MutantSample_3_R1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	MutantSample_3_R1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100000
Sequences flagged as poor quality	0
Sequence length	50
%GC	49

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	879	0.8789999999999999	No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG	504	0.504	No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	311	0.311	No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA	304	0.304	No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA	252	0.252	No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG	250	0.25	No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG	217	0.217	No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC	201	0.201	No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC	196	0.196	No Hit
CGAACTCTCCAGAGGTGGCAACTGGCCTCAGACACCATGGCACCAAAGAA	174	0.174	No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA	112	0.11199999999999999	No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC	106	0.106	No Hit
CGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGATCA	104	0.104	No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT	103	0.10300000000000001	No Hit

Can't analyse adapters as read length is too short

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTCTA	25	4.4035834E-5	44.01701	2
TACGGTA	25	4.416619E-5	43.995	44
TACCTTG	30	2.5064764E-6	43.995	9
TTACGGT	25	4.416619E-5	43.995	43
AGGGAAC	35	7.2293824E-6	37.71	42
AGTCTAA	30	1.289676E-4	36.68084	3
CCTTACG	30	1.2934812E-4	36.6625	41
CTTACGG	30	1.2934812E-4	36.6625	42
CAGTTAC	40	1.7987428E-5	33.012756	5
GTTACCT	40	1.8049173E-5	32.99625	7
TTACCTT	40	1.8049173E-5	32.99625	8
ACCTTGT	40	1.8049173E-5	32.99625	10
TCCCTTA	35	3.1990936E-4	31.425	39
GGGAACC	35	3.1990936E-4	31.425	43
CCCTTAC	35	3.1990936E-4	31.425	40
GGAGTCT	50	2.2898312E-6	30.873684	1
AGTTACC	45	4.0221326E-5	29.344673	6
CTTGTTT	45	4.0358995E-5	29.33	12
AAGTTGG	30	0.0057233507	29.33	43
AAAGTTC	40	6.9914607E-4	27.496876	22