.8-ER      DG----VLTV TVPKVPGHRG REPRVVAID- -----
Hsp17.8-CXI     DG----VLTV TVPKRKGKKR HAGNGKAAGD DKPVC
Hsp16.0-Px      NG----VLTV VVPKEPAPAR PRTRPIAVS- -----
At15.7Px        NG----VLTV VVPK------ ---------- -----
Hsp18.8-CV      HG----VLRV TVPRLHTRAR PVV------- -----
AtHsp15.4-CI(r) DG----VLTV IVPK------ ---------- -----
AtHsp17.6-CII   DG----VLKV TVQ------- ---------- -----
AtHsp17.7-CII   DG----VLKV TIPK------ ---------- -----
Hsp18.0-CII     DG----VLTV TVEKLPPPE- ---------- -----
Hsp19.0-CII     DG----VLTV TVDKKPPPEP KKPRVVEVK- -----
Hsp18.6-CIII    NG----VLTV TVKKRPPPEK KTKSVQVT-- -----
AtHsp17.4-CIII  EG----VLTV VIKK------ ---------- -----
Acd58.3         EAAA--VLEI MVPKRGNEPE EHEVRV---- -----
AtAcd55.8       GTGP--VLEI VVPK------ ---------- -----
AtAcd39.4       TDG---ILEG VVMK------ ---------- -----
AtAcd22.3       SDG---VLEG VVKK------ ---------- -----
AtAcd15.5       SDG---IFEV VVVK------ ---------- -----
AtAcd28.7       EEDG--TLTV TMPK------ ---------- -----
AtAcd216.9      DDDA--TLTI TMPK------ ---------- -----
Hsp22.3-CVI     DGE--GLLEI KVPK------ ---------- -----
AtHsp21.7CI(r) NNDDYSFLEI RIPK------ ---------- -----
Acd52.9        LHG---QLFV RAPF------ ---------- -----

Additional file 1 
(B)  Alignment of amino acid sequence of sHsp genes of rice and other plant species used for constructing phylogenetic tree in Figure 2.    

             ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 
                 5          15         25         35         45         55                     
Le26.2       MAYTSLTSSP LVSNVSVGGT SKINNNKVSA PCSVFVPSMR ---RPTTRLV -ARAT----- 
Vv25.0       --MASLKAFP FSSSPLATHK PSLSKGVAGA PCSAFFPSSR HGGRSRLALV RAEAT----- 
At25.3       --MASTLSFA ASALCSPLAP SPSVSSKSAT PFSVSFP--- ---RKIPSRI RAQDQ----- 
Hsp26.7-P    ----MAAPFA LVSRVSPAAR LPIRAAWRRA RPTVGLPSSG ---RARQLAV ASAAQ----- 
Ta26.7       -MADANAPFA LVSRLSPAAR LPIR-AWRAA RPAP-LSTGG ---RTRPLSV ASAAQ----- 
Zm26.4       ---MAAAPFA IAGRLSPVAR LPVR-AWR-- -PAHGFASSG ---RARSLAV ASAAQ----- 
At23.6-M     --MAS--ALA LK-------R LLSSS----- ----IAPRSR --SVLRP-AV SSRL------ 
At23.5-M     --MASSSALA LR-------R LLSSSTV--- ----AVPRAL --RAVRPVAA SSRL------ 
Hsp26.2-MI   --MASTVALK GRPLATLLRQ LLAADAPPAA TGRPVAAAPA --ASGKPVTA PAAATATNAA 
Hsp24.0-MI   --MASIVASK RIPLFRLVEQ LLAASP---- -----AQGAA --SALRPVAV AGGS------ 
Zm22.8       ---MALARLC LNRAIAGRAQ ALARPALAAA PATDPKLHS- -------LLS TSAADSAAS- 
Hsp23.6-MII  ---MALARQC LSKRLAA-GC ALARPLHAAS PVAAAAANSH GPLNFRALFS SAGADAAATT 
At26.5       ---MALARLA LRNLQQKL-- --SPSLMGQS -CERGLVGNR HNPMKLNRFM AT--SAGEQ- 
Vv26.3       ---MALARLA LRSLQQRVPS SSSSSLLSPS LSERALTGQR WGPEIVKRFS ATPDAASDK- 
So17.4       ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Sb17.4       ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Zm17.4       ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Cd16.6       ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Hsp17.9B-CIX ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Zm16.6       ---------- ---------- ---------- ---------- ----MSPA-- ---------- 
So16.5       ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Hsp16.6-CVIII---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Hsp16.9B-CI  ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Hsp16.9A-CI  ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Hsp16.9C-CI  ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Zm17.2       ---------- ---------- ---------- ---------- ----MSLV-- ---------- 
Ta16.9B      ---------- ---------- ---------- ---------- ----MSIV-- ---------- 
Hsp17.9A-CI  ---------- ---------- ---------- ---------- ----MSLI-- ---------- 
Hsp18.0-CI   ---------- ---------- ---------- ---------- ----MSLI-- ---------- 
Hsp17.4-CI   ---------- ---------- ---------- ---------- ----MSMI-- ---------- 
Hsp17.7-CI   ---------- ---------- ---------- ---------- ----MSLI-- ---------- 
Zm17.9       ---------- ---------- ---------- ---------- ----MSLI-- ---------- 
At17.4CI     ---------- ---------- ---------- ---------- ----MSLVP- ---------- 
