Table S1. Genotypes of the studied Spanish Brachypodium distachyon individuals based on nSSR analysis. Individual\locus ALB006 ALB022 ALB040 ALB050 ALB086 ALB087 ALB139 ALB165 ALB181 ALB311 Pop ALM ALM1 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM2 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM3 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM4 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM5 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM6 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM7 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM8 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM9 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM10 366:366 354:354 182:182 225:225 190:190 192:192 308:308 195:195 238:238 250:250 ALM11 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM12 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 ALM13 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 Pop GRAZ GRAZ14 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 GRAZ15 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 GRAZ16 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 GRAZ17 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 GRAZ18 366:366 354:354 182:182 225:225 190:190 192:192 308:308 195:195 238:238 250:250 GRAZ19 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 GRAZ20 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 GRAZ21 366:366 354:354 182:182 221:221 190:190 192:192 308:308 195:195 238:238 250:250 Pop VAL VAL22 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 VAL23 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 VAL24 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 VAL25 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 VAL26 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 VAL27 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 VAL28 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 VAL29 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 Pop LEO LEO30 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO31 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO32 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO33 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO34 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO35 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO36 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO37 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO38 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 LEO39 360:360 356:356 182:182 217:217 196:196 202:202 308:308 201:201 238:238 246:246 Pop OUR OUR40 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 OUR41 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 OUR42 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 OUR43 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 OUR44 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 OUR45 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 OUR46 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 OUR47 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 OUR48 360:360 356:356 182:182 217:217 196:196 202:202 308:308 191:191 238:238 246:246 Pop ZAR ZAR49 368:368 358:358 176:176 231:231 198:198 200:200 310:310 179:179 238:238 246:246 ZAR50 374:374 358:358 176:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 ZAR51 368:368 358:358 176:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 ZAR52 368:368 358:358 176:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 ZAR53 374:374 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 ZAR54 374:374 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 ZAR55 374:374 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 ZAR56 360:360 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 ZAR57 360:360 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 Pop HUE1 HUE158 360:360 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE159 360:360 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE160 360:360 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE161 360:360 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE162 360:360 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 HUE163 360:360 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE164 360:360 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE165 360:360 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 HUE166 360:360 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE167 360:360 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 Pop HUE2 HUE268 368:368 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE269 368:368 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE270 368:368 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE271 368:368 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 HUE272 368:368 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE273 368:368 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE274 368:368 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE275 368:368 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 HUE276 368:368 358:358 182:182 231:231 198:198 200:200 310:310 179:179 238:238 246:246 HUE277 368:368 358:358 182:182 231:231 198:198 200:200 310:310 173:173 238:238 246:246 Pop HUE3 HUE378 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE379 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE380 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE381 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE382 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE383 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE384 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE385 368:368 358:358 182:182 227:227 198:198 200:200 310:310 179:179 238:238 246:246 HUE386 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE387 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 Pop HUE4 HUE488 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE489 368:368 358:358 182:182 227:227 198:198 200:200 310:310 179:179 238:238 246:246 HUE490 368:368 358:358 182:182 227:227 198:198 200:200 310:310 179:179 238:238 246:246 HUE491 368:368 358:358 182:182 227:227 198:198 200:200 310:310 179:179 238:238 246:246 HUE492 368:368 358:358 182:182 227:227 198:198 200:200 310:310 179:179 238:238 246:246 HUE493 368:368 358:358 182:182 227:227 198:198 200:200 310:310 179:179 238:238 246:246 HUE494 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE495 368:368 358:358 182:182 227:227 198:198 200:200 310:310 179:179 238:238 246:246 HUE496 368:368 358:358 182:182 227:227 198:198 200:200 310:310 179:179 238:238 246:246 HUE497 368:368 358:358 182:182 225:225 198:198 200:200 310:310 173:173 238:238 246:246 Pop HUE5 HUE598 368:368 358:358 182:182 225:225 198:198 200:200 310:310 173:173 238:238 246:246 HUE599 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE5100 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE5101 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE5102 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE5103 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE5104 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE5105 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE5106 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 HUE5107 368:368 358:358 182:182 227:227 198:198 200:200 310:310 173:173 238:238 246:246 Pop NAV1 NAV1108 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1109 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1110 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1111 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1112 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1113 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1114 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1115 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1116 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV1117 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 Pop NAV2 NAV2118 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2119 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2120 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2121 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2122 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2123 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2124 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2125 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2126 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 NAV2127 368:368 358:358 182:182 225:225 198:198 200:200 310:310 179:179 234:234 246:246 Pop LLE1 LLE128 368:368 358:358 178:178 227:227 198:198 200:200 310:310 191:191 234:234 244:244 LLE129 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244 LLE130 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244 LLE131 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244 LLE132 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244 LLE133 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244 LLE134 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244 LLE135 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244 LLE136 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244 LLE137 368:368 358:358 178:178 225:225 198:198 200:200 310:310 191:191 234:234 244:244