{"text_format":"3","workbook":"xls","n_sheets":5,"sheets":["sensory neurons","motor neurons","muscles","intestine","X box"],"not_rendered":{"external_links":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"sensory neurons","index":0,"n_rows":21,"n_cols":7,"rows_kept":21,"flags":["formulas_cached_only"]}
["Rank","spot number","cDNA name","CDS","locus","WormBase ID","Description"]
[38,1895,"yk456f10","K08C7.4","-","Expr448","Several pairs of nuclei around the terminal bulb of the pharynx show expression. These nuclei give rise to processes that terminate at the buccal cavity (visible only with GFP), and are therefore probably amphid sensory neurons. In younger larvae only one pair of neurons show expression."]
[57,3135,"yk425a9","Y102E9.1","odr-4","Expr1432","chemosensory neurons"]
[79,3328,"yk233e3","F38B2.1","ifa-1","Expr1495","The A1 promoter gfp reporter was expressed in cells associated with amphid sensory neurons, the pharyngeal-intestinal valve, the vulva, a few yet unidentified neurons, as well as in the rectum and some neurons of the tail. In addition, there was some mosaicism for small and as yet unidentified muscles of the pharynx. Not all A1 promoter gfp worms showed GFP expression in these cells."]
[157,1944,"yk486h11","F38G1.1","che-2","Expr1157","Most of the GFP produced from pCHE-2::GFP3 was localized at the cilia of most ciliated sensory neurons. This could be seen most clearly in amphid sensory neurons, whose cilia are fasciculated. The processes and cell bodies showed only faint fluorescence, and the nuclei were not fluorescent. The che-2::GFP transgenics pCHE-2::GFP1 and pCHE-2::GFP2 first expressed GFP in some head neurons between the comma and 1.5-fold stage of embryogenesis. The number of cells expressing GFP increased, as the development proceeded to the adult stage. The expression at the adult stage was detected in all the amphid sensory neurons except AFD, phasmid neurons PHA and PHB, all the inner and outer labial neurons (IL1, IL2, OLQ and OLL), CEP, PDE, FLP, PQR, and three unidentified neurons (perhaps AQR and ADEL/R). Thus, che-2 seems to be expressed in all the ciliated sensory neurons except BAG and AFD. GFP expression in N2 male tails, where there are many male-specific sensory neurons required for mating behavior. GFP was expressed at least in all the rays, which have ciliated sensory neurons."]
[361,9448,"yk539f11","C05D2.1","daf-4","Expr220","similar to daf-1. many head neurons incl amphid neurons"]
[452,5923,"yk599e7","F53A2.4","nud-1","Expr2419","In all adult and larval stages fluorescence was observed in the amphid and phasmid sensory neurons, as well as the neuronal processes extending to the nerve ring and preanal ganglion. Transient expression was also observed in the mid-ventral region of younger animals up to L2 stage corresponding to the location of the gonadal primordium. Weak fluorescence was also seen in intestinal and hypodermal regions of larval and adult stages and uv cells connecting the uterus and vulva. In addition, diffuse early embryonic expression was seen in all lines."]
[660,7971,"yk40a8","B0240.3","daf-11","Expr510","Expressed in a subset of amphid neurons (ASI, ASJ, ASK, AWB, and AWC cells."]
[675,1133,"yk354b8","F53H4.1","csb-1","Expr1917","Reporter_gene Assay: GFP first appeared during the 50^100 cell stage and was detected throughout the embryonic development. GFP expression was also observed in all of the somatic cells up to the L3 larval stage. However, GFP expression was relatively stronger in pi and P lineage vulval cells at the L3 larval stage, where active cell division and dierentiation take place. At the L4 larval stage, GFP was relatively stronger in amphid and tail neurons, and vulva and somatic gonad cells than in other body regions. In adult hermaphrodites, the overall GFP expression weakened, but strong expression was still observed in intestine, head, and tail regions. In L4 and adult males, the tail region, including the spicule, protractor, and PC sensilla, showed intense uorescence."]
[871,7322,"yk13d11","F18C12.1","che-3","Expr971","The highest and most consistent expression was observed with several independent strains derived from the largest expression construct (DYNGFP1B-7). Expression was nearly exclusive to neurons which possessed ciliated sensory specializations. The extent and level of expression correlated roughly with the growth of the ciliated endings of these neurons. Since neurulation (and presumably cilia growth) occurs in ovo, and since cells at this stage of development are compact and difficult to identify, the authors did not demonstrate that all ciliated neurons express the fusion construct. However, a subset of neurons with ciliated endings (ADL, ASH, ASI, ASJ, PHA, PHB, AQR, and PQR) generally show modest and somewhat variable expression of the fusion construct throughout the life span of the animal. Expression was first noted at approximately 380 - 400 min after fertilization-a time corresponding to the comma stage of development in two cells flanking the developing pharynx. Expression rapidly increased in extent and intensity, peaking in the 3-fold stage in many cells (>30) located anterior and posterior to the nerve ring, as well as in four cells in the developing tail. Expression in the region adjacent to the nerve ring was consistent with all ciliated neurons in this region showing fluorescence. Expression was maintained through most of embryogenesis but decreased in extent and intensity shortly after hatching; late L1 larvae typically showed expression in the phasmids (PHA and PHB) and five or six neuron classes in the head, which usually included ADL, ASK, ASH, ASJ, and ASI but also variably included a subset of ASE, ASG, ADF, AFD, FLP, and ADE. With occasional exceptions, most of the neuron classes anterior to the nerve ring, which based on position may have been BAG, IL1, IL2, OLL, CEP, and OLQ, ceased expressing the reporter late in embryonic development. The neurons with the most elaborate ciliated endings-the amphid wing cells, AWA, AWB, and especially AWC-did not show expression after hatching. Expression did not decrease much further than this as the animal aged. Both these cells showed expression in developing larvae from the L3 stage on. The postdeirid structure in the worm's midbody region also appears postembryonically and contains a ciliated neuron (PDE) that showed clear fluorescence in many animals. This expression was also transiently restricted to a period of time shortly following the appearance of this neuron. In addition, two examples of weak expression in unciliated cells were noted. First, at least one pharyngeal neuron (tentatively identified as I4) showed weak expression in some animals. Finally, barely perceptible fluorescence was occasionally observed in the coelomocytes of some worms."]
[955,8914,"yk94g1","F08F8.3","kap-1","Expr1272","High intensity of staining of the sensory cilia."]
[1404,4167,"yk465b12","F29C4.1","daf-1","Expr219","many head neurons including amphid chemosensory neurons"]
[1580,1590,"yk496e12","F55C10.1","cnb-1","Expr1826","The expression pattern of the predicted calcineurin B was similar to the pattern of tax-6::gfp in pAK43. See Expr1824: pAK43 drove TAX-6 expression in many sensory neurons, as well as interneurons including AIY and AIZ, and most, if not all, muscle cells."]
[1898,9259,"yk4d12","C02F4.2","tax-6","Expr1823","pAK13 drove TAX-6 expression mostly in sensory neurons, including the thermosensory AFD neurons, the chemosensory ASE, AWA, and AWC neurons, and the osmosensory ASH neurons."]
[2367,5268,"yk586f12","F10C1.2","ifb-1","Expr1497","The B1 promoter-driven GFP staining was seen in cells associated with the amphid sensory neurons, the excretory cells, the vulva, and the uterus and, finally, in the rectum and some neurons of the tail. In the pharynx, staining localized to the marginal cells and the pharyngeal-intestinal valve."]
[2640,3702,"yk310b9","B0240.3","daf-11","Expr510","Expressed in a subset of amphid neurons (ASI, ASJ, ASK, AWB, and AWC cells."]
[3926,8886,"yk611e1","Y41G9A.1","osm-5","Expr855","GFP expression in animals transgenic for pCJ3 was first observed around the 2-fold stage of embryogenesis and continued throughout development and in the adult worm. The pattern of GFP fluorescence was consistent with expression in all ciliated sensory neurons including the amphids, phasmids, and inner and outer labials. GFP expression was also observed in the sensory rays of the male tail."]