Ta20.1       ---------- ---------- ---------- ---------- ----MSMISS MLGRKQPPPQ 
Hv19.3       ---------- ---------- ---------- ---------- ----MSMISN MLGRRQPPP- 
Ps19.8       ---------- ---------- ---------- ---------- ----MSMISS MLGRRQPPAQ 
Hsp18.8-CX   ---------- ---------- ---------- ---------- ----MSMITS MLGRK----- 
Vv16.4       ---------- ---------- ---------- ---------- ----MSMLS- ---------- 
Pt17.6       ---------- ---------- ---------- ---------- ----MSLLHS ---------- 
Hsp17.8-CXI  ---------- ---------- ---------- ---------- ----MSLVLS ---------- 
Zm22.7       ---------- ---------- ---------- MADAVLFSRK ASASAAVVVL AVAVVTMACL 
Hsp21.8-ER   ---------- ---------- ---------- MA-------- AVAEREVLGM VAAVAAMVVM 
Vv21.3       ---------- ---------- ---------- ---------- -MAKPSIIPI SLFLAAMAAV 
At22         ---------- ---------- ---------- ---------- ---MMKHLLS IFFIGALLLG 
Hsp23.2-ER   ---------- ---------- ---------- ---------- -MASMRTAAA AAMLACIAVV 
Hv17.1       ---------- ---------- --------MD ---------- ---------- ---------- 
Hsp18.8-CV   ---------- ---------- --------MD YY-------- ---------- ---------- 
Zm17.2       ---------- ---------- --------MD YS-------- ---------- ---------- 
Vv15.7       ---------- ---------- --------MD ---------- ---------- ---------- 
At15.4       ---------- ---------- --------MD ---------- ---------- ---------- 
Lp16.1CIII   ---------- ---------- --------MS T--------- ---------- ---------- 
At17.4CIII   ---------- ---------- --------MS ---------- ---------- ---------- 
Hsp18.6-CIII ---------- ---------- --------MT EL-------- ---------- ---------- 
At17.6-CII   ---------- ---------- --------MD LG-------- ---------- ---------- 
At17.7       ---------- ---------- ------MDLE FG-------- ---------- ---------- 
Hsp18.0-CII  ---------- ---------- --------ME SA-------- ---------- ---------- 
Hsp19.0-CII  ---------- ---------- --------ME LS-------- ---------- ---------- 
At15.7       ---------- ---------- ---------- ---------- ---------- ---------- 
Gm16.2       ---------- ---------- ---------- ---------- ---------- ---------- 
Hsp16.0-Px   ---------- ---------- ---------- ---------- ---------- ---------- 
Vv22.4       ---------- MTSSK---QL EVQSEDR--- TPQKWCVSLR EDKFEAFLSQ GN-------- 
At21.7       ---------- MTSSSGSLKL EIHTDDK--- TPGKWSVPLG DDVFRRFLSG GG-------- 
Hsp22.3-CVI  ---------- MPPRRG-IEV RQAVGDG--- AAPRWRMSLL ENTFSSFLQS IGGGVAADG- 
Zm21.9       ---------- MPGRRA-IDV RLLPGGGDAA AAPKWRMSLL ENTFSGFLQG AG----AD-- 
Clustal Co                                                                     

             ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 
                 65         75         85         95        105        115                   
Le26.2       -----GDNKD TSVDVHHSSA QGGNN--QGT AVERRPT-RM ALD------- -VSPFGVLDP 
Vv25.0       -----GENKD ASLDVQ---V HQGNK--GAT AVERQPR-RL ALD------- -ISPFGLLDP 
At25.3       -----REN-- -SIDVVQQGQ QKGN---QGS SVEKRPQQRL TMD------- -VSPFGLLDP 
Hsp26.7-P    -----ENRDN TAVDVHVN-- QDGGN-QQGN AVQRRPRRSS ALDG------ -ISPFGLVDP 
Ta26.7       -----ENRDN -SVDVQVSQA QNAGN-QQGN AVQRRPRR-A GFD------- -ISPFGLVDP 
Zm26.4       -----ENRDN -SVDVQVS-- QNGGNRQQGN AVQRRPRRAT ALDI------ SPSPFGLVDP 
At23.6-M     -----FNTN- -AVRSYDD-- --DGENGDGV DLYRRSVPRR RGDF------ FS--DVF-DP 
At23.5-M     -----FNTN- -AARNYED-- --GVDRN--- HHSNRHVSRH GGDF------ FS--HIL-DP 
Hsp26.2-MI   SR-RLYNTEG APLRRYDVVD ESGTDSGDEY DATDDGRRLT VPFF------ FSASDVL-DP 
Hsp24.0-MI   ---RAYNTG- AQLRRHER-D ESDDDSGRGY DTRRPTRDAT MPAF------ FS--DVFRDP 
Zm22.8       -------GEA NRRDVAVSER SAPNRRWAWR DLRD------ ---------- FTPFS---LV 
Hsp23.6-MII  GGCAPAKGDG HSREVAVVDR SR--RRWPWR DLRD------ ---------- FVPLR---LV 
At26.5       -------EDK MNTEVSVSEK KSPRQNFPRR RGRKSLWRNT DDHGY----- FTPTLNEFFP 
Vv26.3       -------PSG EKTEVAVSEG DRKPKLFPRK QRKRSLWRNN RND------- FVPSLNELFP 
So17.4       ---------- ---------- --RLMDTLAL DSWVRNPFS- ---------I FGTAVAA-DA 
Sb17.4       ---------- ---------- --RLLDTLAL DSWVRNPFS- ---------I FGTAVAA-DA 
