3.2 Genbank pj5_00001.gb Genbank BMC_nterm_sig_pep_GFPuv.gb Genbank ccmN_nterm_sig_pep_GFPuv.gb Genbank ssrA_tag_GFPuv.gb Genbank ssrA_tag_enhanced_GFPuv.gb pNJH00010-vector_backbone origin_of_replication false 2016 1238 23d714d34632e24f53c2095f05e79f17aded7ec22651c20e4b320f82bc2bf731 forward 29.25 112.55 56 56 sig1 cds false 4 54 46d943915b196da710b25db3127c75a1ba01da913d0b5bb2b17055f9b5a079a1 forward 117.6 38.05 56 56 sig2 cds false 4 60 fbdc0cd5591545b773bce0741569577663dd940ca88bfd3b3dca98a3fc20fc39 forward 117.35 199.7 56 56 long cds false 55 102 46d943915b196da710b25db3127c75a1ba01da913d0b5bb2b17055f9b5a079a1 forward 215.25 40.35 56 56 short cds false 55 78 46d943915b196da710b25db3127c75a1ba01da913d0b5bb2b17055f9b5a079a1 forward Embed_in_primer_forward 217.25 205.05 56 56 gfpuv cds false 1242 1952 23d714d34632e24f53c2095f05e79f17aded7ec22651c20e4b320f82bc2bf731 forward 302.7 115.05 56 56 standard protein_stability_element false 1 15 de91216ea8484b510f9b055973f046ad3e8a11822971ca69e1029fd48f64c5b1 forward Embed_in_primer_reverse 391.3 42.05 56 56 enhanced protein_stability_element false 1 21 c3f5be2050eb4ff8857531a116a49c90984ddaf6a0493f649c6d43a3b5e11c65 forward Embed_in_primer_reverse 395.3 203.05 56 56 ssrA_tag_3prime protein_stability_element false 16 33 de91216ea8484b510f9b055973f046ad3e8a11822971ca69e1029fd48f64c5b1 forward Embed_in_primer_forward 483.65 123.05 56 56 rule1 1321377138753663 NOTMORETHAN 1 rule2 1321377138753663 NOTWITH 1321377138759456 rule3 1321377138753663 WITH 1321377138745859 pRDR00001-pRDR00008 vector_backbone false 1321377138742609 N-term_sig_pep true 1321377138745273 1321377138749459 Gly_Ser_linker false 1321377138751890 1321377138754401 gfpuv false 1321377138755522 ssrA_tag_5prime true 1321377138757577 1321377138759398 ssrA_tag_3prime false 1321377138760109 17 23 554 253