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[]
["Additional file 1: Identification of differentially expressed protein spots among L. plantarum LC 56, LC 804 and 299V in standard growth conditions."]
[]
["Evolutiona)","Inter-strain occurrenceb)","Protein identification","NCBI accession number for L. plantarum WCFS1","Gene symbol","Spot number","Sequence coverage (%)","Peptides matched","Normalized volume in standard conditions (MRSC)c)",null,null,"Functional category"]
[{"$nulls":8},"LC 56","LC 804","299V"]
["_ää",3,"α-small heat shock protein","gi|28377077","hsp1",1,59,"23/26","0.028±0.015","0.088±0.013","0.282±0.057","Posttranslational modification, protein turnover, chaperones"]
[null,null,"chaperonin GroEL","gi|28377591","groEL",76,69,"150/178","7.367±0.298","9.379±0.638","12.992±1.464"]
[null,null,"3-oxoacyl-[acyl-carrier protein] synthase II","gi|28378365","fabF",88,43,"23/28","0.064±0.007","0.257±0.009","0.301±0.011","Lipid transport and metabolism"]
[null,2,"transcription regulator (putative)","gi|28377930",null,32,52,"19/21","-","0.088±0.009","0.213±0.026","Transcription"]
[null,null,"F0F1 ATP synthase subunit delta","gi|28378941","atpH",90,41,"9/9","-","0.057±0.005","0.093±0.016","Energy production and conversion"]
[null,null,"glucose-6-phosphate 1-dehydrogenase","gi|28379186","gpd",26,59,"52/56","-","0.119±0.014","0.166±0.008","Carbohydrate transport and metabolism"]
[null,null,"adenylate kinase","gi|28377853","adk",28,42,"13/16","-","0.023±0.004","0.081±0.016","Nucleotide transport and metabolism"]
[null,null,"β-phosphoglucomutase","gi|28376999","pgmB1",35,77,"63/68","-","0.036±0.002","1.128±0.125","General function prediction only"]
[null,null,"alkaline shock protein (putative)","gi|28377758","asp2",2,64,"34/39","-","0.381±0.011","1.660±0.013","Function unknown"]
[null,null,"alkaline shock protein (putative)","gi|28377757","asp1",3,83,"57/63","-","0.213±0.057","0.925±0.046"]
[null,null,"protein of unknown function lp_2652","gi|28379164",null,31,72,"14/17","-","0.055±0.005","0.113±0.006"]
[]
["‾ææ",3,"glycine hydroxymethyltransferase","gi|28378947","glyA",65,33,"35/42","0.296±0.014","0.111±0.010","0.069±0.008","Amino acid transport and metabolism"]
[null,null,"bifunctional GMP synthase/glutamine amidotransferase protein","gi|28377744","guaA",80,48,"55/57","0.295±0.027","0.191±0.009","0.017±0.002","Nucleotide transport and metabolism"]
[null,2,"co-chaperonin GroES","gi|28377590","groES",38,59,"18/21","0.758±0.084","0.089±0.011","-","Posttranslational modification, protein turnover, chaperones"]
[null,null,"aspartate-semialdehyde dehydrogenase","gi|28379104","asd2",63,50,"22/26","0.222±0.008","0.174±0.022","-","Amino acid transport and metabolism"]
[null,null,"adenylate kinase","gi|28377853","adk",61,26,"13/13","0.104±0.042","0.025±0.006","-","Nucleotide transport and metabolism"]
[]
["_ä‾",2,"tyrosyl-tRNA synthetase","gi|28379288","tyrS",33,27,"15/18","-","0.069±0.010","0.053±0.007","Translation, ribosomal structure and biogenesis"]
[null,null,"ATP-dependent Clp protease proteolytic subunit","gi|28377639","clpP",77,67,"24/26","-","0.177±0.048","0.165±0.030","Posttranslational modification, protein turnover, chaperones"]
[null,null,"phosphotransacetylase","gi|28377658","eutD",10,39,"17/18","-","0.277±0.030","0.271±0.044","Energy production and conversion"]
[null,null,"glutathione reductase","gi|28270115","gshR1",24,47,"21/25","-","0.286±0.003","0.239±0.042"]
[null,null,"phosphoglyceromutase","gi|28379560","gpmA",9,73,"60/70","-","1.931±0.201","1.684±0.148","Carbohydrate transport and metabolism"]
[null,null,"glycine/betaine/carnitine/\ncholine ABC transporter, ATP-binding protein","gi|28378307","opuA",18,34,"15/21","-","0.056±0.001","0.054±0.004","Amino acid transport and metabolism"]
[null,null,"orotate phosphoribosyltransferase","gi|28379197","pyrE",30,42,"16/17","-","0.083±0.012","0.077±0.008","Nucleotide transport and metabolism"]
[null,null,"dihydrodipicolinate synthase","gi|28378743","dapA1",15,44,"17/21","-","0.068±0.005","0.058±0.008","Cell wall/membrane/envelope biogenesis"]
