SEAseq Report

LEGEND
EXCELLENT
GOOD
AVERAGE
BELOW-AVERAGE
POOR

Sample FASTQs Quality Results

Sample Name	Overall Quality	Raw Reads	Read Length	Base Quality	Sequence Diversity	Aligned Percent	NRF	PBC	NSC	RSC
SRR10259398	GOOD	54894988	75	pass	pass	80.787	0.8183	0.8378	1.0266	0.8804

Control FASTQs Quality Results

Sample Name	Overall Quality	Raw Reads	Read Length	Base Quality	Sequence Diversity	Aligned Percent	NRF	PBC	NSC	RSC
SRR10259397	GOOD	45603300	75	pass	pass	78.713	0.9425	0.9713	1.0132	0.7821

Overall Quality Evaluation and Statistics Results

DATA Overall Quality Raw Reads Base Quality Sequence Diversity Aligned Percent Estimated Fragment Width Estimated Tag Length NRF PBC NSC RSC FRiP Total Peaks Normalized Peaks* Linear Stitched Peaks SE-like Enriched Regions

SAMPLE GOOD 56053120 pass pass 79.118 121 75 0.8183 0.8378 1.0266 0.8804 0.0218 11248 11248 540 52

CONTROL 46608378 pass pass 77.016 200 75 0.9425 0.9713 1.0132 0.7821 0.0015 605

DATA	Overall Quality	Raw Reads	Base Quality	Sequence Diversity	Aligned Percent	Estimated Fragment Width	Estimated Tag Length	NRF	PBC	NSC	RSC	FRiP	Total Peaks	Normalized Peaks*	Linear Stitched Peaks	SE-like Enriched Regions
SAMPLE	GOOD	56053120	pass	pass	79.118	121	75	0.8183	0.8378	1.0266	0.8804	0.0218	11248	11248	540	52
CONTROL	46608378	pass	pass	77.016	200	75	0.9425	0.9713	1.0132	0.7821	0.0015	605

* Peaks identified after Input/Control correction.

Abbreviations (adopted from Landt et al, Genome Res. 2012)

NRF : Non-Redundant Fraction.
PBC : PCR BottleNeck Coefficient.
NSC : Normalized Strand Cross-correlation coefficient.
RSC : Relative Strand Cross-correlation coefficient.
FRiP : Fraction of Reads in Peaks.

Definitions for all metrics can be found on the SEAseq website.

SEAseq Quality Statistics and Evaluation Report

Sample FASTQs Quality Results

Control FASTQs Quality Results

Overall Quality Evaluation and Statistics Results