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["Gene","Mutation","Amplicon (bp)","PCR Primer","LH-G Probe† (Probe Name)"]
["EGFR","In-frame deletion",212,"F: GGACTCTGGATCCCAGAAGGTG;","GGACTCTGGA TCCCAGAAGG TGAGAAAGTT"]
[null,"in exon 19",null,"R: CATTTAGGATGTGGAGATGAGC","  AAAATTCCCG TCGCTATCAA GGAATTAAGA"]
[{"$nulls":4},"  GAAGCAACAT CTCCGAAAGC CAACAAGGAA"]
[{"$nulls":4},"  ATCCTCGAT (19JWTF)"]
["EGFR","In-frame insertion",189,"F: AAGCCACACTGACGTGCCTC","AAGCCACACT　GACGTGCCTC　TCCCTCCCTC　"]
[null,"in exon 20",null,"R: GTACTGGGAGCCAATATTGTCTTTG","CAGGAAGCCT　ACGTGATGGC　CAGCGTGGAC　"]
[{"$nulls":4},"AACCCCCACG　TGTGCCGCCT　GCTGGGCATC　"]
[{"$nulls":4},"TGCC "]
["EGFR","Point mutation in",161,"F: GGCATGAACTACTTGGAGGAC; ","CTTACTTTGC CTCCTTCTGC ATGGTATTCT"]
[null,"exon 21 at L858",null,"R: CTTACTTTGCCTCCTTCTGCATG","    TTCTCTTCCG CACCCAGCAG *******AGC"]
[{"$nulls":4},"    CCAAAATCTG TGATCTTGAC ATGCTGCG (E7R)"]
["K-ras","Point mutation",165,"F: AAGGCCTGCTGAAAATGACTG;","AAGGCCTGCT　GAAAATGACT　GAATATAAAC"]
[null,"in exon 2 at G12",null,"R: GGTCCTGCACCAGTAATATGCA","TTGTGGTAGT　TGGAGCTGGT　TCTGCAGAAG　"]
[{"$nulls":4},"GTGTAGGCAA　GAGTGCCTTG　ACGATACAG (IN4)"]
["bp, base pairs; EGFR, epidermal growth factor receptor; F, forward; LH-G, loop-hybrid generator; PCR, polymerase chain reaction; R, reverse."]
["†Asterisks indicate deleted nucleotides; underlined type, nucleotides for the hot spots of mutations to be detected."]
["ref for KRAS","Shoichi Matsukuma, Mitsuyo Yoshihara, Tetsuji Suda, Manabu Shiozawa, Makoto Akaike, Tomokazu Ishikawa, Shiro Koizume, Yuji Sakuma, Yohei Miyagi. Differential detection of KRAS mutations in codons 12 and 13 with a modified loop-hybrid (LH) mobility shift assay using an insert-type LH-generator. Clinica Chimica Acta 412, 1874-1878, 2011."]