>AlternativePrimerA, with 'V' replced by 'A': AAGCAGTGGTATCAACGCAGAGTCGCAGTCGGTACTTTTTTCTTTTTTV
AAGCAGTGGTATCAACGCAGAGTCGCAGTCGGTACTTTTTTCTTTTTTA
>AlternativePrimerC - with 'V' replaced by 'C'
AAGCAGTGGTATCAACGCAGAGTCGCAGTCGGTACTTTTTTCTTTTTTC
>AlternativePrimerG with 'V' replaced by 'G'
AAGCAGTGGTATCAACGCAGAGTCGCAGTCGGTACTTTTTTCTTTTTTG

>MINTadapter5prime | At the 5'end is added: 5'-AAGCAGTGGTATCAACGCAGAGTACGGGGG-3' : NOTE: Only upper- and lower-case A, C, G, T, and N characters should be used in Newbler trimming. Do not use X or N as masking characters.
AAGCAGTGGTATCAACGCAGAGTACGGGGG

Sequences_for_trimming_abundant_sequences

>gi|48476450|gb|AY550963.1| Sparus aurata myosin heavy chain-like mRNA sequence
AAAGAATTCGGCAGAGGNNGAAAATGTCCGTCAAATCAATGACACGAGTGCACAGAAAGCACGTCTTCTG
ACAGAAAATGGTGAGTTCGGCCGTCAAATTGAGGAGAAGGAAGCTCTGGTCTCCCAGCTGACCAGAGGCA
AACAGGCCTTCACACAGCAGATTGAGGAGCTGAAGAGACAGACTGAAGAGGAGGTTAAGGCCAAGAATGC
TCTTGCCCATGGACTGCAATCAGCCCGCCATGACTGTGACCTGCTGAGGGAGCAGTTTGAGGAGGAGCAG
GAGGCCAAGGCTGAGCTGCAGCGTGGAATGTCCAAGGCCAACAGTGAGGTAGCATCAGTGGAGAACAAAG
TATGAAACTGATGCTATCCAGCGCACTGAGGAGCTTGAGGAAGCCAAGAAGAAGCTGGCCCAGCGTCTCC
AGGAGGCTGAGGAGCAGATTGAGGCAGTGAATTCCAAGTGTGCTTCTCTTGAGAAAACCAAACAGAGGCT
TCAGAGTGAGGTGGAGGACCTCATGATTGATGTAGAGAGGGCCAATGGACTGGCTGCTAACCTGGACAAG
AAGCAGAGGAACTTTGACAAGGTGTTGGCAGAGTGGAAACAGAAGTATGAGGAGGGTCAGGCAGAGCTTG
AGGGATCTCAGAAAGAGGCTCGCTCTCTTGGCACTGAGCTGTTCAAGATGAAGAACTCTTATGAAGAAGC
TCTGGATCAGCTGGAGACCATGAAGCGTGAAAACAAGAACCTGCAGCAGGAGATCTCAGATCTAACTAAA
CAGATCGGTGAGACTAATAAGAGCATNCATGAGCTGGNGANGGCCAAGNNGCAGGTGGGGACAGAGANGT
CTGAGATCCAGACAGCTCTTGAAGAGGCTGAGGAAACTCTGGAACATGAAGAGTCCAAGATCCTGCGTGT
CCAGCTGGAGCTCAACCAGATTAAAGGTGAGGTGGACAGGAAGCTGGCAGAGAAAGATGAAGAGATGGAG
CAGATCAAGAGGAACAGCCAGAGGGTGACTGACTCCATGCAGGGCACCCTGGATTCTGAAGTCAGGAGCA
GGAATGATGCCCTGAGAATCAAGAAGAAGATGGAGGGAGACCTGAATGAGATGGAGATTCAGTTGAGCCA
CGCCAATCGCCAGGCTTCTGAGTCCCAGAAGCAGCTGAGGCTTGTGCAGGCACAGCTGAAGGATGCCCAA
CTGCACCTTGATGATGCTGTCAGAGCTCAGGAAGACGTCAAGGAACAAGCTGCTATGGTGGAGCGCAGAA
ACGGTCTTATGGTAGCTGAAATTGAGGAACTTAGAGCTGCTCTGGAACAGACGGAGAGAAGCCGCAAAAT
TGCAGAGCAGGAGTTGGTGGATGCCAGTGAGCGTGTTGGACTTCTGCACTCTCAGAACACAAGCCTTATG
AACACTAAGAAGAAGCTTGAGACTGATCTGGTCCAGATCCAGAGTGAAGTGGATGACACTGTTCAGGAAG
CAAGGAATGCAGAGGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGAAGAA
GGAGCAGGACACTAGCGCTCACCTGGAGAGGATGAAGAAGAACCTTGAGGTTGCTGTTAAGGATCTGGTG
CATCGCCTGGATGAGGCAGAGAACCTGGCAATGAAGGGTGGCAAGAAGCAGCTCCAGAAACTTGAGTCCA
GGGTGCGTGAGCTGGAGACAGAGGTTGAGGCTGAGCAGAGACGTGGAGCAGATGCTGTTAAGGGTGTCCG
CAAATATGAGAGGAGGGTGAAGGAGCTCACCTATCAGACTGAAGAGGACAAGAAAAATGTTACCAGGCTG
CAGGATCTGGTTGACAAGCTGCAACTGAAGGTGAAGGCCTACAAGAGGCAGGCTGAGGAAACGGAGGAGC
AGGCCAACGTTCATCTGTCCAAGTGCAGAAAGGTCCAGAACGAGCTGGAGGAGGCTGAGGAGCGCGCTGA
CATTGCAGAGTCCCAGGTCAACAAAATGAGAGTAAAGTCCCGTGATTCCGGCAAGGGAAAAGAGGCAGCA
GAGTAACGCGCTGGCTGATCAGGAGTTTTATAATCATATAATATGATGTGAAATACACTGTATAAATAAA
TGTTTACTTCTCACTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