Zm17.4       ---------- ---------- --RLLDTLAL DSWVRNPFT- ---------I FGTAVAA-DA 
Cd16.6       ---------- ---------- --RLFDTLAL DTWK-NPFS- ---------I FGTPLSA-DA 
Hsp17.9B-CIX ---------- ---------- --KLFDTLAF DAWN--PFS- ---------I FGTTVAA-DA 
Zm16.6       ---------- ---------S SS---NPLSL DFWASSADP- ---------- FGVVRP---- 
So16.5       ---------- ---------R SSNVFDPLSL DFWTS-ADP- ---------- FGVVRP---- 
Hsp16.6-CVIII---------- ---------R SGNVLDPMSV DFWAD-ADP- ---------- FGAVRS---- 
Hsp16.9B-CI  ---------- ---------R RSNVFDPFSL DLWDPFDS-- ---------V FRSVVP--AT 
Hsp16.9A-CI  ---------- ---------R RSNVFDPFSL DLWDPFDS-- ---------V FRSVVP--AT 
Hsp16.9C-CI  ---------- ---------R RSNVFDPFA- DFWDPFDG-- ---------V FRSLVP--AT 
Zm17.2       ---------- ---------R RSNVFDPFSM DLWDPFDT-- ---------M FRSIVPS-AT 
Ta16.9B      ---------- ---------R RTNVFDPFAD LWADPFD--- ---------T FRSIVPA-IS 
Hsp17.9A-CI  ---------- ---------R RSNVFDPFSL DLWDPFDG-F PFGSGGSS-- SGSIFPSFPR 
Hsp18.0-CI   ---------- ---------R RSNVFDPFSL DLWDPFDG-F PFGSGSRS-- SGSIFPSFPR 
Hsp17.4-CI   ---------- ---------R RSNVFDPFSL DLWDPFDG-F PFGSG----- SGSLFP---R 
Hsp17.7-CI   ---------- ---------R RGNAFDPFSL DLWDPVDG-F PFGSGGSSSS SGSLFP---R 
Zm17.9       ---------- ---------R RGNAFDPFSL DLWDPFEGFF PFGSGGVR-- --SLVPSLPR 
At17.4CI     ---------- ----SFFGGR RTNVFDPFSL DVWDPFEG-- ---------- --FLTPGLTN 
Ta20.1       PQPQQHAAGH RANGHGHGGG GDEVMEPVSI DILEPFMEAI S--------- LTAFGGG--G 
Hv19.3       PH-QQQPAGH KTN--GHGGG G-EVVEPVSI DILEPFMEAI S--------- LKAFGG---- 
Ps19.8       QQPQQQAADH KAN--GHGGG GGEAVEPVSI DILEPFMEAI S--------- LTAFGAG--G 
Hsp18.8-CX   QNAQQKGGGG GGR---TGGG GGGEIEPVSV DIMEPFMDAI S--------- LTAFAAAPSA 
Vv16.4       ---------- ---------- ---------- SLFENLG--- ---------- ---------- 
Pt17.6       ---------- ---------- -----LLNQN SLFDPFRGF- ---------- ---------- 
Hsp17.8-CXI  ---------- ---------- -----RMLLD RFFPGAGGV- ---------- ---------- 
Zm22.7       AAP--VAALV PYGRPGG--- ----GLWDLM LLDDPFRVLE QS-------P LAASSVPRAS 
Hsp21.8-ER   MAPP-AAALV PYGYG----- ---------Y MLDDPFRVLE QS-------P LRPAGGVAAA 
Vv21.3       LIPIPAEGLM PYTRN----- ----LWDMVL PFDDPFRILE HS-------P ITVPKGLET- 
At22         NIKTS-EGSL SSALET--TP GSLLSDLWLD RFPDPFKILE RI-------P LGLERDTSVA 
Hsp23.2-ER   LASTAADGAL LPWFGGGGAR DEAVPELGLL AAADPFRILE HV-------P FGFDRDDVAM 
Hv17.1       ---------- ---------- -YPMQ---EV QQHPGFR-AV HPWQWWQWQ- -LSVLASSSS 
Hsp18.8-CV   ---------- ---------- -YPMEEEEEV HERPRFRRPV HPWQWHHWQN LLGLLSSSSP 
Zm17.2       ---------- ---------- -YPME----- EQHSGFR--- -QWQW-QWR- -LFSLLSSQP 
Vv15.7       ---------- ---------- ---------- -----FS--- -PFQPSSWH- --FLFTS--- 
At15.4       ---------- ---------- ---------- -----FQ--- -TIQVMPWE- --YVLAS--- 
Lp16.1CIII   ---------- ---------- --VVDVVSQL LFPESIERLV SP-----SRS ----NESKG- 
At17.4CIII   ---------- ---------- --AVAINHFF GLPEAIEKLI LPI----SRS GESNNESRGR 
Hsp18.6-CIII ---------- ---------- -FDTAVTSLL HLPEVLDRLG AAAGDRRSAG DHAHHAAHGH 
At17.6-CII   ---------- ---------- -RF----PII SILEDMLEVP EDHN----NE KTRNNPSRVY 
At17.7       ---------- ---------- -RF----PIF SILEDMLEAP EEQ-----TE KTRNNPSRAY 
Hsp18.0-CII  ---------- ---------- -MFGLETPLM TALQHLLDIP DGEGGAAGKQ GATGGPTRAY 
Hsp19.0-CII  ---------- ---------- -VVGGEPLLA VAMQQLLDLD LPDE----LE RQLNPPTRAY 
At15.7       ---------- ---------- -----MADRG IFLYPFRR-- ---------- ---------- 
Gm16.2       ---------- ---------- -----MAGT- IFGYPFRHFI WGH------- --------PP 
Hsp16.0-Px   ---------- ---------- -----MADL- FFGGPFRRIL YG-------- --------RP 
Vv22.4       ---------- ---------- PTVRKVFGDG SLFSPFLFRK FFDPS---DA FPLWEFESDV 
At21.7       ---------- ---------- GSEKAVFGEG SLFSPFLFGK YFDPS---DA FPLWEFEAEV 
Hsp22.3-CVI  ---------- ---------- AAARAVFGEG SLFSPFLFGK FFDPA---DA FPLWEFEPEV 
Zm21.9       ---------- ---------- TAARAVFAEG SLFCPFLFGN FFDPA---DP FPLWEFESDV 
Clustal Co                                                                     

             ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 
