## File locations
REF_FILE_NAME   NCBI_data/GCF_000001215.4_Release_6_plus_ISO1_MT_genomic_modified.gtf
GEN_DIR         Genome/

## Library preparation
# Expression
NB_MOLECULES    50000000
TSS_MEAN	50
POLYA_SCALE     NaN
POLYA_SHAPE     NaN

# Fragmentation
FRAG_SUBSTRATE	RNA
FRAG_METHOD	UR
FRAG_UR_ETA     350

# Reverse Transcription
RTRANSCRIPTION	YES
RT_PRIMER	RH
RT_MOTIF	default
RT_MIN		300

# Amplification 
PCR_DISTRIBUTION default
GC_MEAN 	 NaN
PCR_PROBABILITY  0.05

# Size Filtering
FILTERING 	YES
SIZE_DISTRIBUTION	N(400, 50)
SIZE_SAMPLING	AC

## Sequencing
READ_NUMBER	100000000
READ_LENGTH	100
PAIRED_END	YES
FASTA		TRUE
ERR_FILE	76