>gi|33340044|gb|AF304559.1| Sparus aurata troponin mRNA, partial cds
TTCGTGGAGAGGGACCAGGCCGGCAAGAGCGAGGAAGAGTTGGCAGAGTGCTTCCGCGTGTTCGACAAGA
ACGGCGACGGCTACATCGACAGAGAGGAGTTCGCCATCATCATCCTTATAAACATCCTCAACAGACAGCT
AGGGCACCTGGCAGGGGTCGCTGGCTGGGGCTACACTCTTCCCCAGCCTCGCTTGTGATTTGCATTTTAT
TAACAGGCACGTGTCCATCAGCGACCCCCTCCCCCCATGCTGGTGAAGCTAATCTGATCTAGAATCTAGT
CTAGAGAGAACAGCAGCCTGTTGTCTGCTGCTTAGTCCAATTAAGTCAACCAATTCACTGTACAAAGTAA
TTTGCAAGAACAGAATAAATGAACTAACTCATGCTCACTTGTAGTTGGTCTGTCGTATTTATGGTCTGTG
TAAAAAAATTTTGTTTTGAGGAATATCAATAAAATCTGTTTGTTTCTTC

>gi|6653227|gb|AF190473.1| Sparus aurata skeletal alpha-actin mRNA, complete cds
CAACAGCACTTGTTGGGTCTTTCTCTCTCCGAGCCGCAGACACACTCCTAAGAAGCCATCATGTGTGACG
ACGATGAGACTACCGCCCTTGTGTGCGACAACGGCTCAGGCCTGGTGAAGGCTGGCTTCGCCGGAGACGA
TGCCCCCAGGGCAGTGTTCCCCTCCATCGTCGGCCGCCCCCGTCATCAGGGTGTCATGGTCGGTATGGGT
CAGAAGGACTCCTACGTCGGCGACGAGGCCCAGAGCAAGAGGGGTATCCTGACTCTGAAGTACCCCATCG
AGCACGGCATCATCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACAACGAGCTGAG
AGTTGCCCCCGAGGAGCACCCCACCCTGCTCACTGAGGCTCCCCTCAACCCCAAGGCCAACAGAGAGAAG
ATGACCCAGATCATGTTTGAGACCTTCAACGTCCCCGCCATGTATGTTGCCATCCAGGCTGTGCTGTCCC
TGTACGCTTCCGGTCGTACCACTGGTATTGTGCTGGATGCTGGTGATGGTGTGACCCACAACGTCCCAGT
CTATGAGGGTTACGCCCTGCCCCACGCCATCATGCGTCTGGACCTGGCTGGTCGCGATCTTACCGACTAC
CTGATGAAGATCCTGACTGAGCGTGGCTACTCCTTCGTCACCACCGCCGAGCGTGAGAATCGTGCGCGAC
ATCAAGGAGAAGCTTGCTATGTGGCTCTGGACTTCGAGAACGAGATGGCCACCGCTGCCTCTTCTTCCTC
TCTGGAGAAGAGCTACGAGCTTCCCGACGGTCAGGTCATCACCATCGGTAACGAGAGGTTCCGTTGCCCC
GAGACCCTCTTCCAGCCTTCCTTCATTGGTATGGAGTCTGCTGGTATCCATGAGACCGCCTACAACAGCA
TCATGAAGTGCGACATTGACATCCGTAAGGATCTGTACGCCAACAATGTGCTCTCCGGTGGTACCACCAT
GTACCCTGGTATTGCTGACCGTATGCAGAAGGAGATCACTGCTCTGGCCCCCAGCACCATGAAGATCAAG
ATCATTGCCCCTCCCGAGAGGAAGTACTCCGTCTGGATCGGTGGCTCTATCCTGGCTTCCCTGTCCACCT
TCCAGCAGATGTGGATCTCCAAGCAGGAGTACGACGAGGCAGGCCCCAGCATTGTCCACAGGAAGTGCTT
CTAAATCTTCCATCTTCTTCTCCAGCTTCTCCATCATCTCCACAAACACCACCAGCTATCCGGGGATCAT
GCGAGAAGGAGGATCAACCTCTGCTGCACAGCAACACTCTGCTGTCAATGGACTTTCTGTCTGCGCTGCT
GACATTCATATGCATTTTGTTATCCTTTTAAAAATGTGCATATTTTAGAGCTGTCTGTTGTCTGTGTTGC
ACGGAGAGAGGCTGTGAAAACATCACTGAACCATGCATCCACCCAAAAAAAAACTTTAATAAAAAAATAA
CAACAAAACATGTTGCCCAACACATGTGAATGTAAGTGTATATACATATTGAT

