{"text_format":"3","workbook":"xls","n_sheets":2,"sheets":["Primers","Log2 ratio"],"not_rendered":{"charts":1,"external_links":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

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["Additional file 10. Primers used for qPCR experiments, and comparison of results obtained by microarray and qPCR analysis for selected genes. In the included graph, each point represents the Log2-ratio of the mRNA abundance determined by microarray in copper stress and in control conditions for a specific gene after 4 h or 8 h of Cu treatment (mean of three biological replicates) plotted against the same ratio determined by qPCR."]
[]
["Primer name","Primer sequence","Length","Tm (°C)"]
["Esi0096_0043_GSDH_F","GCAACAACTACGGGTGGTC",19,"55.5"]
["Esi0096_0043_GSDH_R","GAAGAGCGGGAAGGTGAAG",19,"54.6"]
["Esi0355_0025_HSP70_F","CCGAGGACTACCGTGAGG",18,"54.9"]
["Esi0355_0025_HSP70_R","CTCCGCCATCATCTTCCAG",19,"54.4"]
["Esi0279_0021_ HSF_F","CTAGTTCGCCTGCTTCGTC",19,"55.1"]
["Esi0279_0021_ HSF_R","GTCGCTGTTGTTCATACTTCTC",22,55]
[" Esi0060_0078_AOS_F","GTGATCTCTCCTGACGAGTTTC",22,"55.7"]
[" Esi0060_0078_AOS_R","TCCCGCTTACATCTTCTTACG",21,"54.8"]
[" Esi0085_0005_e/unk_F","CAGTGAGTGCCCCTAAACAG",20,"55.2"]
[" Esi0085_0005_e/unk_R","ATGGAGCAACTTCGTTCGG",19,"54.9"]
[" Esi0249_0028_NIR_F","GGGCTCCGAGAACAACTTC",19,"54.9"]
[" Esi0249_0028_NIR_R","AGACAGACAGGCAGGTAGG",19,"54.7"]
[" Esi0163_0024_ChTr_F","ACCGTCCTCTCCCTGTTTC",19,"55.3"]
[" Esi0163_0024_ChTr_R","CCTCCTTTCACTCTATCCTTCG",22,"55.2"]
[" Esi0028_0164_GDH_F","TTGCCTTCTTTGTCCCAGAG",20,"54.7"]
[" Esi0028_0164_GDH_R","GTGCTATGTGCCCAGTGC",18,"55.2"]
[" Esi0058_0142_FeR_F","CGAGTTGTAGGTGCTCAAATTC",22,"55.2"]
[" Esi0058_0142_FeR_R","AAACGATAGCGGGATACCAC",20,"54.5"]
[" Esi0184_0026_L23A_F","CTGACGGCAAGAAGAAGGC",19,"55.6"]
[" Esi0184_0026_L23A_R","GGAATGAATGAAAGGGCAACG",21,"55.4"]
[" Esi0025_0138_GDPF_F","TTGAAGTAGAAACCCTCCGAAG",22,"55.2"]
[" Esi0025_0138_GDPF_R","CAACACAAGTCAGCCAACAC",20,"54.9"]
[" Esi0161_0055_ZnFePer_F","GTTAGGGTCGTTCGTGCTG",19,"55.4"]
[" Esi0161_0055_ZnFePer_R","AATTGTGCCTGCGTGTGG",18,"55.4"]
[" Esi0348_0030_AIP-1-2_F","CTTGGGTTGTGGAGAGAGTC",20,"54.5"]
[" Esi0348_0030_AIP-1-2_R","AAGCACGCATAGGATCACC",19,"54.3"]
[" Esi0513_0010_MTF1_F","AAAGAGGCACCAACGACTAC",20,"54.8"]
[" Esi0513_0010_MTF1_R","TCACAACGACTTGACACAGG",20,"55.1"]
[" Esi0290_0037_e/unk_F","TGACGGCAGTGTTTGACG",18,"54.8"]
[" Esi0290_0037_e/unk_R","TGCTCGCCAGGTATCTCTAC",20,"55.7"]
[" Esi0495_0003_MEP6_F","TGTGATGTGGAGTGGTTGTC",20,"54.7"]
[" Esi0495_0003_MEP6_R","ATACTCTCAGAACACCGCTTG",21,55]
[" Esi0043_0144_TPR-1_F","TGGCGTCAGAGTGTTGTTG",19,"55.1"]
[" Esi0043_0144_TPR-1_R","CACAGCAGTCCCTTCTTATCC",21,"55.3"]
[" Esi0010_0167_TYR_F","TTGTGTGTTGCCGACTGAG",19,"55.1"]
[" Esi0010_0167_TYR_R","CCGCCGCATTGATTGTTTG",19,"55.4"]
["Esi0387_0021_EF1alpha_F","GCAAGGGCCTCAGCTCTG",19,60.6]
["Esi0387_0021_EF1alpha_R","ACAAGCCGTCTGGGTATATGTTAGC",26,62.4]
["Esi0054_0059_UBCE_F","AACAATGGCCTTTGCGAAAA",21,61.27]
["Esi0054_0059_UBCE_R","GCGTACGTCTTGAAGCCCAG",21,61.68]

