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["Additional File 10. Construction of promoter fusions for selected transcription-factor genes."]
[]
["AGI","5' flanking region (bp) 1","Forward primer (5'-3') 2","Reverse primer (5'-3') 2","Cloning method","Vector"]
["AT1G06160","-2304 to +15","AAAAGGGGTACCCACTGTCGCTGAAACGTCC","CTCTCTCGCGGATCCAGTTTGATATTCCATTTTCGATC","KpnI / BamHI","pBI-n1GFP"]
["AT1G28160","-1982 to +33","GTCAGTCCCAAGCTTCGACTTACGGTGTATTCCATG","GAGAGCTCTAGAAGAACCGGCATTCAAATTACCA","HindIII / XbaI","pBI-n1GFP"]
["AT1G35520","-1683 to +39","CATGCAGTGAAGCTTCTCATCAAACCAATTGACCC","CCTCTGAGCTCTAGATAATTCAGGTTGTGCATTCAC","HindIII / XbaI","pBI-n1GFP"]
["AT1G43160","-2113 to +36","GACTGAGTGAAGCTTGGGACGATATACTACGAGAG","GACTCGGGATCCCTCACCAGAGACAACATTAGT","HindIII / BamHI","pBI-n1GFP"]
["AT1G49770","-2009 to +18","TGATTACGCCAAGCTTGTGTTACGTTGTAACGAATTT","TGCTCACCATGGATCCCTCTTGAGCATTAGTCATATTG","HindIII / BamHI","pBI-GFP[S65T]"]
["AT1G55600","-2050 to +9","TGATTACGCCAAGCTTAATGTAAGAGTTTACTAAGC","TGCTCACCATGGATCCATCACTCATTTTTGACAAATC","HindIII / BamHI","pBI-n1GFP"]
["AT1G60280","-1317 to +15","TGATTACGCCAAGCTTCGTCGTCGCCATGTATAAAC","TGCTCACCATGGATCCGTCTTCAACTTTCATTGGTTCG","HindIII / BamHI","pBI-n1GFP"]
["AT1G67030","-2658 to +12","AAACGGGGTACCGTACTAATACGAGAATTGGGCA","TCAGGCTCTAGATTCAGTCGCCATATATGTGAGC","KpnI / XbaI","pBI-n2GFP"]
["AT1G75250","-2018 to +18","TGATTACGCCCTGCAGTATAAAAAAACACATGGAGA","TGCTCACCATGGATCCTCTTGAGTTTGACGCCATTAAT","PstI 3/ BamHI","pBI-GFP[S65T]"]
["AT1G75490","-1434 to +15","TATTCACGCGTCGACTGCTGTGTCACCTGGATTGC","TGCTCACCATGGATCCCTCTATGGATGACATGTACGGATG","SalI / BamHI","pBI-n1GFP"]
["AT2G22750","-1921 to +18","TGATTACGCCTGCAGCCCTAGCCAATCACATTC","TGCTCACCATTCTAGATCCGACGAGTGAGTTCATGT","PstI 3/ XbaI","pBI-GFP[S65T]"]
["AT2G33710","-1976 to +36","GACTGAGAGAAGCTTCTCCGTAACTCAATCTCAGC","GTCAGTCGGGATCCTGATTCTGGTGATAGAGGTCT","HindIII / BamHI","pBI-n1GFP"]
["AT2G40220","-2557 to +15","ATATGGGGTACCGGCAATTGGGTTGAGATCTC","GAGACGGGATCCAGCTAAAGGGTCCATAGATG","KpnI / BamHI","pBI-n1GFP"]
["AT3G01030","-896 to +15","ATATGGGGTACCGCAAGCAAAGACTAATGCTG","ATATCGGGATCCGCCAGTCTTCTTCATGAGAT","KpnI / BamHI","pBI-n2GFP"]
["AT5G01380","-1318 to +9","TGCTCATCCGCTCGAGCCATTTCAGAACAAGAGTAC","TGCTCACCATGGATCCTCGGTCCATTATTTGGAATCG","XhoI / BamHI","pBI-n1GFP"]
["AT5G01860","-1453 to +18","AAACGGGGTACCTTAGGTACAAGAAGGGGTCTTC","AAACGCGGATCCAGAGAGGTTTAGTTCCATAGTC","KpnI / BamHI","pBI-1GFPB, pBI-n1GFP, pBI-n2GFP 4"]
["AT5G11050","-1487 to +10","TGATTACGCCAAGCTTTGCTCTGTTAGTTGATTTC ","TGCTCACCATGGATCGTTCTTCCATGGAAGAAAAAG","In-Fusion","pBI-GFP[S65T]"]
["AT5G15830","-1955 to +39","CTTACTCCGCTCGAGGACCAACTAAGAGATAACACTCG","GACTCGGGATCCCATGTTGTTAAGACTTGAGCTATC","XhoI / BamHI","pBI-n1GFP"]
["AT5G27880","-2059 to +12","AAACGGGGTACCCTGAATGTGCAAACAAACATGTC","AAACGCGGATCCTGAAGAAGACATAGCTGGTTCTC","KpnI / BamHI","pBI-1GFPB, pBI-n1GFP, pBI-n2GFP 4"]
["AT5G41090","-1960 to +15","CTGAAGGGGTACCTCAAGCTTCTCTCTCAGTTG","AAAACGGGATCCGTATGTTGGGCCCATTATTC","KpnI / BamHI","pBI-n2GFP"]
["AT5G45980","-1370 to +12","TGATTACGCCAAGCTTTCAAACAAGAAAGATGCTTGG","TGCTCACCATGGATCCTGAGGAGGACATGATGATGG","HindIII / BamHI","pBI-n1GFP"]
["AT5G50490","-960 to +18","GAGTGACCCAAGCTTTGAAGTGCATTGGTTCGA","GTGTCGGGATCCGTTGTTGTTGTTCTCCATTTT","HindIII / BamHI","pBI-n1GFP"]
["AT5G53980","-1245 to +15","TGATTACGCCAAGCTTTCTTGAAACTTCGTGTAGC","TGCTCACCATGGATCCCTGAGAATTCTCCATTTTTCTC","HindIII / BamHI","pBI-n1GFP"]
["AT5G56200","-2167 to +18","CACACGGAAAAGCTTGTGGCCCTCCACTACTAAG","GACTCGGGATCCACCTCGTAATTGGTCCATCG","HindIII / BamHI","pBI-n1GFP"]
[]
["    The 5' upstream sequences of 24 transcription-factor genes were amplified using the forward and reverse primer, and cloned into the designated vectors through restriction digestion or In-Fusion technology (BD Biosciences) as described in Methods. The AGI numbers are listed in ascending order.\n   1 Positions are relative to the predicted translation start codon. \n   2 Underlined sequences are restriction sites or vector sequences for homologous recombination through In-Fusion technology.\n   3 The PCR product was digested with PstI, while the binary vector was digested with SbfI (compatible with PstI). \n   4 The amplified PCR product was first cloned into pBI-n2GFP, sequenced, then subcloned into pBI-n1GFP or pBI-1GFPB."]