[4168,339,"yk94g1","F08F8.3","kap-1","Expr1272","High intensity of staining of the sensory cilia."]
[5461,9212,"yk607c4","F46A9.6","mec-8","Expr267","head neurons(incl amphid) tail neuron"]
[5688,764,"yk270b7","D2030.10","aex-1","Expr1805","pAX3: Expression of this fusion protein was observed in intestine, some amphid neurons, and body-wall muscles. Anterior and posterior, but not central, intestinal cells had detectable GFP fluorescence. Four neurons in the amphid had observable GFP fluorescence; this was observed only at a late adult stage when using pAX3 but not when using pAX4. The exact identities of these neurons are uncertain, but based on their positions, they do not appear to be the GABAergic AVL and DVB neurons required for the defecation behavior."]
[7050,5393,"yk501a6","F10C1.2","ifb-1","Expr1497","The B1 promoter-driven GFP staining was seen in cells associated with the amphid sensory neurons, the excretory cells, the vulva, and the uterus and, finally, in the rectum and some neurons of the tail. In the pharynx, staining localized to the marginal cells and the pharyngeal-intestinal valve."]

{"sheet":"motor neurons","index":1,"n_rows":10,"n_cols":7,"rows_kept":10,"flags":["formulas_cached_only"]}
["Rank","spot number","cDNA name","CDS","locus","WormBase ID","Description"]
[4641,1784,"yk359a6","ZC416.8","unc-17,cha-1","Expr320","head: IL2 URA URB SAA SAB SIA SIB SMB SMD RMD RIM, sev more; phar: M1 M2 M5 I1f I6f, others; body: VA VB VC DA DB AS SDQ HSNf; tail: ALN PLN others [J. Duerr (personal communication to Shawn Lockery)], antibody"]
[5264,720,"yk425a9","yk183c3","cho-1","Expr961","Expression of GFP detected in the nervous system, including the nerve ring and cholinergic motor neurons of the ventral nerve cord."]
[5284,1987,"yk115f12","C09B8.7","pak-1","Expr709","Expression first detected in embryos though to adulthood. In L1, strong staining detected in the pharynx and in 2 laterally located cells (NLS-lacZ staining). The pharyngeal cells are muscle cells which occupy most of the body of the pharynx and the lateral cells were CAN neurons based on the position of the nucleus and axon morphology. Both pharyngeal muscle and CAN neurons express reporter as embryo through to adulthood. Also stained were several cells in the tail region. These include the B and Y cells (forming male spicule) from L1 to adult, the hypodermal blast cells T in the L1 and some of its progeny in later stages. In L1, subset of cells in ventral nerve cord and DA identified as DB neurons (cholinergic motor neurons innervating dorsal body wall muscles) stain. Cells identified by GFP: GFP without NLS revealed expression in distal tip cells (DTC) spermatheca and uterus. It is not clear if GFP is expressed by somatic cells of the gonad or by the germline cells (namely sperm) in the spermatheca. Expression in uterus was seen outside the embryos. It is not clear which cells expressed the reporter in uterus although observation of NLS-lacZ and NLS-GFP indicated that some somatic cells of the gonad expressed CePAKGFP without NLS. There is expression in many hypodermal cells surrounding the embryos during the morphogenetic stage. C-terminus two amino-acids of CePAK replaced with GFP coding sequence and fusion protein was expressed from the CePAK promoter used to analyze intracellular localization (pCPK2.77). CePAK GFP was enriched at cell surface in pharyngeal muscle cells especially condensed at cell boundaries. In CAN neurons CePAK GFP detected in cell bodies and along the axons. CePAK GFP localized to cell bodies and their processes running along the excretory canal on the lateral surface of adults."]
[5292,9284,"yk236b1","Y16B4A.1","unc-3","Expr326","ASI DB DA VB VA AS. L1 stage = DA and DB motor neurons, ASI amphid neuron and other head cells. L2 stage = also VA VB and AS motor neurons, all 5 classes of excitatory neurons continue to express into adult hood."]
[6058,7986,"yk468c1","C09B8.7","pak-1","Expr709","Expression first detected in embryos though to adulthood. In L1, strong staining detected in the pharynx and in 2 laterally located cells (NLS-lacZ staining). The pharyngeal cells are muscle cells which occupy most of the body of the pharynx and the lateral cells were CAN neurons based on the position of the nucleus and axon morphology. Both pharyngeal muscle and CAN neurons express reporter as embryo through to adulthood. Also stained were several cells in the tail region. These include the B and Y cells (forming male spicule) from L1 to adult, the hypodermal blast cells T in the L1 and some of its progeny in later stages. In L1, subset of cells in ventral nerve cord and DA identified as DB neurons (cholinergic motor neurons innervating dorsal body wall muscles) stain. Cells identified by GFP: GFP without NLS revealed expression in distal tip cells (DTC) spermatheca and uterus. It is not clear if GFP is expressed by somatic cells of the gonad or by the germline cells (namely sperm) in the spermatheca. Expression in uterus was seen outside the embryos. It is not clear which cells expressed the reporter in uterus although observation of NLS-lacZ and NLS-GFP indicated that some somatic cells of the gonad expressed CePAKGFP without NLS. There is expression in many hypodermal cells surrounding the embryos during the morphogenetic stage. C-terminus two amino-acids of CePAK replaced with GFP coding sequence and fusion protein was expressed from the CePAK promoter used to analyze intracellular localization (pCPK2.77). CePAK GFP was enriched at cell surface in pharyngeal muscle cells especially condensed at cell boundaries. In CAN neurons CePAK GFP detected in cell bodies and along the axons. CePAK GFP localized to cell bodies and their processes running along the excretory canal on the lateral surface of adults."]
