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["Additional files: Table S1. Information of 16 SSR makers used to study the population genetics of C. simplicipinna."]
["Primers code","Dyes","Original code","Primer sequences","Annealing temperature"]
[{"$nulls":4},"（℃）","NA","NE","I","Ho","HE","F"]
["A1","6-FAM","Cy-Tai EST-SSR01 [27]","F:CGAGAAGTAATTTGCAAATGC",55,4,2.34,0.966,0.308,0.573,0.463]
[{"$nulls":3},"R:TGTGAAGCTAAATAGTTGGG"]
["A2","HEX","G46 [28]","F:CAAAAGCCCCTTGAAACC",55,3,1.467,0.595,0.253,0.318,0.206]
[{"$nulls":3},"R:AAGCTCGACTTGTAGATGGA"]
["A4","ROX","Cy-Tai EST-SSR05 [27]","F:AACAGACCATGAGGACCAGG",55,2,1.056,0.126,0.055,0.053,-0.028]
[{"$nulls":3},"R:GCTGGTATTCCTTAATGCAC"]
["A5","6-FAM","Cy-Tai EST-SSR08 [27]","F:GAAAATGCTTTGATGTTCCC",60,16,11.967,2.596,0.67,0.916,0.269]
[{"$nulls":3},"R:TGGGCCAACTTTAAGCACAC"]
["A7","HEX","CY232 [33]","F:TCTTGCTTACCCGTTTGCTT",55,5,2.018,0.959,0.242,0.505,0.521]
[{"$nulls":3},"R:CTCCTCGACGTTCAATCACA"]
["A8","ROX","HL01 [32]","F:TCCTTTCATGGACACTCTTACG",48,2,1.971,0.686,0.352,0.493,0.286]
[{"$nulls":3},"R:ATTTATTAGATGTCTAGCTTA"]
["A10","HEX","HL08 [32]","F:AAAACATTCCTTGCCCTGT",56,6,1.98,0.899,0.374,0.495,0.245]
[{"$nulls":3},"R:GGAGCCTGTTGAAGAGCTA"]
["A11","6-FAM","Cha04 [30]","F:ACTCCTCACTCCCTCGC",64,23,9.035,2.588,0.495,0.889,0.444]
[{"$nulls":3},"R:CTAGCCTCTTGTTGCCATTAT"]
["A13","6-FAM","E004 [28]","F:CTATCATCAGAGCCTCATTC",54,11,3.992,1.793,0.308,0.749,0.589]
[{"$nulls":3},"R:AAGTCATACATGGACAGCAA"]
["A15","HEX","Cpz8 [29]","F:TGCTAAAGTGAACGACGAA",55,4,2.942,1.111,0.341,0.66,0.484]
[{"$nulls":3},"R:CATACTCCTTATCCCACGAAT"]
["A17","ROX","Cha05 [30]","F:GTCTGCTAACATCTATAAA",52,4,1.837,0.856,0.154,0.456,0.662]
[{"$nulls":3},"R:GATGAGCTAAGAGTCATAGTA"]
["A18","ROX","E001 [28]","F:TGGGATTAATATTCCAGAAA",52,6,4.83,1.644,0.736,0.793,0.071]
[{"$nulls":3},"R:CGACGAGTCTGATGTAGGTAT"]
["A19","ROX","Cha-estssr01 [31]","F:GATTCTTGCTCTGTTCGCTCAT",60,32,10.13,2.84,0.505,0.901,0.439]
[{"$nulls":3},"R:CAGAACCCCTGAACTGTCAAAC"]
["A21","HEX","HL02 [32]","F:GGGGTTCATATCACATAAC",50,22,12.425,2.756,0.802,0.92,0.128]
[{"$nulls":3},"R:CTATAAAGAATCATCGTTCTC"]
["A23","HEX","Cha-estssr02 [31]","F:ATAGGCTTCCTTTAGTGATGTC",50,6,3.635,1.412,0.231,0.725,0.682]
[{"$nulls":3},"R:GCCTTTAGTAGTATCGGATTA"]
["A24","ROX","Cha-estssr04 [31]","F:GATGTTCCCAAATAATGTTACA",54,15,11.43,2.556,0.692,0.913,0.241]
[{"$nulls":3},"R:CAAGCTGCACATGCAATGA"]
["Mean",{"$nulls":4},9.75,4.769,1.473,0.38,0.636,0.404]
["Total",{"$nulls":4},195,95.38]
["Note: NA, number of alleles; NE, effective number of alleles; I, information index; HO, observed heterozygosity; HE, expected heterozygosity; F, fixation index."]

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