>gi|34014737|gb|AY362763.1| Sparus aurata beta-actin mRNA, partial cds
GACCAACTGGGATGACATGGAGAAGATCTGGCATCACACCTTCTACAACGAGCTGAGAGTTGCCCCTGAG
GAGCACCCAGTCCTGCTCACAGAGGCCCCCCTGAACCCCAAAGCCAACAGGGAGAAGATGACCCAGATCA
TGTTCGGGACCTTCAACACCCCCGCCATGTACGTTGCCATCCAGGCTGTGCTGTCCCTGTATGC

>gi|5852837|gb|AF150904.1| Sparus aurata myosin light chain 2 mRNA, complete cds
TGGCTTTGGCTTAGGCTTCTCTTCTTGACCACCAACAACCCCAGAAACTTGAGGATGGCACCCAAGAAGG
CCAAGAGGAGGCAGCAGCAGGGCGAGGGTGGATCCTCCAATGTGTTCTCCATGTTTGAGCAGAGCCAGAT
CCAGGAGTACAAGGAGGCTTTCACAATCATTGACCAGAACAGAGATGGCATCATCAGCAAGGACGATCTT
AGGGACGTGCTGGCCACCATGGGCCAACTGAATGTGAAGAATGAGGAGCTGGAGGCCATGGTGAAGGAGG
CCAGCGGCCCCATCAACTTCACCGTCTTTCTGACCATGTTCGGCGAGAAGCTGAAGGGTGCTGATCCCGA
GGACGTCATCGTGAGCGCTTTCAAGGTCCTGGACCCCGAGGCCACTGGCGCCATCAAGAAGGAATTCCTT
GAGGAGCTCCTGACCACCCAGTGCGACAGGTTCACCGCTGAGGAGATGACCAACCTGTGGGCTGCTTTCC
CCCCTGATGTGGCTGGCAATGTGGACTACAAGAACATCTGCTACGTCATCACACACGGAGAAGAGAAGGA
GGAATAAATCCCCCTCTCTTTCAAGATCCTTACCTCCGCTCAAATCCCATATACTCGACGCAACATCTAC
TCTACTCACTCTTCTCCGATGCCGTGGCTCCCTCGGACACTCTCGCGCCCTCGGCCCGCTCTGTCGCTTT
GCAGCTCACTACAAAAAGAACTTGTCTCCTGTTCTTGAGATACTCAGTGAGAGGACTGGGGGCTGTGGGG
TTGTTTGTGTGTGATTACCAACAGGTGAACATGGGATTATTTTCAATAAAAATAATCCTTGTGGCACTGA
AACTCTCTCTCCATCTCTGTCCCTGCCTCTTGTTCCCCCTGCTTTTCCTCCCATCACTCATTCTGTCCTT
CTGCGTTGACGCCAACAGTGCATGCATCATGCCTATGTACAGCGCGTATGCATATGCAGTCCAGTGTATA
CAGTGGCCAGTCAGACATATCTCTTGGGTGCTGTGGTGCAAGCACAGCCGCTCACTTCAAACAAGTAAGC
GGCCTGACCCGAGTGGTCTGTTAGTCTCAACCTGACACAGAGTGTTTTATGGACTCGTCCCTTTGTTTGT
ATCAGGGAGGATAGCACAGTGAAGAGTGGGAGTACCGTACTATAATAGATTGCCTACTCCTTCTCTTTAA
TCTGTCTCTCCTTCTCTTAAACACAGGCATGACAGGAAAAGTTGCAGTGAAAATGGGAAAGCATGATTTG
GTTCAAATCTTGTAATTGGAGAAAGAGATGGTGAAAGATGGTGAGTGGGAGGGAGAGATGAAATAAACGA
AAGTGAAATGTCTTGTTTTGGTCTCTTTTTTCTCCGACTCACTGCTGTTTCTCTCCTGTTTTCATGACTG
TACCAAATAAAGAAGTACAAATAAAATCCACTATCTTTCGTAAAAAAAAAAA