[6300,2574,"yk492g9","F41C6.1","unc-6","Expr1590","Early Expression by Ventral Epidermoblasts: Ventral epidermoblasts P1/2-P11/12 are the first cells to express UNC-6::HA during embryogenesis. Netrin becomes detectable in the cytoplasm of these twelve cells soon after their births (circa 230 min) and before they form into regular rows. This staining gradually decreases in intensity as the cells spread ventrally, forming two symmetrical rows. At the completion of neurulation, soon after these rows have come together along the ventral midline, cytoplasmic staining is no longer detectable.\nExpression by Larval Ventral Cord Motorneurons: Postembryonic motorneurons VA2VA12 and VB3VB11 express UNC-6::HA in the ventral nerve cord. Netrin becomes detectable in these cells soon after their births at the end of the first larval stage. This expression continues through the adult stage. After hatching, the C. elegans larva increases about 5-fold in both length and circumference before becoming an adult. Six axons (AVAL, AVAR, AVBL, AVBR, AVG, and PVQR) in the right longitudinal tract and one axon (PVQL) in the left tract provide a continuous source of UNC-6 in the ventral nerve cord. Near the end of the first larval stage, the twenty postembryonic motorneurons (VA2VA12, VB3VB11) provide a further source of UNC-6 in the right tract. These motorneurons could maintain and sharpen the proposed gradient of netrin in the basement membrane of the epidermis and skew its peak farther to the right of the ventral midline.\nExpression by Lateral Ring and Lumbar Ganglia Neurons: Paired neurons AVA, AVB, and PVQ express UNC-6::HA in the lateral and lumbar ganglia, respectively. Born circa 300 min, netrin does not become detectable in these cells until about 3-fold elongation. Netrin expression then continues through the larval and adult stages. After axogenesis begins, PVQ itself expresses UNC-6.\nExpression by a Midline Neuron in the Developing Pharynx: The midline neuron I5 expresses UNC-6::HA in the developing pharynx. Netrin becomes detectable in this cell soon after its birth (circa 280 min). Positioned on the ventral midline, I5 is the most posterior neuron in the pharynx at this time. Netrin expression is transient; UNC-6::HA is no longer detectable in the cell body after about 3-fold elongation, but staining in the axons persists somewhat longer.\nExpression by Midline and Asymmetric Neurons in the Developing Nerve Cord: Positioned at the anterior and posterior ends of the developing ventral nerve cord, respectively, the midline neurons AVG and PVT express UNC-6::HA. Netrin becomes detectable in the PVT neuron soon after its birth (circa 290 min). At this time, PVT is positioned on the ventral midline of the body wall just anterior to the developing rectum. Netrin expression is transient; UNC-6::HA is no longer detectable in this cell by 3-fold elongation. By then, PVT has shifted to its mature position as the most anterior cell in the preanal ganglion. Born circa 290 min, AVG does not express UNC-6::HA until about 3-fold elongation. AVG is positioned on the ventral midline in the retrovesicular ganglion at this time. Netrin expression then continues through the larval and adult stages. Besides AVG, a second neuron in the retrovesicular ganglion, RIFL (born circa 410 min), also expresses UNC-6::HA from about 3-fold elongation through adult. Interestingly, no UNC-6::HA expression is ever observed in the bilateral homolog RIFR.\nExpression by Sheaths in the Developing Head: All six inner labial and both ventral cephalic sheaths express UNC-6::HA in the early neurula. Netrin becomes detectable in these cells soon after their births (circa 310 min). At this time, the inner labial sheaths are clustered together just anterior to the developing pharynx. As the head depression forms around comma stage, the cell bodies move anteriorly toward the sensillar rudiments, revealing processes trailing back to the developing nerve ring. The endfeet form a more-or-less complete path around the pharynx that anticipates the anterior margin of the nerve ring. UNC-6::HA is detectable along the entire cell body and process at this time. As the embryo elongates, the cell bodies initially stay near the sensillar rudiments at the tip of the head. Presently, as judged by UNC-6::HA staining, the processes no longer reach the developing nerve ring. By inference, they fail to stretch apace with the rapid elongation of the head. As the pharynx elongates, the cell bodies assume their mature positions, typically just anterior or posterior to the metacorpus. Netrin expression is transient; only weak staining of UNC-6::HA is detectable in these cells beyond 3-fold elongation. The sheath processes appear to provide a scaffold of netrin-labeled pathways that support and guide labial axons to the nerve ring. Moreover, their endfeet could form a ring-shaped substratum for axons within the ring itself. The cephalic sheaths are positioned dorsally and ventrally just anterior to the developing nerve ring. In the early neurula, these cells extend sheetlike processes that apparently anticipate the outer surface of the ring neuropil. At this time, UNC-6::HA is detectable throughout the cell body and processes of the ventral cephalic sheaths. Netrin expression is transient; UNC-6::HA is no longer detectable in these cells by 3-fold elongation. Finally, no UNC-6::HA expression is ever observed in the dorsal cephalic sheaths."]
[7132,9081,"yk523d12","Y16B4A.1","unc-3","Expr326","ASI DB DA VB VA AS. L1 stage = DA and DB motor neurons, ASI amphid neuron and other head cells. L2 stage = also VA VB and AS motor neurons, all 5 classes of excitatory neurons continue to express into adult hood."]
[7418,440,"yk278g9","ZC416.8","cha-1","Expr217","head: IL2 URA URB SAA SAB SIA SIB SMB SMD RMD RIM, sev more; phar: M1 M2 M5 I1f I6f, others; body: VA VB VC DA DB AS SDQ HSNf; tail: ALN PLN others [jduerr(personal communication to Shawn Lockery) , antibody]"]
[7998,9427,"yk512f6","C09B8.7","pak-1","Expr709","Expression first detected in embryos though to adulthood. In L1, strong staining detected in the pharynx and in 2 laterally located cells (NLS-lacZ staining). The pharyngeal cells are muscle cells which occupy most of the body of the pharynx and the lateral cells were CAN neurons based on the position of the nucleus and axon morphology. Both pharyngeal muscle and CAN neurons express reporter as embryo through to adulthood. Also stained were several cells in the tail region. These include the B and Y cells (forming male spicule) from L1 to adult, the hypodermal blast cells T in the L1 and some of its progeny in later stages. In L1, subset of cells in ventral nerve cord and DA identified as DB neurons (cholinergic motor neurons innervating dorsal body wall muscles) stain. Cells identified by GFP: GFP without NLS revealed expression in distal tip cells (DTC) spermatheca and uterus. It is not clear if GFP is expressed by somatic cells of the gonad or by the germline cells (namely sperm) in the spermatheca. Expression in uterus was seen outside the embryos. It is not clear which cells expressed the reporter in uterus although observation of NLS-lacZ and NLS-GFP indicated that some somatic cells of the gonad expressed CePAKGFP without NLS. There is expression in many hypodermal cells surrounding the embryos during the morphogenetic stage. C-terminus two amino-acids of CePAK replaced with GFP coding sequence and fusion protein was expressed from the CePAK promoter used to analyze intracellular localization (pCPK2.77). CePAK GFP was enriched at cell surface in pharyngeal muscle cells especially condensed at cell boundaries. In CAN neurons CePAK GFP detected in cell bodies and along the axons. CePAK GFP localized to cell bodies and their processes running along the excretory canal on the lateral surface of adults."]

{"sheet":"muscles","index":2,"n_rows":54,"n_cols":7,"rows_kept":54,"flags":["formulas_cached_only"]}
["rank","spot number","cDNA name","CDS","locus","WormBase ID","Description"]
[106,8552,"yk608c8","C44B12.2","ost-1","Expr707","Expression is first detected in unhatched larvae and was maintained throughout all larval stages and in adults. Staining was observed along the length of the nematode and was particularly strong in cells along the body wall where beta-gal also accumulates in a striated pattern in the cytoplasm. The striated pattern is identified as body wall muscle cells. In addition, sex muscle cells show staining. There are two clusters of nuclei visible on both sides of the vulva. Staining is observed around the pharynx but there is no staining observed in pharyngeal muscle."]
[188,6582,"yk596g1","F02A9.3","far-2","Expr1","Body wall muscle cells and vulval muscle cells of adult hermaphrodites. Beta-galactosidase is nuclear localized"]
[195,8183,"yk589h1","Y105E8B.1","lev-11","Expr723","PTMIZ2328: this fusion gene was specifically expressed in the body wall muscles, the anal muscles, the vulva muscles and the male sex muscles except the pharyngeal muscles. pTMIIIZ3256: this fusion gene expressed only in the pharyngeal muscles."]
[275,2093,"yk327f9","C44B12.2","ost-1","Expr707","Expression is first detected in unhatched larvae and was maintained throughout all larval stages and in adults. Staining was observed along the length of the nematode and was particularly strong in cells along the body wall where beta-gal also accumulates in a striated pattern in the cytoplasm. The striated pattern is identified as body wall muscle cells. In addition, sex muscle cells show staining. There are two clusters of nuclei visible on both sides of the vulva. Staining is observed around the pharynx but there is no staining observed in pharyngeal muscle."]
[287,742,"yk274f2","F01G12.5","let-2","Expr519","Body wall muscle at embryo. Presumptive GLRDL, GLRDR, GLRL, GLRR, GLRVL, GLRVR, coelomocytes, and anal muscles show expression in addition to body muscle cells at L1, L2, L3 L4 and adult. Distal tip cells of developing gonad in L2, L3, and L4. Spermathecal cells and distal ends of gonad show expression at L4 and adult. Vulval and uterine muscles in adult."]
[393,5302,"yk597f2","F54C1.7","pat-10","Expr1194","Staining in the body wall muscles, the vulva muscles, and the minor muscles of the anal region."]
[491,1952,"yk54d5","ZK973.6","anc-1","Expr2220","Expressed in body wall muscles."]
