{"text_format":"3","workbook":"xls","n_sheets":1,"sheets":["Additional File 3"],"not_rendered":{"external_links":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

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["Additional File 3. Secondary qRT-PCR screen of selected transcription-factor genes."]
[]
[{"$nulls":5},"RNA source: e",null,null,"RNA source: f",null,null,"RNA source: g"]
["AGI","Forward primer (5'-3')","Reverse Primer (5'-3')","Amplicon size (bp)",null,"Normalized CT, ms1","Normalized CT, dif1",null,"Normalized CT, ms1","Normalized CT, dif1",null,"Normalized CT, ms1","Normalized CT, dif1",null,"Average CT, ms1","Average DDCT","P (t-test)"]
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["AT4G38070",{"$nulls":4},{"v":32.6277388594766,"d":"32.63"},{"v":35.5737111476478,"d":"35.57"},null,{"v":33.498584633080796,"d":"33.50"},{"v":34.8564312846387,"d":"34.86"},null,{"v":33.6565714224181,"d":"33.66"},{"v":36,"d":"36.00"},null,{"v":33.260964971658495,"d":"33.26"},{"v":2.2157491724370053,"d":"2.22"},{"v":0.020488335346876857,"d":"0.020"}]
["AT5G01380",{"$nulls":4},{"v":28.164763713881502,"d":"28.16"},{"v":28.924421698400003,"d":"28.92"},null,{"v":27.9099113199244,"d":"27.91"},{"v":27.2785923483967,"d":"27.28"},null,{"v":27.8262973604378,"d":"27.83"},{"v":27.891562571238097,"d":"27.89"},null,{"v":27.96699079808123,"d":"27.97"},{"v":0.06453474126370295,"d":"0.06"}]
["AT5G01860",{"$nulls":4},{"v":28.6137712701304,"d":"28.61"},{"v":31.823799118813803,"d":"31.82"},null,{"v":28.7185944727064,"d":"28.72"},{"v":31.6430260002412,"d":"31.64"},null,{"v":29.1511390617469,"d":"29.15"},{"v":31.6647209762667,"d":"31.66"},null,{"v":28.827834934861233,"d":"28.83"},{"v":2.8826804302460034,"d":"2.88"},{"v":0.002440278582839377,"d":"0.002"}]
["AT5G11050",{"$nulls":4},{"v":27.4933521521564,"d":"27.49"},{"v":31.404688000822098,"d":"31.40"},null,{"v":27.229088991937903,"d":"27.23"},{"v":31.698938292230597,"d":"31.70"},null,{"v":27.716175195338998,"d":"27.72"},{"v":31.8752810894286,"d":"31.88"},null,{"v":27.479538779811104,"d":"27.48"},{"v":4.1800970143493315,"d":"4.18"},{"v":0.0007453287756822681,"d":"0.001"}]
["AT5G15830",{"$nulls":4},{"v":34.0976769308366,"d":"34.10"},{"v":33.816082366524,"d":"33.82"},null,{"v":36,"d":"36.00"},{"v":35.037825417509694,"d":"35.04"},null,{"v":34.8741098995028,"d":"34.87"},{"v":34.0357722188004,"d":"34.04"},null,{"v":34.99059561011313,"d":"34.99"},{"v":-0.6940356091684331,"d":"-0.69"}]
["AT5G27880",{"$nulls":4},{"v":28.9162687584943,"d":"28.92"},{"v":36,"d":"36.00"},null,{"v":29.1194692704205,"d":"29.12"},{"v":35.4781055987589,"d":"35.48"},null,{"v":28.841176252870298,"d":"28.84"},{"v":35.27671825369269,"d":"35.28"},null,{"v":28.9589714272617,"d":"28.96"},{"v":6.625969856888834,"d":"6.63"},{"v":0.0006011357790074573,"d":"0.001"}]
["AT5G35550","AAGAATCATTGGAACTCAAACCTC","CATACATTATTCTCGTTGTTCGTCG",101,null,{"v":29.355296084617198,"d":"29.36"},{"v":30.7902405717234,"d":"30.79"},null,{"v":28.7364087698101,"d":"28.74"},{"v":28.3836500168824,"d":"28.38"},null,{"v":28.5846886027733,"d":"28.58"},{"v":28.9166941641032,"d":"28.92"},null,{"v":28.8921311524002,"d":"28.89"},{"v":0.47139709850279843,"d":"0.47"}]
["AT5G39700","AGAGGAGAAGAAGAAGAAACATAGG","TAATCTTCGGATCGAGCTGGTTAAA",179,null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":0,"d":"0.00"}]
["AT5G41090",{"$nulls":4},{"v":26.5390477107475,"d":"26.54"},{"v":30.0472632338897,"d":"30.05"},null,{"v":26.0250998111884,"d":"26.03"},{"v":30.3174088344657,"d":"30.32"},null,{"v":26.4023095098471,"d":"26.40"},{"v":30.0205474978135,"d":"30.02"},null,{"v":26.322152343927666,"d":"26.32"},{"v":3.806254178128635,"d":"3.81"},{"v":0.0020603874992002873,"d":"0.002"}]
["AT5G41200",{"$nulls":4},{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":0,"d":"0.00"}]
["AT5G42640","GTGTAACGGTGTGTTTGATACTT","TATTTCTTGCACGAAATCTTTGGT",107,null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":0,"d":"0.00"}]