>gi|5852835|gb|AF149756.1| Sparus aurata fast skeletal myosin light chain 3 mRNA, complete cds
CAACTCTCCAACATGACCGAACAGGCCGAGTTCAGTGCCGACCAGATTGAGGACTTCAAGGAGGCTTTCG
GTCTCTTTGACAGAGTCGGTGACAGCCAGGTGGCCTTCAACCAGGTTGCTGACATCATGCGCGCTCTGGG
CCAGAACCCCACCAACAAGGACGTTACAAAGATTCTGGGCAACCCCTCCGCCGACGACATGGCCAACAAG
AGGCTCAACTTCGAGGCTTTCCTGCCCATGCTGAAGGAGGTCGACGCCTTGCAGAAGGGCACCTACGACG
ACTACGTTGAGGGCCTGCGCGTCTTCGACAAGGAGGGCAACGGCACAGTCATGGGCGCTGAGCTGCGCAT
CGTGCTGTCCACCCTGGGAGAGAAGATGACCGAGCCTGAGATTGATGCCCTCATGGCCGGCCAGGAGGAC
GAGAACGGCAGTTTGCACTATGAGGCTTTCGTCAAGCACATCATGTCTGTGTAAGAGGCCGGCAGCAGGA
GTGCTGAAGAACAGCCCGACGGTGTTCAGGACATCTACACTGTTGTCAAAGACCAACCAAGGAAAGACAA
GGACTATGTACAAGGGATGTTGAAGCAAACCCATCTTGTTGTTTTATTTTGTTTTTCCCTTTCCATTTCG
TCTCACMCSCCCTCCTCCCTCTCTCGGGACGCCACCATCACCTCTACACCGTAGACCCACCACCACTCGG
CCTCCACTCCCCCCTACCCTCCGGGGCACGTCTACACATGCGCATGGGTGAGGAACGGGGAAAAGGGGTG
AGCGCTCATCAAGTGAGGGTAGATCCTCCCTTCCCACCGCCACTCATTCAGTCTCGCCATCACTGGTCCA
GCAATGCAAAGGTGCTGAGAATGGTCGTGGATAGACGCCAAAAAAGTCTCCCCGCTTCCCTGAAAAGTTT
TATTTATTGTCACTCTGTTCATGTCAGAATAAACTTTTCCAACGTAAAAAAAAAAAAAAAAA

>gi|32895273|gb|AY326430.1| Sparus aurata alpha-tubulin mRNA, partial cds
CTGGACAGGATCCGCAAACTGGCTGACCAGTGCACAGGCCTCCAAGGTTTYCTCATCTTCCACTCCTTTG
GTGGAGGAACCGGCTCTGGCTTCACCTCTCTGCTGATGGAGCGCCTTTCTGTCGACTACGGCAAAAAGTC
CAAGCTGGAGTTTGCCGTGTACCCAGCTCCCCAGGTGTCCACGGCCGTGGTGGAGCCATACAACTCCATC
CTGACCACCCACACCACCCTGGAGCACTCCGACTGCGCCTTCATGGTGGACAATGAGGCCATCTATGACA
TCTGCCGCAGAAACCTGGACATCGAGCGTCCGACCTACACCAACCTCAACAGGCTGATTGGACAGATCGT
CTCCTCCATCACCGCCTCCCTGCGCTTCGACGGCGCCTTGAACGTGGACCTGACGGAGTTCCAGACCAAC
TTGGTGCCCTACCCTCGTATCCACTTCCCTCTGGCCACCTACGCCCCCGTTATCTCAGCAGAGAAGGCCT
ATCATGAGCAGCTGTCAGTTGCTGACATCACCAATGCCTGCTTCGAGCCCGCCAACCAGATGGTCAAATG
CGACCCTCGCCATGGGAAGTACATGGCCTGCTGCCTCCTGTACCGTGGTGACGTTGTACCCAAAGATGTG
AACTCCGCCATCGCCACCATCAAAACCAAGCGTACCATCCAGTTTGTGGACTGGTGTCCCACAGGCTTCA
AGGTGGGCATCAACTACCAGCCTCCCACAGTGGTTCCTGGAGGAGATCTGGCCAAGGTGCAGAGGGCCGT
GTGCATGCTGAGCAACACCACCGCCATYGCCGAGGCCTGGGCTCGTCTCGACCACAAGTTCGACCTGATG
TACGCCAAGAGGGCCTTCGTCCACTGGTACGTCGGTGAGGGTATGGAAGAGGGTGA