[581,5687,"yk556f1","F15G9.4","him-4","Expr879","Excepting body wall muscles and gonadal leaders, described below, no other cells were observed to express SP-GFP-hemicentin. Body wall muscles: Beginning in late embryogenesis (approx. 500 minutes), and continuing through adulthood, hemicentin is synthesized by lateral body wall muscle cells along the entire length of the animal, excepting cells six and eight near the nerve ring. Medial body wall muscle cells express little if any hemicentin. Gonadal leaders (hermaphrodite): Beginning soon after their genesis in late L1 stage, hermaphrodite distal-tip cells express hemicentin continuously throughout development. The anchor cell, or proximal leader, expresses hemicentin beginning in early L3 stage soon after cell commitment. SP-GFP-hemicentin diffuses throughout the utse syncytium at this time, but it is not known whether him-4 is transcribed in nuclei other than the anchor. In the adult, all expression within the utse syncytium ceases. Gonadal leaders (male): In the male, distal-tip cells never express hemicentin. The linker cell, or proximal leader, expresses hemicentin beginning at early L2 stage."]
[790,3285,"yk124f5","M03F4.2","act-4","Expr590","act-4::beta-gal fusion protein was detected from embryonic stages through adult developmental stages. In adult hermaphrodites from strain EH19 containing extrachromosomal array of pJS303, strong staining was observed in body wall muscles, vulva muscle and spermatheca(fixed animals were stained with either Xgal or with anti-beta-gal antibody). pSS22 beta-gal was detected in body wall muscle. Lambda act-16-lacZ staining observed in vulval muscle, spermatheca and pharyngeal cells (possibly pharyngeal muscle)."]
[884,3840,"yk478e8","F40E10.3","csq-1","Expr1279","Different levels of GFP were observed in the body-wall muscle depending on the fusion constructs."]
[980,5664,"yk628d8","C18A11.7","dim-1","Expr2452","Expressed throughout development from the 1.5-fold stage of embryogenesis and appears to be limited to bodywall muscles. Anti-GFP immunofluorescence was not observed in any of the other muscle types."]
[993,5303,"yk597e7","C18A11.7","dim-1","Expr2452","Expressed throughout development from the 1.5-fold stage of embryogenesis and appears to be limited to bodywall muscles. Anti-GFP immunofluorescence was not observed in any of the other muscle types."]
[1078,6659,"yk80g8","F01G12.5","let-2","Expr519","Body wall muscle at embryo. Presumptive GLRDL, GLRDR, GLRL, GLRR, GLRVL, GLRVR, coelomocytes, and anal muscles show expression in addition to body muscle cells at L1, L2, L3 L4 and adult. Distal tip cells of developing gonad in L2, L3, and L4. Spermathecal cells and distal ends of gonad show expression at L4 and adult. Vulval and uterine muscles in adult."]
[1347,3304,"yk177a1","T18D3.4","myo-2","Expr1537","Strong activity in pharynx muscle."]
[1530,8163,"yk570c7","F55A8.2","egl-4","Expr2383","GFP was expressed in body wall muscles."]
[1840,9416,"yk540g7","R12E2.7","-","Expr2026","Expression is seen from 3-fold embryo stage, through all larval stages and through to adulthood. Punctate cytoplasmic and nuclear expression is quite extensive and is seen throughout the worm in bodywall muscle and hypodermis. More specifically the vulC cells of the vulva frequently show strong expression, as do cells of the defecation muscle system."]
[1910,3415,"yk337c4","F01G12.5","let-2","Expr519","Body wall muscle at embryo. Presumptive GLRDL, GLRDR, GLRL, GLRR, GLRVL, GLRVR, coelomocytes, and anal muscles show expression in addition to body muscle cells at L1, L2, L3 L4 and adult. Distal tip cells of developing gonad in L2, L3, and L4. Spermathecal cells and distal ends of gonad show expression at L4 and adult. Vulval and uterine muscles in adult."]
[2451,8574,"yk336c8","F18G5.3","gpa-12","Expr2374","Expressed throughout development in pharyngeal muscle cells that control pumping of the pharynx and in hypodermal cells."]
[2523,2464,"yk357a11","F15G9.4","him-4","Expr879","Excepting body wall muscles and gonadal leaders, described below, no other cells were observed to express SP-GFP-hemicentin. Body wall muscles: Beginning in late embryogenesis (approx. 500 minutes), and continuing through adulthood, hemicentin is synthesized by lateral body wall muscle cells along the entire length of the animal, excepting cells six and eight near the nerve ring. Medial body wall muscle cells express little if any hemicentin. Gonadal leaders (hermaphrodite): Beginning soon after their genesis in late L1 stage, hermaphrodite distal-tip cells express hemicentin continuously throughout development. The anchor cell, or proximal leader, expresses hemicentin beginning in early L3 stage soon after cell commitment. SP-GFP-hemicentin diffuses throughout the utse syncytium at this time, but it is not known whether him-4 is transcribed in nuclei other than the anchor. In the adult, all expression within the utse syncytium ceases. Gonadal leaders (male): In the male, distal-tip cells never express hemicentin. The linker cell, or proximal leader, expresses hemicentin beginning at early L2 stage."]
[2671,1648,"yk111f9","F49B2.5","src-2","Expr2314","Expressed in pharyngeal muscles, the vulva and some cells around anus."]
[2725,4787,"yk411b8","C24A3.6","twk-18","Expr2447","Expressed in body-wall muscle."]
[3021,6652,"yk25c5","F07C3.4","-","Expr172","Expression is seen in all bodywall and vulval muscle cells from early larval to adult stage. In the bodywall muscle expression is localised to discrete subcellular organelles that are distributed throughout the cell, but are not associated with the thick filaments."]
[3036,796,"yk340d11","Y71G12B.14","lsm-6","Expr2638","GFP is seen throughout the body wall and sex muscle cells. Confocal microscopy of dissected gonad arms shows GFP expression in the DTC and in the gonad sheath cells."]
[3085,1251,"yk481d9","F46B6.3","smg-4","Expr1337","Pers Comm. from Rachel Aronoff 11-29-01: clear expression in adult muscle and in embryonic cells of many lineages, including myocytes."]
[3515,2035,"yk264e5","T21C12.1","unc-49","Expr1283","Fluorescence was detected mainly on head muscles and body wall muscles on both the dorsal and ventral sides. Strong spincter muscle GFP was observed in unc-49B::gfp but not unc-49C::gfp animals. Both constructs produce variable, weak fluorescence in head ganglia."]
[3644,6678,"yk12b8","F41C3.2","-","Expr96","The expression pattern appears to have two components, both in muscle cells, and for both the b-galactosidase appears in discrete sub-cellular foci.The first component begins in late embryogenesis and continues through early larval stages. Four longitudinal bands of b-galactosidase staining are observed running from just behind the pharynx to the posterior of the worms. This may correspond to to the body wall muscle cells, apart from those body wall muscle cells in the head.The second component begins in late larval stages as the gonad develops. b-galactosidase staining is first diffuse, in four patches a-p along the gonad. It then becomes more discrete as the subcellular foci form although apparently still in four bands, which we think correspond to the uterine muscle cells. As the adult hermaphrodites age, these foci seem to become progressively more specifically associated with lateral positions and we hypothesise that these foci are related to the attachments of the uterine muscle cells to the hypodermal seam cells. Expression in males has not yet been examined."]
[3688,4496,"yk459a3","C52E4.2","mif-2","Expr1733","The cellular expression pattern of the fusion protein suggested that Ce-MIF-2 was produced in the body wall muscles and the muscle of the vulva. In addition, strong Ce-MIF-2-gfp activity was associated with the hypodermis."]
[3760,5261,"yk585e9","F15D3.1","dys-1","Expr1265","dys-1::gfp VIII was expressed in the body wall muscles, the vulva muscles, the head muscles and the pharyngeal muscles. Staining was observed from 3-fold embryo to adulthood. No staining was detected in non-muscle cells. Other constructs containing shorter 3' sequences gave no or very weak signal."]
[3874,4445,"yk410e3","F11C3.2","unc-122","Expr2860","Expression of UNC-122 was observed in body wall muscle and coelomocytes."]