[null,null,"protein of unknown function lp_1972","gi|28378614",null,89,48,"28/37","-","0.073±0.011","0.062±0.008","Function unknown"]
[]
["_ _ä",1,"glyceraldehyde 3-phosphate dehydrogenase","gi|28377642","gapB",29,49,"25/33","-","-","0.112±0.019","Carbohydrate transport and metabolism"]
[null,null,"citrate lyase, beta chain","gi|28377896","citE",17,56,"27/32","-","-","0.092±0.014","Carbohydrate transport and metabolism"]
[null,null,"cystathionine beta-lyase","gi|28377181","metC1",37,33,"16/20","-","-","0.087±0.006","Amino acid transport and metabolism"]
[null,null,"bile salt hydrolase","gi|28379847","bsh1",11,54,"20/23","-","-","0.156±0.054","Cell wall/membrane/envelope biogenesis"]
[]
["‾æ_",3,"elongation factor G","gi|28377830","fusA2",83,63,"77/91","0.286±0.031","0.076±0.006","0.075±0.009","Translation, ribosomal structure and biogenesis"]
[null,null,"galactokinase","gi|28379798","galK",79,49,"49/52","0.684±0.058","0.026±0.008","0.024±0.005","Carbohydrate transport and metabolism"]
[null,null,"oxidoreductase (putative)","gi|28377171",null,27,55,"24/26","0.343±0.028","0.065±0.009","0.076±0.007","General function prediction only"]
[null,null,"protein of unknown function lp_2253","gi|28378848",null,85,47," 30/36","0.256±0.015","0.109±0.025","0.094±0.011","Function unknown"]
[null,1,"ribosomal protein L12/L7","gi|28377498","rplL",54,56,"11/15","0.316±0.035","-","-","Translation, ribosomal structure and biogenesis"]
[null,null,"elongation factor P","gi|28378297","efp",44,56,"22/29","0.397±0.038","-","-"]
[null,null,"ribosomal protein L10","gi|28377497","rplJ",40,48,"33/36","0.664±0.056","-","-"]
[null,null,"ribosomal protein S30EA","gi|28377598",null,62,38,"10/10","0.154±0.017","-","-"]
[null,null,"transcription antitermination protein NusG","gi|28377493","nusG",39,30,"5/8","0.069±0.021","-","-","Transcription"]
[null,null,"UDP-glucose pyrophosphorylase","gi|28377616","galU",66,49,"22/24","0.266±0.044","-","-","Posttranslational modification, protein turnover, chaperones"]
[null,null,"phosphotransacetylase","gi|28377658","eutD",47,26,"11/12","0.064±0.008","-","-","Energy production and conversion"]
[null,null,"phosphoglyceromutase","gi|28379560","gpmA",43,73,"52/57","3.113±0.263","-","-","Carbohydrate transport and metabolism"]
[null,null,"glycine hydroxymethyltransferase","gi|28378947","glyA",50,23,"9/11","0.190±0.024","-","-","Amino acid transport and metabolism"]
[{"$nulls":5},86,18,"6/6","0.278±0.045","-","-"]
[null,null,"glycine hydroxymethyltransferase","gi|28378947","glyA",86,18,"6/6","0.278±0.045","-","-"]
[null,null,"2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase","gi|28378858","dapD",45,24,"15/16","0.205±0.022","-","-"]
[null,null,"phosphoribosylaminoimidazole-succinocarboxamide synthase","gi|28379221","purC",59,13,"9/11","0.197±0.013","-","-","Nucleotide transport and metabolism"]
[null,null,"glutaminase of carbamoyl-phosphate synthase","gi|28379201","pyrAA",53,13,"7/12","0.184±0.017","-","-"]
[null,null,"aspartate carbamoyltransferase","gi|28379203","pyrB",52,25,"14/17","0.213±0.017","-","-"]
[null,null,"enoyl-(acyl carrier protein) reductase","gi|28378371","fabI",51,22,"7/8","0.481±0.033","-","-","Lipid transport and metabolism"]
[null,null,"acetyl-CoA carboxylase, carboxyl transferase α-sub","gi|28378370","accA2",49,10,"4/6","0.070±0.003","-","-"]
[null,null,"stress-induced DNA binding protein","gi|28379536","dps",41,39,"13/14","0.388±0.059","-","-"]
[null,null,"dTDP-4-dehydrorhamnose 3,5-epimerase","gi|28377962","rfbC",42,35,"9/12","0.141±0.028","-","-","Cell wall/membrane/envelope biogenesis"]
[null,null,"NADH peroxidase","gi|28379083","npr2",48,26,"17/20","0.236±0.041","-","-","General function prediction only"]
[]
["‾ ‾æ",3,"protein of unknown function lp_2253","gi|28378848",null,78,50,"32/39","0.429±0.093","0.577±0.089","0.186±0.034","Function unknown"]
[null,2,"1-phosphofructokinase","gi|28378718","fruK",55,44,"19/21","0.163±0.006","0.153±0.011","-","Carbohydrate transport and metabolism"]
[]