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["Microarray probe ID","Genome ID","Short name","4h (mean ± SD)",{"$nulls":3},"8h (mean ± SD)"]
[{"$nulls":3},"qPCR",null,"Microarrays",null,"qPCR",null,"Microarrays",null,"Function"]
["CL1656Contig1","Esi0028_0164","GDH",{"v":-2.4024683864114187,"d":"-2.4"},{"v":0.30980448445901,"d":"± 0.3"},{"v":-2.9960244486666676,"d":"-3.0"},{"v":0.3251882346930929,"d":"± 0.3"},{"v":-1.6284402597285725,"d":"-1.6"},{"v":1.0733687298131795,"d":"± 1.1"},{"v":-1.4958994626666673,"d":"-1.5"},{"v":0.6127879893421119,"d":"± 0.6"},"Glutamate dehydrogenase 1 (NADP-dependent)"]
["CL416Contig1","Esi0010_0167","TYR",{"v":0.08593471658528169,"d":"0.1"},{"v":0.07684699695642826,"d":"± 0.1"},{"v":-2.6383898346666683,"d":"-2.6"},{"v":0.7064379968985344,"d":"± 0.7"},{"v":-0.02694476436154854,"d":"-0.0"},{"v":0.19176885333220584,"d":"± 0.2"},{"v":-1.519613304,"d":"-1.5"},{"v":0.6749192590189892,"d":"± 0.7"},"Putative Tyrosinase-like protein"]
["CL2999Contig1","Esi0249_0028","NIR",{"v":-3.003312292388999,"d":"-3.0"},{"v":0.4616300920590576,"d":"± 0.5"},{"v":-2.135849693333334,"d":"-2.1"},{"v":0.03417487337918811,"d":"± 0.0"},{"v":-2.8315301407105298,"d":"-2.8"},{"v":1.4866660251539918,"d":"± 1.5"},{"v":-1.8531924833333324,"d":"-1.9"},{"v":0.07005947886362893,"d":"± 0.1"},"Nitrite reductase (NAD(P)H) large subunit"]
["CL6178Contig1","Esi0058_0142","FerR",{"v":-1.1886955385378448,"d":"-1.2"},{"v":0.25191382845432797,"d":"± 0.3"},{"v":-1.8817570120000005,"d":"-1.9"},{"v":0.3660884263416132,"d":"± 0.4"},{"v":-0.6902964827460077,"d":"-0.7"},{"v":0.33365299407592686,"d":"± 0.3"},{"v":-0.5185468599999998,"d":"-0.5"},{"v":0.27437425577225094,"d":"± 0.3"},"Ferric chelate reductase"]
["LQ0AAB7YM14FM1,SCF","Esi0163_0024","ChTr",{"v":-3.0399624363949473,"d":"-3.0"},{"v":0.05189516234053258,"d":"± 0.1"},{"v":-1.5211049933333332,"d":"-1.5"},{"v":0.07892976353483827,"d":"± 0.1"},{"v":-1.0621404345179972,"d":"-1.1"},{"v":0.7396622110947558,"d":"± 0.7"},{"v":-0.5760900600000003,"d":"-0.6"},{"v":0.3742745408139321,"d":"± 0.4"},"Chromate transporter region"]
["LQ0AAB22YJ14FM1,SCF","Esi0025_0138","GDF",{"v":-0.08551285875096348,"d":"-0.1"},{"v":0.6376022472887801,"d":"± 0.6"},{"v":-0.0006579106666668721,"d":"-0.0"},{"v":0.5759540549459007,"d":"± 0.6"},{"v":-1.0165292487924804,"d":"-1.0"},{"v":0.7431432005510847,"d":"± 0.7"},{"v":-0.2525635083333331,"d":"-0.3"},{"v":0.5946107756969948,"d":"± 0.6"},"GDP-fucose protein O-fucosyltransferase"]
["CL1795Contig1","Esi0290_0037 ","n/a",{"v":-0.44215330225425936,"d":"-0.4"},{"v":0.08101888703291417,"d":"± 0.1"},{"v":0.000256126666666745,"d":"0.0"},{"v":0.014726485924832352,"d":"± 0.0"},{"v":-0.18346726071343175,"d":"-0.2"},{"v":0.057581593157769145,"d":"± 0.1"},{"v":0.08650795666666866,"d":"0.1"},{"v":0.012579629373675362,"d":"± 0.0"},"Expressed unknown protein"]
["CL4814Contig1","Esi0184_0026","LA23",{"v":-0.1928132386433045,"d":"-0.2"},{"v":0.04794515528343752,"d":"± 0.0"},{"v":0.005301809999998852,"d":"0.0"},{"v":0.10368729165065596,"d":"± 0.1"},{"v":-0.0771429250866054,"d":"-0.1"},{"v":0.479906112955665,"d":"± 0.5"},{"v":0.06893606666666585,"d":"0.1"},{"v":0.2427979397742864,"d":"± 0.2"},"60S RIBOSOMAL PROTEIN L23A"]