[3898,8635,"yk534g7","C08B11.5","sap-49","Expr1259","Expression observed only in specific cells during most developmental stages after hatching. The cell identity cannot be fully determined. Some were identified as the pharyngeal muscle cells (m3-m8)"]
[4044,9079,"yk523e12","B0304.1","hlh-1","Expr1389","The pattern of expression in larvae and adults was identical whether assayed by antibody staining or reporter gene. CeMyoD is expressed only in body wall muscle cells. CeMyoD antibody stained in the nuclei of very early embryonic blastomeres at about 120 min postfertilization. Staining initially is seen only in the two daughter cells of the D blastomere. Shortly after, CeMyoD is observed in the two daughters of both C.ap and C.pp. Staining is not seen in any of the embryonic blastomeres that produce other types of tissues, including pharyngeal muscle."]
[4171,5930,"yk600e8","K08E3.8","mdt-29","Expr2134","Expression is confined to adults, and consists of faint non-nuclear punctate expression in bodywall muscle cells."]
[4337,8483,"yk562d11","C08B11.5","sap-49","Expr1259","Expression observed only in specific cells during most developmental stages after hatching. The cell identity cannot be fully determined. Some were identified as the pharyngeal muscle cells (m3-m8)"]
[4390,7532,"yk575d2","W05F2.4","-","Expr2106","Strong nuclear and cytoplasmic expression is seen in all bodywall muscle and vulval muscle cells. Expression is most often seen in elongating embryos and young larvae, but is also seen in older larvae and adults."]
[5121,4976,"yk250a12","F33D4.2","itr-1","Expr826","Expression of pA is observed in the vulva, rectal epithelial (Rep) cells and anterior muscle cells of the pharyngeal terminal bulb."]
[5217,1782,"yk322h8","F15G9.4","him-4","Expr879","Excepting body wall muscles and gonadal leaders, described below, no other cells were observed to express SP-GFP-hemicentin. Body wall muscles: Beginning in late embryogenesis (approx. 500 minutes), and continuing through adulthood, hemicentin is synthesized by lateral body wall muscle cells along the entire length of the animal, excepting cells six and eight near the nerve ring. Medial body wall muscle cells express little if any hemicentin. Gonadal leaders (hermaphrodite): Beginning soon after their genesis in late L1 stage, hermaphrodite distal-tip cells express hemicentin continuously throughout development. The anchor cell, or proximal leader, expresses hemicentin beginning in early L3 stage soon after cell commitment. SP-GFP-hemicentin diffuses throughout the utse syncytium at this time, but it is not known whether him-4 is transcribed in nuclei other than the anchor. In the adult, all expression within the utse syncytium ceases. Gonadal leaders (male): In the male, distal-tip cells never express hemicentin. The linker cell, or proximal leader, expresses hemicentin beginning at early L2 stage."]
[5435,4118,"yk417c3","F44B9.2","-","Expr123","This simple expression pattern expresses in the body wall muscle and in the vm1 vulval muscles. The staining of the bodywall muscles is strongest in the head and anterior of the worm. Expression begins in 3-fold embryos, and is seen throughout larval stages to adulthood. The vulval muscles express in L4 larvae and adults. Both components display mosaic expression."]
[5587,7564,"yk605f8","F54C9.1","iff-2","Expr128","Expression is seen from embryo to adult in this extensive multicomponent pattern. At the 1.5-fold stage staining is seen in 5 as yet unidentified nuclei, with staining becoming more extensive in the 3-fold embryo. In larvae and adults, staining is seen in the pharynx (including pharyngeal muscle cell nuclei), with many hypodermal nuclei in the head, tail and body also staining. Expression is also seen in the excretory cell nucleus and canals, in the spermathecae, and in the anal muscles."]
[5601,8866,"yk600g1","Y40B10A.9","-","Expr2084","Occasional mosaic expression is seen older worms in bodywall muscle cells."]
[5620,283,"yk476h1","F52H3.1","let-268","Expr1065","Expression of let-268::GFP begins at the 1.5-fold stage and continues throughout development. let-268::GFP is detected in the body wall muscle cells and in the glial like cells (GLR cells). However, let-268::GFP was not observed in the distal tip cells of the gonad, the spermatheca, or the vulva muscles."]
[5669,3262,"yk129d6","F02A9.4","-","Expr62","Expression is evident in both the metacorpus and terminal bulb of the adult pharynx. B-gal staining seems to be sub-cellularly localised in the pseudocircular m4 muscle cells in the metacorpus; staining in the terminal bulb is restricted to the posterior of the bulb, and so may mark the location of the m7 muscles."]
[5741,3372,"yk303a12","F08C6.7","unc-98","Expr2577","Expression was first observed in the developing embryo at the 1.5- to 2-fold stage, also observed in larvae and adults. Expression observed in body wall muscle, anal depressor muscle and vulval muscles."]
[5766,3648,"yk240h2","T01H3.3","-","Expr2128","Diffuse expression is seen in predominantly older larvae and adults in a number of components, i.e. throughout the musculature of the pharynx, in bodywall muscle and vulval muscle cells, in processes composing the nerve ring, and in somatic cells of the spermathecae. This gene has homology to Na-dependent inorganic phosphate cotransporters."]
[5989,1121,"yk348f12","F58A3.2","egl-15","Expr1802","A stably integrated egl-15::gfp transgene is expressed in the four Type 1 vulval muscles (vm1s): one in each of the four egg-laying muscle quadrants."]
[6132,7608,"yk663f2","C47E8.7","unc-112","Expr944","GFP is found in the body wall, vulval, spermathecal, uterine, and anal sphincter/depressor muscles. A few unidentified cells also exhibit GFP expression, suggesting that UNC-112 may not be limited to contractile tissues."]
[6357,9934,"yk225d5","F33D4.2","itr-1","Expr826","Expression of pA is observed in the vulva, rectal epithelial (Rep) cells and anterior muscle cells of the pharyngeal terminal bulb."]
[6688,7942,"yk44f2","F30H5.1","unc-45","Expr1270","The reporter genes are detectable in the adult in the body wall muscle cells, in the pharyngeal muscle cells, in the sex-specific muscle cells (e.g., vulval muscles in hermaphrodites and diagonal muscles of the male tail) and in the anal muscles. Expression is also found in the embryos at the comma to two-fold stage, when muscle lattices are being assembled. beta-galactosidase staining from the lacZ fusion is detected in a small number of cells slightly earlier in development but the identity of these cells has not yet been determined. Expression is not seen in the gonads."]
[6733,2752,"yk343g1","T08G11.5","unc-29","Expr1465","Expressed in body muscle."]
[6835,1883,"yk430g9","C25A8.4","-","Expr420","Weak diffuse expression is seen only in the somatic gonad of adult worms. In particular, expression is seen in the uterine wall, in the vulval hypodermis, and in vulval muscle."]
[7435,8190,"yk590d7","ZC410.4","twk-8","Expr2446","Expressed in body-wall muscle."]
[7488,4931,"yk151f6","F57B7.4","mig-17","Expr1030","GFP fluorescence was observed on the pseudocoelomic face of body wall muscles, but not on their hypodermal face. This expression pattern was first detected in late embryos and continued through the adult stage. Expression of mig-17 was also seen on the surface of gonad, starting when the DTCs migrated over the lateral hypodermis toward the dorsal muscles."]
[7579,1558,"yk437e2","F33D4.2","itr-1","Expr826","Expression of pA is observed in the vulva, rectal epithelial (Rep) cells and anterior muscle cells of the pharyngeal terminal bulb."]
[8269,2284,"yk36b3","ZK973.6","anc-1","Expr2220","Expressed in body wall muscles."]

{"sheet":"intestine","index":3,"n_rows":65,"n_cols":7,"rows_kept":65,"flags":["formulas_cached_only"]}
["rank","spot number","cDNA name","CDS","locus","WormBase ID","Description"]
[379,1925,"yk493c11","F46A9.4","skr-2","Expr2438","Expression of SKR-2::GFP was restricted to the intestine of transgenic animals."]
[594,9088,"yk524d4","F42G8.3","pmk-2","Expr1846","expressed throughout the intestine but was not localized to the nuclei under any tested conditions."]