["AT5G45980",{"$nulls":4},{"v":32.8137085850955,"d":"32.81"},{"v":35.561493153046,"d":"35.56"},null,{"v":33.728101964677606,"d":"33.73"},{"v":35.6061350980926,"d":"35.61"},null,{"v":33.738017138362196,"d":"33.74"},{"v":36,"d":"36.00"},null,{"v":33.426609229378435,"d":"33.43"},{"v":2.2959335210011034,"d":"2.30"},{"v":0.005900650932510329,"d":"0.006"}]
["AT5G50490",{"$nulls":4},{"v":35.7331752793411,"d":"35.73"},{"v":34.9162882210675,"d":"34.92"},null,{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":34.7825712875516,"d":"34.78"},{"v":34.8033362651301,"d":"34.80"},null,{"v":35.505248855630896,"d":"35.51"},{"v":-0.265374026898364,"d":"-0.27"}]
["AT5G53980",{"$nulls":4},{"v":36,"d":"36.00"},{"v":35.1752756353449,"d":"35.18"},null,{"v":36,"d":"36.00"},{"v":35.1733947491989,"d":"35.17"},null,{"v":35.7045563588694,"d":"35.70"},{"v":34.3546716680481,"d":"34.35"},null,{"v":35.9015187862898,"d":"35.90"},{"v":-1.000404768759168,"d":"-1.00"}]
["AT5G54070","TTTGGTGAACAATCAAAGCGG","TTGCTCTACTCTATCTCTATCCTCC",111,null,{"v":32.111082868451696,"d":"32.11"},{"v":36,"d":"36.00"},null,{"v":30.9150805837705,"d":"30.92"},{"v":35.6775423324257,"d":"35.68"},null,{"v":31.9092890874541,"d":"31.91"},{"v":36,"d":"36.00"},null,{"v":31.645150846558764,"d":"31.65"},{"v":4.247363264249803,"d":"4.25"},{"v":0.0019213691212951278,"d":"0.002"}]
["AT5G55690",{"$nulls":4},{"v":34.8556465095008,"d":"34.86"},{"v":31.0541386822891,"d":"31.05"},null,{"v":35.1888905454543,"d":"35.19"},{"v":34.50088974107881,"d":"34.50"},null,{"v":36,"d":"36.00"},{"v":33.8917266158637,"d":"33.89"},null,{"v":35.34817901831837,"d":"35.35"},{"v":-2.199260671907832,"d":"-2.20"}]
["AT5G56200","TCATCCACTTGGTAATTCATCGTC","TCCAATTCCAAATTACTGTCCCAT",150,null,{"v":32.5123969435197,"d":"32.51"},{"v":36,"d":"36.00"},null,{"v":31.6435659058166,"d":"31.64"},{"v":33.6125444173024,"d":"33.61"},null,{"v":31.9396365154973,"d":"31.94"},{"v":34.3535011921622,"d":"34.35"},null,{"v":32.031866454944534,"d":"32.03"},{"v":2.6234820815436706,"d":"2.62"},{"v":0.014136594643261264,"d":"0.014"}]
["AT5G58010",{"$nulls":4},{"v":36,"d":"36.00"},{"v":36,"d":"36.00"},null,{"v":34.8648532700023,"d":"34.86"},{"v":36,"d":"36.00"},null,{"v":36,"d":"36.00"},{"v":34.7782291100793,"d":"34.78"},null,{"v":35.62161775666743,"d":"35.62"},{"v":-0.028874719974330294,"d":"-0.03"}]
["AT5G58850",{"$nulls":4},{"v":27.7016326995362,"d":"27.70"},{"v":30.4010523543786,"d":"30.40"},null,{"v":27.579999840311903,"d":"27.58"},{"v":28.974908020501797,"d":"28.97"},null,{"v":27.9201852306164,"d":"27.92"},{"v":30.503506386261503,"d":"30.50"},null,{"v":27.7339392568215,"d":"27.73"},{"v":2.2258829968924645,"d":"2.23"},{"v":0.016663080789203536,"d":"0.017"}]
["AT5G59780","AGAGAAGAAGCGACCTATGT","CCGTCTTCGCATTCTTGATT",126,null,{"v":31.046179837064702,"d":"31.05"},{"v":28.9931956889791,"d":"28.99"},null,{"v":30.5998432196253,"d":"30.60"},{"v":30.5329699690932,"d":"30.53"},null,{"v":31.2026590883991,"d":"31.20"},{"v":30.5307612811024,"d":"30.53"},null,{"v":30.9495607150297,"d":"30.95"},{"v":-0.9305850686381317,"d":"-0.93"}]
["AT5G61890",{"$nulls":4},{"v":29.0458981939951,"d":"29.05"},{"v":33.0985753733857,"d":"33.10"},null,{"v":28.864417253692302,"d":"28.86"},{"v":31.136729872485898,"d":"31.14"},null,{"v":29.0625338331994,"d":"29.06"},{"v":31.9379243395373,"d":"31.94"},null,{"v":28.990949760295603,"d":"28.99"},{"v":3.066793434840701,"d":"3.07"},{"v":0.013925074396310848,"d":"0.014"}]
[]
["        The mRNA levels of 69 transcription-factor genes identified in the primary qRT-PCR screen (DDCT >1.5; Additional File 1) were analyzed using three additional pairs of ms1-dif1 RNAs (e-g; Additional File 9). The AGI numbers are listed in ascending order along with the sequence and amplicon size of the 17 primer-pairs re-designed for the secondary qRT-PCR screen (the remaining primers were as described in Additional File 1). Normalized CT values were calculated as described in Methods. A one-tailed, paired Student's t-test was performed for genes with average DDCT values above 1.0. Significant P-values (a = 0.10) are in bold. The AGI numbers of the 24 genes subsequently analyzed using promoter fusions (Additional File 5) are highlighted in yellow."]