>gi|15281826|gb|AF398343.1|AF398343 Danio rerio 28S ribosomal RNA gene, partial sequence 1500-2400
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCTTGGAGCCGG
GCGTGGAATGCGAGACGCCCAGTGGGCCTTTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACG
CCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCAGG
ACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTG
AAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGACGGCAGAAGGAGCCCCCCCCTCCGGG
GGGGGAAAGGGTAAGCCTCGACGAGTAGGAGGGCCGCCGCGGTGGCGCGGAAGCCTAGGGCGTGGGCCCG
GGTGGAGCCGCCGCGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAGCTTTGAAGGCCGAA
GTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTAAGGGATGGGCGAACGCCG
TTCGGAAGGGAGGAGCGATGGCCTCCGTCGCCCCCGGCCGATCGAAAGGGAGTCGGGTTCAAATCCCCGA
ACCCGGAGTGGCGGAGACGGGCGCCGCGAGGCGCCCAGTGCGGTGACGCAAACCAACCCGGAGAAGCCGG
CGGGAGCCCCGGGAAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA
GAGGGTGTAAATCTCGCGA

>gi|148614872|gb|EF417169.1| Danio rerio large subunit ribosomal RNA gene, partial sequence
GCCCAGCGCCGAATCCCCGCCCCGTGCCAGGGGCGAGGGAAATGTGGCGTACGGAAGATCGCTCTCTCTC
GGCGCGGGCCGGGGGTCTAAGTCCTTCTGATGGAGGCTTAGCCCGTGGACGGTGTGAGGCCGGTAACGGC
CCCCGCCCCGCCGGGGTACGGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGGTGGTAA
ACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCGACAAGTACCGTGAGGGAAAGTTGAAAAGA
ACTTTGAAGAGAGAGTTCAACAGGGCGTGAAACCGTTGAGAGGTAAACGGGTGGGGTCCGCACGGTCCGC
CCGGAGGATTCAACCCGGCGGGGCTGGTCGGCCCGTCCGGTGCGCTCGGATCCCCCCCCTCTCGGGGGAG
GGGGACCGGCGCCCGGACGGAGCTCGGCCGCCGCCGGGCGCACTTCCTCCGTCGGCGGTGCGCCGCGACC
GGCTCCGGTTCGGCTTGGAAGGGTTCGGGGGCGAAGGTGGCTCGCGGCTCCGGCCTCGAGCTTTACAGCG
CCCCCYCGCCCCGACTTCGCCGCTTGCTACCCGGGGCCGCGGGCAGTGTCCTCCGCGCCTTCTCTCCGGC
CCCTCTCTCGGGGGGGGCTCGGAGGGACGGGGCCCCTCGCTCCCGGCGCGTGCGTCGACCGGAACGGACT
GTCCTCAGTCCGTTTCCGACCGCGCCGTGCCGCCCAGGGCGGGGACAGGCCCACGTCCAAAAGGGCGCCC
GAGGTCCGCGGTGATGCCGGCCACCCACCCGACCCGTCTTGAAACACGGACCAAGGAGTCTAACGCGCGC
GCGAGTCAGAGGGTGCCCTCGAGCCCCCACGGCGCAATGAAGGTGAAGGCCGGCGCGCGCCGGCCCAGGT
GGGATCCCCCCGCCCCGGCGGGGGGCGCACCACCGGCCCGTCTCGTCCGCTCTGTCGGGGAGGTGGAGCT
AGAGCGCGTGCGATGGTACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTG
GTGGAGGCCCGCAGCGGTCCTGACGTGCAAATCGGT