[2742,1899,"yk458c1","Y17G7B.5","mcm-2","Expr2107","Expression is seen in all stages from early embryo, but is mosaic and sporadic. Expression is seen in the intestine, uterus, excretory cell and other cells."]
[2768,1012,"yk203b8","T05B4.2","nhr-57","Expr1181","CEP sheath cells; excretory gland cell; pharynx; intestine."]
[2997,1584,"yk468d5","C25A11.4","ajm-1","Expr1682","AJM-1 localizes to the apical borders of all C. elegans epithelia. Expression occurs in the embryonic hypodermis, pharynx and intestine, as well as in post-embryonic epithelia, including the vulva, uterus, spermathecae, pharynx, intestine, hindgut, hypodermis and male tail. MH27 staining of embryos expressing HMP-1GFP reveals that AJM-1 occupies an apical domain that is basal to the HMRHMP(cadherincatenin) complex in all epithelial tissues examined. Transmission electron microscopy (TEM) analysis of embryonic hypodermal cell junctions revealed an apical junctional domain resembling adherens junctions of flies and vertebrates, whereas no morphologically distinct septate or tight junctions are observed. The apicobasal extent of this domain is ~100 nm. Immuno-TEM analysis of hypodermal cells of larvae with the MH27 antibody revealed that AJM-1 specifically localizes to the apical domain."]
[3004,6667,"yk7c3","Y116A8C.33","-","Expr174","Weak nuclear expression is observed in the posterior intestinal cells of larval and adult stage worms. Occasionally, more extensive expression throughout the intestine is seen."]
[3153,3616,"yk134g10","B0261.2","let-363","Expr2227","Zygotic expression starts in the comma-stage embryos. From the early embryo stage to adulthood, CeTOR::GFP is expressed in all major tissues and organs and most, if not all, cells. Importantly, CeTOR is expressed in all the tissues and cells, such as the intestine, the gonad, and the hypodermal cells. These results are confirmed with several other stable lines containing extrachromosomal arrays from two additional translational fusion constructs."]
[3251,5634,"yk601g8","D1009.2","cyp-8","Expr744","cyp-8 protein as expression of beta-galactosidase was localized specifically in the gut cell nuclei. Expression is detected at all stages from embryo through to adult. Promoter region of Ce-CYP-8 examined with downstream coding sequence either including or excluding the NLS containing tail domain of cyp-8. This shows that non-cyclophilin tail domain directs the localization of this enzyme to the gut cell nuclei. Constructs containing promoter cyclophilin domain and tail domain exhibited nuclear localized gut staining in a non-nuclear localized vector. Animals transformed with the promoter and cyclophilin domain but lacking the NLS-rich tail domain show diffuse gut staining extending to the pharynx."]
[3280,1892,"yk457a3","R12A1.4","ges-1","Expr684","At 8 E cell stage, staining seen in developing intestine through to morphogenesis. In hatched L1 larvae, beta-gal detected only weakly and sporadically in the intestine."]
[3502,7459,"yk519g2","C25A11.4","ajm-1","Expr1682","AJM-1 localizes to the apical borders of all C. elegans epithelia. Expression occurs in the embryonic hypodermis, pharynx and intestine, as well as in post-embryonic epithelia, including the vulva, uterus, spermathecae, pharynx, intestine, hindgut, hypodermis and male tail. MH27 staining of embryos expressing HMP-1GFP reveals that AJM-1 occupies an apical domain that is basal to the HMRHMP(cadherincatenin) complex in all epithelial tissues examined. Transmission electron microscopy (TEM) analysis of embryonic hypodermal cell junctions revealed an apical junctional domain resembling adherens junctions of flies and vertebrates, whereas no morphologically distinct septate or tight junctions are observed. The apicobasal extent of this domain is ~100 nm. Immuno-TEM analysis of hypodermal cells of larvae with the MH27 antibody revealed that AJM-1 specifically localizes to the apical domain."]
[3600,8499,"yk585c2","F42G9.2","cyp-6","Expr1991","CYP-6 was expressed in the nematode gut. Expression was most abundant in the embryo, having a very early expression pattern, and was not expressed in later larval stages and adults."]
[3923,6233,"yk589d8","F28F8.5","-","Expr2008","Nuclear-localised expression is seen from late embryonic stages until adulthood in many, or all, cells of the intestine, somatic gonad, and hypodermis."]
[4011,2201,"yk470d6","F38A6.1","pha-4","Expr509","In the gut at the 8E cell stage; E descendants continue to show expression through late embryo. Pharyngeal cells derived from both AB and MS lineages show staining starting at comma stage. Intestinal and pharyngeal staining decline after hatching. The developing somatic gonad shows expression at L3 and L4. In comma to 1.5-fold stage embryos, hybridization was also seen in the tail region."]
[4346,7618,"yk13h11","H06O01.1","pdi-3","Expr2298","Prominent beta-galactosidase reporter expression was observed in many tissues, included the lateral seam cell hypodermis, and additionally strong staining was also observed in the gut cells."]
[4393,5511,"yk536d12","C11G6.4","nhr-28","Expr1180","Pharynx; intestine; hypodermis"]
[4406,8196,"yk602b4","F57C9.4","-","Expr2078","Mosaic speckly expression is seen from early embryos through to adults, in many cells, including intestine, hypodermis and somatic gonad."]
[4695,7508,"yk555e11","C10H11.1","-","Expr418","Expression is confined to 3-fold embryos and young larvae, and consists of very weak diffuse staining located within the intestine and hypodermis."]
[4815,901,"yk478e11","F31F6.6","nac-1","Expr2367","GFP expression is restricted to the intestinal tract, indicating that cenadc1 gene is expressed in the intestinal tract. The expression pattern is evident from the early larva stage through the adult stage. The GFP fluorescence is detectable throughout the intestinal tract, starting from the pharynx all the way through the anus. The expression level of GFP is significantly greater in the anterior half of the intestine than in the posterior half."]
[4960,5960,"yk608e7","W01G7.4","-","Expr408","Expression begins in 3-fold embryos and comprises a number of components. The strongest component is intestinal expression, especially in cells of the posterior intestine. Weaker, and highly mosaic expression, is seen in hypodermal cells and cells of the pharynx."]
[5075,425,"yk285g12","Y47G6A.23","lpd-3","Expr2349","lpd-3::GFP transgenic worms, like lpd-1 (SREBP)::GFP worms, displayed prominent GFP expression in the intestine."]
[5118,8534,"yk610b10","F31C3.1","cyp-5","Expr1990","The spatial expression pattern was examined using two complementary approaches, lacZ reporter transgene analysis and the more sensitive immunolocalisation studies with specific antiserum that definitively measures endogenous protein. Taken together these studies confirmed that CYP-5 is expressed exclusively in the nematode gut, from the embryonic to adult stages."]
[5121,4976,"yk250a12","F33D4.2","itr-1","Expr828","Expression of pC is observed in the intestine, pharyngeal isthmus and spermathecal (Sp) valve, uterine sheath cells and spermatheca."]
[5202,6289,"yk612a8","H06O01.1","pdi-3","Expr2298","Prominent beta-galactosidase reporter expression was observed in many tissues, included the lateral seam cell hypodermis, and additionally strong staining was also observed in the gut cells."]
[5306,6260,"yk588g4","B0280.1","-","Expr90","This pattern shows expression in the large nuclei of the intestine. Expression can be seen in late larval stages and in adults. Staining most commonly occurs in a group of posterior intestinal nuclei, and the pattern sometimes can be seen to extend to the anterior nuclei (though rarely). The variability in the expression pattern is not due to mosaicism as staining was performed on an integrated line."]
[5445,483,"yk354d12","Y47G6A.23","lpd-3","Expr2349","lpd-3::GFP transgenic worms, like lpd-1 (SREBP)::GFP worms, displayed prominent GFP expression in the intestine."]
[5456,1953,"yk53e12","Y46C8AL.1","-","Expr2066","Diffuse expression is seen in the anterior of the intestine in larvae and adults."]