                125        135        145        155        165        175               
Le26.2       MSPMRTMRQM IDTMDRLFED -TMTFPGRNR A---SGTGEI RTPWDIHDDE NEIKMRFDMP 
Vv25.0       FSPMRTMRQM MDAMDRMFEE -TVAFPG--- ------SAEV RSPWDIVDDE NEIKMRFDMP 
At25.3       LSPMRTMRQM LDTMDRMFED -TMPVSGRNR GG--SGVSEI RAPWDIKEEE HEIKMRFDMP 
Hsp26.7-P    MSPMRTMRQM LDTMDRIFDD VALGFPATPR RSLATG--EV RMPWDVMEDD KEVRMRFDMP 
Ta26.7       MSPMRTMRQM LDTMDRLFDD -AVGFP-TAR RSLAAASEMP RMPWDIMEDD KEVKMRFDMP 
Zm26.4       MSPMRTMRQM LDTMDRLFDD -AVGFPMGTR RSPATTG-DV RLPWDIVEDE KEVKMRIDMP 
At23.6-M     FSPTRSVSQV LNLMDQFMEN ---PLLSA-T RGMGASGARR G--WDIKEKD DALYLRIDMP 
At23.5-M     FTPTRSLSQM LNFMDQVSEI ---PLVSA-T RGMGASGVRR G--WNVKEKD DALHLRIDMP 
Hsp26.2-MI   FGAPTSLGRL LALMEDAAVA ---TAAAPGT NGLATAAARR GGWWVAKEDD DAVHLKVSMP 
Hsp24.0-MI   FSAPQSLGRL LSLMDDLATP ---AGRAG-- ----AATLRR G--WNAKESE EALHLRVDMP 
Zm22.8       DGLGSALSQV AETLGRPLER ---------- ----L-APSR LLSGKVREDE ARYRLRFEVP 
Hsp23.6-MII  DGIGSALSQV AETLTRP--- ---------- ---------- -LTGKVREDE ERYRLRFEVP 
At26.5       PTIGNTLIQA TENMNRIFDN ---------- ----FNVNPF QLMGQVKEQD DCYKLRYEVP 
Vv26.3       PSIGNALMQA TQHMNRLLEN ---------- ----L--APS RLIGRLKEQD QCYKLRYEMP 
So17.4       WLASDTSAFA NTYIESRDT- ---------- ---------- ---------A GAYVFSAALP 
Sb17.4       WLASDTSAFA NTYIESRDT- ---------- ---------- ---------A GAYVFSAALP 
Zm17.4       WLASDTSAFA NTHIESRDT- ---------- ---------- ---------A AAYVFSAALP 
Cd16.6       WLASDTSAFA NTYIESRET- ---------- ---------- ---------A SAYVFSAALP 
Hsp17.9B-CIX WLASDTSAFA NTYIESRET- ---------- ---------- ---------A EAYVFRADLP 
Zm16.6       -LAEQCPVLT NVRVDWKET- ---------- ---------- ---------P EAHVFRADLP 
So16.5       -LAEQCPVLT NVRVDWKET- ---------- ---------- ---------P EAHVFRADLP 
Hsp16.6-CVIII-LAERCPVLT NVRVDWKET- ---------- ---------- ---------P TAHVFTADLP 
Hsp16.9B-CI  SDN-DTAAFA NARIDWKET- ---------- ---------- ---------P ESHVFKADLP 
Hsp16.9A-CI  SDN-DTAAFA NARIDWKET- ---------- ---------- ---------P ESHVFKADLP 
Hsp16.9C-CI  SDR-DTAAFA NARVDWKET- ---------- ---------- ---------P ESHVFKADLP 
Zm17.2       STNSETAAFA SARIDWKET- ---------- ---------- ---------P EAHVFKADLP 
Ta16.9B      GGGSETAAFA NARMDWKET- ---------- ---------- ---------P EAHVFKADLP 
Hsp17.9A-CI  GASSETAAFA GARIDWKET- ---------- ---------- ---------P EAHVFKADVP 
Hsp18.0-CI   GTSSETAAFA GARIDWKET- ---------- ---------- ---------P EAHVFKADVP 
Hsp17.4-CI   -ANSDAAAFA GARIDWKET- ---------- ---------- ---------P EAHVFKADVP 
Hsp17.7-CI   -ANSDAAAFA GARIDWKET- ---------- ---------- ---------P EVHVFKADVP 
Zm17.9       -TSSETAAFA GARIDWKET- ---------- ---------- ---------P EAHVFKADVP 
At17.4CI     APAKDVAAFT NAKVDWRET- ---------- ---------- ---------P EAHVFKADVP 
Ta20.1       CRPALGLPFS TASMDWKET- ---------- ---------- ---------P TAHVFMADVP 
Hv19.3       --PALGLPFS TASMDWKET- ---------- ---------- ---------P TAHVFMADVP 
Ps19.8       -GPALGMPFS TASMDWKET- ---------- ---------- ---------P TAHVFMADVP 
Hsp18.8-CX   AAAAAGVP-S TASMDWKET- ---------- ---------- ---------A AAHVFMADMP 
Vv16.4       -----IASSG YVHMDWKET- ---------- ---------- ---------P QAHIFQVDLP 
Pt17.6       -----LIENS ETQMDWKET- ---------- ---------- ---------P HAHVFEIDLP 
Hsp17.8-CXI  -----VAGEA RPPMDWRET- ---------- ---------- ---------P VAHVFEMDLP 
Zm22.7       LDSTSAAGVA LARCDWKET- ---------- ---------- ---------P DAHVISVDVP 
Hsp21.8-ER   AAAGEPAAVA LARCDWKET- ---------- ---------- ---------P EAHVVTVDVP 
Vv21.3       --------IA LARSDWKET- ---------- ---------- ---------T SAHIITLDVP 
At22         --------LS PARVDWKET- ---------- ---------- ---------A EGHEIMLDIP 
Hsp23.2-ER   --------LS MARVDWRET- ---------- ---------- ---------G DAHEVVVDVP 
Hv17.1       PPAPSTRPP- -NHVSWDET- ---------- ---------- ---------G AAHIYSADLP 
Hsp18.8-CV   SPATAAAAQR CSHVSWEET- ---------- ---------- ---------A AAHLYSASLP 
Zm17.2       ELLPPPRPA- -NHVRWEET- ---------- ---------- ---------A AAHLFSASLP 
Vv15.7       PLLVSYHFTP DNYVHWTET- ---------- ---------- ---------P ESHIYSANLP 
