{"text_format":"3","workbook":"xls","n_sheets":1,"sheets":["plasmid.primers"],"read":"whole","date_system":"1904","formulas":"cached_only"}

{"sheet":"plasmid.primers","index":0,"n_rows":56,"n_cols":6,"rows_kept":56,"flags":["formulas_cached_only"]}
["Name","Sequence","Primer start site","Primer end site","Primer length","PCR product length"]
["Primer attL","GTAAGTGGTAAGAGTTTTTAGAATAATA",1,28,28]
["reverse primer 1","TGTCCACGTGTAATTTGTCGTT",1885,1906,22,1905]
["forward primer 2","ACGGCAGCCATCGATTTAGGA",1998,2018,21]
["reverse primer 2","ACTCCAGGACTTGCTTCTCGGCT",3808,3830,23,1832]
["forward primer 3","ACCTATGTTGCCACTAGTTGCTCT",3859,3882,24]
["reverse primer 3","AGTCAACTGGCCACCCTCATCGT",5739,5761,23,1902]
["forward primer 4","AGTGAAGCTCTGTGGTTGACG",5774,5794,21]
["reverse primer 4","AGCTGACTGCTTTGGGTTGTCAC",7718,7740,23,1966]
["forward primer 5","GGACGATGAGTCTCGAAAATCGGGC",7873,7897,25]
["reverse primer 5","TGCCTTTTGAGGTGTGCTGTGCT",9667,9689,23,1816]
["forward primer 6","TGCGAGGGTGAGGTTTTATTTTCGGC",9758,9783,26]
["reverse primer 6","TGCTGCATCGGATCAAAGCGA",11657,11677,21,1919]
["forward primer 7","TGTGGCACGTGGTCAAGCGA",11717,11736,20]
["reverse primer 7","AGGAAGTTGTATGGCACCAGGAGT",13643,13666,24,1949]
["forward primer 8","AGGGACATCAACGACGAAACAAGC",13738,13761,24]
["reverse primer 8","GGAGGAGTGGCATCAATGACTGGG",15546,15569,24,1831]
["forward primer 9","TGCCTATTTGCAGGAAACCAAGGC",15603,15626,24]
["reverse primer 9","TGAGCTGTCCGCCAGGAGCA",17403,17422,20,1819]
["forward primer 10","CGTCGCCTACCTGGCTATCAGGA",17494,17516,23]
["reverse primer 10","CCCTTTGGTTGGGGCTTTAGGA",19272,19293,22,1799]
["forward primer 11","GGTACTCTCATTAGAGGGTGCCGT",19314,19337,24]
["reverse primer 11","CCTGTCGTTTCAGGGCTGGCG",21095,21115,21,1801]
["forward primer 12","ACCCACGTGAGTTTGTGGATGCC",21160,21182,23]
["reverse primer 12","ACGATGATTGCCGTCTGGAGCC",22989,23010,22,1850]
["forward primer 13","AGAGGCAAAGGCTGCTTGCT",23204,23223,20]
["reverse primer 13","AGCTTCAGCATCCGTAACAGCTTCC",24979,25003,25,1799]
["forward primer 14","CGATGCACAAAAGACGGAACA",25012,25032,21]
["reverse primer 14","AGCAATTAATGTGGGCGACG",26865,26884,20,1872]
["forward primer 15","CCATTGCACTTGCAGGAACTACC",26996,27018,23]
["reverse primer 15","TGCTGTCACAGAGCCATCCTTGT",28776,28798,23,1802]
["forward primer 16","AACCACATGCTTCCAGCGGT",28992,29011,20]
["reverse primer 16","ACATGTCCATTGGCTGTTCTCCCA",30769,30792,24,1800]
["forward primer 17","ACGAACCAACAGGGGCAACGTC",30965,30986,22]
["reverse primer 17","CCACGCCGACGCATGGTTTT",32771,32790,20,1825]
["forward primer 18","GGGTTAATGCGGTTGCAGAAGCC",32904,32926,23]
["reverse primer 18","ACAGCTTGTGTTAGGTCTGCTGG",34688,34710,23,1806]
["forward primer 19","AGCATTGCTTGAAAACGAGT",34811,34830,20]
["reverse primer 19","CCTGCAAGAGCGCCTCCCAT",36595,36614,20,1803]
["forward primer 21","GCCAAGTACAAAACTGGACTTTACC",38854,38878,25]
["reverse primer 21","GTAAATGTCCATTCGTGTCTCTAAAGC",40823,40849,27,1995]
["forward primer 22","TTTGTCCGCTTACTGCAAGA",41616,41635,20]
["reverse primer 22","AGCGTTGCCCCATTTTGGGA",43414,43433,20,1817]
["forward primer 23","ACCAGGCAACAACATGGTCTCA",43520,43541,22]
["reverse primer 23","CGCCGACTCTTGCTCCTGGC",45312,45331,20,1811]
["forward primer 24","TTGCGGATGGTGGTTGGGCG",45467,45486,20]
["reverse primer 24","ACTGTTCGGCCAGTTCCTCA",47287,47306,20,1839]
["forward primer 25","TGGATTTCGTGCCTGGTTAGCCC",47410,47432,23]
["reverse primer 25","ACGGCCTTCACAACAGTAGGT",49262,49282,21,1872]
["forward primer 26","TCGTGAATTGCTTAAAGAAAAGGAAGCGT",49292,49320,29]
["reverse primer 26","AGGCGGAAGGTGTTAGCGCAC",50371,50391,21,1099]
[]
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["Primer start and end sites refer to nucleotide positions along the length of the plasmid (starting with and attL and ending with attR)."]