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[" Target Gene Symbol/AS event","TAIR10 ID: AGI locus/gene model ","Forward primer 5' -  3'","Reverse primer 5' -  3'","PCR product size, base pairs","Application","RT-PCR program"]
["GOG","AT5G11980","ggtggtttttccacattgcg","ccttcgtatgcactcttggca",90,"RT-PCR/qRT-PCR","TD60x28 "]
["EF1a","At5G60390","tccagctaagggtgcc","ggtgggtactcggaga ",264,"RT-PCR/qRT-PCR","TD58x20 "]
["ACT2","AT3G18780","actttcatcagccgttttga","acgattggttgaatatcatcag","633 (gDNA), 199 (cDNA)","RT-PCR","TD58x30 "]
["LHY(I4S)*  ","AT1G01060","gaagaattattagctaaggcaagaaag","acgaggaggcggaatttcta",264,"RT-PCR/qRT-PCR","TD60x29"]
["LHY(I4R)**","AT1G01060.2","ggagaagaattattagctaaggcaag","aaaaagatctgaaaggatcatcaa",224,"RT-PCR/qRT-PCR","TD62x29"]
["LHY(I9S)*  ","AT1G01060.3","gatcggtgttggaacatgc","aacgagcgcctccaagtc",186,"RT-PCR/qRT-PCR","TD60x28  "]
["LHY(I9R)**","AT1G01060.3","aaccattaagcaatggatttgtc","caacgagcgccttcaagt",188,"RT-PCR","TD60x28  "]
["LCL1(I7S)*","AT5G02840"," aaatttcgaaactgttttgctg","aaccgtagaatgcatttgatagc",213,"RT-PCR/qRT-PCR","TD58x20 "]
["LCL1 (I7R)** ","AT5G02840","cctataaatttcgaaactgttttgct"," agggatgcggtataacacca",230,"RT-PCR/qRT-PCR","TD58x20 "]
["LCL1 (I8S)** ","AT5G02840.2","cctataaatttcgaaactgttttgct","aaccgtagaatgcatttgatagc",218,"RT-PCR","TD60x28  "]
["LCL1 (I8R)** ","AT5G02840.2","aactgttatattctcagcgttgc","ccgtagaatgcatctgacaca",135,"RT-PCR","TD60x29"]
["CCA1(I4S)*","AT2G46830","tcagaaatttttctccaaggtagag","ttcattggccatctcaggat",226,"RT-PCR/qRT-PCR","TD60x28 "]
["CCA1 (I4R)**","AT2G46830.1","cttgtgataagccatagaggtagag","tcattggccatctcagga",225,"RT-PCR/qRT-PCR","TD58x30 "]
["PRR7(I3S)*","AT5G02810 ","cggaacatccctgtcatcat","ttccactaccactagaactttgg",169,"RT-PCR","TD58x30 "]
["PRR7 (I3R)**  ","AT5G02810 ","gcgtatgttggaaagtagatgc","ttccactaccactagaactttgg",208,"RT-PCR","TD58x30 "]
["PRR9 (I3R)**","AT2G46790","ttttggttatggatcagttgc","tccatcagcttattgtgacca",172,"RT-PCR","TD58x30 "]
["GRP7(I1S)*","AT2G21660 ","tcgttctacacatctcactgctc ","tcgttaatgatcttggaatcaa",192,"RT-PCR","TD58x30 "]
["GRP7 (I1R)**   ","AT2G21660 ","ttccaaggtctgttacacgc","tcgttaatgatcgatttggg",184,"RT-PCR","TD58x30 "]
["GRP8 (I1R)**   ","AT4G39260","gcgacgttatcgattctaagg","cgttaatgatcgatgggaca",188,"RT-PCR","TD58x30 "]
["RVE2 (I1S)*  ","AT5G37260","tggctatgcaggaacgttgt","tcatgctctgcttctgtcca",133,"qRT-PCR ","TD60x29"]
["RVE2 (PCE)**    ","AT5G37260","ccatggctatgcaggttaaaa"," aaactctcacaacgttcctaatacg",131,"qRT-PCR ","TD60x29"]
["RVE2 (I3R)**","AT5G37260","tggagacgaatcgaaggtgt ","caaaatcgcgagcaacctta",225,"RT-PCR","TD60x29"]
["RVE7 (I1R)**  ","AT1G18330","gcagagttctgattgaggtttc","ttcctcacttcgatcctcag",213,"RT-PCR","TD60x29"]
["RVE8 (I7R)**  ","AT3G09600","ctccagtgcttcacggtgt","aggactttcctctgcagcat",390,"RT-PCR","TD60x29"]
["RVE8 (I8R)**  ","AT3G09600","ctccagtgcttcacggtgt","aaccatgaactcatgtgacttctaata",589,"RT-PCR","TD60x29"]
["CKB3 (I3R)** ","AT3G60250","aaagtttggtctgaaatcttgtatc","cacagttcttgtacttctcagtcattg",153,"RT-PCR","TD60x28  "]
["CKB3 (I4R)**","AT3G60250","aggaatggctgcaatgactg","tcaactgaagcaaaatcagacc",272,"RT-PCR","TD60x28  "]
["ASG4 (I6R)**","AT1G01520","ccaagttctgttatcaaagaggaa","tcatcagtaagagaacctgttgaa",345,"RT-PCR","TD60x28  "]
["* Primers were designed for RT PCR amplification of the fully spliced transcript lacking the specific AS event. ** The specific AS event is denoted in parentheses (IS - intron spliced, IR - intron retained)."]
["                                                                                      PCR amplification contitions"]
["Program","  PCR conditions"]
["TD60x28  ","950C - 20s, (950C - 15 sec., 650C- 20 sec., 720C - 60 sec.)x5 decreasing to 610C; (950C -15 sec., 600C - 20 sec., 720C - 60 sec.)x28; 720C 10 min."]
["TD58x30 ","950C -20sec., (950C - 15 sec., 650- 20 sec.,720- 60 sec.)x5 decreasing to 610C; (950C -15 sec., 580C - 20 sec., 720C - 60 sec.)x30; 720C - 10 min."]
["TD60x29","950C - 20 sec., (950C -15 sec., 650C -20 sec.,720C - 60 sec.)x5 decreasing to 610C; (950C -15 sec., 600C - 20 sec., 720C - 60 sec.)x29; 720C -10 min."]
["TD62x29","950C - 20s, (950C - 15 sec., 650C - 20 sec.,720C - 600 sec.)x4 decreasing to 620;(950C - 15 sec., 620C - 20 sec.,720C - 600 sec.)x29; 720C - 10 min."]
["TD58x20 ","950C - 20s, (950C - 15 sec., 650C -20 sec.,720C - 600 sec.)x5 decreasing to 610;(950C - 15 sec., 580C - 20 sec.,720C - 600 sec.)x20; 720C - 10 min."]
["CFX96 qRT PCR for all targets","950C-15 sec.; 940C- 15 sec., 560C - 30 sec. (at slow ramp rate 20C per sec.), 700C- 20C sec. (plate read), 750C-  15 sec. (plate read)] x 40 cycles; 650C to 950C in increments of 0.50C (melt curve, plate read).  "]
[]
[]
["  "]

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