At15.4       QSLNNYQ--- ENHVRWSQS- ---------- ---------- ---------P DSHTFSVDLP 
Lp16.1CIII   ---------- TIPVDILDT- ---------- ---------- ---------P KEYIFYMDVP 
At17.4CIII   ------GSSN NIPIDILES- ---------- ---------- ---------P KEYIFYLDIP 
Hsp18.6-CIII GQHRISGIGG GAPVDIMET- ---------- ---------- ---------P GEYAFVLDVP 
At17.6-CII   MRDA--KAMA ATPADVIEH- ---------- ---------- ---------P NAYAFVVDMP 
At17.7       MRDA--KAMA ATPADVIEH- ---------- ---------- ---------P DAYVFAVDMP 
Hsp18.0-CII  VRDA--RAMA ATPADVKDL- ---------- ---------- ---------P GAYAFVVDMP 
Hsp19.0-CII  VRDR--RAMA NTPMDVKELR ---------- ---------- --------AS GALVLAVDMP 
At15.7       -FQEWSRS-- TALIDWMES- ---------- ---------- ---------N NSHIFKINVP 
Gm16.2       IFKEWSGS-- TALLDWLES- ---------- ---------- ---------P TAHILKINVP 
Hsp16.0-Px   FPPDWASASA TAAMDWVET- ---------- ---------- ---------P TSHVLRINVP 
Vv22.4       LLSHLRSS-G QTTVDWLQT- ---------- ---------- ---------D KDYVLKAELP 
At21.7       LLASLRSL-G QCRVDWSQT- ---------- ---------- ---------D QAYVLKSDIP 
Hsp22.3-CVI  LLAALRRG-A RTTVDWAET- ---------- ---------- ---------D SEYYLRADIP 
Zm21.9       LLAALRRGNA RTTVDWAET- ---------- ---------- ---------D CEYYLRADVP 
Clustal Co                                                                  :* 

             ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 
                185        195        205        215        225        235               
Le26.2       -GLSKEDVKV SVEND--MLV IK-------- ------GEHK ------KEED GR-------- 
Vv25.0       -GLSKEDVKV SVEDD--LLV IK-------- ------GEQK ------KEEG EK-------- 
At25.3       -GLSKEDVKI SVEDN--VLV IK-------- ------GEQK ------KEDS D--------- 
Hsp26.7-P    -GLSREEVKV MVEDD--ALV IR-------- ------GEHK ------KEEG EG---AEGSG 
Ta26.7       -GLSREEVKV MVEGD--ALV IR-------- ------GEHK ------KEAG EGQGEAAEGG 
Zm26.4       -GLARDEVKV MVEDD--TLV IR-------- ------GEHK ------KEEG AEGG-SGGDG 
At23.6-M     -GLSREDVKL ALEQD--TLV IR-------- ------GEGK ------NEED G--------- 
At23.5-M     -GLSREDVKL ALEQN--TLV IR-------- ------GEGE ------TEE- ---------- 
Hsp26.2-MI   -GLGKEHVKV WAEQN--SLV IK-------- ------GEGE ------KDP- ---------- 
Hsp24.0-MI   -GLGKEHVKV WAEQN--SLV IK-------- ------GEGE ------KEA- ---------- 
Zm22.8       -GLGKGDVRV AVEDG--VLV IE-------- ------GEKR EH----GEEG DG-------- 
Hsp23.6-MII  -GLGKDDVRV YVDDG--VLA IH-------- ------GEKR DV----VEED RGRD----GD 
At26.5       -GLTKEDVKI TVNDG--ILT IK-------- ------GDHK ------AEEE KGSP----EE 
Vv26.3       -GLTKEDVKI SVEDG--ILS IR-------- ------GEHK ------EEEE EGS------D 
So17.4       PGVKKEEVTV EVDEG-NVLV IT-------- ------GERS V-----SREE RVG-DRWHHV 
Sb17.4       PGVRKEEVTV EVDEG-NVLV IT-------- ------GQRS V-----SREE RVG-DRWHHV 
Zm17.4       PGVKKEEVTV ELDEG-NVLV IA-------- ------GERS V-----CRQE RS--DGCHHI 
Cd16.6       PGVKKEEVRV EVDED-NVLE IT-------- ------GERS V-----RREE KG--DKWHHI 
Hsp17.9B-CIX AGVKKEEVRV EVDEG-NVLV IT-------- ------GERS V-----RREE KG--QRSHHI 
Zm16.6       -GVRKEAAKV EVEDG-NVLV IS-------- ------GERA RE---EEEAG KDEAWRWRLV 
So16.5       -GVKKEAAKV EVEDG-NVLV IS-------- ------GERA R-----EEAG KDE--KWRLV 
Hsp16.6-CVIII-GVRKDQAKV EVEDG-GVLV IS-------- ------GERA RE---EDVDG KNDE-RWHHV 
Hsp16.9B-CI  -GVKKEEVKV EVEEG-NVLV IS-------- ------GQRS K-----EKED KND--KWHRV 
Hsp16.9A-CI  -GVKKEEVKV EVEEG-NVLV IS-------- ------GQRS K-----EKED KND--KWHRV 
Hsp16.9C-CI  -GVKKEEVKV EVEEG-NVLV IS-------- ------GQRS K-----EKED KND--KWHRV 
Zm17.2       -GVKKEEVKV EVEDG-NVLV IS-------- ------GQRS R-----EKED KDD--KWHRV 
Ta16.9B      -GVKKEEVKV EVEDG-NVLV VS-------- ------GERT K-----EKED KND--KWHRV 
Hsp17.9A-CI  -GLKKEEVKV EVDDG-NILQ IS-------- ------GERN K-----EQEE KTD--QWHRV 
Hsp18.0-CI   -GLKKEEVKV EVEDG-NVLQ IS-------- ------GERS K-----EQEE KTD--KWHRV 
Hsp17.4-CI   -GLKKEEVKV EVEDG-NVLQ IS-------- ------GERI K-----EQEE KTD--KWHRV 
Hsp17.7-CI   -GLKKEEVKV EVDDG-NILQ IS-------- ------GERS R-----EQEE KSD--KWHRV 
Zm17.9       -GLRKEEVKV EVEDG-NVLQ IS-------- ------GERN K-----EHEE KND--RWHRV 
