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["Additional file 1. PCR primers and initial cycling conditions"]
["Primer ID","Fwd Primer","Rev Primer"]
["A","GGCGACCCAATGTTTCTCTA ","TTCTTTCGTCCACATCAGCA "]
["B","TGGCAGAGGACCTTCTGAGT ","AGGATAATGGTGGGGAGAGG"]
["C","GGAATGGAATGTCTCGGTGT","TGACCCAGGACTTGCAGTAA"]
["D","GCAGAAGCATTGCCAGTGTA ","CCGGTGACAGACAATAAGCA"]
["E","CCAGTGTCATCATCCAAACG","AGGTGGCATCCTTCAGACAC"]
["F","AGAAAGGGGAGGGCAGAATA","GGGAGAAGAAACAGGGAAGG"]
["GRE2","GGACAGGCATCTTATCTACCTTG","TTCAGACACTATAGGCCGTCTCT"]
["ARE1","CAGGCGATTCTCCACCTTG","GGTGGCCACTTACGAGTCTCCCA "]
["G ","CCCAACTTTGAGGCCAATAC","AAATACCACTGCACCCTCAGA"]
["H","GGGGTGGGGAGGATAAAAT ","GACATGCCTGGAATGCTTCT"]
["I","GAGTGGCCTATTCTTGCTAGTGA ","TCCCGGTAAGGTAGGGAATACTA"]
["J","AAACGTATTTACTGTGCCAACA","GCAAGACTGGGAGGAAAGTTAGT"]
["K","TCTCAACTTCTGGAGACCTTCTG","AACAAATGCCTGACTGCAATAAT"]
["L","CAGAGAAGCCAAGTGTCAACTTT","TCAGCATAAATTAGGAGTTGGGA  "]
["M","TTGCCCTTTCAATAGACAGTGTT  ","AGGTAGTATGCAGAGTGCTGAGG  "]
["N","ACTGCCTACCTAGCATCTCTCCT  ","TGGCATTAACTGCTATAGGATGC  "]
["O","CCTCAGCACTCTGCATACTACCT  ","TTCCTTTGGCAATATGTCACTTT  "]
["P","GAAGGGCAGTTATCTGTCTTGAA  ","GAGATGTGACAAATGTGGCATTA  "]
["Q","TCTGAACTATCAGAAGCAGTGCC  ","AAGGCATTCTAGGAATCAGGAAC  "]
["R","GGATCACAAGGTTAGGAGTTCAA  ","TCATAAAGCACCCTGACTCAGAT  "]
["S","GCTTAGCCCACCTTAAGTGCTAT  ","GAATCTAAACCCAACACATGCTC  "]
["T","ACCCAGAGGTACCTTGTATTTGG  ","AGAGCTGCCTTATGAAGAAGGAT  "]
["U","GGTACCAGCTAGAGGAGATAGGG  ","TCAGCTGGAGAGAGATGACTACC  "]
["V","CATCCACATTAGCAGTTGGTGTA  ","AGTTCTATGAGGGCAAGGACTTT  "]
["W","TTTATCTAACCCTTCCCTGATGG  ","CAAGGCCTTCAGGAATTAACTTT  "]
["X","GAAGGAGCAGTTAGTGCAAGAAA  ","AGCTTTGTCAACCATCAACAAAT  "]
["Y","CAATTCCATAAACACATCCTGGT  ","TGCCCTTTAGGACATAAATCTGA  "]
["Z","AAGAGCAGGGAAAGAGACCTTTA  ","CTTGGTCATGTGAGGATACAACA  "]
["AA","ACAGTCCCATGATGTTATGATCC  ","ATCACGAGGTCAAGAGTTTGAGA  "]
["BB","TAAGGTCACTAATCCCATTCACG  ","TTAGACTTGAACTCCTGTGCTCC  "]
["CC","CATGAGAGACAGGTCATTAAGCC  ","TGTTCACACAACAAAGAAATTGG  "]
["DD","TCCCTCTTACCTCTTATTCAGCC  ","AATTTGCATTGTTTCAAAGAGGA  "]
["EE","ACAACTTCAGAGGCAGTCTATGC  ","TTTAGGAGGCAGTACATCACCAT  "]
["FF","ATTGCTGCTGGTAGCTGTCTTAC  ","GCTGCTTCAGGATGTTATTATGG  "]
["GG","CAACAAATACTGCTGTTGTTCCA  ","ATATTTATTAACAGCAGGCCGGT  "]
[]
["All primer pairs were tested using the cycling conditions listed below.  The annealing tempurature was determined by gel electrophoresis.  "]
[]
["Cycling Conditions"]
["95oC for 10 min"]
["95oC for 30 sec"]
["Temp Gradient (50oC-62oC) for 30 sec"]
["72oC for 1 min"]
["Repeat steps 2-4 for 39 cycles"]
["72oC for 5 min"]

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