[5556,2246,"yk499g4","C52D10.9","skr-8","Expr2442","Expression of SKR-8::GFP was detected only in the tip and end regions of the intestine."]
[6054,9271,"yk56d10","ZK524.3","lrs-2","Expr2105","Very weak diffuse expression is seen in the intestine of all larval stages and adults."]
[6066,6553,"yk577b4","C56G2.6","let-767","Expr2698","Transgenic animals carrying this construct express gfp in the gut, with a notable absence of expression in the gut nuclei. This pattern of expression is seen from the first larval stage through to adulthood in both hermaphrodites and males. GFP was not detected at any stage of embryogenesis."]
[6247,5116,"yk460e4","W03G9.4","app-1","Expr1652","Three independent lines of transformed nematodes expressing the W03G9.4::GFP transgene showed fluorescence that was entirely restricted to the intestinal cells of larval and adult stages. A common pattern in adult hermaphrodites was strong expression of GFP in the most anterior gut cells (Int 1) accompanied by less pronounced expression in the mid-gut and posterior-gut cells. The other frequently observed pattern, especially in larval stages, was equally strong expression in both the Int 1 cells and cells of the posterior region of the gut. The fluorescence was detected in the intestine from the L2 stage through to the adult, but no GFP expression was observed in embryos."]
[6357,9934,"yk225d5","F33D4.2","itr-1","Expr828","Expression of pC is observed in the intestine, pharyngeal isthmus and spermathecal (Sp) valve, uterine sheath cells and spermatheca."]
[6416,967,"yk501a5","ZK353.6","lap-1","Expr889","In all larval and adult stages stained cells are intestinal with some variation in the staining pattern between stages. Thus, in all stages, paired nuclei posterior to the pharyngealintestinal valve are stained and either paired or single nuclei along the length of the intestine. In adult worms, three paired rectal nuclei are stained which are not all seen in larval stages. That stained nuclei are in intestinal cells is indicated by distribution, size, shape and topology. Whereas LAP-1-lacZ transgenes are expressed along the length of the gut but not in the buccal cavity or pharynx, Indirect Fluorescent Antibody Tests using an antiserum specific for the protein show staining most intensely in the buccal cavity and rectal epithelium with staining also along the lining of the posterior pharyngeal bulb and in the in the anterior gut."]
[6470,7492,"yk557f6","B0213.4","nlp-29","Expr1694","Expressed in hypoderm, intestine."]
[6517,2334,"yk150d10","F22D6.12","gly-19","Expr1787","Expressed in intestine and anal sphinctor as soon as these structures appeared in the developing embryo and continued to be expressed until and include adulthood."]
[6679,325,"yk87g4","Y47D3B.7","sbp-1","Expr2348","Strong GFP expression, exclusively in the intestine."]
[6718,784,"yk305f9","B0414.2","rnt-1","Expr1819","Run is expressed in the lateral hypodermis and intestine of C. elegans. Reporter gene expression in embryos from all transformed lines was first detected exclusively in hypodermal seam cells at the bean stage of development. Reporter gene expression was observed in the nuclei of these cells, and expression persisted throughout embryogenesis. Expression of run in seam cell nuclei was detected up until the L3 stage. Between the late embryonic stage and L3 stage, expression was also apparent in gut cell nuclei, with the anterior ring of four cells (int-1) showing the highest level of expression. Expression of run in binucleated gut cells with large nuclei became evident at the L2 stage. At the L3 stage, expression of run in gut cells was significantly decreased except in those cells in the most anterior part of the intestine. Expression in seam cells remained virtually unchanged. By the L4 stage, expression of run was undetectable."]
[6863,10176,"yk603e2","F32B5.8","cpz-1","Expr2893","Beta-Galactosidase expression was observed in many cells, including gut, hypodermal, and other cells within the developing embryos. However, because of the intense staining in some parts of the developing embryos, the identification of the many stained cells was not possible. In post-embryonic stages, beta-galactosidase staining was present in the hypodermal cells of all larval stages mature gravid hermaphrodites (710 days after L4 adult hermaphrodite). Beta-Galactosidase staining was also detected in intestinal cells. The cpz-1:lacZ expression in all stages was specifically detected in the large hypodermal syncytium covering most of the worm (hyp7), and in the hypodermal cells of the head (hyp4 and hyp6) and occasionally in hypodermal cells in the tail. Beta-Galactosidase staining was also present in few pharyngeal cells of the adult worms but not in the pharyngeal cells of larval stages. Expression of cpz-1:gfp translational construct was detected in all developmental stages except embryos. The cpz-1:gfp expression was restricted to the hypodermis, with additional expression in the pharynx and the gonad only in the L4 and adult worms stages. The specific expression in the pharynx of adult stages was similar to that observed in cpz-1:lacZ transgenic worms. There was no embryonic expression of the gfp construct."]
[6896,673,"yk132g5","ZK524.3","lrs-2","Expr2105","Very weak diffuse expression is seen in the intestine of all larval stages and adults."]
[6970,2714,"yk271a8","C29E4.3","ran-2","Expr2038","Expression is confined to embryos and young larvae. In elongated embryos and young larvae diffuse expression is seen within the intestine. In pre-comma stage embryos a few nuclei (possibly intestinal precursors) show expression."]
[7048,7834,"yk562d8","C25A11.4","ajm-1","Expr1682","AJM-1 localizes to the apical borders of all C. elegans epithelia. Expression occurs in the embryonic hypodermis, pharynx and intestine, as well as in post-embryonic epithelia, including the vulva, uterus, spermathecae, pharynx, intestine, hindgut, hypodermis and male tail. MH27 staining of embryos expressing HMP-1GFP reveals that AJM-1 occupies an apical domain that is basal to the HMRHMP(cadherincatenin) complex in all epithelial tissues examined. Transmission electron microscopy (TEM) analysis of embryonic hypodermal cell junctions revealed an apical junctional domain resembling adherens junctions of flies and vertebrates, whereas no morphologically distinct septate or tight junctions are observed. The apicobasal extent of this domain is ~100 nm. Immuno-TEM analysis of hypodermal cells of larvae with the MH27 antibody revealed that AJM-1 specifically localizes to the apical domain."]
[7073,7992,"yk510c5","C25A11.4","ajm-1","Expr1682","AJM-1 localizes to the apical borders of all C. elegans epithelia. Expression occurs in the embryonic hypodermis, pharynx and intestine, as well as in post-embryonic epithelia, including the vulva, uterus, spermathecae, pharynx, intestine, hindgut, hypodermis and male tail. MH27 staining of embryos expressing HMP-1GFP reveals that AJM-1 occupies an apical domain that is basal to the HMRHMP(cadherincatenin) complex in all epithelial tissues examined. Transmission electron microscopy (TEM) analysis of embryonic hypodermal cell junctions revealed an apical junctional domain resembling adherens junctions of flies and vertebrates, whereas no morphologically distinct septate or tight junctions are observed. The apicobasal extent of this domain is ~100 nm. Immuno-TEM analysis of hypodermal cells of larvae with the MH27 antibody revealed that AJM-1 specifically localizes to the apical domain."]
[7125,9424,"yk541f2","Y46C8AL.1","-","Expr2066","Diffuse expression is seen in the anterior of the intestine in larvae and adults."]
[7170,5618,"yk613c6","ZK455.1","aco-1","Expr434","Expression is seen from early embryonic stages to adulthood. In embryos many cells show expression including the intestinal precursors. In young larvae expression is confined to the intestine, but in older worms more extensive expression is seen, including many hypodermal cells. This gene has homology to aconitase."]
[7424,4367,"yk320d3","B0395.1","nhx-1","Expr2232","When GFP expression was driven by the promoter sequences upstream of the NHX-1-coding sequences, fluorescence was found nearly exclusively in the intestine of the worm. The entire intestine was labeled evenly in the nhx-1::GFP transgenic animals; however, the nhx-1::GFP labeling was most likely an artifact according to the results obtained from GFP translational construct. See Expr"]
[7431,5096,"yk433c2","Y75B8A.4","-","Expr437","Expression is seen in the intestine from three fold embryonic to adult stages. Expression is generally weak, being both nuclear localised and diffuse throughout the cells, and occasionally having a granular appearance."]