At17.4CI     -GLKKEEVKV EVEDG-NILQ IS-------- ------GERS S-----ENEE KSD--TWHRV 
Ta20.1       -GLRREEVKV EVEQE-RVLR IS-------- ------GQRA R-----AAED KGD--RWHRV 
Hv19.3       -GLRREEVKV EVEQE-RVLR IS-------- ------GQRA R-----AAED KGD--RWHRV 
Ps19.8       -GLRREEVKV EVEQE-RVLR IS-------- ------GQRA R-----RAED KGD--RWHRV 
Hsp18.8-CX   -GVRREEVRV EVEEE-KVLR IS-------- ------GQRA R-----AAEE KGE--RWHRV 
Vv16.4       -GLTKNEVKL EVHQG-RVLH I--------- ------SGC- RE---EEPEE KGE--KWHCR 
Pt17.6       -GLTKEDVKI EVHEG-TVLQ I--------- ------STAE RK---EEAEE KGD--KWHCK 
Hsp17.8-CXI  -GLAKDQVAV EVVDG-HILR VRAGGEHEDA NNAAKAGKAS GE---EEEEN DGV--RWHCR 
Zm22.7       -GVRREDVKV EVEENSRVLR VS-------- ------GERR ---ADEEKEG DR----WHXA 
Hsp21.8-ER   -GVRRGDVRV EVDEASRVLR VS-------- ------GERR RAGAAEEEEG ERDGVRWHRA 
Vv21.3       -GMKKEDIKI EIEEN-RVLR IS-------- ------GERT ---AEGEAEG EK----WHRS 
At22         -GLKKDEVKI EVEEN-GVLR VS-------- ------GERK ---REE--EK KG--DQWHRV 
Hsp23.2-ER   -GMRKEDLRV EVEDN-RVLR IS-------- ------GERR ---REETTEQ KGGGDHWHRE 
Hv17.1       -GVKKEEIRV EIEDG-RYLV VRT------- ---------- ------ELDA G---DAEVHG 
Hsp18.8-CV   -GVRKEEIRV EVEDA-MYLV IRT------- ---------- ------ELDD GGDGDGGGGG 
Zm17.2       -GVRKEEIRV EVEDA-RYLV IRT------- ---------- ------ELDA G-TGTGGAVA 
Vv15.7       -GVRKEEIRV ELEDS-RYLI IRT------- ---------- ------EAID ES------TK 
At15.4       -GLRKEEIKV EIEDS-IYLI IRT------- ---------- ------EATP MSP----PDQ 
Lp16.1CIII   -GLSKSDLQV SVEDE-KTLV IRS------- -----NGKRK R-----EESE -EEGCKYVRL 
At17.4CIII   -GISKSDIQV TVEEE-RTLV IKS------- -----NGKRK R-----DDDE SEEGSKYIRL 
Hsp18.6-CIII -GLSKSDIQV TLEED-RVLV MKSSNG---- ---AGNGKRK R-----EEEE --GECKYIRL 
At17.6-CII   -GIKGDEIKV QVEND-NVLV VSG------- -------ERQ R-----ENKE N-EGVKYVRM 
At17.7       -GIKGDEIQV QIENE-NVLV VSG------- -------KRQ R-----DNKE N-EGVKFVRM 
Hsp18.0-CII  -GLKSSDIKV QVEEE-RLLV ISG------- -------ERR RG--GGEEEK E-ESCKYLRM 
Hsp19.0-CII  -GVAPADVRV EVEDG-NVLA ISG------- -------ERR RP--AGDGDD GGEGVKYLRM 
At15.7       -GYNKEDIKV QIEEG-NVLS IR-------- ------GEG- ---IKEE--- KKENLVWHVA 
Gm16.2       -GFSKEDIKV QIEDG-NILH IK-------- ------GEV- ---WREEPQA KEKDTVWHVA 
Hsp16.0-Px   -GLGKDDVKV QVEDG-NVLT VR-------- ------GAAP HA-AAEKERE REKDVVWHVA 
Vv22.4       -GVGKNSVQV YVE-NGKVVE ISG------- -----LWRHQ K------EPK T-KEWRSGHW 
At21.7       -VVGKNNVQV YVDINGRVME ISG------- -----QWNSN K------KAA TNSDWRSGRW 
Hsp22.3-CVI  -GGRKCDVEV SGDDAMRVVD VSG------- -----LWRAA PP----PPPP DGRDWRAGRW 
Zm21.9       -GGRRCDVEV SG-DAMKVID ICG------- -----LWRA- -------PAA DGRDWRCGRW 
Clustal Co            :         :  :                                           

             ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 
                245        255        265        275        285        295               
Le26.2       DKHSWGRNYS SYDTRLSLPD NVVK-DK-IK AELKN----G VLFISIPKTE VE-------- 
Vv25.0       DSWS-GSGFS SYSTRLQLPD NCEK-DK-IK AELKN----G VLSISIPKTK VE-------- 
At25.3       DSWS-GRSVS SYGTRLQLPD NCEK-DK-IK AELKN----G VLFITIPKTK VE-------- 
Hsp26.7-P    DGWWKERSVS SYDMRLALPD ECDK-SK-VR AELKN----G VLLVTVPKTE VE-------- 
Ta26.7       DGWWKERSVS SYDMRLALPD ECDK-SQ-VR AELKN----G VLLVSVPKRE TE-------- 
Zm26.4       DGWWKQRSVS SYDMRLALPD ECDK-SK-VR AELKN----G VLLVTVPKTE VE-------- 
At23.6-M     -GEEGESGNR RFTSRIGLPD KIYKIDE-IK AEMKN----G VLKVVIPKMK EQ-------- 
At23.5-M     -GEDVSGDGR RFTSRIELPE KVYKTDE-IK AEMKN----G VLKVVIPKIK ED-------- 
Hsp26.2-MI   -EDDADAAPP RYTRRIELPA DAFKMDK-IK AEMKN----G VLRVAVPKLK EE-------- 
Hsp24.0-MI   -GEDEGAAPA RYSGRIELAP EVYRMDQ-IK AEMKN----G VLKVVVPKVK EE-------- 
Zm22.8       GEWWSTSGY- --HASLLLPD DARA-EG-IT AEVKD----G VLYVTVPRTG -E-------- 
Hsp23.6-MII  GECWAAATY- --HAGLLLPE DAVA-EG-IT AEVRD----G VLHVTVPRSP -E-------- 
At26.5       DEYWSSKSYG YYNTSLSLPD DAKV-ED-IK AELKN----G VLNLVIPRTE -K-------- 
Vv26.3       DEHWSATSYG YYDTSLLLPT DAKI-EE-IK AELKD----G VLTIIIPRNE -K-------- 
So17.4       ERCC-----A SFLGRFHLPD DAAVNG--VR AAMDA----G MLTVTVPKVG ASR--DCGGG 
Sb17.4       ERCC-----A SFLGRFHLPE DAAVDG--VR AAMDA----G MLTVTVPKVG AA----AAAI 
