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["Primers for mutant construction "]
["Primer name","Nucleotide sequencea","Restriction enzyme"]
["Y-UP-S","CG GAATTC GCACCAATTCGCTC","EcoRI"]
["Y-UP-A","CG GGATCC TTGTCGTCGCCCGT","BamH I"]
["Y-DN-S","CG GGATCC ACCGCACCCGTC","BamH I"]
["Y-DN-A","GC TCTAGA CCACGCCCGCACCTA","XbaI"]
["F-UP-S","G GAATTC GGGTTCTTCGGCATG","EcoRI"]
["F-UP-A","GC TCTAGA GTAGGGCAGGAGGTG","XbaI"]
["F-DN-S","GC TCTAGA TTTTCGACCGATCCGAAC","XbaI"]
["F-DN-A","CCC AAGCTT CCGTCGGCCAGCACG","HindIII"]
["M-UP-S","G GAATTC CGTCACCTCGGCAATCC","EcoRI"]
["M-UP-A","GC TCTAGA CTGGCCGCCTGCATC","XbaI"]
["M-DN-S","GC TCTAGA GCTGAACTCCGCGATC","XbaI"]
["M-DN-A","CCC AAGCTT CGCCGGTCTGAAATC","HindIII"]
[]
[]
["primers for complementation test"]
["Primer name","Nucleotide sequencea","Restriction enzyme"]
["CY-S","CG GGATCC ATGACTGCAGGCATCGAGGGAC","BamH I"]
["CY-A","GC TCTAGA TCAGTTCAGGGTGAAGCTGAG","XbaI"]
["CF-S","CG GGATCC ATGGCCGAAGTAAAATCCGACA","BamH I"]
["CF-A","GC TCTAGA TTAGAACAGCCCTTCGATCTGG","XbaI"]
["CM-S","CG GGATCC ATGTCGAGCGCCGTGGAAAAGC","BamH I"]
["CM-A","GC TCTAGA  CTAAGGATCGCGGAGTTCAGCA","XbaI"]
[]
[]
["a restriction sites are shown in talics"]

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