["CL2465Contig1","Esi0348_0030","AIP",{"v":1.390160930877378,"d":"1.4"},{"v":0.0312723569065204,"d":"± 0.0"},{"v":1.108804489333333,"d":"1.1"},{"v":0.26663451860927445,"d":"± 0.3"},{"v":0.6935309317136261,"d":"0.7"},{"v":0.37493258377718836,"d":"± 0.4"},{"v":0.6953072413333325,"d":"0.7"},{"v":0.2896383594816112,"d":"± 0.3"},"Zinc finger AN1 and C2H2 domain-containing Arsenate stress-associated protein 1"]
["LQ0AAB14YG13FM1,SCF","Esi0161_0055","ZFP",{"v":-0.09740293060773836,"d":"-0.1"},{"v":0.48005554983250714,"d":"± 0.5"},{"v":1.108804489333333,"d":"1.1"},{"v":0.3087891167085086,"d":"± 0.3"},{"v":-0.7160236838064779,"d":"-0.7"},{"v":0.3865002203353389,"d":"± 0.4"},{"v":0.6953072413333325,"d":"0.7"},{"v":1.361066651180639,"d":"± 1.4"},"Putative metal ion transport, Zinc/iron permease"]
["CL4138Contig1","Esi0513_0010","MTF1",{"v":1.4117717227382238,"d":"1.4"},{"v":0.26427583896544266,"d":"± 0.3"},{"v":1.4235347066666666,"d":"1.4"},{"v":0.1583802384859293,"d":"± 0.2"},{"v":0.793177330756755,"d":"0.8"},{"v":0.5000766205774121,"d":"± 0.5"},{"v":0.8315649166666663,"d":"0.8"},{"v":0.40597155933011303,"d":"± 0.4"},"Similar to metal response element-binding transcription factor-1."]
["LQ0AAB60YG14FM1,SCF","Esi0043_0144","TPR1",{"v":4.208447832765907,"d":"4.2"},{"v":0.050082077325645846,"d":"± 0.1"},{"v":4.313269410666666,"d":"4.3"},{"v":0.3973092261732013,"d":"± 0.4"},{"v":3.376852825368813,"d":"3.4"},{"v":1.679555135480118,"d":"± 1.7"},{"v":3.0531753393333343,"d":"3.1"},{"v":1.5620924651734713,"d":"± 1.6"},"Tetratricopeptide domain containing protein"]
["CL1509Contig1","Esi0085_0005","n/a",{"v":4.369709879132151,"d":"4.4"},{"v":0.17409327555085294,"d":"± 0.2"},{"v":4.688324987666667,"d":"4.7"},{"v":0.07959778314874819,"d":"± 0.1"},{"v":2.164247832770841,"d":"2.2"},{"v":1.4817170745488988,"d":"± 1.5"},{"v":3.8337277083333348,"d":"3.8"},{"v":0.12955690738326459,"d":"± 0.1"},"Expressed unknown protein"]
["LQ0AEA5YO08RM1,SCF","Esi0495_0003","MEP6",{"v":5.889012453494613,"d":"5.9"},{"v":0.2095531744390073,"d":"± 0.2"},{"v":5.013889245666668,"d":"5.0"},{"v":0.500112785919153,"d":"± 0.5"},{"v":3.3867078893428957,"d":"3.4"},{"v":1.6853978935356513,"d":"± 1.7"},{"v":3.561251018666667,"d":"3.6"},{"v":1.956009930183628,"d":"± 2.0"},"Mannuronan C-5-epimerase"]
["CL3423Contig1","Esi0060_0078","AOS",{"v":3.193755530785347,"d":"3.2"},{"v":0.7245608641599399,"d":"± 0.7"},{"v":5.3699791019999985,"d":"5.4"},{"v":0.3158130251044346,"d":"± 0.3"},{"v":1.744740632063956,"d":"1.7"},{"v":1.5616446911622708,"d":"± 1.6"},{"v":2.924526359333335,"d":"2.9"},{"v":1.45550733824163,"d":"± 1.5"},"CYP5164A2"]
["CL7212Contig1","Esi0096_0043","GSDH",{"v":4.366290945755032,"d":"4.4"},{"v":0.33197086034453727,"d":"± 0.3"},{"v":5.832317442333332,"d":"5.8"},{"v":0.37070133810670886,"d":"± 0.4"},{"v":4.82158093207543,"d":"4.8"},{"v":2.489237854407293,"d":"± 2.5"},{"v":2.397919491333333,"d":"2.4"},{"v":1.3448507054131495,"d":"± 1.3"},"Glucose sorbosone dehydrogenase"]