Zm17.4       ERSR-----A TFLARFHLPE DAAVDG--VR AALDA----G RLTVTVPKVG AA---AAAIV 
Cd16.6       ERSS-----A TFLGRFHLPE DAVVDG--VR AAMDG----G MLTVTVPKVG AA-------- 
Hsp17.9B-CIX ERSC-----A TFFGRFHLPD DAVVDL--VR ASMDG----G MLTVTVPKVV TDKQPAIAAA 
Zm16.6       ERSS-----G RFQRRFRLPR GARLDQ--VH ASMEN----G VLTVTVPKEE AK-------- 
So16.5       ERSC-----G RFQRRFRLPR GAKLDQ--VR ASMDN----G VLTVTVPKEE VK-------- 
Hsp16.6-CVIIIERSS-----G KFQRRFRLPR GARVDQ--VS ASMDN----G VLTVTVPKEE TK-------- 
Hsp16.9B-CI  ERSS-----G QFMRRFRLPE NAKVDQ--VK AGMEN----G VLTVTVPKAE VK-------- 
Hsp16.9A-CI  ERSS-----G QFMRRFRLPE NAKVDQ--VK AGLEN----G VLTVTVPKAE VK-------- 
Hsp16.9C-CI  ERSS-----G QFMRRFRLPE NAKVDQ--VK ASMEN----G VLTVTVPKAE VK-------- 
Zm17.2       ERSS-----G QFIRRFRLPD DAKVDQ--VK AGLEN----G VLTVTVPKAE EK-------- 
Ta16.9B      ERSS-----G KFVRRFRLLE DAKVEE--VK AGLEN----G VLTVTVPKAE VK-------- 
Hsp17.9A-CI  ERSS-----G KFLRRFRLPD NAKPEQ--IK ASMEN----G VLTVTVPKEE AK-------- 
Hsp18.0-CI   ERSS-----G KFLRRFRLPE NTKPEQ--IK ASMEN----G VLTVTVPKEE PK-------- 
Hsp17.4-CI   ERSS-----G KFLRRFRLPE NTKPEQ--IK ASMEN----G VLTVTVPKEE PK-------- 
Hsp17.7-CI   ERSS-----G KFLRRFRLPE NTKPEQ--IK ASMEN----G VLTVTVPKEE PK-------- 
Zm17.9       ERSS-----G KFLRRFRLPD NAKADQ--IK ASMEN----G VLTVTVPKEE AK-------- 
At17.4CI     ERSS-----G KFMRRFRLPE NAKVEE--VK ASMEN----G VLSVTVPKVQ ES-------- 
Ta20.1       ERSA-----E KFVRTVRLPP NADVDGGGVH AALDN----G VLTITIPKDD GK-------- 
Hv19.3       ERSA-----E KFVRTVRLPP NADVDGGGVH AALDN----G VLTITIPKDD GK-------- 
Ps19.8       ERSA-----E KFVRTVRLPP NADVDGGGVH AALDN----G VLTITIPKDD GK-------- 
Hsp18.8-CX   ERSS-----E RFVRTVRLPP NANTDG--VH AALDN----G VLTITIPKDN DR-------- 
Vv16.4       ERSC-----G SFSRQFRLPE DAKVEE--IK ASMHD----G VLIVTVPKDE AL-------- 
Pt17.6       ERSR-----G GFSRRFRLPE NAKLDE--IK ASMHD----G VLVVTVPKDE LK-------- 
Hsp17.8-CXI  ERAA-----G ---------R RRAADE--AS ARMAD----G VLTVTVPKRK GK-------- 
Zm22.7       ERAA-----G RFWRRFRMPA GADVDR--VS ARLEN----G VLTVTVPKVA GH-------- 
Hsp21.8-ER   ERAA-----G RFWRRFRMPP GADVGR--VA ARLDD----G VLTVTVPKVP GH-------- 
Vv21.3       ERAT-----G KFWRQFRLPA NADLDR--IK AHLEN----G VLRITIPKLA ED-------- 
At22         ERSY-----G KFWRQFKLPD NVDMES--VK AKLEN----G VLTINLTKLS PE-------- 
Hsp23.2-ER   ERSY-----G RFWRQLRLPD NADLDS--IA ASLDN----G VLTVRFRKLA PD-------- 
Hv17.1       RRGG------ -FARKFRLPG MVDADG--IT AEYAH----G VLTVTVPRMH NR-------- 
Hsp18.8-CV   GRRS------ -FARKFRLPA MVDADG--IS AEYTH----G VLRVTVPRLH TR-------- 
Zm17.2       DARS------ -FDRKFRLPG MVDVDG--IS AAYTH----G VLTVKVPRMH TR-------- 
Vv15.7       PAKS------ -FMRKFRLPD MIDIDG--IS AGYED----G VLTVTVPRSF VR-------- 
At15.4       PLKT------ -FKRKFRLPE SIDMIG--IS AGYED----G VLTVIVPKRI MT-------- 
Lp16.1CIII   ERNP----PL KLMRKFKLPD YCNVSA--IT AKCEN----G VLTVVVEKMP P--------- 
At17.4CIII   ERRL----AQ NLVKKFRLPE DADMAS--VT AKYQE----G VLTVVIKKLP PQ-------- 
Hsp18.6-CIII ERRAS---PR AFARKFRLPE DADTGG--IS ARCEN----G VLTVTVKKRP PP-------- 
At17.6-CII   ERRM-----G KFMRKFQLPE NADLDK--IS AVCHD----G VLKVTVQKLP PP-------- 
At17.7       ERRM-----G KFMRKFQLPD NADLEK--IS AACND----G VLKVTIPKLP PP-------- 
Hsp18.0-CII  ERRM-----G KFMRKFVLPD NADVDK--IS AVCQD----G VLTVTVEKLP PP-------- 
Hsp19.0-CII  ERRM-----G KFMRRFPLPE SADLDG--VR AEYKD----G VLTVTVDKKP PP-------- 
At15.7       EREAFSGGGS EFLRRIELPE NVKVDQ--VK AYVEN----G VLTVVVPKDT SS-------- 
Gm16.2       ERGTGKGG-- -FSREIELPE NVKVDQ--IK AQVEN----G VLTIVVPKDA TP-------- 
Hsp16.0-Px   ERGR-----P EFAREVALPA EVRVEQ--IR ASVDN----G VLTVVVPKEP AP-------- 
Vv22.4       WEHG------ -YVRRLELPE NADWRR--IE ASVK---DEI YLEIRIPKCD IP-------- 
At21.7       WEHG------ -YVRRLELPS DADAKY--SE AFLSNNDDYS FLEIRIPKIN SK-------- 
Hsp22.3-CVI  WEHG------ -FVRRVELPE DADWRK--VE AFFD--DGEG LLEIKVPKSG DA-------- 
Zm21.9       WEHG------ -FVRRVELPE DAEWRK--VE AHFD--DCEG ALEIKVPKTA DD-------- 
Clustal Co                                    *           * : . :              

             ....|....| ....|....| ....|....| ....|....| ..