["_äæ",3,"30S ribosomal protein S2","gi|28378686","fusA",68,63,"64/69","0.563±0.016","3.278±0.382","0.104±0.017","Translation, ribosomal structure and biogenesis"]
[null,null,"ribosomal protein L10","gi|28377497","rplJ",82,62,"43/45","0.065±0.007","0.725±0.032","0.413±0.015"]
[null,null,"elongation factor G","gi|28377830","fusA2",56,61,"89/110","0.867±0.071","1.275±0.149","0.038±0.010"]
[null,2,"aspartyl/glutamyl-tRNA amidotransferase subunit β","gi|28377927","gatB",25,58,"61/71","-","0.171±0.016","0.089±0.014"]
[null,null,"seryl-tRNA synthetase","gi|28377821","serS2",20,39,"24/28","-","0.140±0.021","0.056±0.007"]
[null,null,"methionyl-tRNA formyltransferase","gi|28378316","fmt",81,26,"12/16","-","0.117±0.006","0.046±0.003"]
[null,null,"elongation factor P","gi|28378297","efp",7,24,"12/14","-","0.487±0.014","0.159±0.022"]
[null,null,"lysyl-tRNA synthetase","gi|28377434","lysS",13,29,"18/26","-","0.085±0.008","0.038±0.019"]
[null,null,"response regulator","gi|28377004","rrp1",21,33,"19/21","-","0.082±0.005","0.032±0.004","Transcription"]
[null,null,"cold shock protein CspC","gi|28377809","cspC",67,79,"12/15","-","0.594±0.041","0.101±0.011"]
[null,null,"α-small heat shock protein","gi|28379699","hsp3",4,79,"28/38","-","0.707±0.043","0.342±0.082","Posttranslational modification, protein turnover, chaperones"]
[null,null,"co-chaperonin GroES","gi|28377590","groES",6,66,"54/62","-","0.649±0.029","0.444±0.071"]
[null,null,"L-lactate dehydrogenase","gi|28377422","ldhL1",58,11,"10/10","0.137±0.016","0.695±0.060","-","Energy production and conversion"]
[null,null,"6-phosphofructokinase","gi|28378549","pfkA",16,49,"31/39","-","0.462±0.068","0.121±0.013","Carbohydrate transport and metabolism"]
[null,null,"6-phosphogluconate dehydrogenase","gi|28378252","gnd2",87,47,"26/33","-","0.334±0.031","0.126±0.014"]
[null,null,"phosphoglyceromutase","gi|28379560","gpmA",5,39,"18/20","-","0.245±0.029","0.168±0.013"]
[null,null,"acetolactate synthase","gi|28377817","als",74,48,"32/44","-","0.062±0.007","0.038±0.005","Amino acid transport and metabolism"]
[null,null,"[acyl-carrier protein] S-malonyltransferase","gi|28378363","fabD",22,71,"22/24","-","0.133±0.011","0.038±0.004","Lipid transport and metabolism"]
[null,null,"cyclopropane-fatty-acyl-phospholipid synthase","gi|28379564","cfa2",64,21,"10/13","-","0.153±0.006","0.031±0.004","Cell wall/membrane/envelope biogenesis"]
[{"$nulls":5},72,29,"12/13","-","0.099±0.009","0.050±0.002"]
[null,null,"protein of unknown function lp_1872","gi|28378527",null,14,50,"11/17","-","0.106±0.007","0.079±0.009","Function unknown"]
[null,null,"protein of unknown fucntion lp_1583","gi|28378286",null,57,33,"17/19","0.045±0.014","0.089±0.011","-"]
[null,1,"oligopeptide ABC transporter, ATP-binding protein","gi|28378031","oppF",71,69,"38/43","-","0.124±0.017","-","Amino acid transport and metabolism"]
[null,null,"aspartate carbamoyltransferase","gi|28379203","pyrB",70,39,"17/19","-","0.280±0.041","-","Nucleotide transport and metabolism"]
[]
["‾æä",3,"glutathione reductase","gi|28379636","gshR4",19,53,"35/38","0.320±0.058","0.020±0.001","0.277±0.034","Energy production and conversion"]
[null,null,"stress-induced DNA binding protein","gi|28379536","dps",34,74,"40/52","0.106±0.020","0.037±0.011","1.200±0.210","Lipid transport and metabolism"]
[]
[]
["a) Spot intensity evolution from the sensitive strain (LC 56) to the resistant one (299V), based on their normalized relative volumes. The first symbol (-) represents the protein spot abundance in the 2D electrophoretic gels of the LC 56 strain;"]
[" the second and third symbols account for the spot intensity evolution from strain LC 56 to LC804 and from LC 804 to 299V, respectively. ä, increase; æ, decrease; - normalized relative volumes are not statistically different (p<0.05)."]
["b) Protein spot occurrence among L. plantarum LC 56, LC 804 and 299V strains (1 ≤ n ≤ 3)."]
["c) Normalized relative volumes, expressed as a percentage of total valid spots. Values are means ± standard deviations; n ≥ 3 for each strain. -, not detected."]