[7501,2454,"yk385c7","C46H11.4","lfe-2","Expr505","Pharynx & gut in L1 through adult; spermathecae in adult."]
[7579,1558,"yk437e2","F33D4.2","itr-1","Expr828","Expression of pC is observed in the intestine, pharyngeal isthmus and spermathecal (Sp) valve, uterine sheath cells and spermatheca."]
[7652,5705,"yk87g4","Y47D3B.7","sbp-1","Expr2348","Strong GFP expression, exclusively in the intestine."]
[7681,6992,"yk658h11","C56G2.6","let-767","Expr2698","Transgenic animals carrying this construct express gfp in the gut, with a notable absence of expression in the gut nuclei. This pattern of expression is seen from the first larval stage through to adulthood in both hermaphrodites and males. GFP was not detected at any stage of embryogenesis."]
[7731,4737,"yk386b4","C08B11.4","nrf-6","Expr1214","Expressed strong GFP fluorescence in hyp 3 and hyp 5, the most anterior cells in the hypodermis, and in the intestine. This expression began at the L1 stage and continued throughout adulthood. Weaker fluorescence was also observed in other hypodermal cells throughout the animal, particularly in later stages of development."]
[7746,7217,"yk578b11","K08E7.9","pgp-1","Expr710","Staining of large nuclei localized alongside intestine, posteriorly of the pharyngo-intestinal valve and anteriorly of the anus. pPD26pgp3.BP strains are detectable in embryonic intestinal cells. pPD26pgp1.X stains with low intensity. Most uniform staining seen in early larval stages. The fraction of L3 and L4 and adult animals that are stained is smaller and staining is of less intensity. Nuclei of anterior-most ring of four cells and of the posterior four-six cells are usually more heavily stained than nuclei of other cells."]
[7760,4392,"yk315f11","ZK455.7","pgp-3","Expr749","Staining of large nuclei localized alongside intestine, posteriorly of the pharyngo-intestinal valve and anteriorly of the anus. In some experiments staining of a few parapharyngeal nuclei in 1/10 transgene (pPd26pgp3.XX) is observed. Reproducible staining is limited to the intestine with some variation in the number of cells that stain and in intensity. pPD26pgp3.XX strains are detectable in embryonic intestinal cells. Most uniform staining seen in early larval stages. The fraction of L3 and L4 and adult animals that are stained is smaller and staining is of less intensity. Nuclei of anterior-most ring of four cells and of the posterior four-six cells are usually more heavily stained than nuclei of other cells."]
[7761,2463,"yk357b7","Y116A8C.33","-","Expr174","Weak nuclear expression is observed in the posterior intestinal cells of larval and adult stage worms. Occasionally, more extensive expression throughout the intestine is seen."]
[7863,9792,"yk560e8","C33D3.1","elt-2","Expr1216","Only the large gut nuclei detected."]
[7865,7633,"yk9a7","K08E7.9","pgp-1","Expr710","Staining of large nuclei localized alongside intestine, posteriorly of the pharyngo-intestinal valve and anteriorly of the anus. pPD26pgp3.BP strains are detectable in embryonic intestinal cells. pPD26pgp1.X stains with low intensity. Most uniform staining seen in early larval stages. The fraction of L3 and L4 and adult animals that are stained is smaller and staining is of less intensity. Nuclei of anterior-most ring of four cells and of the posterior four-six cells are usually more heavily stained than nuclei of other cells."]
[7913,8820,"yk571a9","F38A6.1","pha-4","Expr509","In the gut at the 8E cell stage; E descendants continue to show expression through late embryo. Pharyngeal cells derived from both AB and MS lineages show staining starting at comma stage. Intestinal and pharyngeal staining decline after hatching. The developing somatic gonad shows expression at L3 and L4. In comma to 1.5-fold stage embryos, hybridization was also seen in the tail region."]
[8016,6032,"yk462c9","ZK455.1","aco-1","Expr434","Expression is seen from early embryonic stages to adulthood. In embryos many cells show expression including the intestinal precursors. In young larvae expression is confined to the intestine, but in older worms more extensive expression is seen, including many hypodermal cells. This gene has homology to aconitase."]
[8046,4476,"yk430c10","K02D7.4","dsc-4","Expr2799","dsc-4::gfp transcriptional and rescuing translational reporter genes are expressed in the intestine from early embryogenesis, just after the beginning of elongation, throughout larval stages and adulthood."]
[8067,1880,"yk431a1","F38B6.4","-","Expr104","Strong expression is seen in the large posterior nuclei of the intestine, in adults and late larval stages. There is some variability in the number of nuclei staining. Usually the extreme posterior nuclei stain first. Occasionally in 3-fold embryos a few nuclei show b-galactosidase expression. All injected lines show this expression. This fusion contains all the intergenic region, though this region is only separated from F38B6.5 (the next upstream gene) by ~0.5kb, and so could be a downstream gene; the promoter driving expression of F38B6.4 would then not be included in the construct."]
[8135,1024,"yk136h7","T03E6.7","cpl-1","Expr1775","Expression of the lacZ transcriptional fusion construct was detected in all developmental stages, from early embryos through to the adult stages. In embryos, beta-galactosidase expression was confined exclusively to gut cells. In larvae and adults, both hermaphrodites and males, strong expression was also detected in all gut cells. In addition, beta-galactosidase staining was also observed in hypodermal cells of many larval and adult transgenic worms, although the number of hypodermal cells showing expression and the level of expression was more mosaic than gut cell expression. Examination of transformants with high levels of hypodermal cell expression showed that all types of hypodermal cells, dorsal, lateral, and ventral cells, expressed the cpl-1:lacZ transgene."]
[8226,2276,"yk30a5","K09C8.1","pbo-4","Expr2235","The nhx-7::GFP reporter resulted in fluorescence specifically and exclusively in the posterior cells of the intestine. Similarly, GFP expression was restricted primarily to a single cell or cell type in a surprisingly large number of transgenic strains."]
[8262,3917,"yk122b6","H22K11.1","asp-3","Expr2132","Weak expression is seen throughout the intestines of older larvae and adults. Expression is generally cytoplasmic and excluded from intestinal cell nuclei. Weak cytoplasmic expression is also seen within the uterus of adult worms. This gene has homology to aspartyl proteases."]
[8314,4051,"yk364c6","K11G9.6","mtl-1","Expr1508","In uninduced animals, mtl-1::lacZ was active in just three cells that are located in the posterior bulb of pharynx."]
[8335,2757,"yk369f3","C52E4.1","cpr-1","Expr621","High level of gut cell staining in all developmental stages except embryos. There was variability in intensity and the number of gut cells staining between individuals of the same transgenic lines. A small number of transformants within each line showed expression in all gut cells but the majority showed staining restricted to the most anterior (Int1) the mid anterior (Int2 and Int3) and the mid gut cells (Int4, 5 and 6). Both intensity and frequency of expression decreased from the most anterior to the mid-gut region. A few worms stained only in posterior gut cells."]

{"sheet":"X box","index":4,"n_rows":16,"n_cols":3,"rows_kept":16,"merged":[[0,0,0,6]],"flags":["formulas_cached_only"]}
["genes that carry X-boxes in promoter regions"]
["rank","CDS","locus"]
[2,"C33A12.4","-"]
[20,"C27A7.4","che-11"]
[54,"C32E8.3","-"]
[71,"B0491.4","-"]
[113,"C39D10.5","-"]
[448,"W02B12.3","rsp-1"]
[1263,"T19A5.4","nhr-44"]
[1590,"K04B12.2","-"]
[2224,"K08F8.1","-"]
[2619,"R13G10.2","amx-1"]
[3362,"F29B9.8","-"]
[4811,"ZK863.4","-"]
[7284,"C27F2.1","-"]
[7434,"C43C3.3","dyf-8"]