                305        315        325        335             
Le26.2       ---KKVIDVQ IN-------- ---------- ---------- --
Vv25.0       ---RKVIDVQ IQ-------- ---------- ---------- --
At25.3       ---RKVIDVQ IQ-------- ---------- ---------- --
Hsp26.7-P    ---RKVIDVQ VQ-------- ---------- ---------- --
Ta26.7       ---RKVIDVK VQ-------- ---------- ---------- --
Zm26.4       ---RKVIDVQ VQ-------- ---------- ---------- --
At23.6-M     ---ERNDVRQ IEIN------ ---------- ---------- --
At23.5-M     ---ERNNIRH INVD------ ---------- ---------- --
Hsp26.2-MI   ---ERKDVFQ VNVE------ ---------- ---------- --
Hsp24.0-MI   ---QRRDVFQ VNVE------ ---------- ---------- --
Zm22.8       ---RKRNVTE VKVQ------ ---------- ---------- --
Hsp23.6-MII  ---RKRSVTE VKVR------ ---------- ---------- --
At26.5       ---PKKNVQE ISVE------ ---------- ---------- --
Vv26.3       ---KGKDVKE VQIQ------ ---------- ---------- --
So17.4       GEKPN-EGPA PLVS---GPA CC-------- ---------- --
Sb17.4       AEKPN-EVPA AVEAADGGPA C--------- ---------- --
Zm17.4       PEKPAADAPA LAPVPVEAGP C--------- ---------- --
Cd16.6       AEMPE--AKA AIEA---GP- ---------- ---------- --
Hsp17.9B-CIX APVPAVVAPA VEAKAIEASP ---------- ---------- --
Zm16.6       --KPQ--VRA VEISG----- ---------- ---------- --
So16.5       --KPQ--VRA VEISG----- ---------- ---------- --
Hsp16.6-CVIII--KPQ--LKA IPISG----- ---------- ---------- --
Hsp16.9B-CI  --KPE--VKA IEISG----- ---------- ---------- --
Hsp16.9A-CI  --KPE--VKA IEISG----- ---------- ---------- --
Hsp16.9C-CI  --KPE--VKA IEISG----- ---------- ---------- --
Zm17.2       --KPE--VKA IEISG----- ---------- ---------- --
Ta16.9B      --KPE--VKA IQISG----- ---------- ---------- --
Hsp17.9A-CI  --KPD--VKS IQISG----- ---------- ---------- --
Hsp18.0-CI   --KPD--VKS IQVTG----- ---------- ---------- --
Hsp17.4-CI   --KPD--VKS IQITG----- ---------- ---------- --
Hsp17.7-CI   --KPD--VKS IQISG----- ---------- ---------- --
Zm17.9       --KAD--VKN VQITG----- ---------- ---------- --
At17.4CI     --KPE--VKS VDISG----- ---------- ---------- --
Ta20.1       --KAY--GRI IPITN----- ---------- ---------- --
Hv19.3       --KAY--GRI IPITN----- ---------- ---------- --
Ps19.8       --KAY--GRN IPITN----- ---------- ---------- --
Hsp18.8-CX   --KPH--ARI IPITN----- ---------- ---------- --
Vv16.4       --MKHSQKNM VEISGDD-EA HAPKGLGRFV CCKA------ --
Pt17.6       --TKPKNK-A VEISGDDGEK HVSRGLGRFV CCKA------ --
Hsp17.8-CXI  --KRHAGN-- GKAAGDD--- -------KPV CCRFWP---- --
Zm22.7       ---RGREPRV ISIAGGD--M GGVDVA---- ---EVMASKA EM
Hsp21.8-ER   ---RGREPRV VAIDGAG--A GDMEAE---- ---VVKASKA EM
Vv21.3       ---RKKQAKV VNIAEET--N SGED------ ----VMATKS EM
At22         ---KVKGPRV VNIAAEE-DQ TAKISS---- ----SESKEL --
Hsp23.2-ER   ---QIKGPRV VGIASAGGDD GGKKSIGGAG EGQNQQAKKV EL
Hv17.1       ---ARPMVNL IGSG-----P ACDPVARAA- ---------- --
Hsp18.8-CV   ---ARPVVNL AGGGGGGGGP ACDPVARAA- ---------- --
Zm17.2       ---ARPVVDI LGAG---AGP ASDHAARAA- ---------- --
Vv15.7       ---RGFYIDP GDLP-----E QLELLARAA- ---------- --
At15.4       ---R-RLIDP SDVP-----E SLQLLARAA- ---------- --
Lp16.1CIII   ----PSKAKT VKVA------ ----VS---- ---------- --
At17.4CIII   ----PPKPKT VQIA------ ----VS---- ---------- --
Hsp18.6-CIII ----EKKTKS VQVT------ ----IA---- ---------- --
At17.6-CII   ---EPKKPKT IQVQ------ ----VA---- ---------- --
At17.7       ---EPKKPKT IQVQ------ ----VA---- ---------- --
Hsp18.0-CII  ---EPKKPKT IEVK------ ----VA---- ---------- --
Hsp19.0-CII  ---EPKKPRV VEVK------ ----VAGAGE PKGKGK---- --
At15.7       ---KSSKVRN VNITSKL--- ---------- ---------- --
Gm16.2       ---KTPKVRN INITSRL--- ---------- ---------- --
Hsp16.0-Px   ---ARPRTRP IAVSSKL--- ---------- ---------- --
Vv22.4       ---HGKEEGA EDSE------ ---------- ---------- --
At21.7       ---NKF---- ---------- ---------- ---------- --
Hsp22.3-CVI  ---HQAAAAT A--------- ---------- ---------- --
Zm21.9       ---DDAHHAT A--------- ---------- ---------- --
Clustal Co